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Detection of enterotoxin genes seg, seh, sei, and sej and of a novel aroA genotype in Jordanian clinical isolates of Staphylococcus aureus.

Abstract：

:The presence of staphylococcal enterotoxin (SE) genes (seg, seh, sei, and sej) and the correlation of their prevalence with the genotypes were studied in 100 clinical isolates of Staphylococcus aureus. Polymerase chain reaction (PCR) of SE genes indicated that 39% of the isolates were enterotoxigenic. Thirty-seven percent of the total isolates were seg positive, whereas 24% and 4% were sei and seh positive, respectively. All isolates containing sei were positive for seg, whereas sej gene was not detected. Genotyping by PCR-restriction fragment length polymorphism analysis of the aroA gene revealed that 39% of the isolates were type A and 11% were type B, and 50% displayed a novel (N) genotype. The presence of the enterotoxin genes was independent (P < 0.05) of the genotypes of the tested S. aureus isolates. This study has demonstrated that the seg was the most dominant enterotoxin gene and that the enterotoxigenic Jordanian S. aureus isolates belong to different genotypes, and N genotype was predominant.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

El-Huneidi W,Bdour S,Mahasneh A

doi

10.1016/j.diagmicrobio.2006.04.002

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

127-32

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(06)00156-8

journal_volume

56

pub_type

杂志文章

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