Abstract:
:The polyamines spermidine and spermine, and their precursor putrescine, have been shown to play an important role in cell migration, proliferation, and differentiation. Because of their polycationic property, polyamines are traditionally thought to be involved in DNA replication, gene expression, and protein translation. However, polyamines can also be covalently conjugated to proteins by transglutaminase 2 (TG2). This modification leads to an increase in positive charge in the polyamine-incorporated region which significantly alters the structure of proteins. It is anticipated that protein polyamine conjugation may affect the protein-protein interaction, protein localization, and protein function of the TG2 substrates. In order to investigate the roles of polyamine modification, we synthesized a spermine-conjugated antigen and generated an antiserum against spermine. In vitro TG2-catalyzed spermine incorporation assays were carried out to show that actin, tubulins, heat shock protein 70 and five types of histone proteins were modified with spermine, and modification sites were also identified by liquid chromatography and linear ion trap-orbitrap hybrid mass spectrometry. Subsequent mass spectrometry-based shotgun proteomic analysis also identified 254 polyaminated sites in 233 proteins from the HeLa cell lysate catalyzed by human TG2 with spermine, thus allowing, for the first time, a global appraisal of site-specific protein polyamination. Global analysis of mouse tissues showed that this modification really exists in vivo. Importantly, we have demonstrated that there is a new histone modification, polyamination, in cells. However, the functional significance of histone polyamination demands further investigations.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Yu CH,Chou CC,Lee YJ,Khoo KH,Chang GDdoi
10.1007/s00726-014-1879-8subject
Has Abstractpub_date
2015-03-01 00:00:00pages
469-81issue
3eissn
0939-4451issn
1438-2199journal_volume
47pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Alterations in adipocyte characteristics are highly implicated in the pathology of obesity. In a recent article, we demonstrated that high-fat diet-induced obesity impairs lysosomal function, thereby suppressing autophagy in mice white adipose tissue. Taurine, an amino acid naturally contained in the normal diet and e...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2550-6
更新日期:2018-05-01 00:00:00
abstract::Taurine (TAU) has been shown to improve exercise time to exhaustion and 3-km running performance; however, no studies have considered the effect of acute TAU ingestion on short duration cycling time trial (TT) performance. The aim of this study was to determine the effects of a single oral acute dose of 1000 mg of TAU...
journal_title:Amino acids
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00726-016-2282-4
更新日期:2016-11-01 00:00:00
abstract::The lysine catabolism pathway differs in adult mammalian brain from that in extracerebral tissues. The saccharopine pathway is the predominant lysine degradative pathway in extracerebral tissues, whereas the pipecolate pathway predominates in adult brain. The two pathways converge at the level of ∆(1)-piperideine-6-ca...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1590-1
更新日期:2013-12-01 00:00:00
abstract::Two novel cyclic hexapeptides, designated NW-G10 (1) and NW-G11 (2), were isolated from the fermentation broth of Streptomyces alboflavus 313. Their relative structures were elucidated on the basis of extensive spectroscopic analysis, and the absolute configurations of several constituent amino acids were determined b...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1303-1
更新日期:2012-11-01 00:00:00
abstract::The study was designed to investigate the effects of NMDA receptor agonist on the behavioral activity in rats with experimental hyperammonemia. The experiments were performed on adult male Wistar rats. Experimental hyperammonemia was induced by intraperitoneal injections of tioacetamide (TAA, 200 mg/kg) for three cons...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-004-0122-4
更新日期:2005-02-01 00:00:00
abstract::The tailor made amino acid β-N-methyl-amino-L-alanine (BMAA) is a neurotoxin produced by cyanobacteria. It has been associated with certain forms of progressive neurodegenerative disease, including sporadic Amyotrophic Lateral Sclerosis and Alzheimer's disease. Some different reports of BMAA in cyanobacterial blooms f...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1057-1
更新日期:2012-06-01 00:00:00
abstract::The specific function of putative cut2 protein (or CFP25), encoded by the Rv2301 gene from Mycobacterium tuberculosis H37Rv, has not been identified yet. The aim of this study was to assess some of CFP25 characteristics and its possible biological role in Mycobacterium tuberculosis H37Rv invasion process to target cel...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0938-7
更新日期:2012-06-01 00:00:00
abstract::Studies have shown that specific amino acids are required for optimal growth of leukemic versus normal cells, and it is believed that the depletion of selected amino acids can abrogate tumor growth. We have developed a technique for studying the effect of amino acid deprivation on leukemic cell proliferation. The tech...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805995
更新日期:1992-10-01 00:00:00
abstract::Arginine is a precursor of proteins and employed in urea synthesis. It is also the precursor of many other compounds, such as creatine, nitric oxide, polyamines, agmatine, proline. In this review, its transport and that of other basic amino acids are examined, along with its transformation into nitric oxide, agmatine ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-004-0081-9
更新日期:2004-07-01 00:00:00
abstract::Animals at the neonatal stage have to eat more to support better growth and health. However, it is difficult to understand the mechanism of feeding during an early stage of life in the brain of the rodent model. Chickens are precocial and they can look for their food by themselves right after hatching. Neonatal chicks...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-019-02762-x
更新日期:2019-08-01 00:00:00
abstract::There is an extensive and still growing body of the literature supporting the efficacy of creatine (Cr) supplementation. In sports, creatine has been recognized as the most effective nutritional supplement in enhancing exercise tolerance, muscle strength and lean body mass. From a clinical perspective, the application...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1132-7
更新日期:2012-08-01 00:00:00
abstract::Dietary polyamines and amino acids (AAs) are crucial for human growth, development, reproduction, and health. However, the scientific literature shows large variations in polyamine and AA concentrations among major staple foods of plant origin, and there is a scarcity of information regarding their complete compositio...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02751-0
更新日期:2019-08-01 00:00:00
abstract::In yeast, toxicity of acetaminophen (APAP), a frequently used analgesic and antipyretic drug, depends on ubiquitin-controlled processes. Previously, we showed a remarkable overlap in toxicity profiles between APAP and tyrosine, and a similarity with drugs like rapamycin and quinine, which induce degradation of the ami...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2613-8
更新日期:2018-10-01 00:00:00
abstract::This study was conducted to evaluate the anti-obesity effects of long-term taurine supplementation in a mild obese ICR mouse model and to study the mechanism by which taurine induces weight loss. Three groups of male ICR mice were fed a normal chow diet, a high-fat diet (HFD), or an HFD supplemented with 2% taurine in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2659-7
更新日期:2019-02-01 00:00:00
abstract::In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0556-6
更新日期:2008-04-01 00:00:00
abstract::Using the olfactory bulbectomy model of depression, we examined the antidepressant-like activity of 2-methyl-6-(phenylethynyl)-pyridine (MPEP) in rats. Bulbectomized rats required a significantly greater number of trials to acquire the response similar to sham-operated controls in the passive avoidance model. Both the...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-001-0131-5
更新日期:2002-01-01 00:00:00
abstract::Among many actions assigned to taurine (Tau), the most abundant amino acid in numerous mammalian tissues, it prevents high-fat diet-induced obesity with increasing resting energy expenditure. To sustain this Tau action, the goal of the present study was to explore the acute effects of Tau on baseline and on adrenaline...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0919-x
更新日期:2012-05-01 00:00:00
abstract::Protooncogenes (PO) play a crucial role for brain biology and pathology. Only the concerted action of protooncogenes enables normal brain development. The reliable and sensitive quantification of brain PO is still holding centre stage in neurobiological research. The aim of our study was therefore the determination of...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01318881
更新日期:1999-01-01 00:00:00
abstract::Contemporary theoretical models used in describing electrostatic properties of amino acids in polypeptides rely usually on atomic point charges. Recently noted defects of such models in reproducing protein folding originate from the inadequate representation of the electrostatic term, in particular inability of atomic...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00808443
更新日期:1994-02-01 00:00:00
abstract::A fast and efficient microwave-assisted solid phase peptide synthesis (MW-SPPS) of a 51mer peptide, the main heparin-binding site (60-110) of human pleiotrophin (hPTN), using 2-chlorotrityl chloride resin (CLTR-Cl) following the 9-fluorenylmethyloxycarbonyl/tert-butyl (Fmoc/tBu) methodology and with the standard N,N'-...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0753-6
更新日期:2011-05-01 00:00:00
abstract::A series of hydroxypyrroloindoline (Hpi) containing dipeptides along with the corresponding monomeric Hpi-α-amino acid (Hpi-2-carboxylate), were prepared by reacting a series of N α-protected-tryptophans in aqueous or biphasic [water/cyclopentyl methyl ether (CPME)] solutions containing Oxone® (potassium peroxymonosul...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2364-3
更新日期:2017-02-01 00:00:00
abstract::Anti-tissue transglutaminase (tTG) antibodies are specifically produced in the small-intestinal mucosa of celiac disease (CD) patients. It is now recognized that these antibodies, acting on cell-surface tTG, may play an active role in CD pathogenesis triggering an intracellular response via the activation of different...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1120-y
更新日期:2013-01-01 00:00:00
abstract::Relapse and metastasis are the main causes of unfavorable outcome in head and neck cancers. Whereas, understanding of the molecular background of these processes is far from being complete. Therefore, in this study we aimed to identify potential biomarker candidates of relapse and metastasis in laryngeal squamous cell...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2201-8
更新日期:2016-06-01 00:00:00
abstract::Since the formulation of the concept of "proteomics" in 1995, a plethora of proteomic technologies have been developed in order to study proteomes of tissues, cells and organelles. The powerful new technologies enabled by proteomic approaches have lead to the application of these methods to an exponentially increasing...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-006-0460-5
更新日期:2007-09-01 00:00:00
abstract:OBJECTIVE:Chemo-therapeutic treatment of glioma patients has minor success. Little is known about mechanisms of a pronounced resistance of gliomas towards actual therapies, yet. ABC-1 belongs to the group of transporters known to be involved in the export of hydrophobic substances and vascular regulation. This study in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0171-3
更新日期:2005-03-01 00:00:00
abstract::Transglutaminases (TGs) are a large family of related and ubiquitous enzymes that catalyze the cross-linking of a glutaminyl residue of a protein/peptide substrate to a lysyl residue of a protein/peptide co-substrate. Considerable and intense progress has been made in the understanding of the chemistry, molecular biol...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-1011-2
更新日期:2012-02-01 00:00:00
abstract::The activity of lysine decarboxylase was studied in 3-day-old soybean (Glycine max (L.) Meer cv. Sakai) seedlings also in relation to light conditions. Lysine decarboxylase activity was mainly localized in the roots and to a lesser extent in the hypocotyls and was detectable in both the soluble and particulate fractio...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0029-6
更新日期:2009-01-01 00:00:00
abstract::Hyperhomocysteinemia is an independent risk factor for cardiovascular disease (ischemic disease, such as stroke and myocardial infarction, and arterial and venous thrombotic events) in the general population. We can assume that the association is causal, based on the example of homocystinuria, and on the evidence put ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-003-0026-8
更新日期:2003-12-01 00:00:00
abstract::Regulated proteolysis plays important roles in cell biology and pathological conditions. A crosstalk exists between apoptosis and the ubiquitin-proteasome system, two pathways responsible for regulated proteolysis executed by different proteases. To investigate whether the apoptotic process also affects the 20S protea...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0575-6
更新日期:2011-07-01 00:00:00
abstract::Phenylketonuria (PKU) is an autosomal recessive genetic disease caused by the defects in the phenylalanine hydroxylase (PAH) gene. Individuals homozygous for defective PAH alleles show elevated levels of systemic phenylalanine and should be under strict dietary control to reduce the risk of neuronal damage associated ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0218-5
更新日期:2005-11-01 00:00:00