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Rapid Chagas diagnosis in clinical settings using a multiparametric assay.

Abstract：

:The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombinant protein and a T. cruzi whole extract) placed in parallel in separate solid phases. This automated diagnostic tool allows the simultaneous analysis of a large number of sera, by determining the presence of antibodies against both antigens by a single run test, with high sensitivity and specificity. The simultaneous analysis of the reactivity against the 2 antigens in a biparametric modality reduces the percentage of false-negative sera and allows a more accurate diagnosis. Using this multiparametric approach, we propose an effective algorithm for the first step of Chagas diagnosis by performing a single test, with time and cost savings.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Pierimarchi P,Cerni L,Alarcón de Noya B,Nicotera G,Díaz-Bello Z,Angheben A,Scacciatelli D,Zonfrillo M,Recinelli G,Serafino A

doi

10.1016/j.diagmicrobio.2012.12.005

subject

Has Abstract

pub_date

2013-04-01 00:00:00

pages

381-9

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(12)00556-1

journal_volume

75

pub_type

杂志文章

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