High-density antimicrobial peptide coating with broad activity and low cytotoxicity against human cells.

Abstract:

UNLABELLED:Medical device-associated infections are a multi-billion dollar burden for the worldwide healthcare systems. The modification of medical devices with non-leaching coatings capable of killing microorganisms on contact is one of the strategies being investigated to prevent microorganism colonization. Here we developed a robust antimicrobial coating based on the chemical immobilization of the antimicrobial peptide (AMP), cecropin-melittin (CM), on gold nanoparticles coated surfaces. The concentration of AMP immobilized (110 μg/cm(2)) was higher than most of the studies reported so far (<10 μg/cm(2)). This translated onto a coating with high antimicrobial activity against Gram positive and negative bacteria sp., as well as multi-drug resistant bacteria. Studies with E. coli reporter bacteria showed that these coatings induced the permeability of the outer membrane of bacteria in less than 5 min and the inner membrane in approximately 20 min. Importantly, the antimicrobial properties of the coating are maintained in the presence of 20% (v/v) human serum, and have low probability to induce bacteria resistance. We further show that coatings have low toxicity against human endothelial and fibroblast cells and is hemocompatible since it does not induce platelet and complement activation. The antimicrobial coating described here may be promising to prevent medical device-associated infections. STATEMENT OF SIGNIFICANCE:In recent years, antimicrobial peptides (AMPs) have been chemically immobilized on surfaces of medical devices to render them with antimicrobial properties. Surfaces having immobilized cationic peptides are susceptible to be adsorbed by plasma proteins with the subsequent loss of antimicrobial activity. Furthermore, with the exception of very few studies that have determined the cytotoxicity of surfaces in mammalian cells, the effect of the immobilized AMP on human cells is relatively unknown. Here we report a coating based on cecropin-melittin peptide (CM) that maintains its antimicrobial activity against Gram-positive and negative bacteria including multi-drugs resistance bacteria in the presence of serum and has relatively low cytotoxicity against human cells. The reported coatings may be translated on to variety of substrates (glass and titanium) and medical devices to prevent device-associated microbial infection.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Rai A,Pinto S,Evangelista MB,Gil H,Kallip S,Ferreira MG,Ferreira L

doi

10.1016/j.actbio.2016.01.035

subject

Has Abstract

pub_date

2016-03-01 00:00:00

pages

64-77

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(16)30035-6

journal_volume

33

pub_type

杂志文章
  • Covalently crosslinked chitosan hydrogel: properties of in vitro degradation and chondrocyte encapsulation.

    abstract::In vitro degradation and chondrocyte-encapsulation of chitosan hydrogel made of crosslinkable and water-soluble chitosan derivative (CML) at neutral pH and body temperature were studied with respect to weight loss, cytoviability, DNA content and cell morphology. In vitro degradation of the chitosan hydrogels was sensi...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2006.06.007

    authors: Hong Y,Song H,Gong Y,Mao Z,Gao C,Shen J

    更新日期:2007-01-01 00:00:00

  • Modular polymer design to regulate phenotype and oxidative response of human coronary artery cells for potential stent coating applications.

    abstract::Polymer properties can be tailored by copolymerizing subunits with specific physico-chemical characteristics. Vascular stent materials require biocompatibility, mechanical strength, and prevention of restenosis. Here we copolymerized poly(ε-caprolactone) (PCL), poly(ethylene glycol) (PEG), and carboxyl-PCL (cPCL) at v...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.10.003

    authors: Crowder SW,Gupta MK,Hofmeister LH,Zachman AL,Sung HJ

    更新日期:2012-02-01 00:00:00

  • A natural functionally graded biocomposite coating--human enamel.

    abstract::Human enamel has been found to be a coating with excellent mechanical performance, and has undergone extensive investigation and discussion. However, most of the reported studies consider the enamel as a homogeneous anisotropic biocomposite. The current study illustrated the graded properties of the biocomposite from ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.12.029

    authors: He LH,Yin ZH,van Vuuren LJ,Carter EA,Liang XW

    更新日期:2013-05-01 00:00:00

  • Functional biopolymer-based matrices for modulation of chronic wound enzyme activities.

    abstract::Collagen, collagen/hyaluronic acid (HA) and collagen/HA/chitosan (CS) sponges loaded with epigallocatechin gallate (EGCG), catechin (CAT) and gallic acid (GA) were developed and evaluated as active chronic wound dressings. Their physico-mechanical properties, biostability, biocompatibility and ability to inhibit in vi...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.10.014

    authors: Francesko A,Soares da Costa D,Reis RL,Pashkuleva I,Tzanov T

    更新日期:2013-02-01 00:00:00

  • Influence of cyclic fatigue in water on the load-bearing capacity of dental bridges made of zirconia.

    abstract::The humid atmosphere and permanent occurrence of chewing forces in the oral environment lead to degradation of ceramics used for prosthetic restorations. The aim of this in vitro study was to evaluate the influence of artificial aging on the load-bearing capacity of four-unit bridges, with both undamaged and predamage...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2008.04.012

    authors: Kohorst P,Dittmer MP,Borchers L,Stiesch-Scholz M

    更新日期:2008-09-01 00:00:00

  • In vitro and in vivo assessment of controlled release and degradation of acoustically responsive scaffolds.

    abstract::Spatiotemporally controlled release of growth factors (GFs) is critical for regenerative processes such as angiogenesis. A common strategy is to encapsulate the GF within hydrogels, with release being controlled via diffusion and/or gel degradation (i.e., hydrolysis and/or proteolysis). However, simple encapsulation s...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.09.026

    authors: Moncion A,Arlotta KJ,O'Neill EG,Lin M,Mohr LA,Franceschi RT,Kripfgans OD,Putnam AJ,Fabiilli ML

    更新日期:2016-12-01 00:00:00

  • Engineered dermal equivalent tissue in vitro by assembly of microtissue precursors.

    abstract::Tissue-engineered constructs can be fabricated by the assembly of smaller building blocks in order to mimic much of the native biology that is often made from repeating functional units. Our aim was to realize a three-dimensional (3-D) tissue-like construct in vitro by inducing the assembly of functional micrometric t...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.01.026

    authors: Palmiero C,Imparato G,Urciuolo F,Netti P

    更新日期:2010-07-01 00:00:00

  • Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells.

    abstract::Numerous studies have shown that the physicochemical properties of biomaterials can control cell activity. Cell adhesion, proliferation, differentiation as well as tissue formation in vivo can be tuned by properties such as the porosity, surface micro- and nanoscale topography and chemical composition of biomaterials....

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.04.021

    authors: Barradas AM,Lachmann K,Hlawacek G,Frielink C,Truckenmoller R,Boerman OC,van Gastel R,Garritsen H,Thomas M,Moroni L,van Blitterswijk C,de Boer J

    更新日期:2012-08-01 00:00:00

  • Antibiofilm elastin-like polypeptide coatings: functionality, stability, and selectivity.

    abstract::Antimicrobial peptides (AMPs) are currently receiving interest as an alternative to conventional antibiotics to treat biomaterial-associated infection. However, the inherent instability of such peptides often limits their efficacy in intended clinical applications. Covalent immobilization of AMPs to surfaces is one st...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.10.039

    authors: Atefyekta S,Pihl M,Lindsay C,Heilshorn SC,Andersson M

    更新日期:2019-01-01 00:00:00

  • Improved bone-forming functionality on diameter-controlled TiO(2) nanotube surface.

    abstract::The titanium dioxide (TiO(2)) nanotube surface enables significantly accelerated osteoblast adhesion and exhibits strong bonding with bone. We prepared various sizes (30-100 nm diameter) of titanium dioxide (TiO(2)) nanotubes on titanium substrates by anodization and investigated the osteoblast cellular behavior in re...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.05.008

    authors: Brammer KS,Oh S,Cobb CJ,Bjursten LM,van der Heyde H,Jin S

    更新日期:2009-10-01 00:00:00

  • Nanoengineered injectable hydrogels for wound healing application.

    abstract::We report injectable nanoengineered hemostats for enhanced wound healing and tissue regeneration. The nanoengineered system consists of the natural polysaccharide, κ-carrageenan (κCA), loaded with synthetic two-dimensional (2D) nanosilicates. Nanoengineered hydrogels showed shear-thinning characteristics and can be in...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.01.045

    authors: Lokhande G,Carrow JK,Thakur T,Xavier JR,Parani M,Bayless KJ,Gaharwar AK

    更新日期:2018-04-01 00:00:00

  • Screening of rat mesenchymal stem cell behaviour on polydimethylsiloxane stiffness gradients.

    abstract::Substrate stiffness is emerging as an effective tool for the regulation of cell behaviours such as locomotion, proliferation and differentiation. In order to explore the potential application of this biophysical tool, material platforms displaying lateral and continuously graded stiffness are advantageous since they a...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.09.030

    authors: Wang PY,Tsai WB,Voelcker NH

    更新日期:2012-02-01 00:00:00

  • Development of bioactive and biodegradable chitosan-based injectable systems containing bioactive glass nanoparticles.

    abstract::There is increasing interest in the development of new tissue engineering strategies to deliver cells and bioactive agents encapsulated in a biodegradable matrix through minimally invasive procedures. The present work proposes to combine chitosan-beta-glycerophosphate salt formulations with bioactive glass nanoparticl...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2008.08.006

    authors: Couto DS,Hong Z,Mano JF

    更新日期:2009-01-01 00:00:00

  • Triple co-culture of human alveolar epithelium, endothelium and macrophages for studying the interaction of nanocarriers with the air-blood barrier.

    abstract::Predictive in vitro models are valuable alternatives to animal experiments for evaluating the transport of molecules and (nano)particles across biological barriers. In this work, an improved triple co-culture of air-blood barrier was set-up, being exclusively constituted by human cell lines that allowed to perform exp...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.04.037

    authors: Costa A,de Souza Carvalho-Wodarz C,Seabra V,Sarmento B,Lehr CM

    更新日期:2019-06-01 00:00:00

  • Repair of segmental ulna defects using a β-TCP implant in combination with a heparan sulfate glycosaminoglycan variant.

    abstract:UNLABELLED:Given the wide spread clinical use of ceramic-based bone void fillers, we sought to determine the efficacy of an FDA-approved β-tricalcium phosphate bone graft substitute (JAX™) in combination with a carboxymethyl cellulose (CMC) handling agent that included a particular heparan glycosaminoglycan (GAG) varia...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.09.008

    authors: Rai B,Chatterjea A,Lim ZXH,Tan TC,Sawyer AA,Hosaka YZ,Murali S,Lee JJL,Fenwick SA,Hui JH,Nurcombe V,Cool SM

    更新日期:2015-12-01 00:00:00

  • A collagen hydrogel loaded with HDAC7-derived peptide promotes the regeneration of infarcted myocardium with functional improvement in a rodent model.

    abstract::Myocardial infarction (MI) leads to the loss of cardiomyocytes, left ventricle (LV) dilation, and cardiac dysfunction, eventually developing into heart failure. Most of the strategies for MI therapy require biomaterials that can support tissue regeneration. In this study, we hypothesized that the extracellular matrix ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.01.022

    authors: Zhang Y,Zhu D,Wei Y,Wu Y,Cui W,Liuqin L,Fan G,Yang Q,Wang Z,Xu Z,Kong D,Zeng L,Zhao Q

    更新日期:2019-03-01 00:00:00

  • Enzymatic conjugation of a bioactive peptide into an injectable hyaluronic acid-tyramine hydrogel system to promote the formation of functional vasculature.

    abstract::In this study, one-step enzyme-mediated preparation of a multi-functional injectable hyaluronic-acid-based hydrogel system is reported. Hydrogel was formed through the in situ coupling of phenol moieties by horseradish peroxidase (HRP) and hydrogen peroxide (H2O2), and bioactive peptides were simultaneously conjugated...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.02.022

    authors: Wang LS,Lee F,Lim J,Du C,Wan AC,Lee SS,Kurisawa M

    更新日期:2014-06-01 00:00:00

  • Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization.

    abstract::Decades of research have been undertaken towards the goal of tissue engineering using xenogeneic scaffolds. The primary advantages associated with use of xenogeneic tissue-derived scaffolds for in vitro development of replacement tissues and organs stem from the inherent extracellular matrix (ECM) composition and arch...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章,评审

    doi:10.1016/j.actbio.2014.01.028

    authors: Wong ML,Griffiths LG

    更新日期:2014-05-01 00:00:00

  • Adhesion, migration and mechanics of human adipose-tissue-derived stem cells on silk fibroin-chitosan matrix.

    abstract::Silk fibroin-chitosan (SFCS) scaffold is a naturally derived biocompatible matrix with potential reconstructive surgical applications. In this study, human adipose-derived mesenchymal stem cells (ASCs) were seeded on SFCS scaffolds and cell attachment was characterized by fluorescence, confocal, time-lapse, atomic for...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.10.034

    authors: Altman AM,Gupta V,Ríos CN,Alt EU,Mathur AB

    更新日期:2010-04-01 00:00:00

  • Retinal ganglion cell polarization using immobilized guidance cues on a tissue-engineered scaffold.

    abstract::Cell transplantation therapies to treat diseases related to dysfunction of retinal ganglion cells (RGCs) are limited in part by an inability to navigate to the optic nerve head within the retina. During development, RGCs are guided by a series of neurotrophic factors and guidance cues; however, these factors and their...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.08.032

    authors: Kador KE,Alsehli HS,Zindell AN,Lau LW,Andreopoulos FM,Watson BD,Goldberg JL

    更新日期:2014-12-01 00:00:00

  • Electrospun fibrous scaffolds with continuous gradations in mineral contents and biological cues for manipulating cellular behaviors.

    abstract::Challenges remain in the generation of heterogeneous tissues and the repairing of interfacial tissue between soft and hard tissues. The development of tissue engineering scaffolds with gradients in composition, structure, mechanical and chemical properties is essential to modulate cellular behaviors in a graded way an...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.01.003

    authors: Zou B,Liu Y,Luo X,Chen F,Guo X,Li X

    更新日期:2012-04-01 00:00:00

  • Heterogeneous role of integrins in fibroblast response to small cyclic mechanical stimulus generated by a nanoporous gold actuator.

    abstract::It is important to understand the effects of mechanical stimulation on cell behaviors for homeostasis. Many studies have been performed on cell responses to mechanical stimuli, but the mechanosensing mechanism is still under debate. In the present study, experiments employing molecular dynamics (MD) simulations concer...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.12.014

    authors: Deguchi S,Kato A,Wu P,Hakamada M,Mabuchi M

    更新日期:2021-02-01 00:00:00

  • Three-dimensional arrangement of β-tricalcium phosphate granules evaluated by microcomputed tomography and fractal analysis.

    abstract::The macrophysical properties of granular biomaterials used to fill bone defects have rarely been considered. Granules of a given biomaterial occupy three-dimensional (3-D) space when packed together and create a macroporosity suitable for the invasion of vascular and bone cells. Granules of β-tricalcium phosphate were...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.09.015

    authors: Ndiaye M,Terranova L,Mallet R,Mabilleau G,Chappard D

    更新日期:2015-01-01 00:00:00

  • Improved burn wound healing by the antimicrobial peptide LLKKK18 released from conjugates with dextrin embedded in a carbopol gel.

    abstract:UNLABELLED:Antimicrobial peptides (AMPs) are good candidates to treat burn wounds, a major cause of morbidity, impaired life quality and resources consumption in developed countries. We took advantage of a commercially available hydrogel, Carbopol®, a vehicle for topical administration that maintains a moist environmen...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.07.043

    authors: Silva JP,Dhall S,Garcia M,Chan A,Costa C,Gama M,Martins-Green M

    更新日期:2015-10-01 00:00:00

  • Amino acid-based anti-fouling functionalization of silica nanoparticles using divinyl sulfone.

    abstract:UNLABELLED:Natural amino acids are zwitterionic molecules and the good biocompatibility promises them potential candidates as anti-fouling materials. Here, we developed a new method to functionalize silica nanoparticles with a natural amino acid-based anti-fouling layer. Amino acids were covalently immobilized on 3-ami...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.03.035

    authors: Wang H,Cheng F,Shen W,Cheng G,Zhao J,Peng W,Qu J

    更新日期:2016-08-01 00:00:00

  • Fibronectin fiber creep under constant force loading.

    abstract::Viscoelasticity is a fundamental property of virtually all biological materials, and proteinaceous, fibrous materials that constitute the extracellular matrix (ECM) are no exception. Viscoelasticity may be particularly important in the ECM since cells can apply mechanical stress resulting from cell contractility over ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.02.022

    authors: Bradshaw MJ,Hoffmann GA,Wong JY,Smith ML

    更新日期:2019-04-01 00:00:00

  • Effects of neighboring sulfides and pH on ester hydrolysis in thiol-acrylate photopolymers.

    abstract::Networks synthesized through thiol-acrylate photopolymerization or Michael-type addition step growth reactions contain esters with neighboring sulfide groups. Previous work has demonstrated that these esters are readily hydrolyzable at physiological pH. Here, the influence of the distance between the sulfide and ester...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2006.12.001

    authors: Rydholm AE,Anseth KS,Bowman CN

    更新日期:2007-07-01 00:00:00

  • Preliminary evaluation of molecular imprinting of 5-fluorouracil within hydrogels for use as drug delivery systems.

    abstract::Molecular imprinting is a new and rapidly evolving technique used to create synthetic receptors and it possesses great potential in a number of applications in the life sciences. Keeping in mind the therapeutic importance of 5-fluorouracil (5-FU) and the technological significance of molecular imprinting polymers, the...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2008.03.017

    authors: Singh B,Chauhan N

    更新日期:2008-09-01 00:00:00

  • Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient.

    abstract::Sickle cell disease (SCD) is characterized by the abnormal deformation of red blood cells (RBCs) in the deoxygenated condition, as their elongated shape leads to compromised circulation. The pathophysiology of SCD is influenced by both the biomechanical properties of RBCs and their hemodynamic properties in the microv...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.07.011

    authors: Byun H,Hillman TR,Higgins JM,Diez-Silva M,Peng Z,Dao M,Dasari RR,Suresh S,Park Y

    更新日期:2012-11-01 00:00:00

  • The in vivo anti-fibrotic function of calcium sensitive receptor (CaSR) modulating poly(p-dioxanone-co-l-phenylalanine) prodrug.

    abstract::In present study, the apoptosis induction and proliferation suppression effects of l-phenylalanine (l-Phe) on fibroblasts were confirmed. The action sites of l-Phe on fibroblasts suppression were deduced to be calcium sensitive receptor (CaSR) which could cause the release of endoplasmic reticulum (ER) Ca2+ stores; di...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.04.018

    authors: Wang B,Wen A,Feng C,Niu L,Xiao X,Luo L,Shen C,Zhu J,Lei J,Zhang X

    更新日期:2018-06-01 00:00:00