当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Evaluation of the fully automated AIX1000 rapid plasma reagin system compared to a manual plasma reagin testing method for the diagnosis of syphilis.

Evaluation of the fully automated AIX1000 rapid plasma reagin system compared to a manual plasma reagin testing method for the diagnosis of syphilis.

Abstract：

:The analytical performance of the FDA-cleared AIX1000 automated RPR testing platform was evaluated in comparison to manual RPR card testing. Eight hundred thirty-three patient serum samples were analyzed, 87 samples were positive by the AIX1000, 108 were positive by the manual test method; overall agreement between methods was 96.5% (κ = 0.83). Cases were further classified by clinical and laboratory-based confirmation of disease, to which reactivity rates were compared, yielding sensitivities of 96.4% and 100%, and specificities of 99.2% and 96.8% for the automated and manual RPR methods, respectively. The difference in specificity between methods was statistically significant (P < 0.001). Twenty-five of 29 samples with discordant results were reactive by manual testing (titers of 1:1 or 1:2); 21 of 25 patients with negative AIX100 results were identified to have histories of remote, treated syphilis. Overall, the AIX1000 platform demonstrated excellent agreement with the manual RPR method; discrepancies occurred with specimens at the threshold of reactivity.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Pyden A,Kang AD,Amato S,Dutta S,Berg G,Riedel S

doi

10.1016/j.diagmicrobio.2020.115081

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

115081

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(20)30458-2

journal_volume

97

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • rpoB gene high-resolution melt curve analysis: a rapid approach for diagnosis and screening of drug resistance in tuberculous meningitis.

    abstract：:Timely and rapid diagnosis of multidrug resistance in tuberculous meningitis (TBM) is a challenge both for a microbiologist and neurologist. The present study was conducted to evaluate role of real-time polymerase chain reaction (PCR) using rpoB, IS6110, and MPB64 as targets in diagnosis of TBM in 110 patients and sub...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.06.010

    authors： Sharma K,Modi M,Kaur H,Sharma A,Ray P,Varma S

    更新日期：2015-10-01 00:00:00

  • Susceptibility testing of Clostridium difficile against metronidazole and vancomycin by disk diffusion and Etest.

    abstract：:A prospective study on the susceptibility of Clostridium difficile to metronidazole and vancomycin using the Etest and disk diffusion test was performed over a 6-month period. One hundred strains were tested; one strain was highly resistant to metronidazole (MIC = 64 micrograms/mL). The zone size of inhibition by the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(98)00139-4

    authors： Wong SS,Woo PC,Luk WK,Yuen KY

    更新日期：1999-05-01 00:00:00

  • Evaluation of a commercially available cytomegalovirus-specific IgM test for the diagnosis of cytomegalovirus infection in the symptomatic newborn.

    abstract：:A commercially available (Electro-Nucleonics, Inc.) indirect fluorescent antibody test was evaluated for the detection of cytomegalovirus-specific IgM antibody in serum of newborn infants. The serum IgM fraction from 13 symptomatic newborns with cytomegalovirus infection and 30 control infants was tested. Seven of 13 ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90007-1

    authors： Thomson RB Jr,Benedict ML,Congeni BL

    更新日期：1987-07-01 00:00:00

  • Clinical and operational factors associated with treatment duration for cellulitis in outpatient parenteral antimicrobial therapy (OPAT).

    abstract：:This study aims to identify factors associated with duration of intravenous (IV) and follow-on oral antibiotic therapy for cellulitis in patients treated through outpatient parenteral antimicrobial therapy (OPAT). A retrospective review of episodes of cellulitis treated over a year (January 2018-January 2019) at a lar...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115305

    authors： Palit J,Cole J,Durojaiye OC

    更新日期：2021-01-06 00:00:00

  • A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis.

    abstract：:A cocktail of affinity-purified antibodies against diagnostically useful Mycobacterium tuberculosis H37Ra excretory-secretory protein antigens ES-31, ES-43, and EST-6 was explored for detection of circulating free and immune-complexed (IC) antigen in sera of patients with confirmed tuberculosis (TB) by sandwich enzyme...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.10.021

    authors： Harinath BC,Kumar S,Roy SS,Hirudkar S,Upadhye V,Shende N

    更新日期：2006-05-01 00:00:00

  • Linezolid-resistant Enterococcus faecium isolated from a patient without prior exposure to an oxazolidinone: report from the SENTRY Antimicrobial Surveillance Program.

    abstract：:A patient case report describes an Enterococcus faecium strain isolated from a blood culture that was resistant to linezolid (MIC, 8 microg/mL; G2576U mutation of 23S rRNA). Co-resistances were identified for vancomycin, ampicillin, macrolides, fluoroquinolones, chloramphenicol, rifampin, gentamicin (high-level), nitr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00333-9

    authors： Jones RN,Della-Latta P,Lee LV,Biedenbach DJ

    更新日期：2002-02-01 00:00:00

  • Rapid differentiation between clinically relevant mycobacteria in microscopy positive clinical specimens and mycobacterial isolates by line probe assay.

    abstract：:The Inno LiPA Mycobacteria assay, based on PCR amplification of the 16-23S rRNA spacer region of Mycobacterium species, has been designed for identification of mycobacteria grown in culture media and discrimination between Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, M. gordonae, M. xe...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00406-6

    authors： Johansen IS,Lundgren BH,Thyssen JP,Thomsen VØ

    更新日期：2002-08-01 00:00:00

  • Molecular identification and susceptibility profile in vitro of the emerging pathogen Candida kefyr.

    abstract：:Candida kefyr has been increasingly associated with bloodstream infections. This study reports on the identification and antifungal susceptibility pattern of 33 Candida isolates, identified biochemically as C. kefyr. One strain was finally identified as Candida sphaerica (Kluyveromyces lactis) by ITS sequencing. Both ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.06.007

    authors： Gomez-Lopez A,Pan D,Cuesta I,Alastruey-Izquierdo A,Rodriguez-Tudela JL,Cuenca-Estrella M

    更新日期：2010-01-01 00:00:00

  • Assessment of a new test to detect Legionella urinary antigen for the diagnosis of Legionnaires' Disease.

    abstract：:Given that the rate of mortality by Legionella pneumonia increases in incorrectly treated patients, rapid diagnosis and early antibiotic treatment are needed. We have assessed the performance of a new enzyme immunoassay (EIA) test (Bartels Inc. Trinity Biotech Company, Wicklow, Ireland) to detect Legionella pneumophil...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00308-x

    authors： Domínguez J,Galí N,Blanco S,Pedroso P,Prat C,Matas L,Ausina V

    更新日期：2001-12-01 00:00:00

  • In vitro evaluation of cefepime and other broad-spectrum beta-lactams in 22 medical centers in Japan: a phase II trial comparing two annual organism samples. The Japan Antimicrobial Resistance Study Group.

    abstract：:An antimicrobial resistance surveillance study in Japan is presented representing the second year (Phase II) results from 22 medical centers. Each participant laboratory tested (Etest, AB BIODISK, Solna, Sweden) 100 organisms, 10 strains each from 10 species groups including Escherichia coli, Klebsiella spp., Enteroba...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0732-8893(99)00120-0

    authors： Lewis MT,Yamaguchi K,Biedenbach DJ,Jones RN

    更新日期：1999-12-01 00:00:00

  • Genetic mutations in linezolid-resistant Mycobacterium avium complex and Mycobacterium abscessus clinical isolates.

    abstract：:There are no studies evaluating the mechanisms driving linezolid resistance in nontuberculous mycobacteria. The novel mutations G2599A and A2137T in the 23S rRNA gene and mutations A439G and G443A in the rplD gene associated with linezolid resistance were identified in linezolid-resistant M. avium complex isolates. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.10.022

    authors： Kim SY,Jhun BW,Moon SM,Jeon K,Kwon OJ,Huh HJ,Lee NY,Shin SJ,Daley CL,Koh WJ

    更新日期：2019-05-01 00:00:00

  • Activity of ceftazidime-avibactam against Escherichia coli isolates from U.S. veterans (2011) in relation to co-resistance and sequence type 131 (ST131) H30 and H30Rx status.

    abstract：:Escherichia coli ST131, with its multidrug-resistance-associated H30R1 and H30Rx clonal subsets within the H30R subclone, causes most antimicrobial-resistant E. coli infections. The activity of ceftazidime-avibactam (CZA) against ST131 strains is undefined. We determined CZA MICs for 595 E. coli clinical isolates from...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115034

    authors： Johnston BD,Thuras PD,Johnson JR

    更新日期：2020-07-01 00:00:00

  • Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay.

    abstract：:Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbanc...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00362-0

    authors： Quevedo Diaz MA,Melnicáková J,Kazár J,Hoch J,Senfleben S,Lukácová M

    更新日期：2002-04-01 00:00:00

  • Improved urine screening using a combination of leukocyte esterase and the Lumac system.

    abstract：:Three rapid urine screening tests, leukocyte esterase, nitrite, and the Lumac System for detection of bacterial ATP, were evaluated alone and in combination to determine their utility in screening urine specimens from male patients for bacteruria. The combination of leukocyte esterase and Lumac testing resulted in sig...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(85)90036-7

    authors： Pfaller MA,Scharnweber G,Stewart B,Koontz FP

    更新日期：1985-05-01 00:00:00

  • Comparative in vitro activities of ampicillin, BMY 28142, and imipenem against Mycobacterium avium complex.

    abstract：:The in vitro activity of ampicillin, BMY 28142, and imipenem was evaluated against 21 clinical isolates of Mycobacterium avium complex by both a broth and an agar dilution method. The MIC90 by broth dilution for ampicillin, BMY 28142, and imipenem was 16 micrograms/ml, 8 micrograms/ml, and greater than 32 micrograms/m...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90100-3

    authors： Cynamon MH,Palmer GS,Sorg TB

    更新日期：1987-02-01 00:00:00

  • Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry.

    abstract：:The application of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth has potential to identify bloodstream infection earlier and facilitate timely management. We prospectively tested a novel, rapid, and inexpensive in-house spin-lysis protocol...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.06.016

    authors： Gray TJ,Thomas L,Olma T,Iredell JR,Chen SC

    更新日期：2013-10-01 00:00:00

  • Discrepancies between in vitro activity of and in vivo response to antimicrobial agents.

    abstract：:The correlation between in vitro antimicrobial activity and the in vivo response to antimicrobial therapy is affected by multiple host factors, the site and nature of the infection, and the pharmacokinetics of the antimicrobial and its penetration into areas of infection. In certain instances, discrepancies are also c...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(83)90029-9

    authors： Washington JA 2nd

    更新日期：1983-03-01 00:00:00

  • Endocarditis caused by Finegoldia magna (formerly Peptostreptococcus magnus): diagnosis depends on the blood culture system used.

    abstract：:We present a patient with prosthetic valve endocarditis caused by Finegoldia magna (formerly Peptostreptococcus magnus). Blood cultures in the BacT/ALERT and BACTEC 9240 system were negative. We therefore tested different blood culture systems: F. magna grew in the SEPTI-CHEK BHI-S and in the ISOLATOR, but not in the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00091-9

    authors： Bassetti S,Laifer G,Goy G,Fluckiger U,Frei R

    更新日期：2003-09-01 00:00:00

  • Oral ofloxacin for infections caused by bacteria resistant to oral antimicrobial agents.

    abstract：:We conducted an open trial of oral ofloxacin, 400 mg q 12 hr, in the treatment of infections caused by Pseudomonas aeruginosa and other bacteria resistant to traditional oral antibiotics. There were 53 evaluable subjects, 30 having infection of the skin/skin structure, 13 of the respiratory tract, six of bone, and fou...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/0732-8893(92)90085-8

    authors： Eron LJ,Gentry LO

    更新日期：1992-07-01 00:00:00

  • Impact of CLIA 88 on the clinical microbiology laboratory.

    abstract：:The Clinical Laboratory Improvement Amendments (CLIA 88) to the Clinical Laboratory Improvement Act of 1967 continues to undergo transformation since its implementation more than 2 years ago. The law and its subsequent regulatory modifications were intended to promote high quality in and accurate results from laborato...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(95)00152-2

    authors： Chapin K,Baron EJ

    更新日期：1995-09-01 00:00:00

  • Evidence of nosocomial spread of Candida albicans causing bloodstream infection in a neonatal intensive care unit.

    abstract：:Candida albicans is an increasingly important bloodstream pathogen. We investigated a cluster of bloodstream infections in the neonatal intensive care unit (NICU) to determine whether nosocomial transmission occurred. Subjects included any patient in the NICU who developed clinically significant bloodstream infection ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(95)00048-f

    authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

    更新日期：1995-04-01 00:00:00

  • Comparison of interferon gamma-inducible protein-10 and interferon gamma-based QuantiFERON TB Gold assays with tuberculin skin test in HIV-infected subjects.

    abstract：:We aimed to compare the positivity of the QuantiFERON TB gold in-tube (QFT-IT antigens) specific interferon gamma (IFN-γ/QFT-IT) and IFN-γ-inducible protein-10 (IP-10/QFT-IT) assays with tuberculin skin test (TST) among human immunodeficiency virus (HIV)-infected individuals in a TB endemic setting. A total of 180 HIV...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.07.012

    authors： Kabeer BS,Sikhamani R,Raja A

    更新日期：2011-11-01 00:00:00

  • A highly sensitive 1-tube nested real-time RT-PCR assay using LNA-modified primers for detection of respiratory syncytial virus.

    abstract：:Respiratory syncytial virus (RSV) causes serious respiratory tract infection worldwide. The relatively low RSV load makes it difficult to detect in frail, elderly, and severely immune-compromised patients. In the present study, we developed a locked nucleic acid--based 1-tube nested real-time RT-PCR (OTNRT-PCR) assay ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.09.001

    authors： Zhao L,Wang J,Li GX,Qiu FZ,Chen C,Zhao MC,Wang L,Duan SX,Feng ZS,Ma XJ

    更新日期：2019-02-01 00:00:00

  • Detection of herpes simplex virus in clinical specimens by DNA hybridization.

    abstract：:An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 X 10(4) plaque-forming units of cell-free HSV and as few as four virus-...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(83)90041-x

    authors： Redfield DC,Richman DD,Albanil S,Oxman MN,Wahl GM

    更新日期：1983-06-01 00:00:00

  • Comparison of Gram and Kopeloff stains in the diagnosis of bacterial vaginosis in pregnancy.

    abstract：:Bacterial vaginosis (BV) is commonly diagnosed by using the Nugent score, a semiquantitative scoring system to evaluate bacterial morphotypes on Gram stain of vaginal secretions. Some authors have suggested using the Kopeloff modification of the Gram stain. Asymptomatic BV in pregnancy has been associated with adverse...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.09.017

    authors： Libman MD,Kramer M,Platt R,Montreal Prematurity Study Group.

    更新日期：2006-03-01 00:00:00

  • Characterization of extended-spectrum beta-lactamases, antimicrobial resistance genes, and plasmid content in Escherichia coli isolates from different sources in Rio de Janeiro, Brazil.

    abstract：:We analyse the diversity of extended-spectrum beta-lactamases (ESBLs), antibiotic resistance genes, and plasmid content among Escherichia coli from a community and a hospital setting in Rio de Janeiro, Brazil. ESBL producers (CTX-M-2, CTX-M-9, CTX-M-59) belonged to different phylogroups/sequence types, and bla(CTX-M) ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.05.019

    authors： Queiroz ML,Antunes P,Mourão J,Merquior VL,Machado E,Peixe LV

    更新日期：2012-09-01 00:00:00

  • In vitro activity of tedizolid against staphylococci isolated from prosthetic joint infections.

    abstract：:We determined the MIC and minimum biofilm bactericidal concentration (MBBC) of tedizolid and vancomycin against 97 isolates of Staphylococcus aureus and 74 isolates of Staphylococcus epidermidis associated with prosthetic joint infection. All isolates were vancomycin susceptible in the planktonic state; all staphyloco...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.01.008

    authors： Schmidt-Malan SM,Greenwood Quaintance KE,Karau MJ,Patel R

    更新日期：2016-05-01 00:00:00

  • Eggerthella hongkongensis sp. nov. and eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia.

    abstract：:Eggerthella, one of the human gut flora, was rarely reported to cause bacteremia in the literature. We describe the application of 16S ribosomal RNA gene sequencing in defining the epidemiology and clinical significance of Eggerthella bacteremia during a 4-year period. Among 55 clinically significant blood culture iso...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.04.012

    authors： Lau SK,Woo PC,Woo GK,Fung AM,Wong MK,Chan KM,Tam DM,Yuen KY

    更新日期：2004-08-01 00:00:00

  • Evaluation of the modified Visuwell Strep-A enzyme immunoassay for detection of group-A Streptococcus from throat swabs.

    abstract：:The modified Visuwell Strep-A enzyme immunoassay (EIA) was compared with culture for detection of group-A Streptococcus from throat swabs. Throat swabs in modified Stuarts medium obtained after culture at two institutions were tested in Visuwell. Cumulative results were n = 417, sensitivity 87.8%, specificity 89.9% pr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(91)90017-a

    authors： Drulak M,Bartholomew W,LaScolea L,Amsterdam D,Gunnersen N,Yong J,Fijalkowski C,Winston S

    更新日期：1991-07-01 00:00:00

  • Susceptibility of 100 blood isolates of Pseudomonas aeruginosa to 19 antipseudomonal antibiotics: old and new.

    abstract：:The in vitro susceptibility of 19 antipseudomonal antibiotics, old and new, were tested against 100 blood isolates of Pseudomonas aeruginosa. The isolates were taken from consecutive bacteremic patients hospitalized in nine Pittsburgh hospitals from 1983 to 1984. Twelve percent of isolates tested were tobramycin-resis...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90027-7

    authors： Korvick J,Yu VL,Hilf M

    更新日期：1987-06-01 00:00:00