Abstract:
:Several studies have reported the benefits of mesenchymal stem cells (MSCs) for bone tissue engineering. However, vascularization remains one of the main obstacles that must be overcome to reconstruct large bone defects. In vitro prevascularization of the three-dimensional (3-D) constructs using co-cultures of human progenitor-derived endothelial cells (PDECs) with human bone marrow mesenchymal stem cells (HBMSCs) appeared as a potential strategy. However, the crosstalk between the two lineages has been studied in two-dimensional (2-D), but remains unknown in 3-D. The aim of this study is to investigate the cell interactions between PDECs and HBMSCs in a porous matrix composed of polysaccharides. This biodegradable scaffold promotes cell interactions by inducing multicellular aggregates composed of HBMSCs surrounded by PDECs. Cell aggregation contributes to the formation of junctional proteins composed of Connexin43 (Cx43) and VE-cadherin, and an activation of osteoblastic differentiation of HBMSCs stimulated by the presence of PDECs. Inhibition of Cx43 by mimetic peptide 43GAP27 induced a decrease in mRNA levels of Cx43 and all the bone-specific markers. Finally, subcutaneous implantations for 3 and 8 weeks in NOG mice revealed an increase in osteoid formation with the tissue-engineered constructs seeded with HBMSCs/PDECs compared with those loaded with HBMSCs alone. Taking together, these results demonstrate that this 3-D microenvironment favored cell communication, osteogenesis and bone formation.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Guerrero J,Catros S,Derkaoui SM,Lalande C,Siadous R,Bareille R,Thébaud N,Bordenave L,Chassande O,Le Visage C,Letourneur D,Amédée Jdoi
10.1016/j.actbio.2013.05.025subject
Has Abstractpub_date
2013-09-01 00:00:00pages
8200-13issue
9eissn
1742-7061issn
1878-7568pii
S1742-7061(13)00274-2journal_volume
9pub_type
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journal_title:Acta biomaterialia
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更新日期:2018-05-01 00:00:00
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journal_title:Acta biomaterialia
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更新日期:2014-05-01 00:00:00
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更新日期:2021-02-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2006.07.009
更新日期:2007-05-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2015.07.028
更新日期:2015-10-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
doi:10.1016/j.actbio.2012.12.004
更新日期:2013-04-01 00:00:00
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章,评审
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更新日期:2016-08-01 00:00:00
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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更新日期:2010-01-01 00:00:00
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章,评审
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abstract:UNLABELLED:Antimicrobial peptides (AMPs) are good candidates to treat burn wounds, a major cause of morbidity, impaired life quality and resources consumption in developed countries. We took advantage of a commercially available hydrogel, Carbopol®, a vehicle for topical administration that maintains a moist environmen...
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