当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood cultures following isolation by lysis-centrifugation.

Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood cultures following isolation by lysis-centrifugation.

Abstract：

:We evaluated a direct from positive blood culture pelleting procedure that utilizes a lysis-centrifugation protocol for the identification of microorganisms by MALDI-TOF MS with subsequent antimicrobial susceptibility testing (AST) and rapid methicillin- and beta-lactam-resistance screening. The identification evaluation was performed on 125 cultures and resulted in the correct genus-level identification in 91.2% of cultures and a species-level concordance of 82.4% compared to routine subcultured growth. For the AST evaluation, susceptibility results from direct pelleting and subcultured growth for 187 cultures were compared; an average ±2-fold dilution agreement of 98.2% (1650/1681) and 98.6% (1375/1394) for Gram-negatives and -positives, respectively, was found. Major errors fell below 5% except for MRSA, which was falsely reported as oxacillin sensitive 17.2% (11/66) of the time. Lastly, the sensitivity and specificity of rapid MRSA screening were 94.7% (36/38) and 90.0% (9/10), respectively, while the ESBL screening results were 90.3% (65/72) and 100.0% (13/13) respectively.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Campigotto A,Goneau L,Matukas LM

doi

10.1016/j.diagmicrobio.2018.06.010

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

189-193

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30196-2

journal_volume

92

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Reproducibility of gemifloxacin and comparison fluoroquinolone MIC results using Sensititre commercial dry-form panels.

    abstract：:This study examined the ability of commercial dry-form broth microdilution panels (Sensititre, TREK Diagnostics, Cleveland, OH) to produce accurate minimum inhibitory concentration (MIC) results for gemifloxacin, a new fluoroquinolone. Reproducibility for gemifloxacin was 100.0% for on-scale values within +/-1 log2 di...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.11.005

    authors： Ross JE,Sader HS,Fritsche TR,Jones RN

    更新日期：2005-03-01 00:00:00

  • Clinical and operational factors associated with treatment duration for cellulitis in outpatient parenteral antimicrobial therapy (OPAT).

    abstract：:This study aims to identify factors associated with duration of intravenous (IV) and follow-on oral antibiotic therapy for cellulitis in patients treated through outpatient parenteral antimicrobial therapy (OPAT). A retrospective review of episodes of cellulitis treated over a year (January 2018-January 2019) at a lar...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115305

    authors： Palit J,Cole J,Durojaiye OC

    更新日期：2021-01-06 00:00:00

  • Antimicrobial activity of cefepime tested against Bush group I beta-lactamase-producing strains resistant to ceftazidime. A multilaboratory national and international clinical isolate study.

    abstract：:The potency of cefepime, a parenteral aminothiazolyl methoxyimino cephalosporin, was assessed against 256 ceftazidime-resistant Gram-negative bacilli from five medical centers in the United States. In addition, cefepime activity was compared with that of ciprofloxacin and imipenem against 506 ceftazidime-resistant Gra...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/0732-8893(94)90048-5

    authors： Jones RN,Marshall SA

    更新日期：1994-05-01 00:00:00

  • Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice.

    abstract：:Coxiella burnetii, a Gram-negative intracellular bacterium, can give rise to Q fever in humans and is transmitted mainly by inhalation of infected aerosols from animal reservoirs. Serology is commonly used to diagnose Q fever, but the early cellular immune response-i.e., C. burnetii-specific interferon γ (IFN-γ) produ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2014.12.008

    authors： Schoffelen T,Self JS,Fitzpatrick KA,Netea MG,van Deuren M,Joosten LA,Kersh GJ

    更新日期：2015-04-01 00:00:00

  • In vitro pharmacodynamic activity of gatifloxacin, gemifloxacin, moxifloxacin and levofloxacin against Streptococcus pneumoniae containing specific mutations in DNA gyrase and topoisomerase IV.

    abstract：:An in vitro pharmacodynatnic modeling apparatus (PDMA) generated specific bacterial kill profiles for single-dose regimens of gatifloxacin (GT), gemifloxacin (GM), moxifloxacin (MX) and levofloxacin (LV) against isolates of Streptococcus pneumoniae with specific QRDR profiles: SP-WT (no modifications); SP-C (changes i...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00152-4

    authors： Garrison MW,Schimmels JA,Madaras-Kelly KJ

    更新日期：2003-12-01 00:00:00

  • Pichia ohmeri fungemia.

    abstract：:A patient with a history of diabetes, coronary artery disease, stroke, previous renal transplantation, and multiple hospital admissions for recurrent pancreatitis was transferred to the hospital from a chronic care facility because of fever and severe epigastric discomfort. At the time of admission, she was receiving ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(97)00233-2

    authors： Bergman MM,Gagnon D,Doern GV

    更新日期：1998-03-01 00:00:00

  • Comparison of LED and conventional fluorescence microscopy for detection of acid-fast bacilli in an area with high tuberculosis incidence.

    abstract：:The objective of the study is to compare the performance of conventional fluorescence microscopy (CFM) and light-emitting diode (LED) fluorescence microscopy (FM) for detection of acid-fast bacilli (AFB) in clinical samples. We included AFB smears, stained using the auramine O method and blindly examined with both CFM...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.03.023

    authors： Marzouk M,Ferjani A,Dhaou M,Ali MH,Hannachi N,Boukadida J

    更新日期：2013-07-01 00:00:00

  • Trends in nalidixic acid resistance in nontyphoidal Salmonella isolated from 1999 to 2002: decreased susceptibility to 6 fluoroquinolones.

    abstract：:We determined the resistance to nalidixic acid and 6 fluoroquinolones of 771 nontyphoidal Salmonella strains isolated from humans between 1999 and 2002. A total of 22 different serotypes were identified among the Salmonella isolates, the most common being Salmonella Enteritidis (79%) and Salmonella Derby (8%). Resista...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.02.013

    authors： Rodriguez-Avial I,Rodriguez-Avial C,López O,Picazo JJ

    更新日期：2005-07-01 00:00:00

  • Serological and molecular detection of Mycoplasma pneumoniae in children with community-acquired lower respiratory tract infections.

    abstract：:This study was designed to evaluate the incidence of Mycoplasma pneumoniae infection in children with community-acquired lower respiratory tract infections (LRTIs). A total of 245 patients 6 months to 12 years of age were investigated for M. pneumoniae employing serological tests, polymerase chain reaction (PCR), nest...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.03.010

    authors： Kumar S,Garg IB,Sethi GR

    更新日期：2019-09-01 00:00:00

  • Antimicrobial activities of doripenem and other carbapenems against Pseudomonas aeruginosa, other nonfermentative bacilli, and Aeromonas spp.

    abstract：:The in vitro activity of doripenem against prevalent nonfermentative Gram-negative bacilli and Aeromonas spp. was evaluated. The collection comprised 14979 nonduplicate clinical isolates submitted during a global Doripenem Surveillance Program conducted from 2003 through 2007. Susceptibility tests were performed using...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.01.026

    authors： Castanheira M,Jones RN,Livermore DM

    更新日期：2009-04-01 00:00:00

  • Fusarium verticillioides fungemia in a liver transplantation patient: successful treatment with voriconazole.

    abstract：:Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.08.024

    authors： Cocchi S,Codeluppi M,Venturelli C,Bedini A,Grottola A,Gennari W,Cavrini F,Di Benedetto F,De Ruvo N,Rumpianesi F,Gerunda GE,Guaraldi G

    更新日期：2011-12-01 00:00:00

  • Evidence of nosocomial spread of Candida albicans causing bloodstream infection in a neonatal intensive care unit.

    abstract：:Candida albicans is an increasingly important bloodstream pathogen. We investigated a cluster of bloodstream infections in the neonatal intensive care unit (NICU) to determine whether nosocomial transmission occurred. Subjects included any patient in the NICU who developed clinically significant bloodstream infection ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(95)00048-f

    authors： Reagan DR,Pfaller MA,Hollis RJ,Wenzel RP

    更新日期：1995-04-01 00:00:00

  • CMY-2, CMY-8b, and DHA-1 plasmid-mediated AmpC β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae from a university hospital, Thailand.

    abstract：:Between February 2005 and January 2006 in Srinagarind Hospital, Thailand, 44 from 1730 isolates (2.5%) of Escherichia coli and 8 from 982 isolates (0.8%) of Klebsiella pneumoniae were found to produce plasmid-mediated AmpC β-lactamases (pAmpCs) as detected by a cefoxitin-Hodge test followed by a multiplex polymerase c...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.06.014

    authors： Singtohin S,Chanawong A,Lulitanond A,Sribenjalux P,Auncharoen A,Kaewkes W,Songsri J,Pienthaweechai K

    更新日期：2010-11-01 00:00:00

  • Clinical evaluation of a urine transport kit with lyophilized preservative for culture, urinalysis, and sediment microscopy.

    abstract：:Comparative results of urine culture, specific gravity, chemical urinalysis, and sediment microscopy were assessed when specimens submitted to the clinical laboratory were divided and processed either in a transport kit containing a lyophilized preservative or in a conventional sterile cup without preservative. Overal...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(85)80006-7

    authors： Weinstein MP

    更新日期：1985-11-01 00:00:00

  • Increased bacterial adherence and biomass in Pseudomonas aeruginosa bacteria exposed to clarithromycin.

    abstract：:Long-term low-dose macrolides alter response in patients with chronic sessile Pseudomonas aeruginosa colonization. We examined the effect of clarithromycin on 1) adherence of P. aeruginosa cells and 2) biofilm formation. A suspended-coupon continuous-flow biofilm reactor model was used. Adherent P. aeruginosa bacteria...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.09.007

    authors： Garey KW,Vo QP,Lewis RE,Saengcharoen W,LaRocco MT,Tam VH

    更新日期：2009-01-01 00:00:00

  • Comparative in vitro assessment of sparfloxacin activity and spectrum using results from over 14,000 pathogens isolated at 190 medical centers in the USA. SPAR Study Group.

    abstract：:Sparfloxacin, a new orally administered fluoroquinolone, was tested against 14,182 clinical strains isolated (generally blood stream and respiratory tract cultures) at nearly 200 hospitals in the United States (USA) and Canada. Sparfloxacin activity was compared with 13 other compounds by Etest (AB BIODISK, Solna, Swe...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(97)81807-x

    authors： Ballow CH,Jones RN,Johnson DM,Deinhart JA,Schentag JJ

    更新日期：1997-11-01 00:00:00

  • Cross-over assessment of serum bactericidal activity of moxifloxacin and levofloxacin versus penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae in healthy volunteers.

    abstract：:We compared the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against penicillin-susceptible and penicillin-resistant Streptococcus pneumoniae in 12 healthy volunteers. Each subject received 3 days of oral moxifloxacin 400 mg daily and levofloxacin 750 mg daily, respectively, with a 2- to 4-week w...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.diagmicrobio.2007.02.006

    authors： Hart D,Weinstein MP

    更新日期：2007-07-01 00:00:00

  • Sustained gastrointestinal colonization and systemic dissemination by Candida albicans, Candida tropicalis and Candida parapsilosis in adult mice.

    abstract：:The ability of nine clinical isolates of Candida species (three C. albicans, three C. tropicalis and three C. parapsilosis) to colonize and invade the gastrointestinal (GI) tract of adult male CD-1 (ICR) mice was determined. The effect of dietary tetracycline plus glucose supplementation on colonization was evaluated....

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(00)00165-6

    authors： Mellado E,Cuenca-Estrella M,Regadera J,González M,Díaz-Guerra TM,Rodríguez-Tudela JL

    更新日期：2000-09-01 00:00:00

  • Performance of a molecular viability assay for the diagnosis of Pneumocystis pneumonia in HIV-infected patients.

    abstract：:Pneumocystis pneumonia (PCP), caused by infection with Pneumocystis jirovecii, remains an important opportunistic infection in humans. A reverse transcriptase polymerase chain reaction assay has been shown to specifically detect viable P. jirovecii organisms. In the current study, we evaluated this assay on different ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.08.015

    authors： de Oliveira A,Unnasch TR,Crothers K,Eiser S,Zucchi P,Moir J,Beard CB,Lawrence GG,Huang L

    更新日期：2007-02-01 00:00:00

  • Anti-Mycobacterium avium complex activity of clarithromycin, rifampin, rifabutin, and ethambutol in combination with adenosine 5'-triphosphate.

    abstract：:We previously reported that adenosine 5'-triphosphate (ATP) inhibited the growth of various bacteria, including mycobacteria, Staphylococcus, and Pseudomonas, without damaging bacterial surface structures. Notably, ATP's antibacterial activity was found to be attributable to its iron-chelating ability. ATP exhibited c...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.04.012

    authors： Tatano Y,Yamabe S,Sano C,Tomioka H

    更新日期：2017-07-01 00:00:00

  • Development of a 1-step cell-based assay for cost-effective screening of antiviral drugs for vaccinia virus.

    abstract：:We report a 1-step assay to screen antiviral substances combining the simultaneous application of cells, virus sample, and drug to 96-well plates. The results were obtained within 26 h when vaccinia virus plaques were counted or virus-induced cytopathic effect was measured. This fast cost-effective procedure may be us...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.03.015

    authors： Jesus DM,Santos ES,Schnellrath LC,Damaso CR

    更新日期：2009-07-01 00:00:00

  • Major methodology-dependent discordant susceptibility results for Bacteroides fragilis group isolates but not other anaerobes.

    abstract：:Two standardized susceptibility test methods, a broth microdilution (BMD) and agar dilution (AD) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. Against the 339 strains of the Bacteroides fragilis group BMD mi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(94)90107-4

    authors： Aldridge KE,Schiro DD

    更新日期：1994-11-01 00:00:00

  • Natural antimicrobial susceptibility patterns and biochemical identification of Escherichia albertii and Hafnia alvei strains.

    abstract：:Bangladeshi diarrheagenic Hafnia alvei-like strains have been described recently as the new species Escherichia albertii (Int J Syst Evolut Microbiol. 2003;53:807-810). The natural susceptibility of 21 E. albertii and 76 H. alvei strains to 69 antimicrobial agents was examined, applying a microdilution procedure in Is...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.10.008

    authors： Stock I,Rahman M,Sherwood KJ,Wiedemann B

    更新日期：2005-03-01 00:00:00

  • Bacteriological studies with ampicillin/sulbactam on selected strains of gram-negative bacteria.

    abstract：:Antibacterial activity of sulbactam or ampicillin alone and in combination on ampicillin-resistant, beta-lactamase-producing Gram-negative bacteria (Citrobacter freundii and Escherichia coli) was studied. Inhibition of beta-lactamase activity by sulbactam was investigated using intact and disrupted cells. Minimal inhi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(88)90079-x

    authors： Bello H,del Solar E,González C,Garcia A,Urrea R,Zemelman R

    更新日期：1988-10-01 00:00:00

  • Four year prospective evaluation of nosocomial bacteremia: epidemiology, microbiology, and patient outcome.

    abstract：:A prospective study of all patients with clinically significant nosocomial bacteremia at one institution from 1994 to 1997 was performed to: (1) describe the epidemiology and microbiology of nosocomial bacteremias; (2) determine the crude mortality associated with such infections; and (3) identify independent predicto...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(00)00192-9

    authors： Lark RL,Chenoweth C,Saint S,Zemencuk JK,Lipsky BA,Plorde JJ

    更新日期：2000-11-01 00:00:00

  • Impact of mutations in hVISA isolates on decreased susceptibility to vancomycin, through population analyses profile - area under curve (PAP-AUC).

    abstract：:We analyzed sequences of graSR, vraSR, walKR and rpoB genes in hVISA from Brazil. Five isolates showed mutations in at least one gene. rpoB H481N and graS T224I were the most frequent mutations, followed by graR D148Q and walK A468T. Our study reinforces the heterogeneity of genetic patterns among hVISA. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.06.006

    authors： Silveira ACO,Caierão J,Silva CI,Anzai EK,McCulloch JA,d'Azevedo PA,Sincero TCM

    更新日期：2019-11-01 00:00:00

  • Three different patterns of positive Clostridium difficile laboratory tests. A comparison of clinical behavior.

    abstract：:Clostridium difficile (CD) diagnosis is very varied and under discussion. Different research groups disagree on the clinical significance of patients with negative direct toxin and positive polymerase chain reaction (PCR) or even more with direct toxin and glutamate dehydrogenase (GDH) both negatives, but CD detected ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115050

    authors： Olmedo M,Alcalá L,Valerio M,Marín M,Onori R,Reigadas E,Muñoz P,Bouza E

    更新日期：2020-07-01 00:00:00

  • Rapid methods in clinical microbiology. Clinical situations in which rapid methods may best be applied.

    abstract：:Utilization of rapid microbiologic methods could improve both the quality of patient care and help to reduce patient health care costs through more efficient patient management. Medically urgent situations such as meningitis, endocarditis, bacteremia, some soft tissue infections, ocular infections, and pneumonia in im...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(85)90047-1

    authors： Jorgensen JH

    更新日期：1985-11-01 00:00:00

  • Vertical transmission of human immunodeficiency virus type 1. Prognostic value of IgA antibody to HIV-1 polypeptides during pregnancy.

    abstract：:In a retrospective study of 31 pregnant women infected with human immunodeficiency virus type 1 (HIV-1), nine (29%) infants presented unequivocal signs of HIV-1 infection (persistent p24 antigenemia and/or positive virus isolation). All serum samples obtained from the others, during pregnancy and on delivery, were stu...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90108-6

    authors： Re MC,Furlini G,Vignoli M,Ricchi E,Ramazzotti E,Bianchi S,Guerra B,Costigliola P,La Placa M

    更新日期：1992-08-01 00:00:00

  • Mycetoma of the scalp due to Microsporum canis: hystopathologic, mycologic, and immunogenetic features in a 6-year-old girl.

    abstract：:Dermatophytic mycetoma is an extremely rare subcutaneous mycosis. Here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by Microsporum canis. To our knowledge, this is the first report showing the immunologic and immunogenetic fea...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.02.003

    authors： Chiapello LS,Dib MD,Nuncira CT,Nardelli L,Vullo C,Collino C,Abiega C,Cortes PR,Spesso MF,Masih DT

    更新日期：2011-05-01 00:00:00