当前位置： SCI文献检索 > Acta Biomaterialia期刊下所有文献 > Lipoconstruct surface topography grating size influences vascularization onset in the dorsal skinfold chamber model.

Lipoconstruct surface topography grating size influences vascularization onset in the dorsal skinfold chamber model.

Abstract：

:After skin tissue injury or pathological removal, vascularization timing is paramount in graft survival. As full thickness skin grafts often fail to become perfused over larger surfaces, split-thickness grafts are preferred and can be used together with biomaterials, which themselves are non-angiogenic. One way of promoting vascular ingrowth is to "pre-vascularize" an engineered substitute by introducing endothelial cells (ECs). Since it has been previously demonstrated that surface structured biomaterials have an effect on wound healing, skin regeneration, and fibrosis reduction, we proposed that a microvascular-rich lipoconstruct with anisotropic topographical cues could be a clinically translatable vascularization approach. Murine lipofragments were formed with three polydimethylsiloxane molds (flat, 5 µm, and 50 µm parallel gratings) and implanted into the dorsal skinfold chamber of male C57BL/6 mice. Vascular ingrowth was observed through intravital microscopy over 21 days and further assessed by histology and protein identification. Our investigation revealed that topographical feature size influenced the commencement of neovascular ingrowth, with 5 µm gratings exhibiting early construct perfusion at 3 days post-operation, and 50 µm being delayed until day 5. We therefore postulate that surface structured lipoconstructs may serve as an easily obtained and produced construct suitable for providing soft tissue and ECs to tissue defects. STATEMENT OF SIGNIFICANCE: Skin graft failures due to inadequate or uneven perfusion frequently occur and can be even more complicated in deep, difficult to heal wounds, or bone coverage. In complex injuries, biomaterials are often used to cover bone structures with a standard split thickness graft; however, perfusion can take up to 3 weeks. Thus, any means to promote faster and uniform vascularization could significantly reduce healing time, as well as lower patient down-time. As pre-vascularized constructs have reported success in research, we created a cost-efficient, translatable method with no additional laboratory time as adipose tissue can be harvested and used immediately. We further used surface topography as an aspect to modulate construct perfusion, which has been reported for the first time here.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

McLuckie M,Robotti F,Sanchez-Macedo N,Enderlin D,Frese L,Cheng PF,Levesque MP,Egaña JT,Poulikakos D,Ferrari A,Lindenblatt N

doi

10.1016/j.actbio.2020.01.050

subject

Has Abstract

pub_date

2020-04-01 00:00:00

pages

136-144

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(20)30068-4

journal_volume

106

pub_type

杂志文章

在线工具

Lipoconstruct surface topography grating size influences vascularization onset in the dorsal skinfold chamber model.