PSA modification of NCAM supports the survival of injured retinal ganglion cells in adulthood.

Abstract:

:Neural cell adhesion molecule (NCAM) is known as the cell surface glycoprotein, and it belongs to the immunoglobulin superfamily of adhesion molecules. Polysialic acid (PSA) is a carbohydrate attached to NCAM via either of two specific sialyltransferases: ST8SiaII and ST8SiaIV. Polysialylated neural cell adhesion molecule (PSA-NCAM) mediates cell interactions, plays a role in axon growth, migration, synaptic plasticity during development and cell regeneration. Some evidence has shown that PSA-NCAM supports the survival of neurons. It was demonstrated that PSA-NCAM is present in abundance in the retina during development and in adulthood. The aim of this study was to investigate whether PSA-NCAM promotes retinal ganglion cell (RGC) survival in transgenic mice with deficiencies in sialyltransferases or NCAM or after the administration of endoneuraminidase (Endo-N). RGC injury was induced by intravitreal administration of kainic acid (KA). These studies showed that injection of Endo-N after 14 days enhances the toxicity of KA to RGCs in wild-type (WT) mice by 18%. In contrast, in knockout mice (ST8SiaII-/-, ST8SiaIV-/-, NCAM-/-), survival of RGCs after KA injury did not change. Deficiencies of either ST8SiaII or ST8SiaIV did not influence the level of PSA-NCAM in the adult retina, however, in neonatal animals, decreased levels of PSA-NCAM were observed. In knockout ST8SiaII-/- adults, a reduced number of RGCs was detected, whereas in contrast, increased numbers of RGCs were noted in NCAM-/- mice. In conclusion, these data demonstrate that PSA-NCAM supports the survival of injured RGCs in adulthood. However, the role of PSA-NCAM in the adult retina requires further clarification.

journal_name

Brain Res

journal_title

Brain research

authors

Lobanovskaya N,Zharkovsky T,Jaako K,Jürgenson M,Aonurm-Helm A,Zharkovsky A

doi

10.1016/j.brainres.2015.08.008

subject

Has Abstract

pub_date

2015-11-02 00:00:00

pages

9-17

eissn

0006-8993

issn

1872-6240

pii

S0006-8993(15)00612-5

journal_volume

1625

pub_type

杂志文章
  • Insulin inhibits pyramidal neurons in hippocampal slices.

    abstract::Recent studies have confirmed the presence of insulin receptors in the rat brain although their function has still not been well defined. The present study explores the possibility that insulin receptors in the brain can alter or contribute to central neurotransmission. Insulin caused a dose-dependent inhibition of hi...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(84)91028-x

    authors: Palovcik RA,Phillips MI,Kappy MS,Raizada MK

    更新日期:1984-08-20 00:00:00

  • Vestibular afferents to the motoneurons of glossopharyngeal and vagus nerves in the frog, Rana esculenta.

    abstract::The aim of this work was to study whether the vestibular afferent fibers establish direct connections with the motoneurons of glossopharyngeal and vagus nerves of the frog, Rana esculenta. In anaesthetized animals the vestibulocochlear nerve and the common root of glossopharyngeal-vagus and accessory (IX-X-XI) nerves ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2009.06.048

    authors: Deák A,Bácskai T,Veress G,Matesz C

    更新日期:2009-08-25 00:00:00

  • The distribution of normal brain water content in Wistar rats and its increase due to ischemia.

    abstract::The aim of this study was to describe the normal distribution of the water content in several regions of the rat brain and furthermore to determine if global ischemia results in a variable increase in water content in these regions. Water content was determined in 40 regions; in 8 unaffected brains by gravimetry and i...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(96)01165-1

    authors: Schwab M,Bauer R,Zwiener U

    更新日期:1997-02-21 00:00:00

  • Involvement of TNF-alpha in glutamate-induced apoptosis in a differentiated neuronal cell line.

    abstract::We examined the involvement of tumor necrosis factor (TNF)-alpha on glutamate-induced cytotoxicity in a differentiated neuronal cell line. In this study, we used nerve growth factor (NGF)-differentiated PC12h cells. Glutamate cytotoxicity was assessed using the MTS and TUNEL assays. To detect TNF-alpha levels in cultu...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2006.09.006

    authors: Kogo J,Takeba Y,Kumai T,Kitaoka Y,Matsumoto N,Ueno S,Kobayashi S

    更新日期:2006-11-29 00:00:00

  • Binding sites for 125I ET-1, ET-2, ET-3 and vasoactive intestinal contractor are present in adult rat brain and neurone-enriched primary cultures of embryonic brain cells.

    abstract::Binding sites for iodinated endothelin (ET)-2, ET-3 and vasoactive intestinal contractor (VIC) were visualised in the adult rat brain using quantitative autoradiography and have a similar anatomical distribution to that of ET-1 and sarafotoxin S6b. Highest densities of binding sites for all 5 labelled peptides were pr...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(91)90201-6

    authors: Davenport AP,Morton AJ

    更新日期:1991-07-19 00:00:00

  • Amantadine increases the extracellular dopamine levels in the striatum by re-uptake inhibition and by N-methyl-D-aspartate antagonism.

    abstract::This study was performed to investigate the mechanism how amantadine increases the extracellular dopamine (DA) levels in the striatum in vivo. Local application of amantadine (1 mM, 40 min) to the striatum through the dialysis membrane significantly increased the extracellular DA levels. Coadministration of nomifensin...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(94)90821-4

    authors: Mizoguchi K,Yokoo H,Yoshida M,Tanaka T,Tanaka M

    更新日期:1994-10-31 00:00:00

  • Noradrenergic lesion of the locus coeruleus increases the firing activity of the medial prefrontal cortex pyramidal neurons and the role of alpha2-adrenoceptors in normal and medial forebrain bundle lesioned rats.

    abstract::Degeneration of noradrenergic neurons in the locus coeruleus (LC) and dysfunction of the prefrontal cortex were regarded as playing a specific role in the occurrence of non-motor symptoms in Parkinson's disease. The present study examined the spontaneous firing rate and firing pattern of medial prefrontal cortex (mPFC...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2010.02.009

    authors: Wang Y,Zhang QJ,Liu J,Ali U,Gui ZH,Hui YP,Wang T,Chen L,Li Q

    更新日期:2010-04-09 00:00:00

  • Proliferation and death of conditionally immortalized neural cells from murine neocortex: p53 alters the ability of neuron-like cells to re-enter the cell cycle.

    abstract::Neurons are distinctive in that they are generally considered to be permanently post-mitotic cells. The oncoprotein p53 is a key regulator in neuronal development, notably in cell proliferation and neuronal death. We hypothesize that p53 maintains the post-mitotic characteristic of differentiated neurons. New lines of...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(02)04119-7

    authors: Miller MW,Peter A,Wharton SB,Wyllie AH

    更新日期:2003-03-07 00:00:00

  • Resistance to anoxic injury in the dorsal columns of adult rat spinal cord following demyelination.

    abstract::In order to examine the relationship between myelination and sensitivity to anoxia in adult white matter, we studied action potential conduction in the spinal cord dorsal column of adult rats in which focal demyelinating lesions had been produced using ethidium bromide/X-irradiation. Acutely isolated spinal cords from...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(97)01171-2

    authors: Imaizumi T,Kocsis JD,Waxman SG

    更新日期:1998-01-01 00:00:00

  • Autoradiographic evidence for a projection from the pretectal nucleus of the optic tract to the dorsal lateral geniculate complex in the cat.

    abstract::A pathway from the pretectal region to the dorsal division of the lateral geniculate body has been studied by autoradiography in adult cats following injection of tritium-labeled amino acids into the meso-diencephalic border zone. The findings suggest that the pretecto-geniculate connection arises in the ipsilateral n...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(80)90861-6

    authors: Graybiel AM,Berson DM

    更新日期:1980-08-11 00:00:00

  • Stimulation-dependent release of adenosine triphosphate from hippocampal slices.

    abstract::Schaffer collaterals of rat and mouse hippocampal slices were stimulated with bursts of pulses (300 Hz for 50 ms, 2-s intervals) for 30-s which caused a stable increase in the size of the population spike known as long-term potentiation. The release of adenosine triphosphate (ATP) was measured with a luciferase-lucife...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(89)90567-2

    authors: Wieraszko A,Goldsmith G,Seyfried TN

    更新日期:1989-04-24 00:00:00

  • Anti-synaptic antibody in allergic encephalomyelitis. I. Neurophysiological studies, in guinea pigs, on the exposed cerebral cortex and peripheral nerves, following immunological challenges with myelin and synaptosomes.

    abstract::The potential evoked at the surface of the cerebral cortex of a guinea pig, by stimulation of the contralateral forepaw, usually consists of an initial double positive wave whose waveform remains unchanged during rapid stimulation. In a guinea pig with experimental allergic encephalomyelitis (EAE) the response is atte...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(75)90350-9

    authors: Lumsden CE,Howard L,Aparicio SR

    更新日期:1975-08-08 00:00:00

  • Processing multidimensional objects under different perceptual loads: the priority of bottom-up perceptual saliency.

    abstract::The role of perceptual load in selective attention to multidimensional objects was investigated by independently manipulating the load along the task-relevant and the task-irrelevant dimensions in the central search array, which was flanked by congruent, incongruent, or neutral peripheral distractors. The relative bot...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2006.07.071

    authors: Wei P,Zhou X

    更新日期:2006-10-09 00:00:00

  • Role of p75NTR in NMDAR-mediated excitotoxic brain injury in neonatal mice.

    abstract:BACKGROUND:Perinatal brain injury in preterm infants is a major cause of neurological handicap. The role of the neurotrophin receptor p75 (p75(NTR)) in the pathogenesis and repair of neonatal excitotoxic brain injury is unknown. Depending on a complex interplay of neurotrophin signalling, p75(NTR) can, in addition to i...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2010.07.095

    authors: Griesmaier E,Schlager G,Wegleiter K,Hermann M,Urbanek M,Simbruner G,Keller M

    更新日期:2010-10-08 00:00:00

  • Circadian vasopressin release from perifused rat suprachiasmatic explants in vitro: effects of acute stimulation.

    abstract::Using a perifusion culture technique, the pattern of vasopressin release from the suprachiasmatic nucleus was studied in vitro under basal and stimulated conditions. Individual rat suprachiasmatic explants released vasopressin in a rhythmic fashion and these rhythms persisted with a circadian period for up to 4 cycles...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(87)90952-8

    authors: Earnest DJ,Sladek CD

    更新日期:1987-10-06 00:00:00

  • Tyrosine phosphorylation of the NR2B subunit of the NMDA receptor in the spinal cord contributes to chronic visceral pain in rats.

    abstract::The roles of spinal N-methyl-d-aspartic acid receptor 2B (NR2B) subunit in central sensitization of chronic visceral pain were investigated. A rat model with irritable bowel syndrome (IBS) was established by colorectal distention (CRD) on post-natal days 8-14. Responses of the external oblique muscle of the abdomen to...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2013.10.008

    authors: Luo XQ,Cai QY,Chen Y,Guo LX,Chen AQ,Wu ZQ,Lin C

    更新日期:2014-01-13 00:00:00

  • Metabolic correlates of lesion-specific plasticity: an in vivo imaging study.

    abstract::High-resolution positron emission tomography (microPET) allows for repeated observations of brain function in the same animal. In a previous study, using [(18)F] fluorodeoxyglucose (FDG) microPET, we demonstrated diminished glucose metabolism and subsequent recovery in the Neostriatum and thalamus ipsilateral to corti...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2003.12.006

    authors: Mir HM,Tatsukawa KJ,Carmichael ST,Chesselet MF,Kornblum HI

    更新日期:2004-03-26 00:00:00

  • Effect of hypothyroidism on the size of spines of pyramidal neurons of the cerebral cortex.

    abstract::We have previously shown that hypothyroidism produces a decrease in the number of spines counted along the apical shafts of pyramidal neurons of the cortex. Nevertheless, other authors have found that when an animal is subjected to some adverse living conditions the size of the spines decreases, making them invisible ...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(85)90057-5

    authors: Berbel PJ,Escobar del Rey F,Morreale de Escobar G,Ruiz-Marcos A

    更新日期:1985-07-01 00:00:00

  • Neuropeptide Y-immunoreactive neurons receive synaptic inputs from dopaminergic axon terminals in the rat neostriatum.

    abstract::Double immunocytochemistry using peroxidase-antiperoxidase and protein A-gold was performed to determine whether neuropeptide Y (NPY) immunoreactive neurons receive synaptic inputs from catecholaminergic axon terminals in the rat neostriatum. Tyrosine hydroxylase-immunoreactive axons were found to be in synaptic conta...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(88)90484-2

    authors: Kubota Y,Inagaki S,Kito S,Shimada S,Okayama T,Hatanaka H,Pelletier G,Takagi H,Tohyama M

    更新日期:1988-08-23 00:00:00

  • Short-term consolidation of visual patterns interferes with visuo-spatial attention: converging evidence from human electrophysiology.

    abstract::In order to investigate the interplay between visuo-spatial attention and central attention, we varied the relative probability (25% vs. 75%) of the responses to lateralized targets in an attentional blink paradigm. When the first target was associated with a less probable response, we observed a larger attentional bl...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2007.09.004

    authors: Robitaille N,Jolicoeur P,Dell'Acqua R,Sessa P

    更新日期:2007-12-14 00:00:00

  • Enhanced acetylcholine release in the hippocampus and cortex during acquisition of an operant behavior.

    abstract::The activity of the septo-hippocampal and nucleus basalis-cortical cholinergic pathways was investigated by measuring changes in the extracellular acetylcholine levels in the hippocampus and parietal cortex, by means of transversal microdialysis, during the acquisition and recall of a positively reinforced operant beh...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(96)00292-2

    authors: Orsetti M,Casamenti F,Pepeu G

    更新日期:1996-06-10 00:00:00

  • A histologically derived stereotaxic atlas and substance P immunohistochemistry in the brain of the least shrew (Cryptotis parva) support its role as a model organism for behavioral and pharmacological research.

    abstract::Chemotherapy is an effective treatment but difficult to tolerate due to side effects like vomiting. Studies on the etiology of chemotherapy-related emesis have implicated brainstem nuclei and the neurotransmitter substance P, among other substrates. Since rodents do not vomit, other species have been necessary as alte...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2007.04.061

    authors: Ray AP,Darmani NA

    更新日期:2007-07-02 00:00:00

  • Ethanol enhancement of GABA-activated chloride current in rat dorsal root ganglion neurons.

    abstract::The acute effects of ethanol on the gamma-aminobutyric acid (GABA)-activated current were studied with the rat dorsal root ganglion neurons in primary culture using the whole-cell patch-clamp technique. GABA produced an inward chloride current, which was composed of an initial transient and a subsequent sustained phas...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(90)90982-h

    authors: Nishio M,Narahashi T

    更新日期:1990-06-04 00:00:00

  • The release of noradrenaline in the locus coeruleus and prefrontal cortex studied with dual-probe microdialysis.

    abstract::The present study was undertaken to investigate and compare the properties of noradrenaline release in the locus coeruleus (LC) and prefrontal cortex (PFC). For that aim the dual-probe microdialysis technique was applied for simultaneous detection of noradrenaline levels in the LC and PFC in conscious rats. Calcium om...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(01)02553-7

    authors: Pudovkina OL,Kawahara Y,de Vries J,Westerink BH

    更新日期:2001-07-06 00:00:00

  • Lesions to the nucleus basalis magnocellularis lower performance but do not block the retention of a previously acquired learning set.

    abstract::Rats were first trained to acquire an olfactory discrimination learning set (ODLS) on 40 olfactory-unique discrimination problems. Following acquisition of ODLS, animals were lesioned bilaterally in the nucleus basalis magnocellularis (nBM) using either quisqualic acid (QUIS) or 192 IgG-saporin (SAP). QUIS animals per...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/j.brainres.2006.12.028

    authors: Bailey AM,Lee JM

    更新日期:2007-03-09 00:00:00

  • Effects of long duration odor exposure on the unit activity of olfactory bulb cells in awake rabbits.

    abstract::Recordings of single unit activity before, during and after a 1-h continuous exposure were made from the mitral cell layer of the olfactory bulbs of awake rabbits. Thirteen complete 1-h exposures were performed using 8 neurons, while a further 6 neurons were lost before the end of the exposure. During the first few mi...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(82)90951-9

    authors: Chaput MA,Panhuber H

    更新日期:1982-10-28 00:00:00

  • The NMDA antagonist, MK-801, suppresses long-term potentiation, kindling, and kindling-induced potentiation in the perforant path of the unanesthetized rat.

    abstract::Antagonism of NMDA-mediated transmission by MK-801 has been shown to block long-term potentiation (LTP) in vitro and delay electrical kindling of the amygdala. The present experiment sought to examine the relationship between synaptic potentiation of the perforant path-granule cell synapse and development of perforant...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(90)90064-i

    authors: Gilbert ME,Mack CM

    更新日期:1990-06-11 00:00:00

  • Differential effects of diet and obesity on high and low affinity sulfonylurea binding sites in the rat brain.

    abstract::The brain contains neurons which alter their firing rates when ambient glucose concentrations change. An ATP-sensitive K+ (Katp) channel on these neurons closes and increases cell firing when ATP is produced by intracellular glucose metabolism. Binding of the antidiabetic sulfonylurea drugs to a site linked to this ch...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(96)00835-9

    authors: Levin BE,Brown KL,Dunn-Meynell AA

    更新日期:1996-11-11 00:00:00

  • Secretion of GDNF by glial cells does not account for the neurotrophic effect of bFGF on dopamine neurons in vitro.

    abstract::The neurotrophic effect of bFGF on dopamine (DA) neurons in vitro depends upon its mitogenic activity on glial cells. We examined whether glial secretion of the dopaminergic growth factor GDNF is responsible for the trophic activity of bFGF on mesencephalic DA neurons. When added with lower concentrations of bFGF, GDN...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/0006-8993(96)00311-3

    authors: Hou JG,Mytilineou C

    更新日期:1996-06-10 00:00:00

  • Involvement of hypothalamic noradrenergic systems in the modulation of intestinal motility in rats.

    abstract::Selective lesions of the noradrenergic systems of the paraventricular nucleus (PVN) of the hypothalamus with 6-hydroxydopamine (6-OHDA) lengthen the periodicity of the migrating myoelectric complex (MMC), an index of intestinal motility, in rats. These lengthening effects resemble those obtained after lesions of the l...

    journal_title:Brain research

    pub_type: 杂志文章

    doi:10.1016/s0006-8993(10)80045-9

    authors: Bonaz B,Martin L,Beurriand E,Hostein J,Feuerstein C

    更新日期:1992-06-26 00:00:00