Abstract：
:Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (30 species) and 434 clinical isolates of staphylococci were analyzed. Gene sequencing of 16S rRNA, gap or tuf genes was the reference method for species identification. All reference strains (100%) were correctly identified, while the identification rates of clinical isolates of S. aureus and CoNS were 98.9% and 98%, respectively. The sensitivity and specificity for mecA detection were 99% and 100%, respectively, in S. aureus isolates, and both values were 100% in isolates of CoNS. The assay takes 6 h from a purified culture isolate, and so far it has not been performed directly on patient samples.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Han HW,Chang HC,Chang TCdoi
10.1016/j.diagmicrobio.2016.06.003subject
Has Abstractpub_date
2016-09-01 00:00:00pages
23-9issue
1eissn
0732-8893issn
1879-0070pii
S0732-8893(16)30163-8journal_volume
86pub_type
杂志文章abstract：:Susceptibility testing using the EUCAST-AFST method against 39 clinical Fusarium strains consecutively collected from local and invasive infections during the last 10years assessed the in vitro activities of amphotericin B (AmB) and triazole antifungal agents. In addition, the susceptibility pattern of 12 reference st...
journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2019.03.010
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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abstract：:Borrelia burgdorferi sensu lato (s. l.), the agent of Lyme disease, is distributed widely worldwide. A large number of polymerase chain reaction (PCR) methods have been developed and used for detection of B. burgdorferi s. l. However, there is a lack of a reference standard because of the genetic diversity of the B. b...
journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,多中心研究
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2005.02.011
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
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更新日期：1987-11-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2014.07.010
更新日期：2014-11-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2010.07.006
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.07.017
更新日期：2007-03-01 00:00:00
abstract：:We conducted an open trial of oral ofloxacin, 400 mg q 12 hr, in the treatment of infections caused by Pseudomonas aeruginosa and other bacteria resistant to traditional oral antibiotics. There were 53 evaluable subjects, 30 having infection of the skin/skin structure, 13 of the respiratory tract, six of bone, and fou...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 临床试验,杂志文章
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更新日期：1992-07-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2013.01.024
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,随机对照试验
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abstract：:The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotyp...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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更新日期：2017-03-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.10.016
更新日期：2007-04-01 00:00:00
abstract：:This retrospective study aimed to evaluate the clinical, laboratory, and quantitative cerebrospinal fluid (CSF) cryptococcal cell counts for associations with in-hospital outcomes of HIV-infected patients with cryptococcal meningitis. Ninety-eight HIV-infected adult patients with CSF culture-proven cryptococcal mening...
journal_title：Diagnostic microbiology and infectious disease
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doi：10.1016/j.diagmicrobio.2012.01.014
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(01)00222-x
更新日期：2001-03-01 00:00:00
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(02)00392-9
更新日期：2002-07-01 00:00:00
abstract：:T2 Magnetic Resonance Candida Panel (T2MR) detects Candida directly in blood. Rapid turnaround time and high negative predictive value make it a useful diagnostic test to support antifungal discontinuation. This retrospective quasi-experiment compared empiric anidulafungin days of therapy (DOTs) in intensive care unit...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2019.04.016
更新日期：2019-10-01 00:00:00
abstract：:We report a case of mycotic keratitis caused by Bipolaris oryzae with predisposing trauma from a foreign body. The fungus was identified by sequencing the internal transcribed spacer region, translation elongation factor 1α (TEF1) gene, and partial glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene, and the species ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2015.11.020
更新日期：2016-05-01 00:00:00
abstract：:Intraabdominal sepsis is frequently seen following penetrating or blunt abdominal trauma as well as with perforated appendicitis or diverticulitis. The initial leakage of endogenous gastrointestinal microflora into the peritoneal cavity results in peritonitis and secondary septicemia, which often results in a localize...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/0732-8893(89)90136-3
更新日期：1989-07-01 00:00:00
abstract：:Antibodies to phenolic glycolipid (PGL)-I and major membrane protein (MMP)-II were evaluated for serodiagnosis of leprosy in Southwest China, and the role in predicting the occurrence of the disease in household contacts (HHCs) of leprosy was examined. Using PGL-I (natural disaccharide-octyl-bovine serum albumin) anti...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
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abstract：:Cupriavidus bacteremia is a rare infection and identification of the pathogen is difficult. We present four cases of bacteremia by Cupriavidus metallidurans that were initially identified to the genus level by both Bruker and Vitek matrix-assisted laser desorption ionization-time of flight mass spectrometry and later ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2014.09.015
更新日期：2015-01-01 00:00:00
