Abstract：
:Chemokines are guiding cues for directional trafficking of mesenchymal stem cells (MSC) upon injury and local chemokine delivery at injury sites is an up-to-date strategy to potentiate and prolong recruitment of MSC. In this study we present the chemokine CCL25, also referred to as thymus-expressed chemokine, to mobilize human MSC along positive but not along negative gradients. We hence proceeded to design a biodegradable and injectable release device for CCL25 on the basis of poly(lactic-co-glycolic acid) (PLGA). The conducted studies had the objective to optimize PLGA microparticle fabrication by varying selected formulation parameters, such as polymer type, microparticle size and interior phase composition. We found that microparticles of DV,50∼75 µm and fabricated using end-capped polymers, BSA as carrier protein and vortex mixing to produce the primary emulsion yielded high chemokine loading and delayed CCL25 release. To determine bioactivity, we investigated CCL25 released during the microparticle erosion phase and showed that deacidification of the release medium was required to induce significant MSC mobilization. The designed PLGA microparticles represent an effective and convenient off-the-shelf delivery tool for the delayed release of CCL25. However, continuative in vivo proof-of-concept studies are required to demonstrate enhanced recruitment of MSC and/or therapeutical effects in response to CCL25 release microparticles. STATEMENT OF SIGNIFICANCE:With the discovery of chemokines, particularly CXCL12, as stimulators of stem cell migration, the development of devices that release CXCL12 has proceeded quickly in the last few years. In this manuscript we introduce CCL25 as chemokine to induce mobilization of human MSC. This study proceeds to demonstrate how selection of key formulation parameters of CCL25 loading into PLGA microparticles exerts considerable influence on CCL25 release. This is important for a broad range of efforts in in situ tissue engineering where the candidate chemokine and the delivery device need to be selected carefully. The use of such a cell-free CCL25 release device may provide a new therapeutic option in regenerative medicine.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Fröhlich K,Hartzke D,Schmidt F,Eucker J,Gurlo A,Sittinger M,Ringe Jdoi
10.1016/j.actbio.2018.01.036subject
Has Abstractpub_date
2018-03-15 00:00:00pages
290-300eissn
1742-7061issn
1878-7568pii
S1742-7061(18)30047-3journal_volume
69pub_type
杂志文章abstract：:The physical and mechanical properties of structural proteins such as silk fibroin can be modified by controlled conformational change, which is regularly monitored by Fourier transform infrared spectroscopy by peak fitting of the amide I band envelope. Although many variables affecting peak shape are well established...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.03.058
更新日期：2018-06-01 00:00:00
abstract：:Several studies have reported the benefits of mesenchymal stem cells (MSCs) for bone tissue engineering. However, vascularization remains one of the main obstacles that must be overcome to reconstruct large bone defects. In vitro prevascularization of the three-dimensional (3-D) constructs using co-cultures of human p...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.05.025
更新日期：2013-09-01 00:00:00
abstract：:Vascular grafts are widely used for a number of medical treatments. Strength, compliance, endothelialization and availability are issues of most concern for vascular graft materials. With current approaches, these requirements are difficult to satisfy simultaneously. To explore an alternative approach, the present stu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.09.028
更新日期：2010-04-01 00:00:00
abstract：:Chitosan membranes were modified with mouse epidermal growth factor (EGF) by a photochemical technique. Photochemical immobilization was performed via a two-step process, in which EGF was first reacted with a heterobifunctional cross-linker sulfo-SANPAH (sulfosuccinimidyl 6(4'-azido-2'-nitrophenyl-amino)hexanoate) and...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.02.003
更新日期：2008-07-01 00:00:00
abstract：:Antimicrobial peptides (AMPs) are currently receiving interest as an alternative to conventional antibiotics to treat biomaterial-associated infection. However, the inherent instability of such peptides often limits their efficacy in intended clinical applications. Covalent immobilization of AMPs to surfaces is one st...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.10.039
更新日期：2019-01-01 00:00:00
abstract：:Synthetic three-dimensional scaffolds for cell and tissue engineering routinely utilize peptide ligands to provide sites for cell adhesion and to promote cellular activity. Given the fact that recent studies have dedicated great attention to the mechanisms by which cell behavior is influenced by various ligands and sc...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.03.040
更新日期：2010-09-01 00:00:00
abstract：:Natural silk fiber spun by the silkworm Bombyx mori is widely used not only for textile materials, but also for biofunctional materials. In the present study, we genetically engineered an advanced silk material, named hSFSV, using a transgenic silkworm, in which the recombinant human acidic fibroblast growth factor (h...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.06.031
更新日期：2014-12-01 00:00:00
abstract：:This work demonstrates the effects of both surface preparation and surface post-treatment by exposure to electron beam on the surface texture, contact angle and the interaction with bone-forming cells of electrochemically deposited hydroxyapatite (HAp) coating. Both the surface texture and the contact angle of the gro...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.04.005
更新日期：2009-10-01 00:00:00
abstract：:The main aim of this review study was to report the state of art on the nano-scale technological advancements of titanium implant surfaces to enhance the osseointegration process. Several methods of surface modification are chronologically described bridging ordinary methods (e.g. grit blasting and etching) and advanc...
journal_title：Acta biomaterialia
pub_type： 杂志文章,评审
doi：10.1016/j.actbio.2019.05.045
更新日期：2019-08-01 00:00:00
abstract：:The objective of our study was to evaluate the influence of prolonged artificial ageing on oxidation resistance and the subsequent wear behaviour of vitamin E-stabilized, in comparison to standard and highly cross-linked remelted polyethylene (XLPE), and the degradation effect of third-body particles on highly cross-l...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.02.052
更新日期：2014-07-01 00:00:00
abstract：:In this paper, we report X-ray diffraction investigations as well as Raman and solid-state (31)P and (23)Na magic angle spinning nuclear magnetic resonance (NMR) characterization of three series of calcium orthophosphates. The general formulae of the studied compounds are Ca(10.5-x/2)M(x)(PO(4))(7), where M=K or Na an...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.12.016
更新日期：2011-04-01 00:00:00
abstract：:This study assessed the role of immobilized cell adhesion moieties on controlling the cellular attachment, adhesion and phenotype of cardiac muscle cells towards developing scaffolds for cardiac muscle tissue engineering. Collagen I, laminin and the cell-adhesive oligopeptide, arginine-glycine-aspartic acid (RGD) at c...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.05.011
更新日期：2009-10-01 00:00:00
abstract：:This study deals with cytotoxicity assays performed on an array of commercially manufactured inorganic nanoparticulate materials, including Ag, TiO(2), Fe(2)O(3), Al(2)O(3), ZrO(2), Si(3)N(4), naturally occurring mineral chrysotile asbestos and carbonaceous nanoparticulate materials such as multiwall carbon nanotube a...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.11.004
更新日期：2007-05-01 00:00:00
abstract：:When a balloon-angioplasty is performed, the arterial wall is overstretched and thereby damaged, which leads to a stiffness reduction in the arterial layers. An anisotropic damage model able to reflect the main damage mechanisms in overstretched arterial walls is used in combination with a polyconvex hyperelastic stor...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2006.06.005
更新日期：2006-11-01 00:00:00
abstract：UNLABELLED:Medical device-associated infections are a multi-billion dollar burden for the worldwide healthcare systems. The modification of medical devices with non-leaching coatings capable of killing microorganisms on contact is one of the strategies being investigated to prevent microorganism colonization. Here we d...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.01.035
更新日期：2016-03-01 00:00:00
abstract：UNLABELLED:Natural amino acids are zwitterionic molecules and the good biocompatibility promises them potential candidates as anti-fouling materials. Here, we developed a new method to functionalize silica nanoparticles with a natural amino acid-based anti-fouling layer. Amino acids were covalently immobilized on 3-ami...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.03.035
更新日期：2016-08-01 00:00:00
abstract：:Molecular imprinting is a new and rapidly evolving technique used to create synthetic receptors and it possesses great potential in a number of applications in the life sciences. Keeping in mind the therapeutic importance of 5-fluorouracil (5-FU) and the technological significance of molecular imprinting polymers, the...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2008.03.017
更新日期：2008-09-01 00:00:00
abstract：UNLABELLED:Type I collagen is widely applied as a biomaterial for tissue regeneration. In the extracellular matrix, collagen provides strength but not elasticity under large deformations, a characteristic crucial for dynamic organs and generally imparted by elastic fibers. In this study, a methodology is described to i...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.08.038
更新日期：2016-10-15 00:00:00
abstract：:Highly porous titanium with aligned large pores up to 500 μm in size, which is suitable for scaffold applications, was successfully fabricated using the reverse freeze casting method. In this process we have newly developed, the Ti powders migrated spontaneously along the pre-aligned camphene boundaries at a temperatu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2012.03.020
更新日期：2012-07-01 00:00:00
abstract：:Hydroxyapatite (HA) is the main inorganic component of human bone. Inspired by nacre and cortical bone, hydroxyapatite-based coil scaffolds were successfully prepared. The scaffolds presented "brick and mortar" multi-layered structure of nacre and multi-layered concentric circular structure of cortical bone. Because o...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.11.010
更新日期：2021-01-01 00:00:00
abstract：:After skin tissue injury or pathological removal, vascularization timing is paramount in graft survival. As full thickness skin grafts often fail to become perfused over larger surfaces, split-thickness grafts are preferred and can be used together with biomaterials, which themselves are non-angiogenic. One way of pro...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.01.050
更新日期：2020-04-01 00:00:00
abstract：:Postoperative adhesion is a serious complication that can further lead to morbidity and/or mortality. Polymer anti-adhesion barrier material provides an effective precaution to reduce the probability of postoperative adhesion. Clinical application requires these materials to be easily handled, biocompatible, biodegrad...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2017.08.047
更新日期：2017-10-15 00:00:00
abstract：:A predictive framework for the evolution of stem cell biology in 3-D is currently lacking. In this study we propose deep image informatics of the nuclear biology of stem cells to elucidate how 3-D biomaterials steer stem cell lineage phenotypes. The approach is based on high content imaging informatics to capture minu...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2016.08.052
更新日期：2016-11-01 00:00:00
abstract：:The Mg-Ca alloy system has been proposed as a potential new kind of degradable biomaterial with possible application within bone. Here microarc oxidation (MAO) coatings were fabricated on top of a Mg-Ca alloy using different applied voltages and the effect of applied voltage on the surface morphology and phase constit...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.11.034
更新日期：2011-04-01 00:00:00
abstract：:Calcium hydroxide (Ca(OH)(2)) has been broadly used in endodontics, including apexification to obtain apical closure by mineralization. However, the detailed mechanism of mineralization induced by Ca(OH)(2) is still unclear. This study focuses on the function of calcium and hydroxyl ions which dissociate from Ca(OH)(2...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.08.005
更新日期：2010-02-01 00:00:00
abstract：:Nanocrystalline calcium phosphates containing carbonate have a high similarity to bone mineral. The reactions of bone cells (primary osteoblasts and osteoclast-like cells) on these materials as well as on sintered beta-tricalcium phosphate and hydroxyapatite (HA) confirmed a good biocompatibility of the nanocrystallin...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.03.003
更新日期：2010-08-01 00:00:00
abstract：:Cell therapy is a promising strategy to treat ischemic diseases, but the efficacy is limited due to high rate of cell death under low oxygen environment of the ischemic tissues. Sustained release of oxygen to continuously oxygenate the transplanted cells may augment cell survival and improve therapeutic efficacy. We h...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2020.08.031
更新日期：2020-10-01 00:00:00
abstract：:Understanding the influence of a controlled spatial distribution of biological cues on cell activities can be useful to design "cell instructive" materials, able to control and guide the formation of engineered tissues in vivo and in vitro. To this purpose, biochemical and mechanical properties of the resulting biomat...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2009.12.050
更新日期：2010-07-01 00:00:00
abstract：:Hydrogels can provide a suitable environment for tissue formation by embedded cells, which makes them suitable for applications in regenerative medicine. However, hydrogels possess only limited mechanical strength, and must therefore be reinforced for applications in load-bearing conditions. In most approaches the rei...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2014.02.041
更新日期：2014-06-01 00:00:00
abstract：:Despite their known osteoconductivity, clinical use of calcium phosphate cements is limited both by their relatively slow rate of resorption and by rheological properties incompatible with injectability. Bone in-growth and material resorption have been improved by the development of porous calcium phosphate cements. H...
journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.12.012
更新日期：2011-04-01 00:00:00
