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Application of a serum-free medium in the growth and differentiation of human peripheral blood lymphocytes.

Abstract：

:To eliminate variability due to nonspecific stimulation or inhibition by different lots of fetal bovine serum, the activation of human peripheral blood mononuclear cells in a modification of Iscove's medium (medium C-IMDM) was compared with the routinely used Roswell Park Memorial Institute (RPMI)-1640 medium containing 15% fetal bovine serum. Stimulation of peripheral blood mononuclear cells was enhanced and occurred at lower mitogen concentrations in C-IMDM compared with cells grown in RPMI-1640 supplemented with fetal bovine serum. Maximum incorporation of [3H]thymidine following stimulation by concanavalin A, phytohemagglutinin, and pokeweed mitogen was more than twice the peak values obtained in RPMI-1640 supplemented with fetal bovine serum. Concentrations of concanavalin A and phytohemagglutinin required for maximum stimulation were 0.5 and 15 micrograms/ml, and 0.3 and 1.0 micrograms/ml . 5 X 10(5) cells in C-IMDM and RPMI-1640, respectively. Cells grown in C-IMDM responded to lower concentrations of pokeweed mitogen and optimal growth in the serum-free medium required 0.4 micrograms/ml . 5 X 10(5) cells. The stimulation of immunoglobulin-producing cells in C-IMDM was enhanced and occurred at lower concentrations of pokeweed mitogen. Less variability of growth (i.e., incorporation of [3H]thymidine) and immunoglobulin synthesis occurred in peripheral blood mononuclear cells cultured in different preparations of C-IMDM than that reported for cells cultured in RPMI-1640 supplemented with different lots or batches of fetal bovine serum. These data suggest that C-IMDM may be an alternative to media supplemented with fetal bovine serum.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Lipson SM

doi

10.1016/0732-8893(86)90099-4

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

203-14

issue

3

eissn

0732-8893

issn

1879-0070

pii

0732-8893(86)90099-4

journal_volume

4

pub_type

杂志文章

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