Abstract:
:Understanding the influence of a controlled spatial distribution of biological cues on cell activities can be useful to design "cell instructive" materials, able to control and guide the formation of engineered tissues in vivo and in vitro. To this purpose, biochemical and mechanical properties of the resulting biomaterial must be carefully designed and controlled. In this work, the effect of covalently immobilized RGD peptide gradients on poly(ethylene glycol) diacrylate hydrogels on cell behaviour was studied. We set up a mechanical device generating gradients based on a fluidic chamber. Cell response to RGD gradients with different slope (0.7, 1 and 2 mM cm(-1)) was qualitatively and quantitatively assessed by evaluating cell adhesion and, in particular, cell migration, compared to cells seeded on hydrogels with uniform distribution of RGD peptides. To evaluate the influence of RGD gradient and to exclude any concentration effect on cell response, all analyses were carried out in a specific region of the gradients which displayed the same average concentration of RGD (1.5 mM). Results suggest that cells recognize the RGD gradient and adhere onto it assuming a stretched shape. Moreover, cells tend to migrate in the direction of the gradient, as their speed is higher than that of cells migrating on hydrogels with a uniform distribution of RGD and increases by increasing RGD gradient steepness. This increment is due to an augmentation of bias speed component of the mean squared speed, that is, the drift of the cell population migrating on the anisotropic surface provided by the RGD gradient.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Guarnieri D,De Capua A,Ventre M,Borzacchiello A,Pedone C,Marasco D,Ruvo M,Netti PAdoi
10.1016/j.actbio.2009.12.050subject
Has Abstractpub_date
2010-07-01 00:00:00pages
2532-9issue
7eissn
1742-7061issn
1878-7568pii
S1742-7061(09)00591-1journal_volume
6pub_type
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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更新日期:2020-10-01 00:00:00
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2007.08.013
更新日期:2008-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2012-08-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2007.05.010
更新日期:2007-11-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2013.09.017
更新日期:2014-01-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2010.12.031
更新日期:2011-05-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2011.06.006
更新日期:2011-10-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2009.10.002
更新日期:2010-04-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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journal_title:Acta biomaterialia
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更新日期:2016-12-01 00:00:00
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更新日期:2020-09-15 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2019.01.022
更新日期:2019-03-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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