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Performance of a molecular viability assay for the diagnosis of Pneumocystis pneumonia in HIV-infected patients.

Abstract：

:Pneumocystis pneumonia (PCP), caused by infection with Pneumocystis jirovecii, remains an important opportunistic infection in humans. A reverse transcriptase polymerase chain reaction assay has been shown to specifically detect viable P. jirovecii organisms. In the current study, we evaluated this assay on different types of respiratory samples. The assay had a diagnostic sensitivity of 100% and a specificity of 86% when applied to bronchoalveolar lavage samples. The assay's performance declined when applied to less invasive induced sputum and oropharyngeal wash (OPW) samples. The sensitivity, when applied to OPWs, was improved by examining multiple sequential OPW samples and was affected by clinical sampling parameters that could increase or decrease the number of potential organisms in the oropharynx. When used in conjunction with an optimized clinical sampling protocol, this assay may become a useful tool for detecting and monitoring P. jirovecii in minimally invasive clinical samples.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

de Oliveira A,Unnasch TR,Crothers K,Eiser S,Zucchi P,Moir J,Beard CB,Lawrence GG,Huang L

doi

10.1016/j.diagmicrobio.2006.08.015

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

169-76

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(06)00327-0

journal_volume

57

pub_type

杂志文章

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