Putative occurrence of lysine decarboxylase isoforms in soybean (Glycine max) seedlings.

Abstract:

:The activity of lysine decarboxylase was studied in 3-day-old soybean (Glycine max (L.) Meer cv. Sakai) seedlings also in relation to light conditions. Lysine decarboxylase activity was mainly localized in the roots and to a lesser extent in the hypocotyls and was detectable in both the soluble and particulate fractions. The enzyme activity levels were similar during germination under light and dark conditions. With respect to lysine concentration, the initial decarboxylation rate of the soluble fraction showed a saturating curve. Conversely, the initial decarboxylation rate of the particulate fraction showed a sigmoidal curve. These results could suggest that at least two isoforms of lysine decarboxylase are present in different organs of soybean seedlings. In the root soluble fraction, the suicide inhibitor alpha-difluoromethyl-lysine suppressed the activity of lysine decarboxylase and of ornithine decarboxylase to the same extent, but had no effect on arginine decarboxylase activity.

journal_name

Amino Acids

journal_title

Amino acids

authors

Ohe M,Scoccianti V,Bagni N,Tassoni A,Matsuzaki S

doi

10.1007/s00726-008-0029-6

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

65-70

issue

1

eissn

0939-4451

issn

1438-2199

journal_volume

36

pub_type

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