Abstract：
:At our community teaching hospital between August 1994 and August 1995, Candida glabrata accounted for 14% of all Candida isolates and for 31% of urinary Candida isolates. The culture site was urine for 68% of C. glabrata isolates compared to 30% of all Candida isolates (p < 0.001, chi 2). To study the association between C. glabrata and isolation from the urine, we analyzed all available C. glabrata urinary isolates over a 3-month period (23 isolates from 20 patients) using electrophoretic karyotyping, random amplified polymorphic DNA analysis, and fluconazole susceptibility testing. Random amplified polymorphic DNA generated eight types, although electrophoretic karyotyping generated 17 types. Combining the two methods resulted in 19 types indicating that urinary C. glabrata strains at our hospital are genetically diverse and the association between C. glabrata and urinary tract isolation does not appear to be due to horizontal transmission of a single or small number of strains. In vitro susceptibility tests showed that C. glabrata isolates from patients receiving fluconazole had significantly higher minimum inhibitory concentrations to fluconazole than those not receiving fluconazole (p < 0.05). Despite a limited number of patients and isolates, our data suggest that selection of less susceptible organisms by the presence of antifungal agents may be an important contributor to increased urinary isolation of C. glabrata from patients in our hospital.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Schwab U,Chernomas F,Larcom L,Weems Jdoi
10.1016/s0732-8893(97)00076-xsubject
Has Abstractpub_date
1997-09-01 00:00:00pages
11-7issue
1eissn
0732-8893issn
1879-0070pii
S073288939700076Xjournal_volume
29pub_type
杂志文章abstract：:To compare the identification accuracies of VITEK 2 (bioMérieux), MicroScan (Siemens Healthcare), and Phoenix (Becton Dickinson), microbial identification was performed on 160 clinical isolates and 50 reference strains on each of these 3 systems, using the appropriate identification kit provided by each system. Of the...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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更新日期：2011-08-01 00:00:00
abstract：:In recent years, Clostridium spp. have rapidly reemerged as human and animal pathogens. The detection and identification of pathogenic Clostridium spp. is therefore critical for clinical diagnosis and antimicrobial therapy. Traditional diagnostic techniques for clostridia are laborious, are time consuming, and may adv...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.09.014
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abstract：:Enteroviruses were isolated from 14 of 31 frozen serum specimens obtained from hospitalized patients with enterovirus infection, which had been previously documented by recovery of virus from stool, throat, or cerebrospinal fluid (CSF). Virus was detected in 71% of the positive sera within 3 days of specimen inoculati...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(84)90069-5
更新日期：1984-09-01 00:00:00
abstract：:Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2014.02.009
更新日期：2014-06-01 00:00:00
abstract：:Phoma species are primarily phytopathogens which have been reported to sporadically cause human disease. We report a patient with phaeohyphomycotic cysts caused by Phoma species, which were initially mistaken for ganglions. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2011.04.009
更新日期：2011-08-01 00:00:00
abstract：:We report a 1-step assay to screen antiviral substances combining the simultaneous application of cells, virus sample, and drug to 96-well plates. The results were obtained within 26 h when vaccinia virus plaques were counted or virus-induced cytopathic effect was measured. This fast cost-effective procedure may be us...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.03.015
更新日期：2009-07-01 00:00:00
abstract：:A prospective multicenter study was conducted to assess the epidemiology of antimicrobial resistance among uropathogens causing uncomplicated cystitis. Adult women with clinical diagnosis of uncomplicated cystitis were enrolled from 54 participating centers distributed all over Hong Kong during 2006 to 2008. A positiv...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diagmicrobio.2009.03.027
更新日期：2010-01-01 00:00:00
abstract：:Four immunocompromised patients, immigrants from Ethiopia, presented with diverse clinical manifestations of meningitis associated with Strongyloides stercoralis dissemination as determined by identification of intestinal larvae. The cerebrospinal fluid of 3 patients was tested by a validated (for stool) real-time PCR...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2015.11.015
更新日期：2016-03-01 00:00:00
abstract：:Development of the ARCHITECT Toxo IgM assay has been done to assist the clinician in acute Toxoplasma gondii infection detection, especially in pregnant women. Its use, in conjunction with ARCHITECT Toxo IgG and Toxo Avidity assays, will provide an array of assays particularly useful in the monitoring of pregnant fema...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.03.016
更新日期：2009-07-01 00:00:00
abstract：:ST252 Enterobacter cloacae, producing GES-5 carbapenemase, was isolated in a Czech hospital. blaGES-5 was part of a novel class 1 integron, In1406, which also included a new allele of the aadA15 gene cassette. In1406 was located on a ColE2-like plasmid, pEcl-35771cz (6953bp). ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2017.10.011
更新日期：2018-02-01 00:00:00
abstract：:The presence of staphylococcal enterotoxin (SE) genes (seg, seh, sei, and sej) and the correlation of their prevalence with the genotypes were studied in 100 clinical isolates of Staphylococcus aureus. Polymerase chain reaction (PCR) of SE genes indicated that 39% of the isolates were enterotoxigenic. Thirty-seven per...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.04.002
更新日期：2006-10-01 00:00:00
abstract：:We tested three fluoroquinolones (ciprofloxacin, levofloxacin, and trovafloxacin), each combined with each of four beta-lactams (cefoperazone, ceftriaxone, imipenem, and meropenem) for synergy against clinical isolates of nosocomial strains of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepa...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(98)00126-6
更新日期：1999-02-01 00:00:00
abstract：:Bacteria harboring CTX-M extended-spectrum beta-lactamases (ESBLs) have been identified worldwide, with most reports coming from regions outside North America. We have identified CTX-M enzymes in 31% of ESBL-positive Escherichia coli isolates from our hospital and more than half (53%) of the isolates from associated l...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.11.012
更新日期：2010-04-01 00:00:00
abstract：:Early diagnosis of leptospirosis in humans is critical with regard to initiation of appropriate treatment; however, the gold standard serological test cannot detect antibodies until nearly a week after symptom onset. PCR has been shown to be sensitive and specific in the early phase of leptospirosis. Previously, we de...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2015.03.024
更新日期：2015-07-01 00:00:00
abstract：:We examined the in vitro activities of voriconazole, posaconazole, and fluconazole against 3,932 isolates of Candida spp. and 237 isolates of Cryptococcus neoformans obtained from over 100 medical centers worldwide during 2001 and 2002. The MICs of the antifungal drugs were determined by broth microdilution tests perf...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2003.09.008
更新日期：2004-03-01 00:00:00
abstract：:Ceftaroline fosamil was approved in 2010 by the United States Food and Drug Administration (USA-FDA) for the treatment of patients with acute bacterial skin and skin structure infections (ABSSSIs) and community-acquired bacterial pneumonia (CABP). After approval, several studies and case reports have described the pos...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.diagmicrobio.2014.11.016
更新日期：2015-03-01 00:00:00
abstract：:The ability of 57 Spanish microbiology laboratories in detecting and reporting beta-lactam resistance phenotypes in Escherichia coli and Klebsiella pneumoniae was evaluated. Laboratories received 6 well-characterized isolates expressing the most widespread extended-spectrum beta-lactamases (ESBLs) in Spain (4 CTX-M ty...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,多中心研究
doi：10.1016/j.diagmicrobio.2008.06.010
更新日期：2008-11-01 00:00:00
abstract：:The incidence of multiple-species candidemia (MSC) among cases of candidemia ranges between 2.8% and 8.0%. We sought to study the epidemiology and outcome of MSC at a tertiary care center. A retrospective analysis of MSC episodes occurring between 2004 and 2007 was performed. MSC was defined as > or =2 different Candi...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.03.010
更新日期：2009-07-01 00:00:00
abstract：:Cationic steroid antimicrobials (CSA-ceragenin) are a new class of antimicrobial agent. In vitro activities of CSA-8, CSA-13, CSA-44, CSA-131, and CSA-138 and amphotericin B (AMP-B) were assessed against 50 nonrepeat Candida spp. isolates MICs, MFCs and combination studies were determined. Antibiofilm activities of CS...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2018.03.014
更新日期：2018-08-01 00:00:00
abstract：:This study examined the serotype distribution and antimicrobial resistance of pneumococcal isolates from invasive infections in children between 2006 and 2010, when the 7-valent pneumococcal conjugate vaccine (PCV7) was offered as an optional vaccine in Korea. Among 140 isolates collected from 8 centers, the common se...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2013.12.016
更新日期：2014-04-01 00:00:00
abstract：:Diagnosis of Chlamydia psittaci and Chlamydia pneumoniae infections has traditionally relied on serological assays. We developed a multiplex real-time PCR assay for detection of C. psittaci, C. pneumoniae and an internal control. Results of this assay demonstrated 100% concordance compared to results of previously tes...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2017.11.014
更新日期：2018-03-01 00:00:00
abstract：:Microsatellite typing was used to analyze 41 Aspergillus fumigatus isolates from 9 patients with proven invasive aspergillosis hospitalized in 2 different centers. No strains were shared between patients. For 8 of 9 patients, a single genotype was found for the isolates recovered from all anatomic sites involved. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2008.04.006
更新日期：2008-09-01 00:00:00
abstract：:A rich store of detailed information about antimicrobial resistance is at each medical center in paper files inaccessible to analysis or in electronic files too diverse to support a common analytical software. WHONET puts that information on a personal computer at each center in a file code and format that is the same...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：
更新日期：1996-08-01 00:00:00
abstract：:The amikacin plus gentamicin-containing SuperAminoglycoside medium was developed for screening multiple-aminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2018.01.026
更新日期：2018-06-01 00:00:00
abstract：:The objective of this research was to develop group- and type-specific probes for the detection of enteroviruses. Coxsackie virus B4 RNA was cloned, and a series of subgenomic clones were generated. Six of these clones, containing sequences from the 3' end or the 5' end of the genome, were tested for their ability to ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(88)90014-4
更新日期：1988-11-01 00:00:00
abstract：:Resistance to linezolid among Enterococcus faecium and Enterococcus faecalis isolates has been reported in patients who receive a prolonged course of the drug. We report two cases of linezolid-resistant Enterococcus faecalis that occurred in patients who previously received linezolid for infections with vancomycin-res...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(03)00153-6
更新日期：2003-11-01 00:00:00
abstract：:Low antibody titers (3 to 6 IU/mL) detected by IMx and AxSYM Toxo IgG assays in serum samples from 264 pregnant women were confirmed by the dye test and a high-sensitivity agglutination test in, respectively, 98.5% and 95.6% of cases, attesting a premunition of these patients. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(98)00060-1
更新日期：1998-09-01 00:00:00
abstract：:In this study we evaluated the performance of the SHIGA TOXIN QUIK CHEK (Techlab®, Blacksburg, VA) and the ImmunoCard STAT! Enterohaemorrhagic E. coli (EHEC) (Meridian BioScience, Cincinnati, OH, USA) assays as methods for qualitatively detecting the presence of Shiga toxin in human fecal specimens. A multiplex PCR fo...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2016.03.011
更新日期：2017-02-01 00:00:00
abstract：:Antibiotic-loaded acrylic bone cements (ALABC) spacers are routinely used in the treatment of prosthetic joint infections. The objectives of our study were to evaluate different ALABC for elution kinetics, thermal stability, and mechanical properties. A 10 or 20% mixture (w/w) beads of medium viscosity bone cement (De...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2013.09.014
更新日期：2014-01-01 00:00:00
abstract：:Although there are many reports of the high diagnostic accuracy of commercially available serologic kits for Helicobacter pylori infection in Western countries, they rarely has been investigated in oriental population. Accordingly we examined their usefulness in 492 Japanese patients with dyspeptic symptoms. Diagnosti...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 临床试验,杂志文章
doi：10.1016/s0732-8893(99)00143-1
更新日期：2000-02-01 00:00:00
