Abstract：
:Three Copan viral transport media (VTM) were compared to the BBL Viral Culturette and Micro Test M4 systems for their ability to maintain the viability of clinical strains of enterovirus, herpes simplex virus, adenovirus and parainfluenza virus. VTM were inoculated, incubated up to 72 h at both 4 degrees C and 22 degrees C, and processed for cell culture. The concentration of infectious virus in VTM was determined by standard endpoint dilution assay. All VTM maintained viable enterovirus and adenovirus for 72 h but adenovirus from M4 and Copan CVM media were detected in cell culture at least one day sooner than from other media. Only M4 and Copan CVM media supported the recognition of low-titer herpes simplex virus in cell culture within 24 h. Parainfluenza virus was consistently detected in culture within six days when maintained in M4 and two of the Copan VTM media. Overall, the Copan CVM compared favorably to the M4 in maintaining concentrations of viable virus that could be recovered and identified rapidly in routine cell culture.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Dunn JJ,Billetdeaux E,Skodack-Jones L,Carroll KCdoi
10.1016/s0732-8893(02)00541-2subject
Has Abstractpub_date
2003-03-01 00:00:00pages
191-7issue
3eissn
0732-8893issn
1879-0070pii
S0732889302005412journal_volume
45pub_type
杂志文章abstract：:The emergence of the pandemic influenza virus A H1N1 has made fast and accurate diagnosis essential. However, few well-validated diagnostic techniques exist. The real-time RT-PCR developed by the Centers for Disease Control and Prevention (CDC) is the recommended technique. Our objective was to compare the CDC real-ti...
journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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pub_type： 杂志文章
doi：10.1016/s0732-8893(98)00052-2
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2017.12.011
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:Rifampin resistance in Mycobacterium tuberculosis could be determined within 2 h by using pyrosequencing-based approach. Pyrosequencing results of rifampin-resistant (n = 21) and rifampin-susceptible (n = 20) M. tuberculosis isolates were concordant with those based on drug susceptibility testing and conventional DNA ...
journal_title：Diagnostic microbiology and infectious disease
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doi：10.1016/j.diagmicrobio.2004.10.001
更新日期：2005-02-01 00:00:00
