Abstract:
OBJECTIVE:Chemo-therapeutic treatment of glioma patients has minor success. Little is known about mechanisms of a pronounced resistance of gliomas towards actual therapies, yet. ABC-1 belongs to the group of transporters known to be involved in the export of hydrophobic substances and vascular regulation. This study investigates an effect of both temozolomide (TMZ) treatment and/or irradiation on the expression of the ABC-1 transporter in human U87-MG glioma cells. MATERIAL AND METHODS:In parallel experiments U87-MG cells underwent either irradiation (RT), chemo-treatment (CT) using TMZ, and combined chemo/radiation-treatment (CT/RT). After each treatment the cells were incubated either 2 or 24 hours at 37 degrees C and counted before protein analysis using Western-Blot technique. RESULTS AND CONCLUSIONS:An exponential growth of cellular density was observed for both untreated and irradiated cells being, however, about 2-times slower in irradiated compared to untreated cells. In contrast the density increase of chemo-treated cells as well as that of cells, which underwent the combined CT/RT treatment was of linear nature. ABC-1 expression was detected in untreated as well as treated cells. Increasing cell density and all kinds of treatment resulted in a considerably enhanced ABC-1 expression. CT treatment resulted in highly up-regulated ABC-1 expression especially in non-confluent cultures compared to untreated cells. Irradiation had a comparable or even higher inducible effect on the ABC-1 expression rates depending, however, on cell density. The highest expression rates were observed in cultures with high cellular density 2 hours after application of the combined treatment. Strong up-regulation of ABC-1 expression under both irradiation and chemo-treatment might be a clue to multidrug and irradiation cross-resistance mechanisms of malignant glioma cells converting the ABC-1 transporter into an attractive pharmacological target for a clinical breakthrough in the therapy of malignant gliomas.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Trog D,Moenkemann H,Haertel N,Schüller H,Golubnitschaja Odoi
10.1007/s00726-005-0171-3subject
Has Abstractpub_date
2005-03-01 00:00:00pages
213-9issue
2eissn
0939-4451issn
1438-2199journal_volume
28pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::One pathogenic mechanism of ethanol-induced liver injury is the excessive production of reactive oxygen species (ROS), which may result in alcoholic liver disease (ALD) characterized by cell death due to necrosis and apoptosis. Taurine was proved to protect against liver damage. However, whether taurine attenuates eth...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2561-3
更新日期:2018-07-01 00:00:00
abstract::We recently reported that dietary supplementation with L-proline (proline) during gestation improved embryonic survival in C57BL/6J mice. The objective of the present study was to test the hypothesis that the effect of maternal proline supplementation on embryonic survival can be carried forward to the first generatio...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02717-2
更新日期:2019-05-01 00:00:00
abstract::The enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as "structure breaking" solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-017-2499-x
更新日期:2018-01-01 00:00:00
abstract::The transient receptor potential channels TRPV2 and TRPV5 belong to the vanilloid TRP subfamily. TRPV2 is highly similar to TRPV1 and shares many common properties with it. TRPV5 (and also its homolog TRPV6) is a rather distinct member of the TRPV subfamily. It is distant for being strictly Ca(2+)-selective and featur...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0712-2
更新日期:2011-02-01 00:00:00
abstract::Starting from a chiral 4-hydroxymethyl pyrrolidin-2-one, an isostere of α-methyl homoserine tethered on a γ-lactam ring was prepared exploiting a stereoselective acylation-methylation sequence, followed by Curtius rearrangement, and structural assignment was confirmed by n.O.e. experiments. By reverting the sequence, ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1671-9
更新日期:2014-04-01 00:00:00
abstract::We report the solid phase synthesis and some pharmacological properties of 24 oxytocin (OT) analogues. Basic modifications at position 9 (introduction of L- or D-beta-(2-thienyl)-alanine [L- or D-Thi], or L- or D-3-Pyridylalanine [L- or D-3-Pal]) were combined with D-tyrosine(OEthyl) [D-Tyr(Et)] or D-1-naphthylalanine...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0372-2
更新日期:2010-05-01 00:00:00
abstract::Gamma-aminobutyric acid (GABA) biosynthesis depended to a great extent on the biotransformation characterization of glutamate decarboxylase (GAD) and process conditions. In this paper, the enhancing effect of D101 macroporous adsorption resin (MAR) on the GABA production was investigated based on the whole-cell biotra...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-020-02850-3
更新日期:2020-05-01 00:00:00
abstract::Short CCK peptides elicit panic attacks in humans and anxiogenic-like effects in some animal models, but CCK receptor antagonists have not been found clinically effective. Yet CCK overactivity appears to be involved in submissive behaviour, and CCKB receptor expression and binding are increased in suicide victims and ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-006-0334-x
更新日期:2006-10-01 00:00:00
abstract::We studied in vivo production of taurine, hypotaurine and sulfate following subcutaneous administration of L-cysteinesulfinate (CSA) to rats and mice. When 5.0 mmol/kg of body weight of CSA was injected to rats, increased urinary excretions of taurine, hypotaurine and sulfate in 24 h urine were 617, 52 and 1,767 micro...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-005-0277-7
更新日期:2006-07-01 00:00:00
abstract::Studies have shown that specific amino acids are required for optimal growth of leukemic versus normal cells, and it is believed that the depletion of selected amino acids can abrogate tumor growth. We have developed a technique for studying the effect of amino acid deprivation on leukemic cell proliferation. The tech...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00805995
更新日期:1992-10-01 00:00:00
abstract::Muscle carnosine synthesis is limited by the availability of beta-alanine. Thirteen male subjects were supplemented with beta-alanine (CarnoSyn) for 4 wks, 8 of these for 10 wks. A biopsy of the vastus lateralis was obtained from 6 of the 8 at 0, 4 and 10 wks. Subjects undertook a cycle capacity test to determine tota...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-006-0364-4
更新日期:2007-02-01 00:00:00
abstract::Pyridoxamine (PM) has long been known to inhibit protein glycation via various mechanisms of action. One such mechanism involves the scavenging of carbonyl compounds with glycating ability. Despite the abundant literature on this topic, few quantitative kinetic studies on the processes involved have been reported. In ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0098-6
更新日期:2009-03-01 00:00:00
abstract::A fast and efficient microwave-assisted solid phase peptide synthesis (MW-SPPS) of a 51mer peptide, the main heparin-binding site (60-110) of human pleiotrophin (hPTN), using 2-chlorotrityl chloride resin (CLTR-Cl) following the 9-fluorenylmethyloxycarbonyl/tert-butyl (Fmoc/tBu) methodology and with the standard N,N'-...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0753-6
更新日期:2011-05-01 00:00:00
abstract::The kidney plays a key role in arginine metabolism. Arginine production is controlled by argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) which metabolize citrulline and aspartate to arginine and fumarate whereas arginine consumption is dependent on arginine:glycine amidinotransferase (GAT), which ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0897-z
更新日期:2012-04-01 00:00:00
abstract::This study determined whether N-acetylcysteine (NAC) could affect intestinal redox status, proinflammatory cytokines, epidermal growth factor (EGF), EGF receptor (EGFR), Toll-like receptor-4 (TLR4), and aquaporin-8 in a lipopolysaccharide (LPS)-challenged piglet model. Eighteen piglets (35-day-old) were randomly alloc...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1295-x
更新日期:2013-09-01 00:00:00
abstract::Agmatine, a metabolite generated by arginine decarboxylation, has been reported as neuromodulator and neuroactive substance. Several findings suggest that agmatine displays neuroprotective effects in several models of neurodegenerative disorders, such as Parkinson's disease (PD). It has been hypothesized that biogenic...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02759-6
更新日期:2020-02-01 00:00:00
abstract::Amino acid networks (AANs) are undirected networks consisting of amino acid residues and their interactions in three-dimensional protein structures. The analysis of AANs provides novel insight into protein science, and several common amino acid network properties have revealed diverse classes of proteins. In this revi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-014-1710-6
更新日期:2014-06-01 00:00:00
abstract::The purpose of this study was to investigate H(e) concentration in plasma from 80 healthy donors in relation to age (6 newborns are also included), sex, daily variation (9, 11 a.m.; 2, 6, 12 p.m.) and a period of 5 subsequent months. A significant correlation (r = 0.63, p < 0.001) was observed between plasma H(e) and ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00806188
更新日期:1993-02-01 00:00:00
abstract::Hyperhomocysteinemia is an independent risk factor for cardiovascular disease (ischemic disease, such as stroke and myocardial infarction, and arterial and venous thrombotic events) in the general population. We can assume that the association is causal, based on the example of homocystinuria, and on the evidence put ...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-003-0026-8
更新日期:2003-12-01 00:00:00
abstract::The role of post-translational modification of cell proteins with polyamines, a reaction catalyzed by a tissue tranglutaminase (TG, EC 2.3.2.13), in the induction of cell differentiation, represents an intriguing strategy to control cell proliferation and metastatic ability of different tumor cell lines. In this revie...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-008-0157-z
更新日期:2009-04-01 00:00:00
abstract::For medical use of proteins and peptide-based drugs, it is desirable to have small biologically active sequences because they improve stability, reduce side effects, and production costs. Several plant defensins have their biological activities imparted by a sequence named γ-core. Vu-Def, a Vigna unguiculata defensin,...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02800-8
更新日期:2019-11-01 00:00:00
abstract::The effects of dietary taurine on the experimental colitis induced by dextran sulfate sodium (DSS) in mice were evaluated. C57BL/6 female mice were given 3% DSS in drinking water for 5 d to induce acute colitis. Taurine at 2% was added to the drinking water 5 d before and during the DSS-treatment to investigate its pr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0562-8
更新日期:2008-06-01 00:00:00
abstract::Minerals more readily adsorb amino acids with charged R groups than uncharged R groups, so that the incorporation of amino acids with charged R groups into peptides would be more frequent than for amino acids with uncharged R groups. However, 74% of the amino acids in the proteins of modern organisms contain uncharged...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-004-0106-4
更新日期:2004-08-01 00:00:00
abstract::We determined the alterations in metabolic conversion of cysteine into glutathione and taurine in liver of rats treated with ethanol acutely. Ethanol treatment reduced cysteine as well as glutathione levels in liver for 24 h. However, cysteine dioxygenase was up-regulated rapidly, and hypotaurine/taurine levels were s...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-1969-2
更新日期:2015-06-01 00:00:00
abstract::The sulfur-containing amino acid, taurine (Tau), regulates glucose and lipid homeostasis under normal, pre- and diabetic conditions. Here, we aimed to verify whether Tau supplementation exerts its beneficial effects against obesity, hyperglycemia and alterations in islet functions, in leptin-deficient obese (ob/ob), o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-2553-3
更新日期:2018-06-01 00:00:00
abstract::In plasma and serum, the presence of high-abundance proteins can overwhelm the signals of low-abundance proteins, which then become undetectable either by two-dimensional gels or chromatographic techniques. Therefore, depletion of abundant proteins is a prerequisite to detect low-abundance components. Furthermore, the...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0556-6
更新日期:2008-04-01 00:00:00
abstract::The synthesis of enantiomerically pure 2-aminocyclobutanecarboxylic acids has been refined to improve both the efficiency and the simplicity. These improvements provide a shorter and easier access to the racemic cis-cyclobutane β-amino acid core. Derivatization of this material with a chiral non-racemic oxazolidin-2-o...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-0918-y
更新日期:2011-08-01 00:00:00
abstract::The glutamate/cystine antiporter system x(c)(-) transports cystine into cells in exchange for glutamate at a ratio of 1:1. It is composed of a specific light chain, xCT, and a heavy chain, 4F2, linked by a disulfide bridge. Intracellularly, cystine is reduced into cysteine, the rate-limiting precursor of glutathione (...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-011-0862-x
更新日期:2012-01-01 00:00:00
abstract::Schiff bases represent a class of molecules widely studied for their importance in organic and coordination chemistry. Despite the large amount of studies on the chemical and biological properties of the Schiff bases, the different experimental conditions prevent a useful comparison to search for a correlation structu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-019-02816-0
更新日期:2020-03-01 00:00:00
abstract::Animals at the neonatal stage have to eat more to support better growth and health. However, it is difficult to understand the mechanism of feeding during an early stage of life in the brain of the rodent model. Chickens are precocial and they can look for their food by themselves right after hatching. Neonatal chicks...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-019-02762-x
更新日期:2019-08-01 00:00:00