Alterations in D-amino acid levels in the brains of mice and rats after the administration of D-amino acids.


:To mutant ddY/DAO(-) mice lacking D-amino-acid oxidase activity and normal ddY/DAO(+) mice, five D-amino acids (D-Asp, D-Ser, D-Ala, D-Leu and D-Pro) were orally administered for two weeks, and the D-amino acid levels were examined in seven brain regions. The levels of D-Asp markedly increased in the pituitary and pineal glands in both strains. In the ddY/DAO(+) mice, the levels of the other D-amino acids did not significantly change in most of the brain regions. While in the ddY/DAO(-) mice the levels of D-Ser significantly increased in most of the brain regions except for the cerebrum and hippocampus. The levels of D-Ala and D-Leu increased in all regions but the levels of D-Pro did not significantly change. The same five D-amino acids were intravenously injected into Wistar rats and the D-amino acid levels in their brains were examined for 60 min after the administration. The levels of D-Asp markedly increased in the pineal gland 3 min after the administration, while the levels of D-Ser, D-Ala, and D-Pro increased both in the pineal and pituitary glands, the levels of D-Leu increased in all brain regions. These results are useful for the elucidation of the origins and regulation of D-amino acids in the mammalian body.


Amino Acids


Amino acids


Morikawa A,Hamase K,Inoue T,Konno R,Zaitsu K




Has Abstract


2007-01-01 00:00:00












  • Production of hypotaurine, taurine and sulfate in rats and mice injected with L-cysteinesulfinate.

    abstract::We studied in vivo production of taurine, hypotaurine and sulfate following subcutaneous administration of L-cysteinesulfinate (CSA) to rats and mice. When 5.0 mmol/kg of body weight of CSA was injected to rats, increased urinary excretions of taurine, hypotaurine and sulfate in 24 h urine were 617, 52 and 1,767 micro...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Nakamura H,Yatsuki J,Ubuka T

    更新日期:2006-07-01 00:00:00

  • Two piperazic acid-containing cyclic hexapeptides from Streptomyces alboflavus 313.

    abstract::Two novel cyclic hexapeptides, designated NW-G10 (1) and NW-G11 (2), were isolated from the fermentation broth of Streptomyces alboflavus 313. Their relative structures were elucidated on the basis of extensive spectroscopic analysis, and the absolute configurations of several constituent amino acids were determined b...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Wei S,Fan L,Wu W,Ji Z

    更新日期:2012-11-01 00:00:00

  • Differential protein expression profile in gastrointestinal stromal tumors.

    abstract::Gastrointestinal stromal tumors (GISTs) arise from the interstitial cells of Cajal through gain of function mutations of the oncogene KIT. Imatinib offers the first effective treatment for patients with GISTs, but the therapeutic outcome strongly depends on the type of KIT mutation. We used ProteinChip technology to i...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Landuyt B,Prenen H,Debiec-Rychter M,Sciot R,de Bruijn EA,van Oosterom AT

    更新日期:2004-12-01 00:00:00

  • Pharmacological approaches to counter the toxicity of Dopa.

    abstract::Dopa and related catecholamines and their degradation products have been demonstrated to have neurotoxic potential in a number of cellular and in vivo experiments. Several mechanisms have been hypothesized to be involved including generation of prooxidant products that subsequently oxidize membrane lipids and exposed ...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: VonVoigtlander PF,Fici GJ,Althaus JS

    更新日期:1998-01-01 00:00:00

  • Differential associations of circulating asymmetric dimethylarginine and cell adhesion molecules with metformin use in patients with type 2 diabetes mellitus and stable coronary artery disease.

    abstract::Metformin, the drug of first choice in type 2 diabetes mellitus (T2DM), reduces cardiovascular (CV) morbidity and mortality in part independently of improved glycemic control and changes in traditional risk factors. However, there are discordant reports on the effects of metformin on endothelial function in T2DM. Our ...

    journal_title:Amino acids

    pub_type: 临床试验,杂志文章


    authors: Kruszelnicka O,Chyrchel B,Golay A,Surdacki A

    更新日期:2015-09-01 00:00:00

  • Oxygen radical induced fluorescence in proteins; identification of the fluorescent tryptophan metabolite, N-formyl kynurenine, as a biological index of radical damage.

    abstract::The effect of oxygen derived free radicals (OFR) on aromatic and sulphur containing amino acids has been investigated, both in their free form and within protein backbones. Aerated amino acids and proteins in solution were exposed to three discrete OFR generating systems; (1) gamma radiation in the presence or absence...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Griffiths HR,Lunec J,Blake DR

    更新日期:1992-06-01 00:00:00

  • Synthesis and biological activity of oxytocin analogues containing unnatural amino acids in position 9: structure activity study.

    abstract::We report the solid phase synthesis and some pharmacological properties of 24 oxytocin (OT) analogues. Basic modifications at position 9 (introduction of L- or D-beta-(2-thienyl)-alanine [L- or D-Thi], or L- or D-3-Pyridylalanine [L- or D-3-Pal]) were combined with D-tyrosine(OEthyl) [D-Tyr(Et)] or D-1-naphthylalanine...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Magafa V,Borovicková L,Slaninová J,Cordopatis P

    更新日期:2010-05-01 00:00:00

  • Ketimine reductase/CRYM catalyzes reductive alkylamination of α-keto acids, confirming its function as an imine reductase.

    abstract::Recently, crystalized mouse ketimine reductase/CRYM complexed with NADPH was found to have pyruvate bound in its active site. We demonstrate that the enzyme binds α-keto acids, such as pyruvate, in solution, and catalyzes the formation of N-alkyl-amino acids from alkylamines and α-keto acids (via reduction of imine in...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Hallen A,Cooper AJ,Smith JR,Jamie JF,Karuso P

    更新日期:2015-11-01 00:00:00

  • D-Aspartyl residue in a peptide can be liberated and metabolized by pig kidney enzymes.

    abstract::The presence of an enzyme activity which hydrolyzes glycyl-D-aspartate was found in the homogenates of pig kidney cortex. The activity was inhibited by metal chelating agents and cilastatin, suggesting that the enzyme was a cilastatin-sensitive metallo-peptidase. Of the two hydrolysis products,D-aspartate was found to...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Kera Y,Funabashi K,Matsumoto T,Watanabe T,Nagasaki H,Yamada R

    更新日期:1996-06-01 00:00:00

  • Design, synthesis and biological evaluation of non-peptide PAR1 thrombin receptor antagonists based on small bifunctional templates: arginine and phenylalanine side chain groups are keys for receptor activity.

    abstract::In the present study, we report the synthesis and biological evaluation of a series of new non-peptide PAR(1) mimetic receptor antagonists, based on conformational analysis of the S(42)FLLR(46) tethered ligand (TL) sequence of PAR(1). These compounds incorporate the key pharmacophore groups in the TL sequence, guanidy...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Androutsou ME,Saifeddine M,Hollenberg MD,Matsoukas J,Agelis G

    更新日期:2010-04-01 00:00:00

  • Coenzyme preference of Streptococcus pyogenes δ1-pyrroline-5-carboxylate reductase: evidence supporting NADPH as the physiological electron donor.

    abstract::The streptococcal enzyme that catalyzes the last step in proline biosynthesis was heterologously expressed and the recombinant protein was purified to electrophoretic homogeneity and characterized thoroughly. As for δ1-pyrroline-5-carboxylate reductases from other sources, it was able to use either NADH or NADPH as th...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Petrollino D,Forlani G

    更新日期:2012-07-01 00:00:00

  • The effect of tryptophan administration on ileum contractility and oxidant status in mice.

    abstract::L-tryptophan (TRP) is the precursor amino acid for the synthesis of serotonin (5-HT). 5-HT is effective both on the food intake and gastrointestinal system contractility. The aim of this study was to search the effects of systemic TRP treatment on 5-HT levels of ileum and searching the effect of ileal contractility an...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Ozer C,Gönül B,Ercan ZS,Take G,Erdoğan D

    更新日期:2007-01-01 00:00:00

  • Effects of dietary L-arginine or N-carbamylglutamate supplementation during late gestation of sows on the miR-15b/16, miR-221/222, VEGFA and eNOS expression in umbilical vein.

    abstract::Placental vascular formation and blood flow are crucial for fetal survival, growth and development, and arginine regulates vascular development and function. This study determined the effects of dietary arginine or N-carbamylglutamate (NCG) supplementation during late gestation of sows on the microRNAs, vascular endot...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Liu XD,Wu X,Yin YL,Liu YQ,Geng MM,Yang HS,Blachier F,Wu GY

    更新日期:2012-06-01 00:00:00

  • Abomasal infusion of arginine stimulates SCD and C/EBPß gene expression, and decreases CPT1ß gene expression in bovine adipose tissue independent of conjugated linoleic acid.

    abstract::Based on previous research with bovine peadipocytes, we hypothesized that infusion of arginine into the abomasum of Angus steers stimulates stearoyl-CoA desaturase (SCD) gene expression in bovine subcutaneous (s.c.) adipose tissue, and that this would be attenuated by conjugated linoleic acid (CLA). Growing Angus stee...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Choi SH,Wickersham TA,Wu G,Gilmore LA,Edwards HD,Park SK,Kim KH,Smith SB

    更新日期:2014-02-01 00:00:00

  • Asymmetric synthesis of quaternary α-amino acid derivatives and their fluorinated analogues.

    abstract::In this work, we describe the asymmetric synthesis of a series of fluorinated and non-fluorinated quaternary α-amino acid derivatives. This methodology involves the diastereoselective addition of chiral 2-p-tolylsulfinyl benzylcarbanions to either imines containing a 2-furyl moiety or trifluoromethyl α-imino esters. S...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Fustero S,Sánchez-Roselló M,Báez C,Del Pozo C,García Ruano JL,Alemán J,Marzo L,Parra A

    更新日期:2011-08-01 00:00:00

  • Isolation and antiproliferation of tumor cells by a novel peptide (TC22) from the beetle Tribolium castaneum.

    abstract::Anticancer peptides (ACPs) are biologically anticancer active molecules that are produced by mammals, plants, insects and microorganisms. Here, a new peptide (TC22) with the amino acid sequence MTVVLLLIVLPLLGGVHSSGIL was identified and characterized from the beetle Tribolium castaneum. We found it inhibited the growth...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Liu XD,Zhang FX,Qin ZH,Shan H

    更新日期:2019-02-01 00:00:00

  • Selective determination of mimosine and its dihydroxypyridinyl derivative in plant systems.

    abstract::Our observations on the growth stimulatory nature of mimosine, (beta-(3-hydroxy-4-pyridon-1-yl)-L-alanine), the toxic non-protein plant amino acid, in some model experimental systems, warranted sensitive and selective routine estimations. For the determination of both mimosine and DHP, an indirect spectrophotometric m...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Lalitha K,Rajendra Kulothungan S

    更新日期:2006-10-01 00:00:00

  • An improved protocol for the preparation of (S)-vinylglycine from (S)-methionine.

    abstract::We present an optimized procedure for the synthesis of (S)-vinylglycine from (S)-methionine. The key step is a solvent free pyrolysis of an intermediate sulfoxide at high temperature. Using our optimized reaction conditions, Cbz-protected vinylglycine was obtained in high yield and with almost no side products. The pr...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Küchenthal CH,Migenda J,Polednia M,Maison W

    更新日期:2010-07-01 00:00:00

  • Evolutionary relationships between seryl-histidine dipeptide and modern serine proteases from the analysis based on mass spectrometry and bioinformatics.

    abstract::Seryl-histidine dipeptide (Ser-His) has been recognized as the shortest peptide with hydrolysis cleavage activity; however, its protein cleavage spectrum has not yet been fully explored. Here, four differently folded proteins were treated with Ser-His, and the digestion products were evaluated with high-resolution mas...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Liu Y,Li YB,Gao X,Yu YF,Liu XX,Ji ZL,Ma Y,Li YM,Zhao YF

    更新日期:2018-01-01 00:00:00

  • Leucine supplementation does not affect protein turnover and impairs the beneficial effects of endurance training on glucose homeostasis in healthy mice.

    abstract::Endurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. ...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Costa Júnior JM,Rosa MR,Protzek AO,de Paula FM,Ferreira SM,Rezende LF,Vanzela EC,Zoppi CC,Silveira LR,Kettelhut IC,Boschero AC,de Oliveira CA,Carneiro EM

    更新日期:2015-04-01 00:00:00

  • Evidence that the tri-cellular metabolism of N-acetylaspartate functions as the brain's "operating system": how NAA metabolism supports meaningful intercellular frequency-encoded communications.

    abstract::N-acetylaspartate (NAA), an acetylated derivative of L-aspartate (Asp), and N-acetylaspartylglutamate (NAAG), a derivative of NAA and L-glutamate (Glu), are synthesized by neurons in brain. However, neurons cannot catabolize either of these substances, and so their metabolism requires the participation of two other ce...

    journal_title:Amino acids

    pub_type: 杂志文章,评审


    authors: Baslow MH

    更新日期:2010-11-01 00:00:00

  • Observations on the relationship between the extracellular changes of taurine and glutamate during cortical spreading depression, during ischemia, and within the area surrounding a thrombotic infarct.

    abstract::Taurine and glutamate were monitored by microdialysis technique during various cerebral insults: a. Application of K+ triggered a cortical spreading depression (CSD). Taurine and glutamate increased concomitantly but recovery of glutamate was faster than that of taurine. b. Application of NMDA induced also CSD but onl...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Scheller D,Szathmary S,Kolb J,Tegtmeier F

    更新日期:2000-01-01 00:00:00

  • Disturbance of erythrocyte lipid bilayer by amino acid-based surfactants.

    abstract::In an attempt to increase our knowledge regarding the mechanisms of surfactant membrane interaction, we studied the action of several anionic and cationic amino acid-based surfactants on membrane fluidity using fluorescence anisotropy. Anisotropy measurements demonstrated that almost all of the surfactants studied dis...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Martínez V,Sánchez L,Busquets MA,Infante MR,Pilar Vinardell M,Mitjans M

    更新日期:2007-09-01 00:00:00

  • Cysteine homeostasis under inhibition of protein synthesis in Escherichia coli cells.

    abstract::Increased intracellular cysteine poses a potential danger to cells due to the high ability of cysteine to reduce free iron and promote the Fenton reaction. Here, we studied ways to maintain cysteine homeostasis in E. coli cells while inhibiting protein synthesis with valine or chloramphenicol. When growing wild-type b...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Smirnova GV,Tyulenev AV,Bezmaternykh KV,Muzyka NG,Ushakov VY,Oktyabrsky ON

    更新日期:2019-11-01 00:00:00

  • PET imaging of Hsp90 expression in pancreatic cancer using a new 64Cu-labeled dimeric Sansalvamide A decapeptide.

    abstract::Heat shock protein 90 (Hsp90) plays a vital role in the progress of malignant disease and elevated Hsp90 expression has been reported in pancreatic cancer. In this study, we radiolabeled a dimeric Sansalvamide A derivative (Di-San A1) with 64Cu, and evaluated the feasibility of using 64Cu-Di-San A1 for PET imaging of ...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Wang X,Zhang J,Wu H,Li Y,Conti PS,Chen K

    更新日期:2018-07-01 00:00:00

  • Tryptophanase-catalysed degradation of D-tryptophan in highly concentrated diammonium hydrogen phosphate solution.

    abstract::Tryptophanase is and is perfectly inert to D-tryptophan under ordinary conditions. However, activity that can degrade D-tryptophan into indole is observed when tryptophanase is in highly concentrated diammoniumhydrogen phosphate solution. The reaction has been so far unknown in tryptophanase metabolic pathways. Here w...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Shimada A,Shishido H,Nakamura I

    更新日期:1996-03-01 00:00:00

  • Loss ofHindIII cleavage sites in theD-amino acid oxidase gene in some inbred strains of mice.

    abstract::D-Amino acid oxidase cDNA was amplified by a polymerase chain reaction using RNA extracted from the mouse kidney. When digested withHindIII, the cDNAs of the BALB/c and ddY/DAO(-) mice were cleaved into two fragments whereas the cDNA of the ddY/DAO(+) mice was not. Sequencing revealed that nucleotide-471 of the cDNAs ...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Konno R,Sasaki M,Enami J,Niwa A

    更新日期:1995-03-01 00:00:00

  • Knockdown of ornithine decarboxylase antizyme 1 causes loss of uptake regulation leading to increased N1, N11-bis(ethyl)norspermine (BENSpm) accumulation and toxicity in NCI H157 lung cancer cells.

    abstract::Ornithine decarboxylase antizyme 1 (AZ1) is a major regulatory protein responsible for the regulation and degradation of ornithine decarboxylase (ODC). To better understand the role of AZ1 in polyamine metabolism and in modulating the response to anticancer polyamine analogues, a small interfering RNA strategy was use...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Fraser AV,Goodwin AC,Hacker-Prietz A,Sugar E,Woster PM,Casero RA Jr

    更新日期:2012-02-01 00:00:00

  • Synthesis of N-quinonyltaurines.

    abstract::Both 1,4-benzoquinones and 1,4-naphthoquinones were attached to the non-proteinogenic amino acid taurine to form N-quinonyl taurine derivatives. The products were formed via the direct Michael-like addition or by substitution of a good leaving group. An attempt to bridge the two moieties via an ureido spacer resulted ...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: Gorohovsky S,Temtsin-Krayz G,Bittner S

    更新日期:2003-04-01 00:00:00

  • Volatile constituents of glutathione--ribose model system and its antioxidant activity.

    abstract::Reaction between glutathione and ribose was carried out to study the volatiles formed via Maillard reaction and their antioxidant activity as well as their role in inhibition of LDL oxidation. The simultaneous distillation-extraction technique was used for trapping the volatile components followed by GC-MS analysis. T...

    journal_title:Amino acids

    pub_type: 杂志文章


    authors: El-massry K,Farouk A,El-Ghorab A

    更新日期:2003-01-01 00:00:00