Abstract:
:Effects exerted by heavy isotope substitution in biopolymers on the functioning of whole organisms have not been investigated. We report on the decrease of permissive temperature of nematodes fed with bacteria containing 5,5-D2-lysine. We synthesized 5,5-dideuterolysine and, taking advantage of lysine being an essential amino acid, showed that C. elegans with modified lysine poorly develop from larvae into fertile adult hermaphrodites. This effect occurs only at high temperature within the permissible range for C. elegans (25 °C) and completely vanishes at 15 °C. The only known metabolic involvement of C5 in lysine is in post-translational modification through lysyl hydoxylases. Indeed, siRNA experiments showed that deficiency of let-268/plod lysyl-hydroxylase/glycosydase further amplifies the isotope effect making it apparent even at 20 °C, whereas control siRNAs as well as another lysyl-hydroxylase (psr-1/jmjdD) siRNA do not. We report for the first time that a site-specific deuteration may strongly affect the development of the whole animal organism especially under the conditions of deficiency of the corresponding enzyme. These findings provide the basis for our ongoing efforts to employ isotope effects for fine tuning of metabolic pathways to mitigate pathological processes.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Korneenko TV,Pestov NB,Hurski AL,Fedarkevich AM,Shmanai VV,Brenna JT,Shchepinov MSdoi
10.1007/s00726-017-2386-5subject
Has Abstractpub_date
2017-05-01 00:00:00pages
887-894issue
5eissn
0939-4451issn
1438-2199pii
10.1007/s00726-017-2386-5journal_volume
49pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::The regulation of the expression of indoleamine 2,3-dioxygenase (IDO) was studied in cloned murine macrophages (MT2) and microglial (N11) cells. Both cell lines express IDO and inducible nitric oxide synthase activity after interferon-gamma (IFN-gamma) stimulation. The regulation of IDO expression appears to differ in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345271
更新日期:1998-01-01 00:00:00
abstract::Selective removal of protecting groups under different cleavage mechanisms could be an asset in peptide synthesis, since it provides the feasibility to incorporate different functional groups in similar reactive centres. However, selective protection/deprotection of orthogonal protecting groups in peptides is still ch...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1696-0
更新日期:2014-05-01 00:00:00
abstract::Schiff bases represent a class of molecules widely studied for their importance in organic and coordination chemistry. Despite the large amount of studies on the chemical and biological properties of the Schiff bases, the different experimental conditions prevent a useful comparison to search for a correlation structu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02816-0
更新日期:2020-03-01 00:00:00
abstract::We present an optimized procedure for the synthesis of (S)-vinylglycine from (S)-methionine. The key step is a solvent free pyrolysis of an intermediate sulfoxide at high temperature. Using our optimized reaction conditions, Cbz-protected vinylglycine was obtained in high yield and with almost no side products. The pr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0460-3
更新日期:2010-07-01 00:00:00
abstract::High-protein diets have been shown to alleviate detrimental effects of high-fat diets and this effect can be partially mimicked by dietary L-leucine supplementation. Here, we aimed to elucidate the early mechanisms and the specificity of leucine effects. We performed a 1-week trial with male C57BL/6 mice fed ad libitu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1363-2
更新日期:2013-02-01 00:00:00
abstract::Hyperargininemia is caused by deficiency of arginase 1, which catalyzes the hydrolysis of L-arginine to urea as the final enzyme in the urea cycle. In contrast to other urea cycle defects, arginase 1 deficiency usually does not cause catastrophic neonatal hyperammonemia but rather presents with progressive neurologica...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-015-2032-z
更新日期:2015-09-01 00:00:00
abstract::The reactions of phosphorus trichloride with various amino acids afford the pentacoordinated spirophosphoranes. The reaction procedures were traced by (31)P NMR spectra techniques. A new crystal structure of alanine derivative was characterized, which is a slightly distorted TBP structure. Besides, this kind of spirop...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0207-8
更新日期:2005-06-01 00:00:00
abstract::Extracellular peptide ligand binding sites, which bind the N-termini of angiotensin II (AngII) and bradykinin (BK) peptides, are located on the N-terminal and extracellular loop 3 regions of the AT(1)R and BKRB(1) or BKRB(2) G-protein-coupled receptors (GPCRs). Here we synthesized peptides P15 and P13 corresponding to...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1405-9
更新日期:2013-03-01 00:00:00
abstract::To mutant ddY/DAO(-) mice lacking D-amino-acid oxidase activity and normal ddY/DAO(+) mice, five D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu and D-Pro) were orally administered for two weeks, and the D-amino acid levels were examined in seven brain regions. The levels of D-Asp markedly increased in the pituitary and pin...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0357-8
更新日期:2007-01-01 00:00:00
abstract::Several descriptors of protein structure at the sequence and residue levels have been recently proposed. They are widely adopted in the analysis and prediction of structural and functional characteristics of proteins. Numerous in silico methods have been developed for sequence-based prediction of these descriptors. Ho...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0721-1
更新日期:2011-03-01 00:00:00
abstract::This work is an extension of our previous work (Hall et al., 1993) on the synthesis and cytotoxic activity of boronated peptides. The aim of this work was to carry out structural modifications of the amine terminal in compounds1 and2, to increase water solubility, and its effect on the cytotoxicity to tumor cell lines...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806550
更新日期:1995-12-01 00:00:00
abstract::Sensitivity to pharmacological challenges has been reported in patients with panic disorder. We have previously validated transgenic mice overexpressing the neurotrophin-3 (NT-3) receptor, TrkC (TgNTRK3), as an engineered murine model of panic disorder. We could determine that TgNTRK3 mice presented increased cellular...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0464-1
更新日期:2007-11-01 00:00:00
abstract::The coupling between G protein-coupled receptors (GPCRs) and guanine nucleotide-binding proteins (G proteins) regulates various signal transductions from extracellular space into the cell. However, the coupling mechanism between GPCRs and G proteins is still unknown, and experimental determination of their coupling sp...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1133-6
更新日期:2012-08-01 00:00:00
abstract::Lamin A/C proteins are the major components of a thin proteinaceous filamentous meshwork, the lamina, that underlies the inner nuclear membrane. A few specific mutations in the lamin A/C gene cause a disease with remarkably different clinical features: FPLD, or familial partial lipodystrophy (Dunnigan-type), which mai...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1108-7
更新日期:2012-08-01 00:00:00
abstract::We report the solid phase synthesis and some pharmacological properties of 24 oxytocin (OT) analogues. Basic modifications at position 9 (introduction of L- or D-beta-(2-thienyl)-alanine [L- or D-Thi], or L- or D-3-Pyridylalanine [L- or D-3-Pal]) were combined with D-tyrosine(OEthyl) [D-Tyr(Et)] or D-1-naphthylalanine...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0372-2
更新日期:2010-05-01 00:00:00
abstract::O-linked β-N-actylglucosamine (O-GlcNAc) is a carbohydrate post-translational modification on hydroxyl groups of serine and/or threonine residues of cytosolic and nuclear proteins. Analogous to phosphorylation, O-GlcNAcylation plays crucial regulatory roles in a variety of cellular processes. O-GlcNAc was termed a nut...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1543-8
更新日期:2013-10-01 00:00:00
abstract::Ocular ischemia resulting from perfusion disturbances may play a major role in initiation of glaucoma. Possibly secondary to ischemia autoimmunogenic events are activated in glaucoma patients with increased prevalence of systemic autoimmune diseases. The determination of potential molecular markers in blood leukocytes...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-001-0141-3
更新日期:2002-01-01 00:00:00
abstract::Green tea which is widely consumed in China, Japan and India, contains polyphenolic compounds, which account for 30% of the dry weight of the leaves. Most of the polyphenols are flavanols, of which (-)-epigallocatechin-3-gallate (EGCG) is most abundant. Epidemiological studies revealed that the incidences of stomach a...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s726-002-8197-9
更新日期:2002-01-01 00:00:00
abstract::The lens transparency depends on higher concentration of lens proteins and their interactions. α-Crystallin is one of the predominant lens proteins, responsible for proper structural and functional architecture of the lens microenvironment, and any alteration of which results in cataract formation. The R12C, R21L, R49...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1935-z
更新日期:2015-05-01 00:00:00
abstract::Two synthetic routes to bis-armed-alpha-amino acid derivatives are described. The first route involves alkylation of dibromo derivatives with ethyl isocyanoacetate under phase-transfer catalysis (PTC) conditions. The second route uses a palladium-mediated Suzuki-Miyaura cross-coupling reaction between a DL-4-boronophe...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0402-2
更新日期:2007-01-01 00:00:00
abstract::Chiral dehydroamino acid building blocks are versatile starting materials for the preparation of optically active unusual amino acids and other compounds of pharmacological interest. Herein we disclose the expedient preparation of dehydroalanines (ΔAla) equipped with oxazolidin-2-one (Oxd) chiral auxiliaries, Ts-Oxd-Δ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1839-3
更新日期:2014-12-01 00:00:00
abstract::Astrocytes are glial cells in the central nervous system (CNS) that play key roles in brain physiology, controlling processes, such as neurogenesis, brain energy metabolism and synaptic transmission. Recently, immune functions have also been demonstrated in astrocytes, influencing neuronal survival in the course of ne...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0691-3
更新日期:2011-02-01 00:00:00
abstract::The effects of Red Bull Energy Drink, which includes taurine, glucuronolactone, and caffeine amongst the ingredients, were examined over 3 studies in a total of 36 volunteers. Assessments included psychomotor performance (reaction time, concentration, memory), subjective alertness and physical endurance. When compared...
journal_title:Amino acids
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/s007260170021
更新日期:2001-01-01 00:00:00
abstract::The expression of pluripotent stem cell protein markers, self-renewal, the potential to differentiate in cell types of all three germlines and teratoma formation in nude mice form the spectrum of the stringent pluripotency criteria for human stem cells. Currently, intercellular variability is discussed as an additiona...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-013-1599-5
更新日期:2013-12-01 00:00:00
abstract::Dopa and related catecholamines and their degradation products have been demonstrated to have neurotoxic potential in a number of cellular and in vivo experiments. Several mechanisms have been hypothesized to be involved including generation of prooxidant products that subsequently oxidize membrane lipids and exposed ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345261
更新日期:1998-01-01 00:00:00
abstract::Ornithine decarboxylase antizyme 1 (AZ1) is a major regulatory protein responsible for the regulation and degradation of ornithine decarboxylase (ODC). To better understand the role of AZ1 in polyamine metabolism and in modulating the response to anticancer polyamine analogues, a small interfering RNA strategy was use...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1030-z
更新日期:2012-02-01 00:00:00
abstract::Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time exposure of UV irradiation decreases procollagen mRNA expression in dermis and that chronic UV irradiation decreases collagen amounts and induces wrinkle formation. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1059-z
更新日期:2012-06-01 00:00:00
abstract::Amnesia is a deficit in memory caused by brain damage, disease, or trauma. Until now, there are no successful medications on the drug market available to treat amnesia. Short analogs and mimetics of human urocortin 3 (Ucn 3) tripeptide were synthetized and tested for their action against amnesia induced by eletroconvu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2268-2
更新日期:2016-09-01 00:00:00
abstract::Using the olfactory bulbectomy model of depression, we examined the antidepressant-like activity of 2-methyl-6-(phenylethynyl)-pyridine (MPEP) in rats. Bulbectomized rats required a significantly greater number of trials to acquire the response similar to sham-operated controls in the passive avoidance model. Both the...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-001-0131-5
更新日期:2002-01-01 00:00:00
abstract::α-Pharmacological conotoxins are among the most selective ligands of nicotinic acetylcholine receptors with typical cysteine frameworks. They are characterized by the intercysteine loop and classified into various subfamilies, such as α3/5 and α4/7 conotoxins. A novel α-conotoxin, Pu14a (DCPPHPVPGMHKCVCLKTC), with a d...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1093-x
更新日期:2012-07-01 00:00:00