Abstract:
:Plant secondary metabolites including alkaloids, demonstrate a complex diversity in their molecular scaffolds and exhibit tremendous pharmacological potential as anti-cancerous therapeutics. The present study aimed to evaluate the anticancer activity of a natural alkaloid, mecambridine, against human oral squamous cell carcinoma (OSCC). An MTT assay was used to evaluate cytotoxic effects of mecambridine on HSC-3 oral squamous cell carcinoma cells. Effects of mecambridine on autophagy-associated proteins were analyzed by western blotting. Effects on reactive oxygen species (ROS) and mitochondrial membrane potential were assessed by flow cytometry. Results indicated that mecambridine exhibited an IC50 value of 50 µM and exerted its cytotoxic effects in a dose dependent manner on OSCC HSC-3 cells. Furthermore, it was observed that mecambridine decreases cell viability and induces autophagy in a dose-dependent manner. The underlying mechanism for the induction of autophagy was demonstrated to be associated with ROS-mediated alterations in mitochondrial membrane potential and modulation of the mechanistic target of rapamycin/phosphoinositide 3-kinase/protein kinase B (m-TOR/PI3K/Akt) signaling pathway in HSC-3 at the IC50. In conclusion, the present study suggests that mecambridine exhibits substantial anticancer activity against OSCC HSC-3 cells by induction of autophagy and modulates the expression of the mTOR/PI3K/Akt signaling cascade which is considered a potential target pathway for anti-cancer agents.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Lin N,Li Z,Wang D,Zheng K,Wu Y,Wang Hdoi
10.3892/ol.2017.7321subject
Has Abstractpub_date
2018-01-01 00:00:00pages
292-296issue
1eissn
1792-1074issn
1792-1082pii
OL-0-0-7321journal_volume
15pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Tumor-initiating cells (TICs) are defined as a specialized subset of cells with tumor-initiating capacity that can initiate tumor growth, tumor relapse and metastasis. In the present study, osteosarcoma TICs (OS-TICs) were isolated and enriched from the osteosarcoma U2OS and MG-63 cell lines using sphere formation ass...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6132
更新日期:2017-07-01 00:00:00
abstract::Septin 2 (SEPT2) is a tumor-related gene belonging to the SEPT family that affects the cellular processes of hepatoma carcinoma cells, glioblastoma cells and mesangial cells and is highly expressed in breast cancer, biliary tract cancer and acute myeloid leukemia. Colorectal cancer (CRC) is the third most common type ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10528
更新日期:2019-09-01 00:00:00
abstract::Liver kinase B1 (LKB1) encodes a serine/threonine kinase and functions as a tumor suppressor. LKB1 loss-of-function somatic mutations are frequently observed in sporadic types of cancer, particularly in lung cancer. Ectopic LKB1 induces growth arrest by upregulating p21/cyclin dependent kinase inhibitor 1A (WAF1) in L...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8741
更新日期:2018-07-01 00:00:00
abstract::Metastatic spread of gastric carcinoma to the breast is rare. In previous decades, reports on this subject were minimal and primarily limited to case reports. At present, little is known on the clinicopathological features and prognosis of this condition, and breast metastasis remains a challenging clinical problem. A...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9383
更新日期:2018-11-01 00:00:00
abstract::The present study aimed to assess the feasibility, safety and efficiency of ultrasound-guided percutaneous microwave ablation (MWA) on liver metastases from colon or rectal cancer. Patients who received MWA therapy for liver metastases from colon or rectal cancer between June 2009 and May 2012 were enrolled in the stu...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2106
更新日期:2014-07-01 00:00:00
abstract::Increasing numbers of extrapleural solitary fibrous tumours (SFTs), including the prostate SFT, have been reported over the last 10-years. Prostate SFT is relatively uncommon, with <20 cases reported in the literature worldwide. In the present study, a prostate SFT case, which was initially misdiagnosed as benign pros...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3379
更新日期:2015-09-01 00:00:00
abstract::The epithelial cell adhesion molecule (EpCAM) is overexpressed in the majority of human epithelial carcinomas, and its overexpression is associated with proliferation and neoplastic transformation. However, the precise molecular mechanism involved in EpCAM-related proliferation and metastasis in hypopharyngeal carcino...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2140
更新日期:2014-07-01 00:00:00
abstract::Central diabetes insipidus (DI) is a rare complication in patients with acute myeloid leukemia (AML), typically occurring in patients with abnormalities of chromosomes 3 or 7. The association between AML with monosomy 7 and DI has been described in a number of studies; however, DI has been rarely reported in cases of ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3139
更新日期:2015-06-01 00:00:00
abstract::The expression of human transfer ribonucleic acid (tRNA) methyltransferase 9-like (KIAA1456) protein in lung cancer tissue and its effects on certain genes involved in the proliferation, migration and invasion of lung cancer cells were investigated. Immunohistochemistry was applied to stain lung cancer tissue and adja...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9119
更新日期:2018-09-01 00:00:00
abstract::Previous studies have revealed the aberrant expression of a number of microRNAs (miRNA/miRs) in the blood circulation of patients with breast cancer (BC). The aim of the present study was to assess the effect of neoadjuvant chemotherapy on the levels of a panel of BC-associated miRNAs, which are at relatively low (let...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2188
更新日期:2014-08-01 00:00:00
abstract::Effect of microRNA-27b (miR-27b) on cisplatin chemotherapy sensitivity of oral squamous cell carcinoma (OSCC) was investigated to provide a reference for clinical prevention of OSCC cell resistance. The clinical tissues of 34 patients with OSCC-resistant cancer and 28 patients with cisplatin-sensitive OSCC in Nanjing ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10347
更新日期:2019-07-01 00:00:00
abstract::The aim of the present study was to perform a retrospective analysis to investigate the outcome and toxicity of radiation (RT) and chemoradiation (CRT) in elderly, inoperable patients >70 years old. Between 2003 and 2012, 1,024 patients with squamous cell carcinoma (SCC) of the esophagus were treated at the Department...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1694
更新日期:2014-01-01 00:00:00
abstract::The present study aims to quantitatively measure adiposity-related image features and to test the feasibility of applying multivariate statistical data analysis-based prediction models to generate a novel clinical marker and predict the benefit of epithelial ovarian cancer (EOC) patients with and without maintenance b...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4648
更新日期:2016-07-01 00:00:00
abstract::Cancer and embryo expression protein 65 (CEP65) is a centrosomal protein that is expressed at relatively high levels in embryonic tissue and different cancerous tissues, but its role in tumorigenesis remains unknown. In the present study, CEP65 was stably expressed in AGS gastric cancer cells. CEP65 was found to promo...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.2958
更新日期:2015-04-01 00:00:00
abstract::[This corrects the article DOI: 10.3892/ol.2018.8006.]. ...
journal_title:Oncology letters
pub_type: 已发布勘误
doi:10.3892/ol.2020.12110
更新日期:2020-11-01 00:00:00
abstract::Ubiquitin-like modifier-activating enzyme 7 (UBA7) is a specific E1-like ubiquitin-activating enzyme involved in interferon-stimulated gene 15 (ISG15) conjugation. UBA7 expression has been reported to be notably decreased in lung cancer. The present study aimed to investigate the changes in UBA7 expression in breast c...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11406
更新日期:2020-04-01 00:00:00
abstract::Aberrant promoter methylation of tumor relevant genes frequently occurs in early steps of carcinogenesis and during tumor progression. Epigenetic alterations could be used as potential biomarkers for early detection and for prediction of prognosis and therapy response in lung cancer. The present study quantitatively a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4927
更新日期:2016-10-01 00:00:00
abstract::The aim of this study was to construct the eukaryotic expression vector pEGFP-N1-hPer2 and assess its expression in the human osteosarcoma cell line MG63. Total mRNA was extracted from human osteosarcoma MG63 cells, the human period 2 (hPer2) gene was obtained by reverse transcription-polymerase chain reaction (RT-PCR...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4291
更新日期:2016-04-01 00:00:00
abstract::The issue of delayed neurological damage as a result of treatment is becoming increasingly important now that an increased number of children survive treatment for acute lymphoblastic leukemia (ALL). Following modification of the treatment protocols, severe symptomatic late effects are rare, and most adverse effects a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1072
更新日期:2013-02-01 00:00:00
abstract::Expression level of miR-181c in neuroblastoma children and its effect on proliferation of neuroblastoma M17 cells were investigated. Fifty-seven neuroblastoma patients admitted to Weifang People's Hospital from January 2013 to December 2017 were selected and their cancer tissues and normal adjacent tissues were obtain...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10602
更新日期:2019-09-01 00:00:00
abstract::The aim of the present study was to investigate whether an increase in cyclin-dependent kinase 2 (CDK2) activity is involved in apoptosis of human bladder cancer T24 cells induced by isoliquiritigenin (ISL). The viability of T24 cells was estimated using a sulforhodamine B assay. Cell morphological changes were examin...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6159
更新日期:2017-07-01 00:00:00
abstract::Phospholipase C (PLC) is a membrane-associated enzyme that regulates several cellular behaviors including cell motility, growth, transformation and differentiation. PLC is involved in cancer migration, invasion and drug resistance. However, the expression status and prognostic role of PLCB4 in acute myeloid leukemia (...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10921
更新日期:2019-12-01 00:00:00
abstract::Breast cancer is a highly heterogeneous disease at the molecular level and >90% of mortalities are due to metastasis and its associated complications. The present study determined the impact of molecular subtypes on metastatic behavior and overall survival (OS) of patients with metastatic breast cancer. The influence ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11948
更新日期:2020-10-01 00:00:00
abstract::Current therapy for rheumatoid arthritis (RA) relies on global suppression of the immune response or specific blockade of inflammatory cytokines. However, it is unclear how immunosuppressants affect patients with cancer. Therefore, in the present study, the effect of three biological agents, tofacitinib, anti-mouse IL...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6473
更新日期:2017-09-01 00:00:00
abstract::The purpose of this study was to present the results of fertility-sparing treatment using medroxyprogesterone acetate (MPA) for endometrial carcinoma (EC), and to clarify patient characteristics by investigating patient background factors. A total of 59 patients with EC, who received MPA as fertility-sparing therapy a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.602
更新日期:2012-05-01 00:00:00
abstract::Ovarian cancer is the most fatal gynecological cancer, with a 5-year survival rate of only 30%. Lysophosphatidic acid (LPA), which possesses growth factor-like functions, is a major regulatory factor in the peritoneal metastasis of ovarian cancer. LPA stimulates the expression of numerous genes that are associated wit...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.1926
更新日期:2014-05-01 00:00:00
abstract::Renal oncocytoma is one of the most unusual benign lesion, which are usually diagnosed postoperatively, since their differentiation from renal cell carcinoma is challenging. The present study reports two cases of renal oncocytoma in a 60-year-old woman and a 46-year-old man. Relevant clinical and pathological data on ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4594
更新日期:2016-07-01 00:00:00
abstract::Rare types of cancer are often not effectively treated by approaches such as chemotherapy and radio-therapy, although their side-effects persist. Immunotherapy has been gaining attention worldwide with growing examples of its anticancer activity demonstrated in vivo. This case report describes a 35-year-old male who s...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1247
更新日期:2013-05-01 00:00:00
abstract::A 62-year-old male patient underwent endoscopic mucosal resection (EMR). Additional hybrid 2-port hand-assisted laparoscopic surgery (HALS) (Mukai's operation) was performed for early rectal cancer located at the distal border of the rectum/below the peritoneal reflection (Rb) region [SM massive invasion/ly+/vertical ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2011.327
更新日期:2011-09-01 00:00:00
abstract::Deoxypodophyllotoxin (DPPT) is extracted and separated from citrus-related plants, including Podophyllum (P.) peltatum, P. pleianthum, P. emodi (also called P. hexandrum) and Diphylleia grayi. DPPT has significant antitumor and antiviral activity. However, due to its strong toxicity and side effects, its use is limite...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4943
更新日期:2016-10-01 00:00:00