Abstract:
:Between February 2005 and January 2006 in Srinagarind Hospital, Thailand, 44 from 1730 isolates (2.5%) of Escherichia coli and 8 from 982 isolates (0.8%) of Klebsiella pneumoniae were found to produce plasmid-mediated AmpC β-lactamases (pAmpCs) as detected by a cefoxitin-Hodge test followed by a multiplex polymerase chain reaction (PCR) technique. Fifteen of the 52 pAmpC-producing isolates also produced extended-spectrum β-lactamases. The ampC genes found in both organisms were bla(CMY-2) (46 isolates), bla(CMY-8b) (4 isolates), and bla(DHA-1) (2 isolates). These genes were present on plasmids. Twenty-five of the 46 CMY-2-producing isolates could transfer cefoxitin resistance to E. coli UB1637 by conjugation. More than 90% of the pAmpC-producing isolates were resistant to cefoxitin, but 80% to 90% of them were susceptible or intermediately susceptible to ceftazidime or cefotaxime. Enterobacterial repetitive intergenic consensus PCR analysis revealed that most isolates were of different strains, indicating the ease of transmission of these resistance determinants. This is the first report of CMY-2, CMY-8b, and DHA-1 β-lactamases in Thai isolates.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Singtohin S,Chanawong A,Lulitanond A,Sribenjalux P,Auncharoen A,Kaewkes W,Songsri J,Pienthaweechai Kdoi
10.1016/j.diagmicrobio.2010.06.014subject
Has Abstractpub_date
2010-11-01 00:00:00pages
271-7issue
3eissn
0732-8893issn
1879-0070pii
S0732-8893(10)00242-7journal_volume
68pub_type
杂志文章abstract::MB-Redox is a new manual culture system designed for the recovery of mycobacteria from clinical specimens. It consists of a liquid medium (modified Kirchner medium) containing a redox indicator, a colorless tetrazolium salt, which is reduced to colored formazan by actively growing mycobacteria. Acid fast bacilli (AFB)...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,多中心研究
doi:10.1016/s0732-8893(99)00029-2
更新日期:1999-08-01 00:00:00
abstract::The in vitro activity of ampicillin, BMY 28142, and imipenem was evaluated against 21 clinical isolates of Mycobacterium avium complex by both a broth and an agar dilution method. The MIC90 by broth dilution for ampicillin, BMY 28142, and imipenem was 16 micrograms/ml, 8 micrograms/ml, and greater than 32 micrograms/m...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(87)90100-3
更新日期:1987-02-01 00:00:00
abstract::Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = 1). PCR and DNA sequence analysis reveale...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2013.12.003
更新日期:2014-03-01 00:00:00
abstract::The in vitro susceptibility of 19 antipseudomonal antibiotics, old and new, were tested against 100 blood isolates of Pseudomonas aeruginosa. The isolates were taken from consecutive bacteremic patients hospitalized in nine Pittsburgh hospitals from 1983 to 1984. Twelve percent of isolates tested were tobramycin-resis...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(87)90027-7
更新日期:1987-06-01 00:00:00
abstract::The aim of the study was to evaluate the influence of dietary and demographic factors and some habits on the prevalence of Helicobacter pylori infection in 150 dyspeptic patients examined endoscopically and by the urea breath test. Positivity rate was lower (50.6%) in patients consuming honey ≥1 day weekly compared wi...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2015.03.001
更新日期:2015-05-01 00:00:00
abstract::Respiratory syncytial virus (RSV) often causes respiratory illness in adults. Over 40 RSV vaccine and monoclonal antibody products are currently in preclinical development or clinical trials. Because RSV diagnostic practices may impact disease burden estimates, we investigated infectious disease physicians' RSV diagno...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2017.12.011
更新日期:2018-11-01 00:00:00
abstract::The emergence and sustained prevalence of Gram-positive organisms resistant to antimicrobials has been of interest for over a decade. Quinupristin/dalfopristin (formerly RP 59500 or Synercid) is a new injectable streptogramin combination that has been reported to have activity against Gram-positive organisms, even tho...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(00)00111-5
更新日期:2000-04-01 00:00:00
abstract::Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsil...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2011.07.002
更新日期:2011-11-01 00:00:00
abstract::Cefotaxime, the first widely used "third-generation" cephalosporin, has established efficacy against a variety of serious bacterial pathogens. Some of the initial clinical studies in the United States using this agent employed large doses of the compound, up to 12 g/day, for adults. In contrast, however, initial Europ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,评审
doi:10.1016/0732-8893(95)00103-h
更新日期:1995-05-01 00:00:00
abstract::The rose bengal test is often used for rapid diagnosis of human brucellosis in endemic areas. However, autoantibodies have never been investigated as a reason for false-positive or false-negative results. Therefore, the aim of this study was to show the effect of autoantibody detection on the rapid diagnosis of human ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2007.01.003
更新日期:2007-07-01 00:00:00
abstract::The aim of the study was to assess of the role of intraoperative cultures taken at the end of major heart surgery (MHS) in the prediction of postoperative mediastinitis (PM) in patients undergoing MHS over a 6-month period in a tertiary university hospital. Just before wound closure, a sample of the sternal border was...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2006.06.012
更新日期:2006-12-01 00:00:00
abstract::The performance of peptide-, recombinant protein-, and whole virus lysate-based enzyme immunosorbent assays (EIAs) for the detection of antibodies to the human immunodeficiency virus type 1 (HIV-1) was compared on a panel of 245 routine samples, a panel of low-positive samples, four seroconversion panels, and serial d...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(93)90085-l
更新日期:1993-05-01 00:00:00
abstract::Development of a new phenotypic technique for rapid antimicrobial susceptibility testing (AST) of methicillin-resistant Staphylococcus aureus is presented. The new technique combines bacterial culturing and specific immunometric detection in a single separation-free process. The technique uses dry chemistry reagents a...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2008.07.007
更新日期:2008-11-01 00:00:00
abstract::Adult patients with hematologic malignancies along with HIV infected patients were prospectively studied to determine the performance of urine D-arabinitol/L-arabinitol (DA/LA) ratio in diagnosing invasive candidiasis. Ten evaluable febrile neutropenic patients had proven invasive candidiasis and elevated DA/LA ratios...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(01)00310-8
更新日期:2002-01-01 00:00:00
abstract::Corynebacterium jeikeium is the name recently given to a group of coryneform rods, formerly known as the JK group, and associated with a wide range of serious infections. We describe five patients with C. jeikeium endocarditis observed in two Belgian hospitals over a period of 5 years. In three cases, the infection oc...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(89)90025-4
更新日期:1989-05-01 00:00:00
abstract::Damage to the intestinal mucosa by Clostridium difficile (CD) is toxin mediated. Two enzyme immunoassays (EIAs) for toxin-A detection, the automated Vitek immunodiagnostic assay system CDA (Vidas CDA), and the Premier toxin A (Premier) were tested for their ability to detect toxin A in 301 stool samples and compared w...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(93)90062-c
更新日期:1993-07-01 00:00:00
abstract::Our aim was to assess growth effects of adrenaline on 32 Helicobacter pylori strains on 2.5% blood agar. By agar-well diffusion and disk diffusion methods, concentration-dependent growth stimulation occurred in ≤50.0% and 36.8% of the strains, respectively. Growth inhibition was less frequent. Most strains were adrena...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2018.08.004
更新日期:2019-01-01 00:00:00
abstract::Biomarkers such as procalcitonin (PCT) have been studied to guide duration of antibiotic therapy. We aimed to assess whether a decrease in PCT levels could be used to reduce the duration of antibiotic therapy in intensive care unit (ICU) patients with a proven infection without risking a worse outcome. We assessed 265...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.diagmicrobio.2013.03.027
更新日期:2013-07-01 00:00:00
abstract::Sixty-three cases of Streptococcus pyogenes meningitis in adults were studied. Three predominant emm types were associated with meningitis: emm1 (44%), emm3 (11%), and emm6 (11%). Streptococcal toxic shock syndrome and mortality rates were 40% and 38%, respectively. ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2015.12.006
更新日期:2016-04-01 00:00:00
abstract::Tigecycline is a new first-in-class glycylcycline antimicrobial agent with expanded broad-spectrum activity against both Gram-negative and Gram-positive aerobes and anaerobes, as well as atypical bacterial species. The spectrum of activity extends to clinically relevant susceptible and multidrug-resistant strains of S...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,评审
doi:10.1016/j.diagmicrobio.2005.05.006
更新日期:2005-07-01 00:00:00
abstract::There are no studies evaluating the mechanisms driving linezolid resistance in nontuberculous mycobacteria. The novel mutations G2599A and A2137T in the 23S rRNA gene and mutations A439G and G443A in the rplD gene associated with linezolid resistance were identified in linezolid-resistant M. avium complex isolates. ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2018.10.022
更新日期:2019-05-01 00:00:00
abstract::An antimicrobial resistance surveillance study in Japan is presented representing the second year (Phase II) results from 22 medical centers. Each participant laboratory tested (Etest, AB BIODISK, Solna, Sweden) 100 organisms, 10 strains each from 10 species groups including Escherichia coli, Klebsiella spp., Enteroba...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 临床试验,杂志文章
doi:10.1016/s0732-8893(99)00120-0
更新日期:1999-12-01 00:00:00
abstract::The application of typing procedures for the purpose of strain differentiation among isolates of Candida albicans obtained from hospitalized patients has been limited. We have applied biotyping and DNA restriction fragment analysis (DNA fingerprinting) by using EcoRI to the study of C. albicans isolates obtained from ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(90)90080-f
更新日期:1990-11-01 00:00:00
abstract::Fosfomycin and comparators were susceptibility tested against over 2200 contemporary clinical isolates from US medical centers. Fosfomycin was active against Enterobacterales (MIC50/90, 4/16 μg/mL), including multidrug-resistant isolates. Potent activity was exhibited against gram-positive organisms, including Staphyl...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2018.08.010
更新日期:2019-02-01 00:00:00
abstract::We evaluate sequence data from the PathChip high-density hybridization array for epidemiological interpretation of detected pathogens. For influenza A, we derive similar relative outbreak clustering in phylogenetic trees from PathChip-derived compared to classical Sanger-derived sequences. For a positive polio detecti...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2015.11.003
更新日期:2016-03-01 00:00:00
abstract::To explore a simple, rapid, and inexpensive way to identify Mycobacterium tuberculosis complex culture, dot blot hybridization using IS6110 as the marker was performed against 2788 known clinical isolates of mycobacteria including M. tuberculosis (n = 721), M. kansasii (177), M. marinum (10), M. avium complex (700), M...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(98)00136-9
更新日期:1999-01-01 00:00:00
abstract::We described 27 polyclonal colistin-resistant Enterobacteriaceae (MIC 4-16 μg/mL) infections (12 pneumonia, 12 urinary tract infection (UTI), two Bacteremia, and one skin/soft tissue infection) in which 74% harbored KPC. The isolates were polyclonal, 6 STs were identified and the colistin resistance was due to chromos...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2016.11.007
更新日期:2017-03-01 00:00:00
abstract::Recurrence is a common complication of Staphylococcus aureus infections. A shorter duration of antibiotic treatment for a S. aureus infection has been previously suggested as a possible risk factor for recurrence. We conducted a retrospective cohort study of patients with S. aureus infection complicated by bacteremia ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2006.01.021
更新日期:2006-07-01 00:00:00
abstract::Cycling Probe Technology (CPT) was combined with a colorimetric enzyme-immuno assay (EIA) to develop two assays for the detection of vanA and vanB genes in vancomycin resistant enterococci (VRE). The CPT-EIA assay employs a gene-specific fluorescein labeled DNA-RNA-DNA probe that gets cleaved within the probe : target...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(99)00149-2
更新日期:2000-05-01 00:00:00
abstract::Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples. ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2015.12.017
更新日期:2016-06-01 00:00:00