Abstract:
OBJECTIVE:To study the myocardial benefit effect and mechanism of paeoniflorin on myocardial ischemia reperfusion injury (MIRI) in rats. METHODS:Hundred SD rats were randomly divided into 5 groups: sham group, model group, Paeoniflorin (15 mg/kg) group, Paeoniflorin (30 mg/kg) group, and Paeoniflorin (60 mg/kg) group. Myocardial ischemia reperfusion model was established in each group except the sham group. The myocardial infarction and morphological changes were measured by the TTC staining and HE staining respectively. Myocardial caspase-3 was detected by immunohistochemistry. In addition, the protein levels of Bcl-2 and Bax and the expression ratio of p-erk, p-jnk, and p-p38 were detected by Western blot. Myocardial superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured by the assay kit. RESULTS:Paeoniflorin (30 mg/kg) and Paeoniflorin (60 mg/kg) can obviously alleviate myocardial infarction caused by MIRI (p < 0.05). HE staining showed that the myocardial morphology in the treatment group was obviously better than that in the model group. WB and immunohistochemistry showed that Paeoniflorin (30 mg/kg) and Paeoniflorin (60 mg/kg) can significantly increase the reduced protein level of bcl-2 (p < 0.05) and reduce the increased protein level of caspase3, bax -p-erk, p-jnk, and p-p38 caused by MIRI (p < 0.05). The activity of SOD was increased and the level of MDA was decreased after Paeoniflorin treatment. CONCLUSION:Paeoniflorin preconditioning has a protective effect on MIRI in rats. Its mechanism is related to reducing oxidative stress and apoptosis by inhibiting the expression of apoptosis-related signaling pathway.
journal_name
Pharmacologyjournal_title
Pharmacologyauthors
Wu F,Ye B,Wu X,Lin X,Li Y,Wu Y,Tong Ldoi
10.1159/000503583subject
Has Abstractpub_date
2020-01-01 00:00:00pages
281-288issue
5-6eissn
0031-7012issn
1423-0313pii
000503583journal_volume
105pub_type
杂志文章相关文献
PHARMACOLOGY文献大全abstract::L-Carnitine (oral route) significantly corrects the muscle hypocontractility and hypoexcitability induced in the rat after 5 consecutive days of fasting. This effect is interpreted on the basis of the dual role of L-carnitine as cofactor in the transport of long-chain fatty acids into the mitochondria and as a detoxif...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139026
更新日期:1993-01-01 00:00:00
abstract::The cardiovascular effects of SKP-450, a newly synthesized potassium channel activator, and its two major metabolites SKP-818 and SKP-310 were evaluated on isolated rat aorta and in freely moving rats and anesthetized beagle dogs. The rank order of potency in relaxing rat aorta precontracted with norepinephrine was SK...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028188
更新日期:1998-03-01 00:00:00
abstract::It can be readily shown in vitro that various compounds inhibit platelet aggregation but it has been difficult to demonstrate in vivo. A method is suggested here to enable in vivo testing of these compounds; the method utilizes relatively low doses of sodium arachidonate, made possible by the ligation of the pterygopa...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137350
更新日期:1980-01-01 00:00:00
abstract::This study examined effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 and SRIH-28) on growth and function of pituitary somatotropes (GH cells) and lactotropes (PRL cells). Male rats received three i.c.v. injections (1 microgram/5 microliters) of SRIH-14 or SRIH-28 every second day. Blood...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028223
更新日期:1998-07-01 00:00:00
abstract::We examined the properties of the drug interaction between morphine and 5-HT(3) receptor antagonist at the spinal level. The nociceptive state was induced by subcutaneously injecting formalin solution (5%, 50 microl) into the hindpaw of the rats. Intrathecal morphine and m-CPBG (5-HT(3) receptor agonist) dose-dependen...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000094763
更新日期:2006-01-01 00:00:00
abstract::Hepatic lipid accumulation and apoptosis is elevated in patients with non-alcoholic steatohepatitis and is closely associated with severity. Saturated fatty acid palmitate stimulates lipid accumulation and apoptosis in hepatocytes. In the present study, we examined bee-bee tree oil (BO)-mediated protective effects on ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000487892
更新日期:2018-01-01 00:00:00
abstract::The aim of this study was to compare the effects of a new thromboxane synthase inhibitor, camonagrel, on platelet aggregation and platelet-subendothelium interaction under flow conditions, in comparison with a standard thromboxane synthase inhibitor (dazoxiben) and a cyclooxygenase inhibitor (acetylsalicylic acid). Wi...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000071266
更新日期:2003-09-01 00:00:00
abstract::Cinnamedrine is an active ingredient in preparations used to relieve dysmenorrhea. It has been reported to have local anesthetic properties in nerve. This property prompted us to evaluate the effects of cinnamedrine on the cardiac action potential. Cinnamedrine (10-35 microM) significantly reduced the overshoot and ma...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138281
更新日期:1987-01-01 00:00:00
abstract:AIM:To evaluate the efficacy and safety of 2 analogs of L-carnitine on rats made insulin resistant by a high-fructose diet. METHODS:Using rats made insulin resistant by a high-fructose diet, we investigated the impact of 2 analogs of L-carnitine (25 mg/kg) and L-carnitine (250 mg/kg) on glucose, triglycerides and chol...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000328772
更新日期:2011-01-01 00:00:00
abstract::Two groups of rats were used to examine the effect of pioglitazone, a peroxisome proliferator-activated receptor gamma (PPARgamma) agonist, on rat hearts using an in vivo model of ischemia-reperfusion (I/R) to elucidate potential mechanisms. One group was the 30-min reperfusion group, which was further subdivided into...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000100870
更新日期:2007-01-01 00:00:00
abstract::The activity of myocardial adenylate cyclase from two age groups (10-11 and 18-19 weeks old, respectively) of male spontaneously hypertensive rats (SHR) and their age-matched normotensive Wistar-Kyoto rats (WKY) was examined. Stimulation of the enzyme by isoproterenol and 5'-guanylyl-imidodiphosphate (GMP-PNP) was dec...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137488
更新日期:1981-01-01 00:00:00
abstract::The endocrine (plasma renin activity, insulin and ADH) and hemodynamic responses (heart rate and mean arterial pressure) to isoprenaline infusion were examined in conscious deoxycorticosterone-salt hypertensive rats (DS) and compared with uninephrectomized-salt control rats (US). A dose-related rise in plasma renin ac...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138522
更新日期:1989-01-01 00:00:00
abstract::Using the method of staining mucus with Alcian blue and destaining it with magnesium chloride, it was found that intragastric administration of carbenoxolone in mice did not significantly affect duodenal mucus synthesis at doses which remarkably increased gastric mucus synthesis. However, in vitro study showed that ca...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138201
更新日期:1986-01-01 00:00:00
abstract::A variety of prostaglandin synthetase inhibitors are cooxygenated during arachidonic acid peroxidation catalyzed by rat renal medulla prostaglandin synthetase or soybean lipoxygenase. Phenylbutazone, aminopyrine, 1,3-diphenylisobenzofuran, paracetamol, p-aminophenol, p-phenetidine and other o- and m-substituted aminop...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137880
更新日期:1983-01-01 00:00:00
abstract:BACKGROUND:Targeting inflammation in patients with coronary artery disease and/or acute myocardial infarction (AMI) is a matter of debate. Methotrexate (MTX) is one of the most widely used immunosuppressants. Cardiovascular Inflammation Reduction Trial (CIRT) recently failed to demonstrate reduced cardiovascular events...
journal_title:Pharmacology
pub_type: 新闻
doi:10.1159/000511279
更新日期:2020-11-20 00:00:00
abstract::In this study, we used a novel conscious dog model to evaluate the uroselectivity of selected alpha 1-antagonists either approved for human use or in clinical development for the treatment of symptomatic benign prostatic hyperplasia (BPH) and compared those results to their in vitro binding and functional affinities a...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139451
更新日期:1996-12-01 00:00:00
abstract::The systemic and coronary hemodynamic effects of the nitrovasodilator, pirsidomine, were compared with SIN-1, nitroprusside, and nitroglycerin. Four groups consisting of 32 experiments were performed in 17 conscious dogs chronically instrumented for measurement of aortic and left ventricular pressure, left ventricular...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139372
更新日期:1996-02-01 00:00:00
abstract::Protein phosphatase inhibitors, e.g. cantharidin, exert positive inotropic effects in mammalian heart preparations. Endothall, a synthetic herbicide which is chemically related to cantharidin, inhibits protein phosphatase activities in mouse liver preparations. However, the cardiac effects of endothall have hitherto n...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028379
更新日期:2000-07-01 00:00:00
abstract::High-affinity (104 +/- 18 pmol/l) and high-density (204 +/- 25 fmol/mg) angiotensin II (AII) binding sites have been identified in Xenopus laevis heart membranes. Competition binding of [125I]Sar1,Ile8 angiotensin (SIA) to these receptors by peptide analogs selective for the mammalian AII receptor subtypes AT1 and AT2...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139186
更新日期:1994-04-01 00:00:00
abstract::The present study was aimed at investigating the effects of diabetes on the cavernosal smooth muscle relaxations mediated by adenosine and adenosine triphosphate (ATP) in tissues obtained from men and rats. Adenosine- and ATP-induced relaxant responses showed an enhanced sensitivity with an unaltered effectiveness in ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000028354
更新日期:2000-02-01 00:00:00
abstract::The influence of hyperthyroidism on the functional vascular responsiveness of isolated coronary and renal resistance vessels was investigated. Hyperthyroidism was established by feeding rats for 1 and 4 weeks with 5 mg/kg L-thyroxine (T4)-containing rat chow. Preparations of either coronary or renal resistance vessels...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139519
更新日期:1997-09-01 00:00:00
abstract:BACKGROUND:Cassia angustifolia L. (senna) is traditionally used as a laxative. Its major components are sennosides that are responsible for the laxative effect. Senna is recommended for the short-term treatment of acute constipation. Nevertheless people use its preparations as self-medication, often for long periods, t...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000331858
更新日期:2011-01-01 00:00:00
abstract::The electrophysiological and mechanical effects of HNS-32, a novel azulene-1-carboxamidine derivative with antiarrhythmic activity, were studied in isolated guinea-pig myocardial preparations. HNS-32 (10(-6)-10(-4) mol/l) concentration-dependently decreased the maximum rate of rise (V(max)) of action potential in isol...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000056148
更新日期:2002-01-01 00:00:00
abstract::We established an Ascaris-induced biphasic skin allergic reaction in mice. In the early-phase reaction (EPR), mast cell degranulation was observed, and tranilast inhibited ear edema. In mast-cell-deficient mice (WBB6F(1)-W/W(V) mice), ear edema in the EPR disappeared, whereas that in the late-phase reaction (LPR) rema...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000056117
更新日期:2001-01-01 00:00:00
abstract:AIM:Superfluous reactive nitrogen and oxygen species generation is implicated in the damage of tissues at sites of inflammation where activated neutrophils and macrophages are involved. This study was conducted to investigate whether the beneficial effects of carvedilol involve modulation of respiratory burst, degranul...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000097818
更新日期:2007-01-01 00:00:00
abstract::The marijuana plant Cannabis sp. and its derivatives and analogues, known as cannabinoids (CBs), induce many effects throughout the whole body. Herein we briefly review the gastrointestinal (GI) pharmacology of CBs, with special focus on motor function. Some drugs are available to treat nausea and emesis, and evidence...
journal_title:Pharmacology
pub_type: 杂志文章,评审
doi:10.1159/000339072
更新日期:2012-01-01 00:00:00
abstract::Glutathione S-transferases (GSTs) detoxify diverse electrophilic chemicals, including anticancer drugs. Containing at least 100 microM total GST, the adult rat liver has abundant alpha and mu class GST isoenzymes. We utilized primary cultured rat hepatocytes, maintained in chemically defined medium, to examine direct ...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000139312
更新日期:1995-06-01 00:00:00
abstract::Three highly reproducible experiments on drug interaction in normal human volunteers provided anomalous results: chronic halofenate administration shortened plasma antipyrine and bishydroxycoumarin half-lives but prolonged plasma warfarin half-lives. This dissociation in the effect produced by a chronically administer...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000136892
更新日期:1975-01-01 00:00:00
abstract::In an experimental model of haemorrhagic shock that causes 100% mortality in rats within 30 min, the intravenous bolus injection (20 micrograms/kg) of sulfated cholecystokinin octapeptide (CCK-8) induces a prompt and sustained rise in blood pressure and pulse amplitude, all treated animals still surviving at the end o...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000138480
更新日期:1988-01-01 00:00:00
abstract::In gross behavioral observation the minimum effective dose of zopiclone was intravenously 0.06-0.25 mg/kg and intragastrically 1-4 mg/kg in rhesus monkeys. Zopiclone suppressed barbital withdrawal signs of rhesus monkeys at a single dose of 16 mg/kg or more. The suppressing effect of zopiclone 32 mg/kg i.g. was compar...
journal_title:Pharmacology
pub_type: 杂志文章
doi:10.1159/000137930
更新日期:1983-01-01 00:00:00