Abstract:
:We developed and evaluated a method to genotypically identify enterotoxigenic Escherichia coli (ETEC) and to characterize these organisms with respect to 18 of 21 known colonization factors (CFs). The method, which is based on polynucleotide DNA-DNA colony hybridization, includes a pooled toxin probe assay to identify ETEC, and individual probe assays to detect the enterotoxins STp, STh, and LT, and the CFs CFA/I, CS1-CS8, CS12-CS15, CS17-CS19, CS21, and CS22. We evaluated the pooled toxin probe assay during a cohort study of childhood diarrhea, and the individual probe assays against 33 reference strains and 92 clinical ETEC isolates. There was close to a complete agreement between the pooled toxin probe assay and the individual toxin probe assays, and between the individual CF probe assays and the corresponding phenotypic assays.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Steinsland H,Valentiner-Branth P,Grewal HM,Gaastra W,Mølbak K K,Sommerfelt Hdoi
10.1016/s0732-8893(02)00504-7subject
Has Abstractpub_date
2003-02-01 00:00:00pages
97-105issue
2eissn
0732-8893issn
1879-0070pii
S0732889302005047journal_volume
45pub_type
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