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Development of a rapid detection method to detect tdh gene in Vibrio parahaemolyticus using 2-step ultrarapid real-time polymerase chain reaction.

Abstract：

:Thermostable direct hemolysin encoded by tdh gene has been considered an important virulence factor in pathogenic Vibrio parahaemolyticus. Two-step ultrarapid real-time polymerase chain reaction (URRT PCR) with a microchip was devised to detect V. parahaemolyticus carrying tdh gene. This novel method has a 6-μL reaction volume and extremely reduces running time since one cycle can be completed in 10 s or less. Consequently, 35 cycles of URRT PCR was successfully able to detect up to 100 fg (18 copies) of genomic DNA from pathogenic V. parahaemolyticus carrying tdh gene in 6 min. These results indicate that this method is at present the most rapid detection method for tdh gene and pathogenic V. parahaemolyticus.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Kang MH,Kim IW,Lee DW,Yoo MS,Han SH,Yoon BS

doi

10.1016/j.diagmicrobio.2010.08.020

subject

Has Abstract

pub_date

2011-01-01 00:00:00

pages

21-9

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(10)00349-4

journal_volume

69

pub_type

杂志文章

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