Abstract：
:Deeper understanding of the bacteriostatic and bactericidal mechanisms of antimicrobial peptides (AMPs) should help in the design of new antibacterial agents. Over several decades, a variety of biochemical assays have been applied to bulk bacterial cultures. While some of these bulk assays provide time resolution of the order of 1min, they do not capture faster mechanistic events. Nor can they provide subcellular spatial information or discern cell-to-cell heterogeneity within the bacterial population. Single-cell, time-resolved imaging assays bring a completely new spatiotemporal dimension to AMP mechanistic studies. We review recent work that provides new insights into the timing, sequence, and spatial distribution of AMP-induced effects on bacterial cells.
journal_name
Trends Microbioljournal_title
Trends in microbiologyauthors
Choi H,Rangarajan N,Weisshaar JCdoi
10.1016/j.tim.2015.11.004subject
Has Abstractpub_date
2016-02-01 00:00:00pages
111-122issue
2eissn
0966-842Xissn
1878-4380pii
S0966-842X(15)00266-8journal_volume
24pub_type
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