A postoperative anti-adhesion barrier based on photoinduced imine-crosslinking hydrogel with tissue-adhesive ability.

Abstract:

:Postoperative adhesion is a serious complication that can further lead to morbidity and/or mortality. Polymer anti-adhesion barrier material provides an effective precaution to reduce the probability of postoperative adhesion. Clinical application requires these materials to be easily handled, biocompatible, biodegradable, and most importantly tissue adherent to provide target sites with reliable isolation. However, currently there is nearly no polymer barrier material that can fully satisfy these requirements. In this study, based on the photoinduced imine-crosslinking (PIC) reaction, we had developed a photo-crosslinking hydrogel (CNG hydrogel) that composed of o-nitrobenzyl alcohol (NB) modified carboxymethyl cellulose (CMC-NB) and glycol chitosan (GC) as an anti-adhesion barrier material. Under light irradiation, CMC-NB generated aldehyde groups which subsequently reacted with amino groups distributed on GC or tissue surface to form a hydrogel barrier that covalently attached to tissue surface. Rheological analysis demonstrated that CNG hydrogel (30mg/mL polymer content) could be formed in 30s upon light irradiation. Tissue adhesive tests showed that the tissue adhesive strength of CNG hydrogel (30mg/mL) was about 8.32kPa-24.65kPa which increased with increasing CMC-NB content in CNG hydrogel. Toxicity evaluation by L929 cells demonstrated that CNG hydrogel was cytocompatible. Furthermore, sidewall defect-cecum abrasion model of rat was employed to evaluate the postoperative anti-adhesion efficacy of CNG hydrogel. And a significantly reduction of tissue adhesion (20% samples with low score adhesion) was found in CNG hydrogel treated group, compared with control group (100% samples with high score adhesion). In addition, CNG hydrogel could be degraded in nearly 14days and showed no side effect on wound healing. These findings indicated that CNG hydrogel can effectively expanded the clinical treatments of postoperative tissue adhesion. STATEMENT OF SIGNIFICANCE:In this study, a tissue adhesive photo-crosslinking hydrogel (CNG) was developed based on photo-induced imine crosslinking reaction (PIC) for postoperative anti-adhesion. CNG hydrogel showed the features of easy and convenient operation, fast and controllable gelation, suitable gel strength, good biocompatibility, and most importantly strong tissue adhesiveness. Therefore, it shows very high performance to prevent postoperative tissue adhesion. Overall, our study provides a more suitable hydrogel barrier material that can overcome the shortcomings of current barriers for clinical postoperative anti-adhesion.

journal_name

Acta Biomater

journal_title

Acta biomaterialia

authors

Yang Y,Liu X,Li Y,Wang Y,Bao C,Chen Y,Lin Q,Zhu L

doi

10.1016/j.actbio.2017.08.047

subject

Has Abstract

pub_date

2017-10-15 00:00:00

pages

199-209

eissn

1742-7061

issn

1878-7568

pii

S1742-7061(17)30534-2

journal_volume

62

pub_type

杂志文章
  • An interferon-γ-delivery system based on chitosan/poly(γ-glutamic acid) polyelectrolyte complexes modulates macrophage-derived stimulation of cancer cell invasion in vitro.

    abstract::Macrophages represent a large component of the tumour microenvironment and are described to establish interactions with cancer cells, playing crucial roles in several stages of cancer progression. The functional plasticity of macrophages upon stimulation from the environment makes them susceptible to the influence of ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.05.022

    authors: Cardoso AP,Gonçalves RM,Antunes JC,Pinto ML,Pinto AT,Castro F,Monteiro C,Barbosa MA,Oliveira MJ

    更新日期:2015-09-01 00:00:00

  • Effect of hydrogel elasticity and ephrinB2-immobilized manner on Runx2 expression of human mesenchymal stem cells.

    abstract::The objective of this study is to design the manner of ephrinB2 immobilized onto polyacrylamide (PAAm) hydrogels with varied elasticity and evaluate the effect of hydrogels elasticity and the immobilized manner of ephrinB2 on the Runx2 expression of human mesenchymal stem cells (hMSC). The PAAm hydrogels were prepared...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.03.016

    authors: Toda H,Yamamoto M,Uyama H,Tabata Y

    更新日期:2017-08-01 00:00:00

  • Retinal ganglion cell polarization using immobilized guidance cues on a tissue-engineered scaffold.

    abstract::Cell transplantation therapies to treat diseases related to dysfunction of retinal ganglion cells (RGCs) are limited in part by an inability to navigate to the optic nerve head within the retina. During development, RGCs are guided by a series of neurotrophic factors and guidance cues; however, these factors and their...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.08.032

    authors: Kador KE,Alsehli HS,Zindell AN,Lau LW,Andreopoulos FM,Watson BD,Goldberg JL

    更新日期:2014-12-01 00:00:00

  • The synergistic effect of micro-topography and biochemical culture environment to promote angiogenesis and osteogenic differentiation of human mesenchymal stem cells.

    abstract::Critical failures associated with current engineered bone grafts involve insufficient induction of osteogenesis of the implanted cells and lack of vascular integration between graft scaffold and host tissue. This study investigated the combined effects of surface microtextures and biochemical supplements to achieve os...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2015.02.021

    authors: Song S,Kim EJ,Bahney CS,Miclau T,Marcucio R,Roy S

    更新日期:2015-05-01 00:00:00

  • The effect of autoclaving on the physical and biological properties of dicalcium phosphate dihydrate bioceramics: brushite vs. monetite.

    abstract::Dicalcium phosphate dihydrate (brushite) is an osteoconductive biomaterial with great potential as a bioresorbable cement for bone regeneration. Preset brushite cement can be dehydrated into dicalcium phosphate anhydrous (monetite) bioceramics by autoclaving. This heat treatment results in changes in the physical char...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.04.025

    authors: Tamimi F,Le Nihouannen D,Eimar H,Sheikh Z,Komarova S,Barralet J

    更新日期:2012-08-01 00:00:00

  • Cytotoxic effects of aggregated nanomaterials.

    abstract::This study deals with cytotoxicity assays performed on an array of commercially manufactured inorganic nanoparticulate materials, including Ag, TiO(2), Fe(2)O(3), Al(2)O(3), ZrO(2), Si(3)N(4), naturally occurring mineral chrysotile asbestos and carbonaceous nanoparticulate materials such as multiwall carbon nanotube a...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2006.11.004

    authors: Soto K,Garza KM,Murr LE

    更新日期:2007-05-01 00:00:00

  • Biomimetic collagen scaffolds with anisotropic pore architecture.

    abstract::Sponge-like matrices with a specific three-dimensional structural design resembling the actual extracellular matrix of a particular tissue show significant potential for the regeneration and repair of a broad range of damaged anisotropic tissues. The manipulation of the structure of collagen scaffolds using a freeze-d...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.09.033

    authors: Davidenko N,Gibb T,Schuster C,Best SM,Campbell JJ,Watson CJ,Cameron RE

    更新日期:2012-02-01 00:00:00

  • Evaluation of antibiotic releasing porous polymethylmethacrylate space maintainers in an infected composite tissue defect model.

    abstract::This study evaluated the in vitro and in vivo performance of antibiotic-releasing porous polymethylmethacrylate (PMMA)-based space maintainers comprising a gelatin hydrogel porogen and a poly(dl-lactic-co-glycolic acid) (PLGA) particulate carrier for antibiotic delivery. Colistin was released in vitro from either gela...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2013.07.018

    authors: Spicer PP,Shah SR,Henslee AM,Watson BM,Kinard LA,Kretlow JD,Bevil K,Kattchee L,Bennett GN,Demian N,Mende K,Murray CK,Jansen JA,Wong ME,Mikos AG,Kasper FK

    更新日期:2013-11-01 00:00:00

  • Co-culture of human induced pluripotent stem cell-derived retinal pigment epithelial cells and endothelial cells on double collagen-coated honeycomb films.

    abstract::In vitro cell culture models representing the physiological and pathological features of the outer retina are urgently needed. Artificial tissue replacements for patients suffering from degenerative retinal diseases are similarly in great demand. Here, we developed a co-culture system based solely on the use of human ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.11.002

    authors: Calejo MT,Saari J,Vuorenpää H,Vuorimaa-Laukkanen E,Kallio P,Aalto-Setälä K,Miettinen S,Skottman H,Kellomäki M,Juuti-Uusitalo K

    更新日期:2020-01-01 00:00:00

  • Cryogel scaffolds for regionally constrained delivery of lysophosphatidylcholine to central nervous system slice cultures: A model of focal demyelination for multiple sclerosis research.

    abstract::The pathology of multiple sclerosis (MS) is typified by focal demyelinated areas of the brain and spinal cord, which results in axonal degeneration and atrophy. Although the field has made much progress in developing immunomodulatory therapies to reduce the occurrence of these focal lesions, there is a conspicuous lac...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.08.030

    authors: Eigel D,Zoupi L,Sekizar S,Welzel PB,Werner C,Williams A,Newland B

    更新日期:2019-10-01 00:00:00

  • A robust spectroscopic method for the determination of protein conformational composition - Application to the annealing of silk.

    abstract::The physical and mechanical properties of structural proteins such as silk fibroin can be modified by controlled conformational change, which is regularly monitored by Fourier transform infrared spectroscopy by peak fitting of the amide I band envelope. Although many variables affecting peak shape are well established...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.03.058

    authors: Belton DJ,Plowright R,Kaplan DL,Perry CC

    更新日期:2018-06-01 00:00:00

  • An injectable heparin-conjugated hyaluronan scaffold for local delivery of transforming growth factor β1 promotes successful chondrogenesis.

    abstract::Cartilage lacks basic repair mechanisms and thus surgical interventions are necessary to treat lesions. Minimally-invasive arthroscopic procedures require the development of injectable biomaterials to support chondrogenesis of implanted cells. However, most cartilage tissue engineering approaches rely on pre-culture o...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.09.017

    authors: Levinson C,Lee M,Applegate LA,Zenobi-Wong M

    更新日期:2019-11-01 00:00:00

  • Engineered, axially-vascularized osteogenic grafts from human adipose-derived cells to treat avascular necrosis of bone in a rat model.

    abstract:BACKGROUND:Avascular necrosis of bone (AVN) leads to sclerosis and collapse of bone and joints. The standard of care, vascularized bone grafts, is limited by donor site morbidity and restricted availability. The aim of this study was to generate and test engineered, axially vascularized SVF cells-based bone substitutes...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.09.003

    authors: Ismail T,Osinga R,Todorov A Jr,Haumer A,Tchang LA,Epple C,Allafi N,Menzi N,Largo RD,Kaempfen A,Martin I,Schaefer DJ,Scherberich A

    更新日期:2017-11-01 00:00:00

  • Structure and mechanical properties of β-TCP scaffolds prepared by ice-templating with preset ice front velocities.

    abstract::Anisotropic scaffolds with the typical structure of lamellar, aligned and continuous pores were successfully achieved by the directional solidification of water-based β-tricalcium phosphate (β-TCP) suspensions. Adjustable porosities from 49 to 82%, tunable pore widths from 8 to 50μm and linked ceramic cells with wall ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.08.020

    authors: Flauder S,Gbureck U,Müller FA

    更新日期:2014-12-01 00:00:00

  • Biodegradable and adjustable sol-gel glass based hybrid scaffolds from multi-armed oligomeric building blocks.

    abstract::Biodegradability is a crucial characteristic to improve the clinical potential of sol-gel-derived glass materials. To this end, a set of degradable organic/inorganic class II hybrids from a tetraethoxysilane(TEOS)-derived silica sol and oligovalent cross-linker oligomers containing oligo(d,l-lactide) domains was devel...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.09.024

    authors: Kascholke C,Hendrikx S,Flath T,Kuzmenka D,Dörfler HM,Schumann D,Gressenbuch M,Schulze FP,Schulz-Siegmund M,Hacker MC

    更新日期:2017-11-01 00:00:00

  • Mineralized nanofiber segments coupled with calcium-binding BMP-2 peptides for alveolar bone regeneration.

    abstract::Bone loss around tooth extraction sites can occur, thus making future placement of dental implants difficult. Alveolar bone regeneration can be guided by the application of a nanofibrous bone graft coupled with osteoinductive proteins/peptides, following tooth loss or tooth extraction. In the present study, we demonst...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.12.051

    authors: Boda SK,Almoshari Y,Wang H,Wang X,Reinhardt RA,Duan B,Wang D,Xie J

    更新日期:2019-02-01 00:00:00

  • Inkjet printed antibiotic- and calcium-eluting bioresorbable nanocomposite micropatterns for orthopedic implants.

    abstract::Inkjet printing of antibiotic- and calcium-eluting micropatterns was explored as a novel means of preventing the formation of biofilm colonies and facilitating osteogenic cell development on orthopedic implant surfaces. The micropatterns consisted of a periodic array of ∼50 μm circular dots separated by ∼150 μm. The c...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2011.08.006

    authors: Gu Y,Chen X,Lee JH,Monteiro DA,Wang H,Lee WY

    更新日期:2012-01-01 00:00:00

  • Bi-layered micro-fibre reinforced hydrogels for articular cartilage regeneration.

    abstract::Articular cartilage has limited capacity for regeneration and when damaged cannot be repaired with currently available metallic or synthetic implants. We aim to bioengineer a microfibre-reinforced hydrogel that can capture the zonal depth-dependent mechanical properties of native cartilage, and simultaneously support ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2019.06.030

    authors: Castilho M,Mouser V,Chen M,Malda J,Ito K

    更新日期:2019-09-01 00:00:00

  • Antiadhesion effect of the C17 glycerin ester of isoprenoid-type lipid forming a nonlamellar liquid crystal.

    abstract::Postoperative adhesion is a relevant clinical problem that causes a variety of clinical complications after abdominal surgery. The objective of this study is to develop a liquid-type antiadhesion agent and evaluate its efficacy in preventing tissue adhesion in a rat peritoneal adhesion model. The liquid-type agent was...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.12.009

    authors: Murakami T,Hijikuro I,Yamashita K,Tsunoda S,Hirai K,Suzuki T,Sakai Y,Tabata Y

    更新日期:2019-01-15 00:00:00

  • Flt1 peptide-hyaluronate conjugate micelle-like nanoparticles encapsulating genistein for the treatment of ocular neovascularization.

    abstract::Flt1 peptide of GNQWFI is an antagonistic peptide for vascular endothelial growth factor receptor 1 (VEGFR1 or Flt1). In this work, Flt1 peptide-hyaluronate (HA) conjugates were successfully synthesized and the resulting micelle-like nanoparticles were exploited to encapsulate genistein, an inhibitor of tyrosine-speci...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.07.016

    authors: Kim H,Choi JS,Kim KS,Yang JA,Joo CK,Hahn SK

    更新日期:2012-11-01 00:00:00

  • Focal adhesion kinase knockdown modulates the response of human corneal epithelial cells to topographic cues.

    abstract::A rapidly expanding literature broadly documents the impact of biophysical cues on cellular behaviors. In spite of increasing research efforts in this field, the underlying signaling processes are poorly understood. One of the candidate molecules for being involved in mechanotransduction is focal adhesion kinase (FAK)...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.07.004

    authors: Dreier B,Raghunathan VK,Russell P,Murphy CJ

    更新日期:2012-12-01 00:00:00

  • Screening of peptides with a high affinity for ZnO using spot-synthesized peptide arrays and computational analysis.

    abstract::Metal-binding peptides have attracted attention for their usefulness in biomineralization processes. In this work screening of high affinity peptides against ZnO was performed using a combinatorial library approach with a peptide array and computational analysis. The computationally assisted peptide screening and desi...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2009.12.025

    authors: Okochi M,Ogawa M,Kaga C,Sugita T,Tomita Y,Kato R,Honda H

    更新日期:2010-06-01 00:00:00

  • Mesoscale porosity at the dentin-enamel junction could affect the biomechanical properties of teeth.

    abstract::In this paper, the 3D-morphology of the porosity in dentin is investigated within the first 350μm from the dentin-enamel junction (DEJ) by fluorescence confocal laser scanning microscopy (CLSM). We found that the porous microstructure exhibits a much more complex geometry than classically described, which may impact o...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2017.01.052

    authors: Vennat E,Wang W,Genthial R,David B,Dursun E,Gourrier A

    更新日期:2017-03-15 00:00:00

  • Pyrrole-hyaluronic acid conjugates for decreasing cell binding to metals and conducting polymers.

    abstract::Surface modification of electrically conductive biomaterials has been studied to improve biocompatibility for a number of applications, such as implantable sensors and microelectrode arrays. In this study we electrochemically coated electrodes with biocompatible and non-cell adhesive hyaluronic acid (HA) to reduce cel...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2010.06.011

    authors: Lee JY,Schmidt CE

    更新日期:2010-11-01 00:00:00

  • Development of peptide-functionalized synthetic hydrogel microarrays for stem cell and tissue engineering applications.

    abstract::Synthetic polymer microarray technology holds remarkable promise to rapidly identify suitable biomaterials for stem cell and tissue engineering applications. However, most of previous microarrayed synthetic polymers do not possess biological ligands (e.g., peptides) to directly engage cell surface receptors. Here, we ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2016.09.006

    authors: Jia J,Coyle RC,Richards DJ,Berry CL,Barrs RW,Biggs J,James Chou C,Trusk TC,Mei Y

    更新日期:2016-11-01 00:00:00

  • Effects of phosphate glass fiber-collagen scaffolds on functional recovery of completely transected rat spinal cords.

    abstract::Phosphate-based glass fibers (PGFs), due to characteristics such as biodegradability and directionality, could be effective as spatial cues for axonal outgrowth following nerve injury. In the present study, PGF-containing cylindrical scaffolds of 1.8mm diameter and 3mm length were developed and implanted into the gap ...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2012.01.026

    authors: Joo NY,Knowles JC,Lee GS,Kim JW,Kim HW,Son YJ,Hyun JK

    更新日期:2012-05-01 00:00:00

  • Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance.

    abstract::Novel porous bilayered scaffolds, fully integrating a silk fibroin (SF) layer and a silk-nano calcium phosphate (silk-nanoCaP) layer for osteochondral defect (OCD) regeneration, were developed. Homogeneous porosity distribution was achieved in the scaffolds, with calcium phosphate phase only retained in the silk-nanoC...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2014.10.021

    authors: Yan LP,Silva-Correia J,Oliveira MB,Vilela C,Pereira H,Sousa RA,Mano JF,Oliveira AL,Oliveira JM,Reis RL

    更新日期:2015-01-01 00:00:00

  • Tissue engineered hydrogels supporting 3D neural networks.

    abstract::Promoting nerve regeneration requires engineering cellular carriers to physically and biochemically support neuronal growth into a long lasting functional tissue. This study systematically evaluated the capacity of a biosynthetic poly(vinyl alcohol) (PVA) hydrogel to support growth and differentiation of co-encapsulat...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2018.11.044

    authors: Aregueta-Robles UA,Martens PJ,Poole-Warren LA,Green RA

    更新日期:2019-09-01 00:00:00

  • Protein-imprinted polysiloxane scaffolds.

    abstract::Molecular imprinting is a technique used to create specific recognition sites on the surface of materials. Although widely developed for chromatographic separation of small molecules, this approach has not been adequately investigated for biomaterial applications. Thus, the objective of these experiments was to explor...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2007.01.003

    authors: Lee K,Itharaju RR,Puleo DA

    更新日期:2007-07-01 00:00:00

  • Tumor microenvironment targeting with dual stimuli-responsive nanoparticles based on small heat shock proteins for antitumor drug delivery.

    abstract::Tumour microenvironment (TME)-targeting nanoparticles (NPs) were developed based on Methanococcus jannaschii small heat shock proteins (Mj-sHSPs). Transactivator of transcription (TAT) were modified on the surface of Mj-sHSPs (T-HSPs) to enhance their cellular internalization ability (CIA), and a pH/enzyme dual sensit...

    journal_title:Acta biomaterialia

    pub_type: 杂志文章

    doi:10.1016/j.actbio.2020.07.031

    authors: Shi K,Wang Y,Zhou X,Gui H,Xu N,Wu S,He C,Zhao Z

    更新日期:2020-09-15 00:00:00