Fibrin-based 3D matrices induce angiogenic behavior of adipose-derived stem cells.

Abstract:

:Engineered three-dimensional biomaterials are known to affect the regenerative capacity of stem cells. The extent to which these materials can modify cellular activities is still poorly understood, particularly for adipose-derived stem cells (ASCs). This study evaluates PEGylated fibrin (P-fibrin) gels as an ASC-carrying scaffold for encouraging local angiogenesis by comparing with two commonly used hydrogels (i.e., collagen and fibrin) in the tissue-engineering field. Human ASCs in P-fibrin were compared to cultures in collagen and fibrin under basic growth media without any additional soluble factors. ASCs proliferated similarly in all gel scaffolds but showed significantly elongated morphologies in the P-fibrin gels relative to other gels. P-fibrin elicited higher von Willebrand factor expression in ASCs than either collagen or fibrin while cells in collagen expressed more smooth muscle alpha actin than in other gels. VEGF was secreted more at 7 days in fibrin and P-fibrin than in collagen and several other angiogenic and immunomodulatory cytokines were similarly enhanced. Fibrin-based matrices appear to activate angiogenic signaling in ASCs while P-fibrin matrices are uniquely able to also drive a vessel-like ASC phenotype. Collectively, these results suggest that P-fibrin promotes the angiogenic potential of ASC-based therapeutic applications.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Chung E,Rytlewski JA,Merchant AG,Dhada KS,Lewis EW,Suggs LJ

doi

10.1016/j.actbio.2015.01.012

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

78-88

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(15)00013-6

journal_volume

17

pub_type

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