当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Development of a microarray for identification of pathogenic Clostridium spp.

Development of a microarray for identification of pathogenic Clostridium spp.

Abstract：

:In recent years, Clostridium spp. have rapidly reemerged as human and animal pathogens. The detection and identification of pathogenic Clostridium spp. is therefore critical for clinical diagnosis and antimicrobial therapy. Traditional diagnostic techniques for clostridia are laborious, are time consuming, and may adversely affect the therapeutic outcome. In this study, we developed an oligonucleotide diagnostic microarray for pathogenic Clostridium spp. The microarray specificity was tested against 65 Clostridium isolates. The applicability of this microarray in a clinical setting was assessed with the use of mock stool samples. The microarray was successful in discriminating at least 4 species with the limit of detection as low as 10(4) CFU/mL. In addition, the pattern of virulence and antibiotic resistance genes of tested strains were determined through the microarrays. This approach demonstrates the high-throughput detection and identification of Clostridium spp. and provides advantages over traditional methods. Microarray-based techniques are promising applications for clinical diagnosis and epidemiologic investigations.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Janvilisri T,Scaria J,Gleed R,Fubini S,Bonkosky MM,Gröhn YT,Chang YF

doi

10.1016/j.diagmicrobio.2009.09.014

subject

Has Abstract

pub_date

2010-02-01 00:00:00

pages

140-7

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(09)00400-3

journal_volume

66

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Bacteroides fragilis in biopsies of patients with major abscesses and diabetic foot infections: direct molecular versus culture-based detection.

    abstract：:Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.12.017

    authors： Stappers MH,Hagen F,Reimnitz P,Mouton JW,Meis JF,Gyssens IC

    更新日期：2016-06-01 00:00:00

  • Prevalence and risk factors for carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae among patients prior to bowel surgery.

    abstract：:Prophylactic antibiotics are an important measure in preventing perioperative infection, Failure to cover multidrug-resistant pathogens may place carriers at increased risk of infection. We conducted a prospective, cross-sectional study in patients prior to bowel surgery to measure the carriage prevalence of extended-...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.04.002

    authors： Pfeffer I,Zemel M,Kariv Y,Mishali H,Adler A,Braun T,Klein A,Matalon MK,Klausner J,Carmeli Y,Schwaber MJ

    更新日期：2016-07-01 00:00:00

  • Comparative in vitro activity of cefoxitin, cefotaxime alone, and in combination with desacetylcefotaxime against the Bacteroides species.

    abstract：:The agar dilution method was used to determine the inhibitory activity of cefotaxime (CTX) alone, desacetylcefotaxime (dCTX) alone, CTX plus dCTX, and cefoxitin against 74 clinical isolates of the Bacteroides species recovered from diabetic patients with foot ulcers. The study concluded that the addition of dCTX to CT...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(89)90043-6

    authors： Canawati HN

    更新日期：1989-01-01 00:00:00

  • Trends in antimicrobial susceptibilities among bacterial pathogens isolated from patients hospitalized in European medical centers: 6-year report of the MYSTIC Surveillance Study (1997-2002).

    abstract：:The Meropenem Yearly Susceptibility Test Information Collection (MYSTIC) Program provides antimicrobial susceptibility data. The activity of meropenem and 7 broad-spectrum antimicrobials have been examined against 12645 bacterial isolates from 14 European centers between 1997 and 2002. Cumulative susceptibility rates ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.11.013

    authors： Turner PJ

    更新日期：2005-04-01 00:00:00

  • Etest for susceptibility testing of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare.

    abstract：:Methods currently used for susceptibility testing of Mycobacterium, including agar dilution and the Bactec radiometric method, are based on the proportion technique using a single critical concentration of antibiotic. Preliminary studies were conducted with Mycobacterium species by using the Etest method, a gradient m...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(94)90064-7

    authors： Wanger A,Mills K

    更新日期：1994-07-01 00:00:00

  • The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction.

    abstract：:A 10-min fecal preparation results in greater specimen turbidity than a 45-min protocol, but reverse transcriptase polymerase chain reaction (RT-PCR) norovirus test sensitivity is essentially the same. Feces processed so that particle size does not exceed approximately 560 nm do not display greater norovirus RT-PCR in...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.08.006

    authors： Witlox KJ,Karapanagiotidis T,Bruggink LD,Marshall JA

    更新日期：2010-02-01 00:00:00

  • Diagnostic value of F-18 FDG PET/CT in patients with spondylodiscitis: Is dual time point imaging time worthy?

    abstract：PURPOSE:In this retrospective study, we aimed to investigate the value of FDG-PET/CT in the diagnosis of spondylodiscitis (SD), the significance of dual time point imaging (DTPI) for SD diagnosis and the worth of SUVmax data for distinguishing tuberculous vs. non-tuberculous SD. MATERIALS AND METHODS:32 patients with ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.03.021

    authors： Gunes BY,Onsel C,Sonmezoglu K,Ozaras R,Halac M,Tabak F,Sager S,Mete B,Sayman H,Kanmaz B

    更新日期：2016-07-01 00:00:00

  • High doses of daptomycin (10 mg/kg/d) plus rifampin for the treatment of staphylococcal prosthetic joint infection managed with implant retention: a comparative study.

    abstract：:We aimed to analyze the efficacy and safety of high doses of daptomycin (10 mg/kg/d) plus rifampin (D10 + R) for prosthetic joint infection (PJI). This was an observational retrospective multicenter study (2010-2012) including all patients with acute PJI by fluoroquinolone-resistant staphylococci managed with implant ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.diagmicrobio.2014.05.022

    authors： Lora-Tamayo J,Parra-Ruiz J,Rodríguez-Pardo D,Barberán J,Ribera A,Tornero E,Pigrau C,Mensa J,Ariza J,Soriano A

    更新日期：2014-09-01 00:00:00

  • Comparison of real-time polymerase chain reaction and conventional biochemical methods for identification of Mycobacterium chelonae-Mycobacterium abscessus group to the species level.

    abstract：:The Mycobacterium chelonae-Mycobacterium abscessus group (MCAG) is the most common cause of infections because of rapidly growing mycobacteria. Rapid identification of MCAG to the species level is essential for choosing empiric antibiotic treatment and for public health measures. In this study, we compared the perform...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.03.011

    authors： Guarin N,Budvytiene I,Ghafghaichi L,Banaei N

    更新日期：2010-08-01 00:00:00

  • Reproducibility of positive results for rare pathogens on the FilmArray GI Panel.

    abstract：:Though the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for selected targets were ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.03.013

    authors： Hitchcock MM,Hogan CA,Budvytiene I,Banaei N

    更新日期：2019-09-01 00:00:00

  • Antimicrobial susceptibility of Streptococcus pyogenes in Central, Eastern, and Baltic European Countries, 2005 to 2006: the cefditoren surveillance program.

    abstract：:The in vitro activity of penicillin, ampicillin, cefditoren, cefotaxime, erythromycin, clarithromycin, and levofloxacin against 763 clinical isolates of Streptococcus pyogenes was determined. Clinically significant isolates collected from November 2005 to December 2006 in the Czech Republic, Slovakia, Hungary, Poland,...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.12.018

    authors： Gracia M,Díaz C,Coronel P,Gimeno M,García-Rodas R,Rodríguez-Cerrato V,del Prado G,Huelves L,Ruiz V,Naves PF,Ponte MC,Granizo JJ,Soriano F

    更新日期：2009-05-01 00:00:00

  • Diagnostic performance of a new automated heterophile antibody test in adults and children.

    abstract：:Epstein-Barr virus serologic profiles were obtained from 618 patients using an automated platform, including the new BioPlex heterophile assay. When compared with 2 IgM viral capsid antigen (VCA) assays and a predicate heterophile assay, the BioPlex heterophile assay was more likely to be associated with serologic pat...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.02.016

    authors： Klutts JS,Wu AH,Smith A,Yen-Lieberman B,Gronowski AM

    更新日期：2008-07-01 00:00:00

  • Comparative in vitro activities of ertapenem against aerobic and facultative bacterial pathogens from patients with complicated skin and skin structure infections.

    abstract：:This study compared the in vitro activities of ertapenem (Merck & Co., Inc.), ceftriaxone, amoxicillin-clavulanate, and piperacillin-tazobactam against 518 aerobic and facultative bacterial pathogens isolated from 340 patients with complicated skin and skin structure infections. Ciprofloxacin was also tested against G...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00387-5

    authors： Pelak BA,Bartizal K,Woods GL,Gesser RM,Motyl M

    更新日期：2002-06-01 00:00:00

  • Multicenter evaluation of the MB-Redox medium compared with radiometric BACTEC system, mycobacteria growth indicator tube (MGIT), and Löwenstein-Jensen medium for detection and recovery of acid-fast bacilli.

    abstract：:MB-Redox is a new manual culture system designed for the recovery of mycobacteria from clinical specimens. It consists of a liquid medium (modified Kirchner medium) containing a redox indicator, a colorless tetrazolium salt, which is reduced to colored formazan by actively growing mycobacteria. Acid fast bacilli (AFB)...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/s0732-8893(99)00029-2

    authors： Piersimoni C,Scarparo C,Cichero P,De Pezzo M,Covelli I,Gesu G,Nista D,Scagnelli M,Mandler F

    更新日期：1999-08-01 00:00:00

  • Prospective clinical validation of 3D printed nasopharyngeal swabs for diagnosis of COVID-19.

    abstract：:COVID-19 greatly disrupted the global supply chain of nasopharyngeal swabs, and thus new products have come to market with little data to support their use. In this prospective study, 2 new 3D printed nasopharyngeal swab designs were evaluated against the standard, flocked nasopharyngeal swab for the diagnosis of COVI...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115257

    authors： Oland G,Garner O,de St Maurice A

    更新日期：2020-10-22 00:00:00

  • Single gene target bacterial identification. groEL gene sequencing for discriminating clinical isolates of Burkholderia pseudomallei and Burkholderia thailandensis.

    abstract：:Proper identification of Burkholderia pseudomallei and Burkholderia thailandensis is crucial in guiding clinical management of patients with suspected melioidosis, as more than 99% of cases of melioidosis are caused by B. pseudomallei, whereas B. thailandensis is only responsible for causing less than 1% of the cases....

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00439-x

    authors： Woo PC,Woo GK,Lau SK,Wong SS,Yuen Ky

    更新日期：2002-10-01 00:00:00

  • Use of nucleic acid probes for the detection of sexually transmitted infectious agents.

    abstract：:Deoxyribonucleic acid sequences specific for a pathogen of interest can be isolated from a variety of microorganisms. Such DNA probes can be exploited to detect infectious agents directly in infected patient material despite the presence of large numbers of other organisms and host DNA. This technology is dependent up...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/s0732-8893(86)80048-7

    authors： Horn JE,Quinn T,Hammer M,Palmer L,Falkow S

    更新日期：1986-03-01 00:00:00

  • Value of the L-pyrrolidonyl-beta-naphthylamide hydrolysis test for identification of select gram-positive cocci.

    abstract：:A 4 hr test for hydrolysis of L-pyrrolidonyl-beta-naphthylamide (PYR) was evaluated for its use in identifying group A beta-hemolytic streptococci, group D enterococci, and Staphylococcus haemolyticus. All of the group A streptococci and the enterococci tested were positive in the PYR test, whereas all of the nongroup...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(86)90055-6

    authors： Oberhofer TR

    更新日期：1986-01-01 00:00:00

  • Comparison of microarray-predicted closest genomes to sequencing for poliovirus vaccine strain similarity and influenza A phylogeny.

    abstract：:We evaluate sequence data from the PathChip high-density hybridization array for epidemiological interpretation of detected pathogens. For influenza A, we derive similar relative outbreak clustering in phylogenetic trees from PathChip-derived compared to classical Sanger-derived sequences. For a positive polio detecti...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.11.003

    authors： Maurer-Stroh S,Lee CW,Patel C,Lucero M,Nohynek H,Sung WK,Murad C,Ma J,Hibberd ML,Wong CW,Simões EA

    更新日期：2016-03-01 00:00:00

  • Salmonella prosthetic valve endocarditis.

    abstract：:Four cases of Salmonella prosthetic valve endocarditis have been reported previously in the English medical literature (Fraser et al. 1967; Yamamoto et al., 1974; Shanson et al., 1977; Bassa et al., 1989). This report describes a fifth case in a 62-year-old woman who developed tricuspid valve endocarditis after an epi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/0732-8893(92)90124-c

    authors： Choo PW,Gantz NM,Anderson C,Maguire JH

    更新日期：1992-03-01 00:00:00

  • Cross-reactivity of non-Aspergillus fungal species in the Aspergillus galactomannan enzyme immunoassay.

    abstract：:The Aspergillus galactomannan enzyme-linked immunosorbant assay (EIA) has been demonstrated to facilitate rapid and sensitive detection of invasive aspergillosis. However, test specificity has not been fully evaluated in non-Aspergillus fungal species. Of 53 fungal isolates, cross-reactivity was observed with 5 non-As...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2007.04.022

    authors： Cummings JR,Jamison GR,Boudreaux JW,Howles MJ,Walsh TJ,Hayden RT

    更新日期：2007-09-01 00:00:00

  • Ceftaroline: clinical and microbiology experience with focus on methicillin-resistant Staphylococcus aureus after regulatory approval in the USA.

    abstract：:Ceftaroline fosamil was approved in 2010 by the United States Food and Drug Administration (USA-FDA) for the treatment of patients with acute bacterial skin and skin structure infections (ABSSSIs) and community-acquired bacterial pneumonia (CABP). After approval, several studies and case reports have described the pos...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/j.diagmicrobio.2014.11.016

    authors： Stryjewski ME,Jones RN,Corey GR

    更新日期：2015-03-01 00:00:00

  • Investigation of nosocomial respiratory infection due to Pseudomonas cepacia by arbitrarily primed polymerase chain reaction.

    abstract：:We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiologic investigation of Pseudomonas cepacia nosocomial isolates obtained from patients attending our hospital. This approach was compared with conventional phenotypic typing and pulsed-field gel electrophore...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(95)00176-x

    authors： Miyawaki H,Fujita J,Takigawa K,Negayama K,Yamagishi Y,Yamaji Y,Ouchi K,Nakazawa T,Kawanishi K,Takahara J

    更新日期：1995-11-01 00:00:00

  • Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis.

    abstract：:The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected case...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.06.021

    authors： Shinu P,Singh V,Nair A,Mehrishi P,Mehta S,Joshi E

    更新日期：2013-10-01 00:00:00

  • Molecular characterization of Staphylococcus aureus isolates carrying the Panton-Valentine leukocidin genes from Rio de Janeiro hospitals.

    abstract：:In a collection of 50 pvl-positive Staphylococcus aureus isolates from 10 Rio de Janeiro hospitals, 18 (36%) were from bloodstream infections, and 31 (62%) carried the SCCmec IV. Among 25 (50%) isolates of the USA1100/ST30/CC30 lineage present in 8 hospitals, 1 isolate was characterized as vancomycin-intermediate S. a...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.09.004

    authors： Chamon RC,Iorio NL,da Silva Ribeiro S,Cavalcante FS,Dos Santos KR

    更新日期：2015-12-01 00:00:00

  • Molecular identification and susceptibility profile in vitro of the emerging pathogen Candida kefyr.

    abstract：:Candida kefyr has been increasingly associated with bloodstream infections. This study reports on the identification and antifungal susceptibility pattern of 33 Candida isolates, identified biochemically as C. kefyr. One strain was finally identified as Candida sphaerica (Kluyveromyces lactis) by ITS sequencing. Both ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.06.007

    authors： Gomez-Lopez A,Pan D,Cuesta I,Alastruey-Izquierdo A,Rodriguez-Tudela JL,Cuenca-Estrella M

    更新日期：2010-01-01 00:00:00

  • Rapid differentiation between clinically relevant mycobacteria in microscopy positive clinical specimens and mycobacterial isolates by line probe assay.

    abstract：:The Inno LiPA Mycobacteria assay, based on PCR amplification of the 16-23S rRNA spacer region of Mycobacterium species, has been designed for identification of mycobacteria grown in culture media and discrimination between Mycobacterium tuberculosis complex, M. avium, M. intracellulare, M. kansasii, M. gordonae, M. xe...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00406-6

    authors： Johansen IS,Lundgren BH,Thyssen JP,Thomsen VØ

    更新日期：2002-08-01 00:00:00

  • The prevalence of staphylococcal resistance to penicillinase-resistant penicillins. A retrospective and prospective national surveillance trial of isolates from 40 medical centers.

    abstract：:In a retrospective survey of resistance to penicillinase-resistant penicillins (PRPs) in 152,076 clinical staphylococcal strains isolated in 40 United States Hospitals in 1985 and 1986, rates of resistance to oxacillin were found to be 11 and 13%, respectively, among Staphylococcus aureus isolates. The rates were appr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/0732-8893(89)90108-9

    authors： Jones RN,Barry AL,Gardiner RV,Packer RR

    更新日期：1989-09-01 00:00:00

  • Activities of ceftazidime, ceftaroline, and aztreonam alone and combined with avibactam against isogenic Escherichia coli strains expressing selected single β-lactamases.

    abstract：:Avibactam is a novel β-lactamase inhibitor that restores the activity of otherwise hydrolyzed β-lactams against Gram-negative bacteria expressing different classes of serine β-lactamases. In the last decade, β-lactam-avibactam combinations were tested against a variety of clinical isolates expressing multiple commonly...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.02.003

    authors： Papp-Wallace KM,Bajaksouzian S,Abdelhamed AM,Foster AN,Winkler ML,Gatta JA,Nichols WW,Testa R,Bonomo RA,Jacobs MR

    更新日期：2015-05-01 00:00:00

  • Sustained gastrointestinal colonization and systemic dissemination by Candida albicans, Candida tropicalis and Candida parapsilosis in adult mice.

    abstract：:The ability of nine clinical isolates of Candida species (three C. albicans, three C. tropicalis and three C. parapsilosis) to colonize and invade the gastrointestinal (GI) tract of adult male CD-1 (ICR) mice was determined. The effect of dietary tetracycline plus glucose supplementation on colonization was evaluated....

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(00)00165-6

    authors： Mellado E,Cuenca-Estrella M,Regadera J,González M,Díaz-Guerra TM,Rodríguez-Tudela JL

    更新日期：2000-09-01 00:00:00