当前位置： SCI文献检索 > LIFE SCIENCES期刊下所有文献 > MiR-216b-5p inhibits cell proliferation in human breast cancer by down-regulating HDAC8 expression.

MiR-216b-5p inhibits cell proliferation in human breast cancer by down-regulating HDAC8 expression.

Abstract：

AIM:Over-expression of histone deacetylase 8 (HDAC8) has been demonstrated in breast cancer. But the underlying molecular mechanism of HDAC8 on the progression of breast cancer remains unknown. MicroRNAs (miRs) are proposed as important molecules in cancer progression by targeting specific oncogenes or tumor-suppressor genes. Our overall objective was to assess the miR-216b-5p role on HDAC8; and its impacts on breast cancer (BC) progression. MAIN METHODS:We acquired cancerous and noncancerous tissues from Iran Tumor Bank (I.T.B). The MDA-MB-231, MCF-7 and MCF-10A BC cell lines were also purchased. The tissue and cell line expression levels of miR-216b-5p and HDAC8 were determined by quantitative real-time PCR (qPCR). We next measured protein levels of HDAC8 by Western blotting assay. The cell cycle, cell proliferation, and colony formation assay were determined. Finally, we investigated the role of HDAC8 using a knockout vector; and confirmed the targeting of 3' untranslated region (3'-UTR) of HDAC8 through miR-216b-5p using a luciferase reporter assay. KEY FINDINGS:Our results demonstrated a significant decrease in miR-216b-5p, and remarkable increase in HDAC8 levels within human breast cancer tissues and cell lines. The lower levels of miR-216b-5p were negatively correlated with lymph node metastasis and advanced tumor size. The overexpression of miR-216b-5p in BC cell lines inhibited cellular proliferation and progression. HDAC8 was directly down-regulated by miR-216b-5p and knockout of HDAC8 showed the similar effects as miR-216b-5p overexpression. SIGNIFICANCE:Briefly, HDAC8 is an oncogene that accelerate breast cancer proliferation and progression and miR-216b-5p modulates those functions by binding to HDAC8 3'-UTR.

journal_name

Life Sci

journal_title

Life sciences

authors

Menbari MN,Rahimi K,Ahmadi A,Elyasi A,Darvishi N,Hosseini V,Mohammadi-Yeganeh S,Abdi M

doi

10.1016/j.lfs.2019.116945

subject

Has Abstract

pub_date

2019-11-15 00:00:00

pages

116945

eissn

0024-3205

issn

1879-0631

pii

S0024-3205(19)30872-0

journal_volume

237

pub_type

杂志文章

相关文献

LIFE SCIENCES文献大全
  • Parathyroid hormone-related protein and glucocorticoid receptor beta are regulated by cortisol in the kidney of male mice.

    abstract：AIMS:Parathyroid hormone-related protein (PTHrP) is a peptide growth factor produced in a wide range of tissues from brain and parathyroid, to kidney and uterus. The purpose of this study was to determine whether the adrenal cortical hormones, hydrocortisone (cortisol), modulate PTHrP expression and glucocorticoid rece...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2011.08.001

    authors： Yoo YM,Baek MG,Jung EM,Yang H,Choi KC,Yu FH,Jeung EB

    更新日期：2011-10-24 00:00:00

  • The nature of prooxidant activity of vitamin C.

    abstract：:It was recently reported that vitamin C (500 mg/day for 6 weeks) administered as a dietary supplement to healthy humans exhibits a prooxidant, as well as an antioxidant effect in vivo. Here we show that high intakes of vitamin C (500 mg/kg b.w. for 4 days) in the rat are able to markedly induce hepatic cytochrome P450...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(99)00167-8

    authors： Paolini M,Pozzetti L,Pedulli GF,Marchesi E,Cantelli-Forti G

    更新日期：1999-01-01 00:00:00

  • Nicotine administration to rats: methodological considerations.

    abstract：:The effects of nicotine on normal physiological function are of increasing concern. Preliminary to studies on the effects of prenatal exposure to nicotine, we examined methods of administering nicotine to rats. Drinking water containing nicotine was not palatable to rats and was an unsatisfactory method in our hands, ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(87)90020-8

    authors： Murrin LC,Ferrer JR,Zeng WY,Haley NJ

    更新日期：1987-04-27 00:00:00

  • Effects of cannabinoid receptor ligands on LPS-induced pulmonary inflammation in mice.

    abstract：:The effects of cannabinoid receptor agonists WIN 55,212-2, delta9-tetrahydrocannabinol (delta9-THC), arachidonoylethanolamide (anandamide) and palmitoylethanolamide on lipopolysaccharide (LPS) -induced bronchopulmonary inflammation in mice were investigated. WIN 55,212-2 and delta9-THC induced a concentration-dependen...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(98)00324-5

    authors： Berdyshev E,Boichot E,Corbel M,Germain N,Lagente V

    更新日期：1998-01-01 00:00:00

  • Biotransformation in monkey brain: coupling of sulfation to glutathione conjugation.

    abstract：:Phenol sulfotransferase (PST, EC 2.8.2.1) and glutathione-S-transferase (GST, EC 2.5.1.18), the phase II biotransformation enzymes inactivate many exo- and endogenous compounds. The effect of PST substrates (catecholamines, simple phenols, selected phenolic drugs) and PST products (phenolic sulfates) on GST activity w...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(97)00807-2

    authors： Baranczyk-Kuzma A,Sawicki J

    更新日期：1997-01-01 00:00:00

  • Protective effects of oleanolic acid oligoglycosides on ethanol- or indomethacin-induced gastric mucosal lesions in rats.

    abstract：:We examined the effects of various oleanolic acid oligoglycosides obtained from traditional herbs on ethanol- or indomethacin-induced gastric mucosal lesions in rats and on gastric secretion in pylorus-ligated rats. Test samples were given orally to fasted rats 1 h before absolute ethanol (1.5 ml/rat, p.o.) or indomet...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(98)00426-3

    authors： Matsuda H,Li Y,Murakami T,Yamahara J,Yoshikawa M

    更新日期：1998-01-01 00:00:00

  • Inhibition of shear stress-induced platelet aggregation by cilostazol, a specific inhibitor of cGMP-inhibited phosphodiesterase, in vitro and ex vivo.

    abstract：:Cilostazol(6-[4-(1-cyclohexyl-1H-tetrazol-5-yl)-butoxy]-3,4- dihydro-2(1H)-quinolinone) selectively inhibits cGMP-inhibited phosphodiesterase (PDE3) and is a potent inhibitor of platelet aggregation induced by various agonists. Effect of cilostazol on shear stress-induced human platelet aggregation (SIPA) was examined...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(97)00986-7

    authors： Minami N,Suzuki Y,Yamamoto M,Kihira H,Imai E,Wada H,Kimura Y,Ikeda Y,Shiku H,Nishikawa M

    更新日期：1997-01-01 00:00:00

  • Effects of calcium channel antagonists on the induction of nitric oxide synthase in cultured cells by immunostimulants.

    abstract：:We investigated whether calcium channel antagonists would alter the induction of nitric oxide (NO) synthesis by bacterial lipopolysaccharide (LPS) alone or in combination with interferon-gamma (IFN gamma) in cultured J774 macrophages, rat vascular smooth muscle cells, rat renal mesangial cells, and rat cardiac myocyte...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(95)02163-d

    authors： Hattori Y,Kasai K,So S,Hattori S,Banba N,Shimoda S

    更新日期：1995-01-01 00:00:00

  • A-350619: a novel activator of soluble guanylyl cyclase.

    abstract：:Nitric oxide (NO) is a key mediator in many physiological processes and one of the major receptors through which NO exerts its effects is soluble guanylyl cyclase. Guanylyl cyclase converts GTP to cyclic GMP as part of the cascade that results in physiological processes such as smooth muscle relaxation, neurotransmiss...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(02)02361-5

    authors： Miller LN,Nakane M,Hsieh GC,Chang R,Kolasa T,Moreland RB,Brioni JD

    更新日期：2003-01-17 00:00:00

  • Inhibition of Sendai virus by various snake venom.

    abstract：:Viperid, elapid and crotalid snake venoms were screened in vitro for antiviral activity against Sendai virus. The hemolysis of 10(8) human erythrocytes in 1 ml, caused by 70 HAU of Sendai virus, was abolished when the virions were pretreated with 10 ug of the viperid venom of Echis coloratus, and was considerably dimi...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(92)90015-h

    authors： Borkow G,Ovadia M

    更新日期：1992-01-01 00:00:00

  • Effect of fish oil and vitamin E on ethanol-induced changes in membrane ATPases.

    abstract：:We evaluated whether fish oil or vitamin E administration affected ethanol-induced changes in membrane ATPases. Male Wistar rats (225-250 g) were fed, through a gastric tube a liquid diet containing fish oil (25% of calories) and ethanol for one month. Another group of animals was given supplemental vitamin E (300 u/k...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(94)00545-1

    authors： Nanji AA,Sadrzadeh SM

    更新日期：1994-01-01 00:00:00

  • LncRNA HOX transcript antisense RNA accelerated kidney injury induced by urine-derived sepsis through the miR-22/high mobility group box 1 pathway.

    abstract：OBJECTIVE:This study investigated the role of long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in kidney injury induced by urine-derived sepsis (US). MATERIALS AND METHODS:An Escherichia coli suspension was injected into the distal ureter of adult male Sprague Dawley rats to establish a US model. Lipo...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2018.08.041

    authors： Shen J,Zhang J,Jiang X,Wang H,Pan G

    更新日期：2018-10-01 00:00:00

  • The importance of estrogen for bone protection in experimental hyperthyroidism in human osteoblasts.

    abstract：:Triiodothyronine (T3) and estrogen (E2) play important roles in the bone remodeling process and signaling of receptor activator of the nuclear factor-kappa β (RANKL) and osteoprotegerin (OPG) expressed by osteoblasts. However, little is known of the molecular action of these hormones in conditions of hyperthyroidism a...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2019.116556

    authors： Olímpio RMC,Moretto FCF,De Sibio MT,de Oliveira M,Mathias LS,Gonçalves BM,Deprá IC,Tilli HP,Rodrigues BM,Saraiva PP,Maria DA,Nogueira CR

    更新日期：2019-08-15 00:00:00

  • Suppressive effects of Active Hexose Correlated Compound on the increased activity of hepatic and renal ornithine decarboxylase induced by oxidative stress.

    abstract：:Active Hexose Correlated Compound (AHCC), an extract derived from fungi of Basidiomycetes family has been shown to act as a biological response modifier in various disorders. In our present study, ferric nitrilotriacetate (Fe-NTA), which generates hydroxyl radicals in vivo, was given intraperitoneally to rats and AHCC...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2003.06.038

    authors： Ye SF,Ichimura K,Wakame K,Ohe M

    更新日期：2003-12-19 00:00:00

  • Rational design of a GLP-1/GIP/Gcg receptor triagonist to correct hyperglycemia, obesity and diabetic nephropathy in rodent animals.

    abstract：AIMS:To design and screen a potent GLP-1/GIP/Gcg receptors triagonist with therapeutic potential in rodent animals with diabetes and obesity. MAIN METHODS:First, we obtained a 12-mer dual GIP/Gcg receptor agonist from a large combinatorial peptide library via high-throughput screening technique and then fused to the E...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.118339

    authors： Cui J,Shang A,Wang W,Chen W

    更新日期：2020-11-01 00:00:00

  • mCPP but not TFMPP is an antagonist at cardiac 5HT3 receptors.

    abstract：:The prototypic arylpiperazines, meta-chlorophenylpiperazine (mCPP), meta-trifluoromethylphenylpiperazine (TFMPP) and quipazine are widely studied serotonergic ligands with nonselective effects at 5HT1 and 5HT2 receptor subtypes. The present study was designed to compare the affinities of these arylipiperazines at 5HT3...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(92)90372-v

    authors： Robertson DW,Bloomquist W,Wong DT,Cohen ML

    更新日期：1992-01-01 00:00:00

  • Intestinal transport of pyridoxine in experimental renal failure.

    abstract：:Renal failure (RF) has been shown to alter intestinal transport of a number of nutrients. We studied jejunal absorption of pyridoxine (B6) in rats rendered azotemic by subtotal nephrectomy (RF group) and compared the results with those obtained in normal rats subjected to sham operation (controls) and animals pair-fed...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(89)90053-2

    authors： Barbari A,Vaziri ND,Benavides I,Chen YT,Said H,Pahl MV

    更新日期：1989-01-01 00:00:00

  • A role for calcium/calmodulin kinase in insulin stimulated glucose transport.

    abstract：:Previous research has shown that the CAMK (calcium/calmodulin dependent protein kinase) inhibitor, KN62, can lead to reductions in insulin stimulated glucose transport. Although controversial, an L-type calcium channel mechanism has also been hypothesized to be involved in insulin stimulated glucose transport. The pur...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2003.06.041

    authors： Wright DC,Fick CA,Olesen JB,Lim K,Barnes BR,Craig BW

    更新日期：2004-01-02 00:00:00

  • Effect of stobadine on cardiac injury induced by ischemia and reperfusion.

    abstract：:The aim of this work was to evaluate the effect of the antioxidant stobadine on ischemia/reperfusion-induced injury of the isolated rat heart. Experiments were performed according to Langendorff. Ischemia was induced by stop-flow lasting 30 minutes and the duration of repefusion was 30 minutes. Reperfusion of the isch...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(99)00450-6

    authors： Knezl V,Sotníková R,Okruhlicová L,Navarová J

    更新日期：1999-01-01 00:00:00

  • Thalidomide derivatives and the immune system. 6. Effects of two derivatives with no obvious teratogenic potency on the pattern of integrins and other surface receptors on blood cells of marmosets.

    abstract：:The two thalidomide (Thd) derivatives beta-EM12 and phthalimidophthalimide (Phtpht), which exhibit no obvious teratogenicity, were tested for their ability to induce changes in the pattern of lymphocyte subpopulations, and especially changes in integrin receptors, in marmosets (Callithrix jacchus). Previously, Thd and...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(95)02293-7

    authors： Nogueira AC,Neubert R,Felies A,Jacob-Müller U,Frankus E,Neubert D

    更新日期：1996-01-01 00:00:00

  • Activin receptors are expressed on human lung fibroblast and activin A facilitates fibroblast-mediated collagen gel contraction.

    abstract：:Activin A is a member of the transforming growth factor-beta superfamily that exerts its diverse biological effects through bindings to activin specific transmembrane serine/threonine kinase receptors. The fibroblast-mediated contraction of a collagen gel is thought to be a model of part of the wound-repair response a...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(00)00480-x

    authors： Ohga E,Matsuse T,Teramoto S,Ouchi Y

    更新日期：2000-03-01 00:00:00

  • Serotonergic mediation of fenfluramine discriminative stimuli in fawn-hooded rats.

    abstract：:Fenfluramine, a drug that induces increased synaptic serotonin, was used to train Fawn-Hooded rats in a drug discrimination paradigm. This strain of rats is thought to possess a genetic serotonin storage abnormality. The intent of the study was to see if the Fawn-Hooded rat was similar or dissimilar to the more freque...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：

    authors： Schechter MD

    更新日期：1997-01-01 00:00:00

  • Drug targeting of leptin resistance.

    abstract：:Leptin regulates glucose, lipid and energy homeostasis as well as feeding behavior, serving as a bridge between peripheral metabolically active tissues and the central nervous system (CNS). Indeed, this adipocyte-derived hormone, whose circulating levels mirror fat mass, not only exerts its anti-obesity effects mainly...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2015.05.012

    authors： Santoro A,Mattace Raso G,Meli R

    更新日期：2015-11-01 00:00:00

  • Morphine treatment in vitro or in vivo decreases phagocytic functions of murine macrophages.

    abstract：:Studies were performed to compare in vitro and in vivo effects of morphine on the phagocytic function of murine peritoneal macrophages. Macrophage monolayers were incubated with Candida albicans for 30 min in the absence of autologous serum. Morphine added in vitro was found to decrease both the phagocytic activity (p...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(93)90122-j

    authors： Rojavin M,Szabo I,Bussiere JL,Rogers TJ,Adler MW,Eisenstein TK

    更新日期：1993-01-01 00:00:00

  • Schisantherin A causes endothelium-dependent and -independent vasorelaxation in isolated rat thoracic aorta.

    abstract：AIMS:Schisandra is a good choice in Traditional Chinese Medicine for the therapy of cardiovascular diseases, but whether it contains a or some specific component (s) responsible these effects are still unclear. In the present study, we explored whether Schisantherin A (SCA) causes vasorelaxation in isolated rat thoraci...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2020.117357

    authors： Yang S,Xu Z,Lin C,Li H,Sun J,Chen J,Wang C

    更新日期：2020-03-15 00:00:00

  • Ubiquitination in rheumatoid arthritis.

    abstract：:Rheumatoid arthritis is a chronic, inflammatory joint disease leading to inflammation of synovial membrane that lines the joints. This inflammation further progresses and results in destruction of joints and surrounding cartilages. The underlying factors can be oxidative stress, pro-inflammatory mediators, imbalance a...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2020.118459

    authors： Behl T,Chadha S,Sachdeva M,Kumar A,Hafeez A,Mehta V,Bungau S

    更新日期：2020-11-15 00:00:00

  • The non-neuronal cholinergic system as novel drug target in the airways.

    abstract：:The parasympathetic nervous system is a key regulator of the human organism involved in the pathophysiology of various disorders through cholinergic mechanisms. In the lungs, acetylcholine (ACh) released by vagal nerve endings stimulates muscarinic receptors thereby increasing airway smooth muscle tone. Contraction of...

    journal_title：Life sciences

    pub_type： 杂志文章,评审

    doi：10.1016/j.lfs.2012.08.030

    authors： Pieper MP

    更新日期：2012-11-27 00:00:00

  • beta-Endorphin immunoreactive materials in human plasma determined by a multiple radioimmunoassay system.

    abstract：:Extracts of plasma obtained from adult humans before and immediately after physical stress were subjected to high pressure liquid chromatography (HPLC); extracts or HPLC fractions, resp., were analysed for the presence of human beta-endorphin (beta H-endorphin) or fragments thereof by several radioimmunoassays using a...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/0024-3205(83)90451-4

    authors： Wiedemann K,Teschemacher H

    更新日期：1983-01-01 00:00:00

  • Curcumin ameliorates alveolar epithelial injury in a rat model of chronic obstructive pulmonary disease.

    abstract：AIMS:To investigate the effects of curcumin on alveolar epithelial injury in a rat model of chronic obstructive pulmonary disease (COPD) and its potential mechanism. MAIN METHODS:The rat COPD model was established by cigarette smoke exposure combined with intratracheal administration of lipopolysaccharide. Thirty-eigh...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/j.lfs.2016.09.001

    authors： Zhang M,Xie Y,Yan R,Shan H,Tang J,Cai Y,Yin J,Chen M,Zhang J,Yang X,Zhang Q,Li Y

    更新日期：2016-11-01 00:00:00

  • Effects of transforming growth factor beta1 on cell growth and parathyroid hormone-related protein in Walker 256 tumor cells.

    abstract：:Hypercalcemic strains of the rat Walker 256 (W256) tumor synthesize parathyroid hormone-related protein (PTHrP) and at least one of them produces an ill-defined transforming growth factor activity. We tested the production of transforming growth factor (TGF) beta by a hypercalcemic W256 tumor strain, and assessed its ...

    journal_title：Life sciences

    pub_type： 杂志文章

    doi：10.1016/s0024-3205(99)00437-3

    authors： Benítez-Verguizas J,Loarte D,de Miguel F,Esbrit P

    更新日期：1999-01-01 00:00:00