Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice.

Abstract:

:Coxiella burnetii, a Gram-negative intracellular bacterium, can give rise to Q fever in humans and is transmitted mainly by inhalation of infected aerosols from animal reservoirs. Serology is commonly used to diagnose Q fever, but the early cellular immune response-i.e., C. burnetii-specific interferon γ (IFN-γ) production in response to antigen challenge-might be an additional diagnostic. Detection of IFN-γ responses has been used to identify past and chronic Q fever infections, but the IFN-γ response in acute Q fever has not been described. By challenging immunocompetent BALB/c mice with aerosols containing phase I C. burnetii, the timing and extent of IFN-γ recall responses were evaluated in an acute C. burnetii infection. Other cytokines were also measured in an effort to identify other potential diagnostic markers. The data show that after initial expansion of bacteria first in lungs and then in other tissues, the infection was cleared from day 10 onwards as reflected by the decreasing number of bacteria. The antigen-induced IFN-γ production by splenocytes coincided with emergence of IgM phase II antibodies at day 10 postinfection and preceded appearance of IgG antibodies. This was accompanied by the production of proinflammatory cytokines including interleukin (IL) 6, keratinocyte-derived cytokine, and IFN-γ-induced protein 10, followed by monocyte chemotactic protein 1, but not by IL-1β and tumor necrosis factor α, and only very low production of the anti-inflammatory cytokine IL-10. These data suggest that analysis of antigen-specific IFN-γ responses could be a useful tool for diagnosis of acute Q fever. Moreover, the current model of C. burnetii infection could be used to give new insights into immunological factors that predispose to development of persistent infection.

authors

Schoffelen T,Self JS,Fitzpatrick KA,Netea MG,van Deuren M,Joosten LA,Kersh GJ

doi

10.1016/j.diagmicrobio.2014.12.008

subject

Has Abstract

pub_date

2015-04-01 00:00:00

pages

234-9

issue

4

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(14)00518-5

journal_volume

81

pub_type

杂志文章
  • Evaluation of the Abbott ARCHITECT Toxo IgM assay.

    abstract::Development of the ARCHITECT Toxo IgM assay has been done to assist the clinician in acute Toxoplasma gondii infection detection, especially in pregnant women. Its use, in conjunction with ARCHITECT Toxo IgG and Toxo Avidity assays, will provide an array of assays particularly useful in the monitoring of pregnant fema...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2009.03.016

    authors: Sickinger E,Braun HB,Praast G,Stieler M,Gundlach C,Birkenbach C,Prostko J,Palafox MA,Frias E,Hsu S,Matias M,Pucci D,Hausmann M,Sagel U,Smith D

    更新日期:2009-07-01 00:00:00

  • Hepatitis C virus core antigen in the management of patients treated with new direct-acting antivirals.

    abstract::We evaluated the utility of Architect core antigen assay® Abbott Diagnostics (HCVAg) for monitoring patients with HCV infection and compared to HCV-RNA quantification (Cobas Ampliprep TaqMan-Roche Diagnostics). Samples from 262 patients were studied. Mean baseline HCV RNA and HCVAg levels were similar for responders (...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.06.006

    authors: Arboledas JCA,Guerrero IP,Rodríguez MJB,Martos ET,Pérez AB,León CC,Sierra Sánchez JF,Prieto MDL,Porcuna NC,Mochón MDO,Macías J,de la Iglesia Salgado A,Granger JR,Fernández MD,Lozano IG,Ramírez ER,Rivero A,Del Carmen Loz

    更新日期:2017-09-01 00:00:00

  • Direct detection of Streptococcus pneumoniae in positive blood cultures by real-time polymerase chain reaction.

    abstract::We developed a real-time polymerase chain reaction specific for Streptococcus pneumoniae to be applied directly from blood culture bottles without previous DNA extraction step. For the 128 blood culture bottles tested, the assay had 94% and 98.4% sensitivity and specificity, respectively. This assay provides rapid and...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2009.05.023

    authors: Selva L,Esteva C,Gené A,de Sevilla MF,Hernandez-Bou S,Muñoz-Almagro C

    更新日期:2010-02-01 00:00:00

  • Increased bacterial adherence and biomass in Pseudomonas aeruginosa bacteria exposed to clarithromycin.

    abstract::Long-term low-dose macrolides alter response in patients with chronic sessile Pseudomonas aeruginosa colonization. We examined the effect of clarithromycin on 1) adherence of P. aeruginosa cells and 2) biofilm formation. A suspended-coupon continuous-flow biofilm reactor model was used. Adherent P. aeruginosa bacteria...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2008.09.007

    authors: Garey KW,Vo QP,Lewis RE,Saengcharoen W,LaRocco MT,Tam VH

    更新日期:2009-01-01 00:00:00

  • Vertical transmission of human immunodeficiency virus type 1. Prognostic value of IgA antibody to HIV-1 polypeptides during pregnancy.

    abstract::In a retrospective study of 31 pregnant women infected with human immunodeficiency virus type 1 (HIV-1), nine (29%) infants presented unequivocal signs of HIV-1 infection (persistent p24 antigenemia and/or positive virus isolation). All serum samples obtained from the others, during pregnancy and on delivery, were stu...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90108-6

    authors: Re MC,Furlini G,Vignoli M,Ricchi E,Ramazzotti E,Bianchi S,Guerra B,Costigliola P,La Placa M

    更新日期:1992-08-01 00:00:00

  • Evaluation of Brucella dipstick assay for the diagnosis of acute brucellosis.

    abstract::The diagnostic value of the dipstick assay was evaluated by comparison with Rose Bengal (RB), serum aglutination tests (SAT) and 2 mercaptoethanol test (2-ME) on consecutive serum samples submitted because of suspicion of brucellosis. Serum samples of 232 patients with suspected brucellosis that were submitted for lab...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00456-x

    authors: Altuglu I,Zeytinoğlu A,Bilgic A,Kamcioglu S,Karakartal G,Smits H

    更新日期:2002-11-01 00:00:00

  • In vitro activity of lomefloxacin against multiply resistant Pseudomonas aeruginosa, Enterobacter cloacae, and Staphylococcus epidermidis.

    abstract::Lomefloxacin is a promising new difluorinated quinolone with good antimicrobial activity and favorable pharmacokinetic properties. The in vitro activity of lomefloxacin was compared to other quinolones, expanded spectrum beta-lactams, and gentamicin. The lomefloxacin MIC90 against multiply resistant strains of P. aeru...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(89)90059-x

    authors: DiVincenzo CA,Shatzer KL,Venezio FR

    更新日期:1989-05-01 00:00:00

  • Clinical features and in vitro susceptibilities of two varieties of Cryptococcus neoformans in Taiwan.

    abstract::One hundred years after introduction of the Eucalyptus tree to Taiwan, a predominantly subtropical island, we analyzed clinical and microbiological data of 59 patients with Cryptococcus neoformans infection hospitalized at National Taiwan University Hospital during 1982 to 1997. There were 38 (64.4%) cases of cryptoco...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(99)00137-6

    authors: Chen YC,Chang SC,Shih CC,Hung CC,Luhbd KT,Pan YS,Hsieh WC

    更新日期:2000-03-01 00:00:00

  • Use of surrogate antimicrobial agents to predict susceptibility to ertapenem.

    abstract::Broth or agar dilution susceptibility test results for Enterobacteriaceae (11,775 strains), anaerobes (2888 strains), staphylococci (2206 strains), Haemophilus spp. (840 strains), group A streptococci (280 strains), group B streptococci (269 strains), Streptococcus pneumoniae (709 strains), and 160 other streptococci ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00380-2

    authors: Friedland IR,Isaacs R,Mixson LA,Motyl M,Woods GL

    更新日期:2002-05-01 00:00:00

  • Clinical characteristics of bacteremia caused by hypermucoviscous Klebsiella pneumoniae at a tertiary hospital.

    abstract::This study aimed to assess the predictive factors of bacteremia due to hypermucoviscous Klebsiella pneumoniae (hvKP), as well as the mortality. The medical records of 114 patients with K. pneumoniae bacteremia who were divided into the hvKP (n = 24) and non-hvKP (n = 90) groups and were retrospectively reviewed. The m...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2019.04.008

    authors: Namikawa H,Yamada K,Sakiyama A,Imoto W,Yamairi K,Shibata W,Yoshii N,Niki M,Nakaie K,Oinuma KI,Tsubouchi T,Niki M,Tochino Y,Takemoto Y,Kaneko Y,Shuto T,Kakeya H

    更新日期:2019-09-01 00:00:00

  • Vertebral osteomyelitis: nontypeable beta-lactamase-negative Haemophilus influenzae in an adult: case report.

    abstract::Adult pyogenic vertebral osteomyelitis due to Haemophilus influenzae is exceedingly rare. After a search of the literature, we deemed our case to be the seventh case of H. influenzae pyogenic osteomyelitis. Vertebral osteomyelitis in itself is a rarity. The most common organisms associated with vertebral osteomyelitis...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/s0732-8893(97)00073-4

    authors: Personius CD,Camp CJ

    更新日期:1997-08-01 00:00:00

  • Diarrhoeagenic Escherichia coli--an emerging problem?

    abstract::Diarrhea remains one of the main sources of morbidity and morbidity in today's world and a large proportion is caused by diarrheagenic Escherichia coli. They are a particular problem in developed countries although traveler's diarrhea and hemorrhagic colitis are also a problem in developed countries. There are seven c...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/s0732-8893(01)00303-0

    authors: Clarke SC

    更新日期:2001-11-01 00:00:00

  • In vitro activity of oritavancin against planktonic and biofilm states of vancomycin-susceptible and vancomycin-resistant enterococci.

    abstract::We tested the in vitro activity of oritavancin against 60 vancomycin-susceptible enterococci (VSE) and 27 vancomycin-resistant enterococci (VRE). The oritavancin MIC ranged from ≤0.002 to 0.5μg/mL; the minimum biofilm bactericidal concentration ranged from ≤0.002 to 2μg/mL. Oritavancin has promising in vitro activity ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.03.008

    authors: Yan Q,Karau MJ,Patel R

    更新日期:2018-08-01 00:00:00

  • In vitro pharmacodynamic activity of gatifloxacin, gemifloxacin, moxifloxacin and levofloxacin against Streptococcus pneumoniae containing specific mutations in DNA gyrase and topoisomerase IV.

    abstract::An in vitro pharmacodynatnic modeling apparatus (PDMA) generated specific bacterial kill profiles for single-dose regimens of gatifloxacin (GT), gemifloxacin (GM), moxifloxacin (MX) and levofloxacin (LV) against isolates of Streptococcus pneumoniae with specific QRDR profiles: SP-WT (no modifications); SP-C (changes i...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(03)00152-4

    authors: Garrison MW,Schimmels JA,Madaras-Kelly KJ

    更新日期:2003-12-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract::This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.08.006

    authors: Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期:2008-02-01 00:00:00

  • Piperacillin/tazobactam versus imipenem: a double-blind, randomized formulary feasibility study at a major teaching hospital.

    abstract::With the introduction of piperacillin/tazobactam to the North American market, hospitals have been faced with the task of making a decision regarding its formulary role. In view of its broad spectrum of activity, piperacillin/tazobactam could be considered as a formulary alternative to imipenem. To evaluate the formul...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/s0732-8893(97)00239-3

    authors: Marra F,Reynolds R,Stiver G,Bryce E,Sleigh K,Frighetto L,MacDougall C,Jewesson P

    更新日期:1998-06-01 00:00:00

  • Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers.

    abstract::Rapid and accurate diagnostic tests for Clostridium difficile infections (CDI) are crucial for management of patients with suspected CDI and for infection control. Enzyme immunoassays for detection of the toxins are routinely used but lack adequate sensitivity. We generated slow off-rate modified aptamers (SOMAmer™ re...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.03.029

    authors: Ochsner UA,Katilius E,Janjic N

    更新日期:2013-07-01 00:00:00

  • Antimicrobial activity of cefepime tested against Bush group I beta-lactamase-producing strains resistant to ceftazidime. A multilaboratory national and international clinical isolate study.

    abstract::The potency of cefepime, a parenteral aminothiazolyl methoxyimino cephalosporin, was assessed against 256 ceftazidime-resistant Gram-negative bacilli from five medical centers in the United States. In addition, cefepime activity was compared with that of ciprofloxacin and imipenem against 506 ceftazidime-resistant Gra...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,多中心研究

    doi:10.1016/0732-8893(94)90048-5

    authors: Jones RN,Marshall SA

    更新日期:1994-05-01 00:00:00

  • Carry-over of host nutrients during sampling enhances undesired growth of Staphylococcus aureus in liquid Amies transport medium.

    abstract::Optimal transportation of bacteria is important for accurate clinical interpretation, quantitative assays, and microbiome studies. A transport medium should ideally keep the bacteria alive without supporting growth or altering the relative proportions of the constituent species. We investigated the effect of nasal muc...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.07.009

    authors: Islam MZ,Larsen J,Skov R,Angen Ø

    更新日期:2019-01-01 00:00:00

  • Comparative in vitro activity of cefoxitin, cefotaxime alone, and in combination with desacetylcefotaxime against the Bacteroides species.

    abstract::The agar dilution method was used to determine the inhibitory activity of cefotaxime (CTX) alone, desacetylcefotaxime (dCTX) alone, CTX plus dCTX, and cefoxitin against 74 clinical isolates of the Bacteroides species recovered from diabetic patients with foot ulcers. The study concluded that the addition of dCTX to CT...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(89)90043-6

    authors: Canawati HN

    更新日期:1989-01-01 00:00:00

  • Application of cetylpyridinium chloride and sodium chloride decontamination method for recovery of Mycobacterium tuberculosis from clinically suspected cases of pulmonary tuberculosis.

    abstract::The study was designed to compare the efficacy of cetylpyridinium chloride (CPC) and sodium chloride (NaCl) decontamination method with N-acetyl L-Cystine (NALC) and sodium hydroxide (NaOH) decontamination (the reference method) method for the recovery of Mycobacterium tuberculosis (MTB) from clinically suspected case...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.06.021

    authors: Shinu P,Singh V,Nair A,Mehrishi P,Mehta S,Joshi E

    更新日期:2013-10-01 00:00:00

  • Evaluation of a commercial latex agglutination method for toxoplasmosis.

    abstract::A commercial latex agglutination test for detecting immunoglobulin G antibody to Toxoplasma gondii, the Syn-Kit Toxotest MT, was evaluated in two laboratories against the Sabin-Feldman dye test, the indirect immunofluorescence test, and FIAX. The Toxotest MT was found easy to perform and required no special equipment ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(83)90001-9

    authors: Walls KW,Remington JS

    更新日期:1983-12-01 00:00:00

  • Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus.

    abstract::During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of d...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.05.004

    authors: Dauner AL,Mitra I,Gilliland T Jr,Seales S,Pal S,Yang SC,Guevara C,Chen JH,Liu YC,Kochel TJ,Wu SJ

    更新日期:2015-09-01 00:00:00

  • Cultures of sternal wound and mediastinum taken at the end of heart surgery do not predict postsurgical mediastinitis.

    abstract::The aim of the study was to assess of the role of intraoperative cultures taken at the end of major heart surgery (MHS) in the prediction of postoperative mediastinitis (PM) in patients undergoing MHS over a 6-month period in a tertiary university hospital. Just before wound closure, a sample of the sternal border was...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.06.012

    authors: Bouza E,Muñoz P,Alcalá L,Pérez MJ,Rincón C,Barrio JM,Pinto A

    更新日期:2006-12-01 00:00:00

  • In vitro activity of l-ofloxacin against norfloxacin-resistant coagulase-negative staphylococci.

    abstract::The in vitro activity of l-ofloxacin was determined against coagulase-negative staphylococci that were induced to norfloxacin resistance. l-Ofloxacin was the most active agent tested with an MIC90 of 4 micrograms/ml compared with greater than 128, 32, and 128 micrograms/ml for norfloxacin, ciprofloxacin, and enoxacin,...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90109-7

    authors: Foleno B,Fu KP

    更新日期:1992-08-01 00:00:00

  • WITHDRAWN: Selection of a surrogate beta-lactam testing agent for initial susceptibility testing of doripenem, a new carbapenem.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.09.009

    authors: Jones RN,Sader HS,Fritsche TR,Janechek MJ

    更新日期:2007-10-04 00:00:00

  • Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples.

    abstract::We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.01.013

    authors: Huang W,Wang G,Lin H,Zhuge J,Nolan SM,Vail E,Dimitrova N,Fallon JT

    更新日期:2016-05-01 00:00:00

  • Mucorales DNA detection in serum specimens for early diagnosis of mucormycosis.

    abstract::We report a case of pulmonary mucormycosis in a patient with T-cell acute lymphoblastic leukemia. The diagnosis of mucormycosis was initially based on mycological examination of a pulmonary specimen. However, we describe how it could have been made 2 months earlier using polymerase chain reaction assays targeting Muco...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type:

    doi:10.1016/j.diagmicrobio.2020.115004

    authors: Hoffmann C,Guillerm G,Le Pape P,Carausu L,Lavergne RA,Nevez G,Le Gal S

    更新日期:2020-06-01 00:00:00

  • Extended spectrum beta-lactamase production and fluorquinolone resistance in pathogens associated with community acquired urinary tract infection.

    abstract::We have evaluated the susceptibility of 199 pathogens isolated in pure culture from consecutive urine samples submitted from the community. Rates of susceptibility for all organisms were ampicillin, 48%; amoxycillin/clavulanic acid, 88%; cephalothin, 57%; cefuroxime axetil, 74%; nalidixic acid, 85%; ciprofloxacin, 99%...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(98)00113-8

    authors: Cormican M,Morris D,Corbett-Feeeney G,Flynn J

    更新日期:1998-12-01 00:00:00

  • Bipolaris oryzae, a novel fungal opportunist causing keratitis.

    abstract::We report a case of mycotic keratitis caused by Bipolaris oryzae with predisposing trauma from a foreign body. The fungus was identified by sequencing the internal transcribed spacer region, translation elongation factor 1α (TEF1) gene, and partial glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene, and the species ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.11.020

    authors: Wang L,Al-Hatmi AM,Lai X,Peng L,Yang C,Lai H,Li J,Meis JF,de Hoog GS,Zhuo C,Chen M

    更新日期:2016-05-01 00:00:00