当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > A rapid, standardized protein extraction method using adaptive focused acoustics for identification of mycobacteria by MALDI-ToF MS.

A rapid, standardized protein extraction method using adaptive focused acoustics for identification of mycobacteria by MALDI-ToF MS.

Abstract：

:Mycobacterial identification using MALDI-ToF MS (MALDI) has been hindered by inadequate extraction methods. Adaptive Focused Acoustics™ uses concentrated ultrasonic energy to achieve cellular disruption. Using this technology, we developed a rapid mycobacterial inactivation/protein extraction method for MALDI-based identification. Agreement for identification to the species level versus conventional identification was stratified by log confidence cut-offs of ≥2.0, ≥ 1.8, or ≥1.7. A total of 182 mycobacterial isolates were tested. Complete inactivation of all species/strains was achieved after 2min. Using a log confidence cut-off of ≥2.0, overall agreement for the commercial method (CM) was 41.7% versus 66.7% for the novel method (NM). For the CM, agreement increased to 66.7% and 83.3% using log confidence cut-offs of ≥1.8 and ≥1.7, respectively; for the NM, agreement was 100% for both cut-offs with all isolates. With no alteration to the existing database, overall agreement for the NM was 83.4%, largely due to low scores for clinical isolates of M. chelonae and M. mucogenicum. Addition of spectra from a single clinical strain of each species to the existing database increased overall agreement to 93.1%.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Adams LL,Dionne K,Fisher S,Parrish N

doi

10.1016/j.diagmicrobio.2016.06.001

subject

Has Abstract

pub_date

2016-11-01 00:00:00

pages

284-288

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(16)30161-4

journal_volume

86

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Identification of genetic recombination between Acinetobacter species based on multilocus sequence analysis.

    abstract：:During multilocus sequence analysis of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 Acinetobacter species. While 3 isolates belonged to A. nosocomialis based on 16S rRNA, gyrB, fusA, gdhB, and rplB gene sequences, they showed close re...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.03.027

    authors： Kim DH,Park YK,Choi JY,Ko KS

    更新日期：2012-07-01 00:00:00

  • Enterobacterial repetitive intergenic consensus PCR is a useful tool for typing Mycobacterium chelonae and Mycobacterium abscessus isolates.

    abstract：:Outbreaks of rapidly growing mycobacterium (RGM) infections are increasingly being reported worldwide. Information about genetic relatedness of isolates obtained during outbreaks can provide opportunities for prompt intervention. Pulsed-field gel electrophoresis (PFGE) is expensive, time consuming, and labor intensive...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.01.006

    authors： Sampaio JL,Viana-Niero C,de Freitas D,Höfling-Lima AL,Leão SC

    更新日期：2006-06-01 00:00:00

  • Gatifloxacin in community-based treatment of acute respiratory tract infections in the elderly.

    abstract：:The elderly are at increased risk for respiratory tract infections. To evaluate the safety and efficacy of gatifloxacin in adults of any age with community-acquired respiratory tract infections, this open-label, multicenter, noncomparative study in community-based practices enrolled male and female outpatients at leas...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章,多中心研究

    doi：10.1016/s0732-8893(02)00458-3

    authors： Nicholson SC,High KP,Gothelf S,Webb CD

    更新日期：2002-09-01 00:00:00

  • Serological response to plasmid-encoded antigens in children and adults with shigellosis.

    abstract：:The immunological response to plasmid-encoded antigens of virulent Shigella was determined in Thai children less than 4 yr of age and in Thai adults by immunoblot analysis and ELISA. Forty-two percent (8/19) of Thai children and 4% (1/22) of Thai adults with shigellosis developed a greater than or equal to 4-fold rise...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(88)90043-0

    authors： Echeverria P,Hanchalay S,Taylor DN

    更新日期：1988-06-01 00:00:00

  • Comparison of two oral antibiotics, roxithromycin and amoxicillin plus clavulanic acid, in lower respiratory tract infections.

    abstract：:In a randomized, multicenter, open-label study, 490 ambulatory adult patients with lower respiratory tract infection (LRTI) were randomized to roxithromycin (ROX) 150 mg b.i.d. orally (n = 244) or amoxicillin plus clavulanic acid (AMX + CA) as 500 mg AMX + 125 mg CA t.i.d orally (n = 24). Clinical results were analyze...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章,多中心研究,随机对照试验

    doi：10.1016/0732-8893(92)90133-e

    authors： Dautzenberg B,Scheimberg A,Brambilla C,Camus P,Godard P,Guerin JC,Lemarie E,Rezvani Y,Rosembaum M,Tuchais E

    更新日期：1992-05-01 00:00:00

  • Epidemiology of macrolide and/or lincosamide resistant Streptococcus pneumoniae clinical isolates with ribosomal mutations.

    abstract：:Twenty macrolide and/or lincosamide resistant Streptococcus pneumoniae clinical isolates from various sources with 50S ribosomal mutations were identified. Mutations were identified in the 23S rDNA with substitutions at A2058, A2059, or C2611 and in L4 or L22 ribosomal protein genes. Fourteen were A2059G substitutions...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/S0732-8893(03)00130-5

    authors： Doktor SZ,Shortridge VD,Beyer JM,Flamm RK

    更新日期：2004-05-01 00:00:00

  • Evaluation of a novel chromogenic medium for Candida spp. identification and comparison with CHROMagar™ Candida for the detection of Candida auris in surveillance samples.

    abstract：:A shift to Candida non-albicans infections has been noted during the last years, and the emergence of multi-resistant Candida auris has complicated their management. The aim of this study was first to compare the performance of the novel chromogenic medium CHROMagar™ Candida Plus (CHROMagar, France) with CHROMagar™ Ca...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115168

    authors： Mulet Bayona JV,Salvador García C,Tormo Palop N,Gimeno Cardona C

    更新日期：2020-12-01 00:00:00

  • Risk factors for recurrence in patients with Staphylococcus aureus infections complicated by bacteremia.

    abstract：:Recurrence is a common complication of Staphylococcus aureus infections. A shorter duration of antibiotic treatment for a S. aureus infection has been previously suggested as a possible risk factor for recurrence. We conducted a retrospective cohort study of patients with S. aureus infection complicated by bacteremia ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.01.021

    authors： Kreisel K,Boyd K,Langenberg P,Roghmann MC

    更新日期：2006-07-01 00:00:00

  • Candidemia due to Candida tropicalis: clinical, epidemiologic, and microbiologic characteristics of 188 episodes occurring in tertiary care hospitals.

    abstract：:Candida tropicalis is the 2nd most frequent agent of candidemia in Brazil (20-24%). We attempted to characterize the epidemiology, microbiology, and outcome of candidemia due to C. tropicalis by comparing patients with candidemia due to C. tropicalis with those with candidemia due to Candida albicans. Among the 924 ep...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.11.009

    authors： Nucci M,Colombo AL

    更新日期：2007-05-01 00:00:00

  • Pharmacokinetics-pharmacodynamics, computer decision support technologies, and antimicrobial stewardship: the compass and rudder.

    abstract：:The first guidelines for conducting antimicrobial stewardship in the hospitalized setting were published in 2007. These guidelines recommend that stewardship programs employ the science of pharmacokinetics-pharmacodynamics (PK-PD) as well as adopting computerized decision support technologies when possible. The United...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/j.diagmicrobio.2018.03.015

    authors： Owens RC Jr,Bulik CC,Andes DR

    更新日期：2018-08-01 00:00:00

  • Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TPN17 and TPN47.

    abstract：:Syphilis remains a worldwide public health problem; it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. Here, we report a new testing method named colloidal gold-immunochromatography assay (GICA) to detect syphilis instead of fluorescent treponemal antibody-absorptio...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.06.019

    authors： Lin LR,Fu ZG,Dan B,Jing GJ,Tong ML,Chen DT,Yu Y,Zhang CG,Yang TC,Zhang ZY

    更新日期：2010-11-01 00:00:00

  • Analytical and clinical validation of novel real-time reverse transcriptase-polymerase chain reaction assays for the clinical detection of swine-origin H1N1 influenza viruses.

    abstract：:During the early stages of the 2009/2010 swine-origin H1N1 influenza A (S-OIV H1N1 FluA) outbreak, the development and validation of sensitive and specific detection methods were a priority for rapid and accurate diagnosis. Between May and June 2009, 2 real-time reverse transcriptase-polymerase chain reaction (rRT-PCR...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.09.020

    authors： Duncan C,Guthrie JL,Tijet N,Elgngihy N,Turenne C,Seah C,Lau R,McTaggart L,Mallo G,Perusini S,Rebbapragada A,Melano R,Low DE,Farrell D,Guyard C

    更新日期：2011-02-01 00:00:00

  • Epidemiological study of non-polio enterovirus neurological infections in children in the region of Monastir, Tunisia.

    abstract：:The epidemiological, virological, and clinical syndromes of 86 children younger than 13 years suspected of having neurological diseases and admitted to Fattouma Bourguiba Hospital of Monastir from January 2002 to November 2003 were analyzed. The presence of enterovirus was studied in cerebrospinal fluid (CSF) samples ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.08.017

    authors： Gharbi J,Jaïdane H,Ben M'hadheb M,El Hiar R,Chouchene C,Gueddiche MN,Aouni M

    更新日期：2006-01-01 00:00:00

  • Development of a diagnostic real-time polymerase chain reaction assay for the detection of invasive Haemophilus influenzae in clinical samples.

    abstract：:Since the introduction of the Haemophilus influenzae serotype b vaccine, invasive H. influenzae disease has become dominated by nontypeable (NT) strains. Several widely used molecular diagnostic methods have been shown to lack sensitivity or specificity in the detection of some of these strains. Novel real-time assays...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.08.018

    authors： Meyler KL,Meehan M,Bennett D,Cunney R,Cafferkey M

    更新日期：2012-12-01 00:00:00

  • Prosthetic valve endocarditis caused by Bartonella quintana in a patient during immunosuppressive therapies for collagen vascular diseases.

    abstract：:Bartonella quintana, known to cause various clinical symptoms, is increasingly recognized as one important cause of culture-negative endocarditis. We report a case of infectious endocarditis with B. quintana on the prosthetic valve, accompanied by proteinase 3-antineutrophil cytoplasmic antibody-positive collagen vasc...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.03.011

    authors： Yamada Y,Ohkusu K,Yanagihara M,Tsuneoka H,Ezaki T,Tsuboi J,Okabayashi H,Suwabe A

    更新日期：2011-07-01 00:00:00

  • Clinical, immunological and microbiological predictors of poor outcome in Clostridium difficile infection.

    abstract：BACKGROUND:Clostridium difficile infection (CDI) causes increased morbidity and mortality. Clinical data cannot clearly predict poor CDI outcome. Data on the value of microbiological predictors is scarce. OBJECTIVE:To identify early predictors of poor outcome of CDI. METHODS:We prospectively included patients with CD...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.05.005

    authors： Reigadas E,Alcalá L,Marín M,Martín A,Bouza E

    更新日期：2017-08-01 00:00:00

  • Antibody test for Legionella pneumophila detection.

    abstract：:Legionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested again...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.11.005

    authors： Párraga-Niño N,Quero S,Uria N,Castillo-Fernandez O,Jimenez-Ezenarro J,Muñoz FX,Sabrià M,Garcia-Nuñez M

    更新日期：2018-02-01 00:00:00

  • The first case of endophthalmitis due to Rhinocladiella basitona in an immunocompetent patient.

    abstract：:Rhinocladiella, a genus of black yeast-like fungi, is related to many infections in humans, including not only mild cutaneous lesions but also fatal brain infections. However, endophthalmitis caused by Rhinocladiella has never been reported by far. Herein, we present the first case of endophthalmitis due to Rhinocladi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.05.014

    authors： Liu M,Xin X,Li J,Chen S

    更新日期：2015-09-01 00:00:00

  • Comparison of a conventional and nested PCR for diagnostic confirmation and genotyping of Orientia tsutsugamushi.

    abstract：:A nested polymerase chain reaction (PCR) targeting the 56-kDa antigen gene is currently the most commonly used molecular technique for confirmation of scrub typhus and genotyping of Orientia tsutsugamushi. In this study, we have compared the commonly used nested PCR (N-PCR) with a single-step conventional PCR (C-PCR) ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.11.030

    authors： Janardhanan J,Prakash JA,Abraham OC,Varghese GM

    更新日期：2014-05-01 00:00:00

  • Anti-Mycoplasma pneumoniae secretory antibody in human breast milk.

    abstract：:The secretory antibody responses to M. pneumoniae polypeptides were assessed among human breast milk samples. M. pneumoniae polypeptides were resolved with conventional SDS-PAGE, and antibody responses were determined by immunoblotting. Antibodies that contained secretory component were found to recognize antigens of ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(02)00396-6

    authors： Macadam S,Cimolai N

    更新日期：2002-07-01 00:00:00

  • Kinetics of CMV seroconversion in a Swiss pregnant women population.

    abstract：:Retrospective evaluation of the kinetics of cytomegalovirus (CMV) seroconversion with CMV IgM, IgG, and IgG avidity assays, in a Swiss pregnant women population, has shown that the current published CMV serologic diagnostic algorithms were valid and fit for use. In 19% of the cases analyzed, CMV-specific IgM was detec...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.03.016

    authors： Maine GT,Stricker R,Stricker R

    更新日期：2012-07-01 00:00:00

  • Carbapenem therapy for bacteremia due to extended-spectrum β-lactamase-producing Escherichia coli or Klebsiella pneumoniae.

    abstract：:For 244 patients with bacteremia due to extended-spectrum β-lactamase (ESBL)-producing Escherichia coli or Klebsiella pneumoniae treated by ertapenem (73, 29.9%) or either imipenem or meropenem (171, 70.1%), the therapeutic efficacy was evaluated. Ertapenem therapy was effective for patients with ESBL-producing E. col...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.12.008

    authors： Lee NY,Huang WH,Tsui KC,Hsueh PR,Ko WC

    更新日期：2011-05-01 00:00:00

  • Drug-induced lymphocyte stimulation test in the prediction of drug-induced hypersensitivity to antituberculosis drugs.

    abstract：:Antituberculosis (TB) chemotherapeutic drugs may cause a variety of adverse drug reactions (ADRs). To assess the potential of drug-induced lymphocyte stimulation test (DLST) in screening ADRs in patients treated with anti-TB drugs, we performed DLST in 272 TB patients (176 cases with ADRs and 96 controls without ADRs)...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.03.008

    authors： Sun Q,Sha W,Gui XW,Xiao YJ,Zeng WH,Sun WW,Xiao HP,Ye WY

    更新日期：2015-06-01 00:00:00

  • Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY Antimicrobial Surveillance Program.

    abstract：:To validate the current National Committee for Clinical Laboratory Standards recommendations of the cefoxitin disk as a preferred surrogate marker to detect oxacillin resistance in staphylococcal isolates, 304 staphylococcal isolates originating from 49 sites in 16 countries in the SENTRY Antimicrobial Surveillance Pr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.08.002

    authors： Pottumarthy S,Fritsche TR,Jones RN

    更新日期：2005-01-01 00:00:00

  • Low prevalence of Pneumocystis jirovecii lung colonization in Ugandan HIV-infected patients hospitalized with non-Pneumocystis pneumonia.

    abstract：:Pneumocystis jirovecii is an important opportunistic infection in human immunodeficiency virus (HIV)-infected patients. In the developed world, P. jirovecii epidemiology is marked by frequent colonization in immunosuppressed patients, but data on the prevalence of colonization are very limited in sub-Saharan Africa, w...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.10.009

    authors： Taylor SM,Meshnick SR,Worodria W,Andama A,Davis JL,Cattamanchi A,den Boon S,Yoo SD,Goodman CD,Huang L,International HIV-associated Opportunistic Pneumonias (IHOP) Study.

    更新日期：2012-02-01 00:00:00

  • Effectual detection of group B streptococci with reduced penicillin susceptibility (PRGBS) by commercially available methicillin-resistant-Staphylococcus aureus (MRSA)-selective agar.

    abstract：:We evaluated the feasibility and efficacy of a commercially available methicillin-resistant Staphylococcus aureus (MRSA)-selective agar, chromID(™) MRSA, to detect group B streptococci with reduced penicillin susceptibility (PRGBS) in this study. The results showed 72.4% (21/29) sensitivity and 98.4% (60/61) specifici...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.04.018

    authors： Fukigai S,Morimoto M,Kimura K,Doyama Y,Miyazaki A,Kamiya C,Banno H,Morishima E,Onoda T,Nagano N,Jin W,Wachino JI,Yamada K,Arakawa Y

    更新日期：2016-07-01 00:00:00

  • Prevalence of beta-lactam nonsusceptible Gram-negative bacilli and use and interpretation of current susceptibility breakpoints: a survey of infectious disease physicians.

    abstract：:Beta-lactam-resistant Enterobacteriaceae represent an important public health problem; however, questions exist about their prevalence and the impact of recent breakpoint changes on clinical practice. We surveyed infectious disease physicians to better understand these issues. Many reported encountering resistant Ente...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.07.013

    authors： Kallen AJ,Beekmann SE,Limbago B,Lentnek AL,Polgreen PM,Patel J,Srinivasan A

    更新日期：2011-11-01 00:00:00

  • Change in the clinical antifungal sensitivity profile of Aspergillus flavus induced by azole and a benzimidazole fungicide exposure.

    abstract：:The study evaluated the change in the clinical antifungal sensitivity profile of A. flavus strains after exposure to azole and benzimidazole fungicide. Exposure to fungicide altered the sensitivity profile for the antifungal itraconazole, voriconazole and posaconazole. This change was characterized by an increase in t...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.05.019

    authors： Meireles LM,de Araujo ML,Endringer DC,Fronza M,Scherer R

    更新日期：2019-10-01 00:00:00

  • Antimicrobial resistance among uropathogens that cause acute uncomplicated cystitis in women in Hong Kong: a prospective multicenter study in 2006 to 2008.

    abstract：:A prospective multicenter study was conducted to assess the epidemiology of antimicrobial resistance among uropathogens causing uncomplicated cystitis. Adult women with clinical diagnosis of uncomplicated cystitis were enrolled from 54 participating centers distributed all over Hong Kong during 2006 to 2008. A positiv...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.diagmicrobio.2009.03.027

    authors： Ho PL,Yip KS,Chow KH,Lo JY,Que TL,Yuen KY

    更新日期：2010-01-01 00:00:00

  • Phaeomycotic cysts caused by Phoma species.

    abstract：:Phoma species are primarily phytopathogens which have been reported to sporadically cause human disease. We report a patient with phaeohyphomycotic cysts caused by Phoma species, which were initially mistaken for ganglions. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.04.009

    authors： Vasoo S,Yong LK,Sultania-Dudani P,Scorza ML,Sekosan M,Beavis KG,Huhn GD

    更新日期：2011-08-01 00:00:00