Abstract:
:A nested polymerase chain reaction (PCR) targeting the 56-kDa antigen gene is currently the most commonly used molecular technique for confirmation of scrub typhus and genotyping of Orientia tsutsugamushi. In this study, we have compared the commonly used nested PCR (N-PCR) with a single-step conventional PCR (C-PCR) for amplification and genotyping. Eschar samples collected from 24 patients with scrub typhus confirmed by IgM enzyme-linked immunosorbent assay were used for DNA extraction following which amplifications were carried out using nested and C-PCR methods. The amplicons were sequenced and compared to other sequences in the database using BLAST. Conventional PCR showed a high positivity rate of 95.8% compared to the 75% observed using N-PCR. On sequence analysis, the N-PCR amplified region showed more variation among strains than the C-PCR amplified region. The C-PCR, which is more economical, provided faster and better results compared to N-PCR.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Janardhanan J,Prakash JA,Abraham OC,Varghese GMdoi
10.1016/j.diagmicrobio.2013.11.030subject
Has Abstractpub_date
2014-05-01 00:00:00pages
7-9issue
1eissn
0732-8893issn
1879-0070pii
S0732-8893(14)00027-3journal_volume
79pub_type
杂志文章abstract::The in vitro activities of HMR 3647, erythromycin A, clarithromycin, azithromycin, roxithromycin, penicillin G, ampicillin, cefuroxime, trimethoprim/sulfamethoxazole, tetracycline, ciprofloxacin, and levofloxacin were determined for 1179 Streptococcus pneumoniae, 1438 Haemophilus influenzae, and 428 Moraxella catarrha...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,多中心研究
doi:10.1016/s0732-8893(99)00053-x
更新日期:1999-09-01 00:00:00
abstract::The diagnosis of tuberculosis (TB) is difficult in children. The pediatricians are waiting for new and rapid tests that are easy to realize and that are performed better than tuberculin skin test (TST). We presented here the evaluation of QuantiFERON-TB Gold In-Tube (QFT-G IT) in the Children Hospital of Nancy. Fifty-...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2009.11.002
更新日期:2010-04-01 00:00:00
abstract::ST252 Enterobacter cloacae, producing GES-5 carbapenemase, was isolated in a Czech hospital. blaGES-5 was part of a novel class 1 integron, In1406, which also included a new allele of the aadA15 gene cassette. In1406 was located on a ColE2-like plasmid, pEcl-35771cz (6953bp). ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2017.10.011
更新日期:2018-02-01 00:00:00
abstract::We evaluated all Candida sp. bloodstream isolates obtained from patients admitted to 4 tertiary care hospitals between 1995 and 2003 in the city of São Paulo, Brazil. Susceptibility to amphotericin B, 5-fluorocytosine, fluconazole (FCZ), itraconazole (ITZ), and voriconazole (VCZ) was determined using the Clinical Labo...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章,多中心研究
doi:10.1016/j.diagmicrobio.2006.10.011
更新日期:2007-04-01 00:00:00
abstract::We present a case report of Mycobacterium malmoense in a 53-year-old white man. The incidence of M. malmoense infections is a rare event compared with other nontuberculous mycobacteria, but it has increased since 1980, especially in northern Europe. Many patients have disposing underlying diseases. In most cases, it i...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2009.07.004
更新日期:2010-01-01 00:00:00
abstract::Salmonella enterica serovar Enteritidis (S. Enteritidis) is the most common causative agent of non-typhoidal salmonellosis in Malaysia. We aimed to characterize S. Enteritidis isolated from humans and animals by analyzing their antimicrobial resistance profiles and genotypes. A total of 111 strains were characterized ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2013.09.004
更新日期:2013-12-01 00:00:00
abstract::A commercially available (Electro-Nucleonics, Inc.) indirect fluorescent antibody test was evaluated for the detection of cytomegalovirus-specific IgM antibody in serum of newborn infants. The serum IgM fraction from 13 symptomatic newborns with cytomegalovirus infection and 30 control infants was tested. Seven of 13 ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(87)90007-1
更新日期:1987-07-01 00:00:00
abstract::Ten human sera were used to study different parameters, namely, methods of smear preparation and fixation, and age of infected HSB-2 cells in order to optimize indirect immunofluorescence assay (IFA) and anticomplement immunofluorescence (ACIF) procedures to measure antibody levels against HHV-6. Results showed a grea...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(92)90017-n
更新日期:1992-05-01 00:00:00
abstract::Antibacterial activity of sulbactam or ampicillin alone and in combination on ampicillin-resistant, beta-lactamase-producing Gram-negative bacteria (Citrobacter freundii and Escherichia coli) was studied. Inhibition of beta-lactamase activity by sulbactam was investigated using intact and disrupted cells. Minimal inhi...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(88)90079-x
更新日期:1988-10-01 00:00:00
abstract::We report a comparative analysis of intravascular catheter-related infection before and after routine use of antibiotic-bonded catheters in an intensive care unit. Cefazolin-bonded catheters were placed in patients requiring catheterization for at least 3 days, or with remote infection, standard catheters at other tim...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(97)00215-0
更新日期:1998-03-01 00:00:00
abstract::The Gen-probe group A Streptococcus direct test (GASD), a nucleic acid probe assay for detecting GAS from throat swabs, has recently been developed. The test uses an acridium ester-labeled DNA probe which is complementary to the rRNA of Streptococcus pyogenes. In this study, 318 single culturette throat swabs were tes...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(95)00275-8
更新日期:1996-02-01 00:00:00
abstract::The improved Streptex method for serogrouping streptococci incorporates a new extraction enzyme and a simplified procedure requiring no centrifugation. A total of 114 clinical isolates of beta-hemolytic streptococci were serogrouped from primary plates, isolation plates, and Todd-Hewitt broth cultures using this syste...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(84)90006-3
更新日期:1984-04-01 00:00:00
abstract::The ability of nine clinical isolates of Candida species (three C. albicans, three C. tropicalis and three C. parapsilosis) to colonize and invade the gastrointestinal (GI) tract of adult male CD-1 (ICR) mice was determined. The effect of dietary tetracycline plus glucose supplementation on colonization was evaluated....
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(00)00165-6
更新日期:2000-09-01 00:00:00
abstract::The study evaluated the change in the clinical antifungal sensitivity profile of A. flavus strains after exposure to azole and benzimidazole fungicide. Exposure to fungicide altered the sensitivity profile for the antifungal itraconazole, voriconazole and posaconazole. This change was characterized by an increase in t...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2019.05.019
更新日期:2019-10-01 00:00:00
abstract::Predicted and observed failures at higher cefepime MICs have prompted the Clinical and Laboratories Standards Institute (CLSI) to lower the susceptible breakpoint for Enterobacteriaceae to ≤2mg/L, with dose-dependent susceptibility at 4-8mg/L, while the susceptibility breakpoint for nonfermentative organisms remain un...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2015.03.005
更新日期:2015-06-01 00:00:00
abstract::We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (less than 1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mo...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(92)90035-r
更新日期:1992-02-01 00:00:00
abstract::Fusarium is an opportunistic fungal pathogen which is emerging as a significant cause of morbidity and mortality in immunocompromised hosts. We present a rare case of F. verticillioides fungemia that occurred in a patient who underwent a second orthotopic liver transplantation for chronic rejection and completely resp...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2011.08.024
更新日期:2011-12-01 00:00:00
abstract::A total of 28 strains of Haemophilus influenzae (Hi) a and b isolated from clinical samples before and after the introduction of the Hib conjugate vaccine in Brazil were analyzed to determine variants of the capsular gene. Our results suggest the occurrence of new variants closely related to types I and II previously ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2010.05.011
更新日期:2010-10-01 00:00:00
abstract::As new tests and technologies advance our understanding and diagnostic capabilities of the severe acute respiratory syndrome coronavirus 2 and the coronavirus disease 2019, they must be appropriately validated to make sure test performance is following manufacturer claims. In this study, we evaluated the Vazyme 2019-n...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2020.115248
更新日期:2021-02-01 00:00:00
abstract:BACKGROUND:We evaluated the clinical significance of urine cultures from patients with an indwelling urinary catheter (UC) from which 2 different pathogens were isolated. METHODS:Urine cultures from patients with a UC from which 2 different organisms were isolated were randomly divided into a control group (culture re...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2013.01.015
更新日期:2013-05-01 00:00:00
abstract::Clostridium difficile (CD) diagnosis is very varied and under discussion. Different research groups disagree on the clinical significance of patients with negative direct toxin and positive polymerase chain reaction (PCR) or even more with direct toxin and glutamate dehydrogenase (GDH) both negatives, but CD detected ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2020.115050
更新日期:2020-07-01 00:00:00
abstract::We isolated an Escherichia coli O157:H7 strain that was negative for verocytotoxin production, but positive for the vtx2 gene using commercial kits, from an asymptomatic food handler. The laboratory investigations revealed that a 1310-bp insertion sequence, IS1203 variant, was present in the B subunit-coding region of...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2013.06.012
更新日期:2013-10-01 00:00:00
abstract::Ceftobiprole medocaril, the prodrug of ceftobiprole, is an advanced-generation cephalosporin that is approved in many European and non-European countries for the treatment of adults with hospital-acquired pneumonia (excluding ventilator-associated pneumonia) and community-acquired pneumonia and is currently being eval...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2019.01.015
更新日期:2019-07-01 00:00:00
abstract::This retrospective study aimed to evaluate the clinical, laboratory, and quantitative cerebrospinal fluid (CSF) cryptococcal cell counts for associations with in-hospital outcomes of HIV-infected patients with cryptococcal meningitis. Ninety-eight HIV-infected adult patients with CSF culture-proven cryptococcal mening...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2012.01.014
更新日期:2012-05-01 00:00:00
abstract::The in vitro susceptibility of 19 antipseudomonal antibiotics, old and new, were tested against 100 blood isolates of Pseudomonas aeruginosa. The isolates were taken from consecutive bacteremic patients hospitalized in nine Pittsburgh hospitals from 1983 to 1984. Twelve percent of isolates tested were tobramycin-resis...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(87)90027-7
更新日期:1987-06-01 00:00:00
abstract::Gepotidacin (GSK2140944) is a first in class, novel triazaacenaphthylene bacterial type II topoisomerase inhibitor in Phase 3 clinical development for the treatment of gonorrhea and uncomplicated urinary tract infections (acute cystitis). This study tested the equivalency of minimal inhibitory concentrations (MICs) ob...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2020.115107
更新日期:2020-10-01 00:00:00
abstract::Detection of specific IgM antibodies by ELISA forms the mainstay for diagnosis of dengue infection. However, IgM antibodies develop after 4 to 5 days of infection. The methods for early diagnosis include virus isolation and reverse transcriptase polymerase chain reaction (RT-PCR) which need a sophisticated laboratory....
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2010.04.004
更新日期:2010-09-01 00:00:00
abstract::The modified Visuwell Strep-A enzyme immunoassay (EIA) was compared with culture for detection of group-A Streptococcus from throat swabs. Throat swabs in modified Stuarts medium obtained after culture at two institutions were tested in Visuwell. Cumulative results were n = 417, sensitivity 87.8%, specificity 89.9% pr...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/0732-8893(91)90017-a
更新日期:1991-07-01 00:00:00
abstract::The in vitro activity of DU-6859a (DU) alone and in combination with various antimicrobials was evaluated against multiresistant enterococci including some isolates with defined gyrA mutations. DU produced rapid in vitro killing against most enterococci that lacked resistance to ciprofloxacin, but it was not bacterici...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(96)00197-6
更新日期:1996-10-01 00:00:00
abstract::Syphilis remains a worldwide public health problem; it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. Here, we report a new testing method named colloidal gold-immunochromatography assay (GICA) to detect syphilis instead of fluorescent treponemal antibody-absorptio...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2010.06.019
更新日期:2010-11-01 00:00:00