Clinical evaluation of the improved streptex method for grouping streptococci.


:The improved Streptex method for serogrouping streptococci incorporates a new extraction enzyme and a simplified procedure requiring no centrifugation. A total of 114 clinical isolates of beta-hemolytic streptococci were serogrouped from primary plates, isolation plates, and Todd-Hewitt broth cultures using this system. Results were compared to those of the heat extraction Lancefield precipitin method. An additional 33 stock culture isolates of related streptococcal species and 5 strains of Listeria were serogrouped to assess the specificity of the test. Agreement between the two methods was 82.5% with primary plates and 96.5% with both isolation plates and broth cultures. Four isolates from three different serogroups were nongroupable by the Lancefield method, but did agglutinate in specific Streptex antisera; therefore, the enzyme extraction procedure appeared more sensitive than the heat extraction method. Streptex accurately grouped five isolates of gamma-hemolytic group B streptococci, but failed to detect antigen in 33% of the group D streptococcal extracts tested. In addition, cross-reactions were observed with strains of alpha-hemolytic streptococci. Streptex produced fewer ambiguous results and required fewer repeat tests. When used with isolation plates or broth cultures, Streptex is both sensitive and specific for the grouping of beta-hemolytic streptococci of groups A, B, C, F, and G.


Bixler-Forell E,Martin WJ,Moody MD




Has Abstract


1984-04-01 00:00:00














  • Impact of AmpC overexpression on outcomes of patients with Pseudomonas aeruginosa bacteremia.

    abstract::AmpC overexpression (AmpC++) is a significant mechanism of beta-lactam resistance in Pseudomonas aeruginosa, but its impact on clinical outcomes is not well established. To examine the influence of AmpC++ on clinical outcomes of patients with P. aeruginosa bacteremia, we screened all bloodstream P. aeruginosa isolates...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Tam VH,Chang KT,Schilling AN,LaRocco MT,Genty LO,Garey KW

    更新日期:2009-03-01 00:00:00

  • Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction.

    abstract::We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophore...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Miyawaki H,Fujita J,Takigawa K,Negayama K,Yamagishi Y,Yamaji Y,Ouchi K,Nakazawa T,Kawanishi K,Takahara J

    更新日期:1995-11-01 00:00:00

  • Rapid identification of Branhamella catarrhalis. A comparison of five rapid methods.

    abstract::Five methods for the rapid identification and differentiation of Branhamella catarrhalis from other Neisseria species in 86 respiratory specimens were compared. These tests included the 4-methylumbelliferyl butyrate (MUB), API quadFerm, B.CAT.Confirm, Gonochek II, and the Tributyrin disc. All five tests reliably and a...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Louie M,Ongsansoy EG,Forward KR

    更新日期:1990-05-01 00:00:00

  • Detection of beta-lactamase-negative ampicillin resistance in Haemophilus influenzae in Belgium.

    abstract::Haemophilus influenzae, a frequent colonizer of the respiratory tract, is the causative agent of several clinically important infections. In cases that require therapeutic intervention, laboratory susceptibility testing can detect beta-lactam antibiotic resistance and guide the best treatment course. In the absence of...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Schotte L,Wautier M,Martiny D,Piérard D,Depypere M

    更新日期:2019-03-01 00:00:00

  • Occurrence of the vanA and vanC2/C3 genes in Enterococcus species isolated from poultry sources in Malaysia.

    abstract::Enterococcus species isolated from poultry sources were characterized for their resistance to antibiotics, plasmid content, presence of van genes and their diversity by randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). The results showed that all isolates were multi-resistance to the antibiotics...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Radu S,Toosa H,Rahim RA,Reezal A,Ahmad M,Hamid AN,Rusul G,Nishibuchi M

    更新日期:2001-03-01 00:00:00

  • Corynebacterium jeikeium (group JK diphtheroids) endocarditis. A report of five cases.

    abstract::Corynebacterium jeikeium is the name recently given to a group of coryneform rods, formerly known as the JK group, and associated with a wide range of serious infections. We describe five patients with C. jeikeium endocarditis observed in two Belgian hospitals over a period of 5 years. In three cases, the infection oc...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Vanbosterhaut B,Surmont I,Vandeven J,Wauters G,Vandepitte J

    更新日期:1989-05-01 00:00:00

  • Rapid Chagas diagnosis in clinical settings using a multiparametric assay.

    abstract::The development of an immunoassay for detecting circulating antibodies against Trypanosoma cruzi with a good performance appears to be crucial in clinical settings for the management of Chagas disease. Here we propose a new automated ELISA test performed on serum samples using 2 different T. cruzi antigens (a recombin...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Pierimarchi P,Cerni L,Alarcón de Noya B,Nicotera G,Díaz-Bello Z,Angheben A,Scacciatelli D,Zonfrillo M,Recinelli G,Serafino A

    更新日期:2013-04-01 00:00:00

  • Partial comparison of the NxTAG Respiratory Pathogen Panel Assay with the Luminex xTAG Respiratory Panel Fast Assay V2 and singleplex real-time polymerase chain reaction for detection of respiratory pathogens.

    abstract::In this study, 185 nasopharyngeal swabs were tested to compare the sensitivity and specificity of the Luminex NxTAG (NxTAG) Respiratory Pathogen Panel (RPP) Assay with those of the Luminex Respiratory Virus Panel (RVP) Fast Assay v2 and singleplex real-time polymerase chain reaction (PCR). The NxTAG Assay identified a...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Esposito S,Scala A,Bianchini S,Presicce ML,Mori A,Sciarrabba CS,Fior G,Principi N

    更新日期:2016-09-01 00:00:00

  • In vitro susceptibility of Mycobacterium avium complex to antibacterial agents.

    abstract::In vitro agar dilution susceptibility studies were performed utilizing 20 isolates (24 against rifamycin) of Mycobacterium avium complex against several antimicrobial agents not routinely tested in the mycobacteriology laboratory. Thirteen strains were susceptible to gentamicin at 4 micrograms/ml, 20 to amikacin at 8 ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Davis CE Jr,Carpenter JL,Trevino S,Koch J,Ognibene AJ

    更新日期:1987-11-01 00:00:00

  • Optimized serodiagnosis of Mycoplasma pneumoniae infections.

    abstract::Serologic methods are well established for the diagnosis of Mycoplasma pneumoniae infections in humans, but they are less sensitive than polymerase chain reaction (PCR). To improve their sensitivity, a new panel of antigens was tested. Compared with PCR results, up to 92% of PCR-positive patients were confirmed by our...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Dumke R,Strubel A,Cyncynatus C,Nuyttens H,Herrmann R,Lück C,Jacobs E

    更新日期:2012-06-01 00:00:00

  • Disseminated "Haemophilus quentini" infection in a patient with multiple myeloma - a case report and review of the literature.

    abstract::We describe a case report of a 56-year-old male with undiagnosed multiple myeloma who had severe sepsis associated with pneumonia, meningitis, polyarthritis, and osteomyelitis related to invasive "Haemophilus quentini" infection. The genus was misidentified as H. influenzae by the common bacterial identification syste...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审


    authors: Cohen R,Finn T,Babushkin F,Karalnik S,Paikin S,Adler A,Geffen Y,Rokney A,Ron M

    更新日期:2019-07-01 00:00:00

  • Use of surrogate antimicrobial agents to predict susceptibility to ertapenem.

    abstract::Broth or agar dilution susceptibility test results for Enterobacteriaceae (11,775 strains), anaerobes (2888 strains), staphylococci (2206 strains), Haemophilus spp. (840 strains), group A streptococci (280 strains), group B streptococci (269 strains), Streptococcus pneumoniae (709 strains), and 160 other streptococci ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Friedland IR,Isaacs R,Mixson LA,Motyl M,Woods GL

    更新日期:2002-05-01 00:00:00

  • Candidemia due to Candida tropicalis: clinical, epidemiologic, and microbiologic characteristics of 188 episodes occurring in tertiary care hospitals.

    abstract::Candida tropicalis is the 2nd most frequent agent of candidemia in Brazil (20-24%). We attempted to characterize the epidemiology, microbiology, and outcome of candidemia due to C. tropicalis by comparing patients with candidemia due to C. tropicalis with those with candidemia due to Candida albicans. Among the 924 ep...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Nucci M,Colombo AL

    更新日期:2007-05-01 00:00:00

  • Superantigen profiling of Staphylococcus aureus infective endocarditis isolates.

    abstract::The frequency of superantigen production among Staphylococcus aureus isolates associated with endocarditis is not well defined. We tested 154 S. aureus isolates from definite infective endocarditis cases for the presence of staphylococcal enterotoxins A-E, H, and TSST-1 by PCR, enzyme-linked immunosorbent assay, and u...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Chung JW,Karau MJ,Greenwood-Quaintance KE,Ballard AD,Tilahun A,Khaleghi SR,David CS,Patel R,Rajagopalan G

    更新日期:2014-06-01 00:00:00

  • Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers.

    abstract::Rapid and accurate diagnostic tests for Clostridium difficile infections (CDI) are crucial for management of patients with suspected CDI and for infection control. Enzyme immunoassays for detection of the toxins are routinely used but lack adequate sensitivity. We generated slow off-rate modified aptamers (SOMAmer™ re...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Ochsner UA,Katilius E,Janjic N

    更新日期:2013-07-01 00:00:00

  • Determination of disk diffusion and MIC quality control guidelines for GSK2251052: a novel boron-containing antibacterial.

    abstract::GSK2251052 is a boron-containing antimicrobial agent in clinical development for the treatment of serious Gram-negative bacterial infections. These GSK2251052 quality control (QC) studies were performed to establish ranges for control strains (broth microdilution [BMD] MIC and disk diffusion zones) as follows: Pseudom...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Ross JE,Scangarella-Oman N,Jones RN

    更新日期:2013-04-01 00:00:00

  • Host and microbiologic factors associated with mortality in Taiwanese children with invasive pneumococcal diseases, 2001 to 2006.

    abstract::We concurrently evaluated host- and organism-related factors in determining the outcomes of 160 invasive pneumococcal diseases episodes in 158 Taiwanese children during 2001 and 2006. Most (138/160, 86.2%) episodes occurred at age less than 60 months, and an underlying condition was present in 35 (22.2%) cases. Common...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Chen CJ,Lin CL,Chen YC,Wang CW,Chiu CH,Lin TY,Huang YC

    更新日期:2009-02-01 00:00:00

  • Two cases of acute hepatitis after a serial knife stabbing.

    abstract::Three persons became victims of a serial knife stabbing in a fight. Three months later, one of the victims presented with acute hepatitis. On the fifth day of his admission to the hospital, another victim of this serial stabbing presented to our clinic with acute hepatitis B infection. The patients told that the first...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Bodur H,Onguru P,Akıncı E,Kanyılmaz D,Yetkin A,Ozdemir B,But A,Eren S

    更新日期:2012-11-01 00:00:00

  • Assessment of a new test to detect Legionella urinary antigen for the diagnosis of Legionnaires' Disease.

    abstract::Given that the rate of mortality by Legionella pneumonia increases in incorrectly treated patients, rapid diagnosis and early antibiotic treatment are needed. We have assessed the performance of a new enzyme immunoassay (EIA) test (Bartels Inc. Trinity Biotech Company, Wicklow, Ireland) to detect Legionella pneumophil...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Domínguez J,Galí N,Blanco S,Pedroso P,Prat C,Matas L,Ausina V

    更新日期:2001-12-01 00:00:00

  • Fusarium verticillioides fungemia in a liver transplantation patient: successful treatment with voriconazole.

    abstract::Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Cocchi S,Codeluppi M,Venturelli C,Bedini A,Grottola A,Gennari W,Cavrini F,Di Benedetto F,De Ruvo N,Rumpianesi F,Gerunda GE,Guaraldi G

    更新日期:2011-12-01 00:00:00

  • IS6110 dot blot hybridization for the identification of Mycobacterium tuberculosis complex.

    abstract::To explore a simple, rapid, and inexpensive way to identify Mycobacterium tuberculosis complex culture, dot blot hybridization using IS6110 as the marker was performed against 2788 known clinical isolates of mycobacteria including M. tuberculosis (n = 721), M. kansasii (177), M. marinum (10), M. avium complex (700), M...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Kam KM,Yip CW,Chan MY,Mok CY,Wong WS

    更新日期:1999-01-01 00:00:00

  • Insufficient diagnostic accuracy of imported serological kits for Helicobacter pylori infection in Japanese population.

    abstract::Although there are many reports of the high diagnostic accuracy of commercially available serologic kits for Helicobacter pylori infection in Western countries, they rarely has been investigated in oriental population. Accordingly we examined their usefulness in 492 Japanese patients with dyspeptic symptoms. Diagnosti...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章


    authors: Miwa H,Kikuchi S,Ohtaka K,Kobayashi O,Ogihara A,Hojo M,Nagahara A,Sato N

    更新日期:2000-02-01 00:00:00

  • Comparative activity of ciprofloxacin, ofloxacin, levofloxacin, and erythromycin against Legionella species by broth microdilution and intracellular susceptibility testing in HL-60 cells.

    abstract::Animal lung macrophages or human peripheral blood mononuclear cells have been used for testing intracellular activity of anti-Legionella antibiotics; such studies are labor intensive such that comparative antibiotic studies for the many Legionella species are few. We evaluated a human monocyte cell line (HL-60) as an ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Stout JE,Arnold B,Yu VL

    更新日期:1998-01-01 00:00:00

  • Comparison of susceptibility results of anaerobic organisms determined by agar dilution method and Sceptor Anaerobe MIC/ID Micro Broth Dilution Panels.

    abstract::A commercial broth microdilution system (Sceptor Anaerobe MIC/ID, BBL) for susceptibility testing of anaerobic bacteria was compared to a reference agar dilution system. Of the 172 organisms tested, only 7% failed to grow sufficiently for testing in the Sceptor system. In 1,590 antibiotic/organism combinations, 86.7% ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Hussain Z,Lannigan R,Schieven BC,Stoakes L,Groves D

    更新日期:1987-10-01 00:00:00

  • Erysipelothrix bacteremia without endocarditis: rare event or under-reported occurrence?

    abstract::A patient presented with inflamed hands and Erysipelothrix rhusiopathiae bacteremia. Because a high incidence of endocarditis has been reported with this organism, a transesophageal echocardiogram was obtained, which was normal. Treatment with oral moxifloxacin resolved all manifestations of illness. The association b...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Drekonja DM

    更新日期:2013-11-01 00:00:00

  • Effects of echinocandins on cytokine/chemokine production by human monocytes activated by infection with Candida glabrata or by lipopolysaccharide.

    abstract::Serious Candida glabrata infections, which can be difficult to treat, are often treated with echinocandins. We compared in vitro the effects of high and low concentrations of 3 echinocandins (micafungin [MCF], caspofungin [CAS], and anidulafungin [ANF]), voriconazole (VRC), and amphotericin B (AmB), singly and VRC in ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Baltch AL,Lawrence DA,Ritz WJ,Andersen NJ,Bopp LH,Michelsen PB,Carlyn CJ,Smith RP

    更新日期:2012-03-01 00:00:00

  • Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America.

    abstract::Chikungunya was introduced into the Americas in 2015 causing a pandemic across the continent. Testing during the acute phase of infection relies on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary strains....

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Edwards T,Del Carmen Castillo Signor L,Williams C,Larcher C,Espinel M,Theaker J,Donis E,Cuevas LE,Adams ER

    更新日期:2017-09-01 00:00:00

  • Epidemiology and outcome of multiple-species candidemia at a tertiary care center between 2004 and 2007.

    abstract::The incidence of multiple-species candidemia (MSC) among cases of candidemia ranges between 2.8% and 8.0%. We sought to study the epidemiology and outcome of MSC at a tertiary care center. A retrospective analysis of MSC episodes occurring between 2004 and 2007 was performed. MSC was defined as > or =2 different Candi...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Nace HL,Horn D,Neofytos D

    更新日期:2009-07-01 00:00:00

  • Comparison of CMY-2 plasmids isolated from human, animal, and environmental Escherichia coli and Salmonella spp. from Canada.

    abstract::A total of 244 CMY-2 plasmids from 5 separate studies involving Escherichia coli and Salmonella human clinical cases as well as E. coli from feedlots and water sources were examined. Genetically similar CMY-2 plasmids isolated from either E. coli or Salmonella from human, animal, and environmental sources are widely d...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Mataseje LF,Baudry PJ,Zhanel GG,Morck DW,Read RR,Louie M,Mulvey MR

    更新日期:2010-08-01 00:00:00

  • Rapid differentiation between clinically relevant mycobacteria in microscopy positive clinical specimens and mycobacterial isolates by line probe assay.

    abstract::The Inno LiPA Mycobacteria assay, based on PCR amplification of the 16-23S rRNA spacer region of Mycobacterium species, has been designed for identification of mycobacteria grown in culture media and discrimination between Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, M. gordonae, M. xe...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章


    authors: Johansen IS,Lundgren BH,Thyssen JP,Thomsen VØ

    更新日期:2002-08-01 00:00:00