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Evaluation of a pan-serotype point-of-care rapid diagnostic assay for accurate detection of acute dengue infection.

Abstract：

:The catastrophic rise in dengue infections in India and globally has created a need for an accurate, validated low-cost rapid diagnostic test (RDT) for dengue. We prospectively evaluated the diagnostic performance of NS1/IgM RDT (dengue day 1) using 211 samples from a pediatric dengue cohort representing all 4 serotypes in southern India. The dengue-positive panel consisted of 179 dengue real-time polymerase chain reaction (RT-PCR) positive samples from symptomatic children. The dengue-negative panel consisted of 32 samples from dengue-negative febrile children and asymptomatic individuals that were negative for dengue RT-PCR/NS1 enzyme-linked immunosorbent assay/IgM/IgG. NS1/IgM RDT sensitivity was 89.4% and specificity was 93.8%. The NS1/IgM RDT showed high sensitivity throughout the acute phase of illness, in primary and secondary infections, in different severity groups, and detected all 4 dengue serotypes, including coinfections. This NS1/IgM RDT is a useful point-of-care assay for rapid and reliable diagnosis of acute dengue and an excellent surveillance tool in our battle against dengue.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Vivek R,Ahamed SF,Kotabagi S,Chandele A,Khanna I,Khanna N,Nayak K,Dias M,Kaja MK,Shet A

doi

10.1016/j.diagmicrobio.2016.09.020

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

229-234

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(16)30319-4

journal_volume

87

pub_type

杂志文章

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