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Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium.

Abstract：

:Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal mucus and mucin on the growth and survival of two Staphylococcus aureus strains in liquid Amies transport medium at room temperature and 4 °C for 14 days. The study showed that the presence of nasal mucus in microbiological samples stimulated undesired S. aureus growth at room temperature in a dose-dependent manner. These findings underscore that microbiological samples from humans and animals should be stored at 4 °C until analysis to avoid undesired S. aureus growth.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Islam MZ,Larsen J,Skov R,Angen Ø

doi

10.1016/j.diagmicrobio.2018.07.009

subject

Has Abstract

pub_date

2019-01-01 00:00:00

pages

5-8

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30245-1

journal_volume

93

pub_type

杂志文章

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