当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TPN17 and TPN47.

Development of a colloidal gold-immunochromatography assay to detect immunoglobulin G antibodies to Treponema pallidum with TPN17 and TPN47.

Abstract：

:Syphilis remains a worldwide public health problem; it is necessary to develop a new diagnostic approach that is easier and faster than conventional tests. Here, we report a new testing method named colloidal gold-immunochromatography assay (GICA) to detect syphilis instead of fluorescent treponemal antibody-absorption (FTA-Abs). Syphilis-specific immunoglobulin G (IgG) antibody was detected with GICA established on syphilis-specific recombinant proteins, TPN17 and TPN47. FTA-Abs Treponema pallidum (TP)-IgG was set as the gold standard. A GICA test was performed to detect the serum of 14 967 subjects who took a serologic test for syphilis at the Xiamen Center of Clinical Laboratory, Fujian, China, from March 2009 to February 2010, among which 1326 cases were diagnosed as syphilitic. The results showed that the sensitivity, specificity, and positive predictive value were 99.38% (1279/1287), 99.96% (12,975/12,980), and 99.61% (1279/1284), respectively. The positive rate between the 2 test methods had no significant difference (χ(2) = 0.003, P > 0.05). Detection on 500 interference specimens indicated that the biologic false-positive rate of the GICA test was extremely low and free from other biologic and chemical factors. The characteristics of GICA TP-IgG correspond to that of FTA-Abs TP-IgG (EUROIMMUN Medizinische Labordiagnostika, Germany). The GICA test is convenient, fast, and inexpensive, and it can be used both as a confirmatory test and a screening indicator, instead of FTA-Abs TP-IgG.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Lin LR,Fu ZG,Dan B,Jing GJ,Tong ML,Chen DT,Yu Y,Zhang CG,Yang TC,Zhang ZY

doi

10.1016/j.diagmicrobio.2010.06.019

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

193-200

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(10)00251-8

journal_volume

68

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • Effect of 10 minutes heat treatment on HIV antibody testing from alternate testing sites.

    abstract：:One hundred and thirty-five sera were tested for the presence of antibody to human immunodeficiency virus (HIV) by enzyme immunoassay (EIA) with the Abbott Diagnostics HTLV-III test before and after heating at 56 degrees C for 10 min. Only one serum specimen was repeatedly reactive after heating when compared with the...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(88)90113-7

    authors： Markowski MA,Coard JG,Griffith B,Mayo DR

    更新日期：1988-04-01 00:00:00

  • Combination of staphylococcal chromosome cassette SCCmec type V and Panton-Valentine leukocidin genes in a methicillin-resistant Staphylococcus aureus that caused necrotizing pneumonia in Greece.

    abstract：:We describe a case of necrotizing pneumonia in Greece caused by a community-acquired methicillin-resistant Staphylococcus aureus (MRSA) producing Panton-Valentine leukocidin (PVL) carrying the staphylococcal chromosome cassette mec (SCCmec) type V. The pulse field gel electrophoresis (PFGE) pattern of this strain diff...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.06.005

    authors： Gerogianni I,Mpatavanis G,Gourgoulianis K,Maniatis A,Spiliopoulou I,Petinaki E

    更新日期：2006-10-01 00:00:00

  • Prevalence and epidemiology of microbial pathogens causing bloodstream infections: results of the OASIS multicenter study.

    abstract：:Beginning on April 2007, a prospective multicenter study was performed to investigate prevalence and epidemiology of microbial pathogens causing bloodstream infections (BSIs). Twenty microbiology laboratories participated to the survey over a 1-year period. A total of 11,638 episodes of BSI occurred in 11 202 patients...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.diagmicrobio.2010.10.016

    authors： Luzzaro F,Ortisi G,Larosa M,Drago M,Brigante G,Gesu G

    更新日期：2011-04-01 00:00:00

  • Evaluation of commercial serologic test reagents for immunoidentification of medically important aspergilli.

    abstract：:We evaluated commercial serodiagnostic test reagents from Greer Laboratories (GL), Lenoir, NC; Immuno-mycologics, Inc. (IMI), Norman, OK; and Scott Laboratories (SL), Fiskville, RI; for their ability to detect Aspergillus spp. exoantigens and group them in their proper series. We detected 87 culture extracts from code...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90170-2

    authors： Sekhon AS,Standard PG,Kaufman L,Garg AK

    更新日期：1987-11-01 00:00:00

  • Susceptibility testing of Mycobacterium tuberculosis by broth microdilution method: a rapid alternative method.

    abstract：:A total of 50 clinical isolates of Mycobacterium tuberculosis were tested by the proportion method and the broth microdilution method (BMM) for primary antitubercular drugs. The results were obtained after 14 days of incubation. There was a 100% agreement for all drugs except streptomycin, which showed a 96% agreement...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.10.001

    authors： Sethi S,Mandal J,Kumar P,Kumar S,Meharwal S,Jindal SK,Sharma M

    更新日期：2007-04-01 00:00:00

  • Hemophilus aphrophilus vertebral osteomyelitis: a case report and literature review.

    abstract：:We report a 60-yr-old diabetic female who developed vertebral osteomyelitis and discitis due to Hemophilus aphrophilus, and review three previously reported cases of this uncommon infection. Predisposing factors, clinical features, and treatment of H. aphrophilus vertebral osteomyelitis are discussed. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/0732-8893(87)90171-4

    authors： Gribble MJ,Hunter T

    更新日期：1987-11-01 00:00:00

  • Varicella associated intracerebral hemorrhage in the absence of thrombocytopenia.

    abstract：:A young woman with varicella complicated by streptococcal cellulitis developed an intracerebral bleed in the absence of thrombocytopenia, serious coagulopathy, or a detectable vascular lesion. We postulate that during the acute infection with varicella, a cerebral endothelial lesion was produced which later caused the...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(89)90103-x

    authors： Fikrig E,Barg NL

    更新日期：1989-07-01 00:00:00

  • Reproducibility of gemifloxacin and comparison fluoroquinolone MIC results using Sensititre commercial dry-form panels.

    abstract：:This study examined the ability of commercial dry-form broth microdilution panels (Sensititre, TREK Diagnostics, Cleveland, OH) to produce accurate minimum inhibitory concentration (MIC) results for gemifloxacin, a new fluoroquinolone. Reproducibility for gemifloxacin was 100.0% for on-scale values within +/-1 log2 di...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.11.005

    authors： Ross JE,Sader HS,Fritsche TR,Jones RN

    更新日期：2005-03-01 00:00:00

  • Emergence of metallo-β-lactamases in Enterobacteriaceae from Argentina.

    abstract：:Carbapenem susceptibility in Enterobacteriaceae (M9921, M9959) revealed the presence of MBLs bla(VIM-2) (M9959) and bla(IMP-8) (M9921), both as first cassettes of class-1-integrons. ESBL bla(PER-2) was detected in both strains and M9921 also harboured qnrB10, aac(6')-Ib and aac(6')-Ib-cr. This is the first report of M...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.08.025

    authors： Gomez S,Rapoport M,Togneri A,Viegas-Caetano J,Faccone D,Corso A,Petroni A,Pasteran F

    更新日期：2011-01-01 00:00:00

  • Pharmacodynamic comparisons of antimicrobials against nosocomial isolates of escherichia coli, klebsiella pneumoniae, acinetobacter baumannii and pseudomonas aeruginosa from the MYSTIC surveillance program: the OPTAMA Program, South America 2002.

    abstract：:The OPTAMA (Optimizing Pharmacodynamic Target Attainment using the MYSTIC [Meropenem Yearly Susceptibility Test Information Collection] Antibiogram) Program provides insight into the appropriate antibiotic options for empiric therapy for common nosocomial pathogens. In this report, South America is represented by Braz...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.03.003

    authors： Kiffer CR,Mendes C,Kuti JL,Nicolau DP

    更新日期：2004-06-01 00:00:00

  • Direct identification and antimicrobial susceptibility testing of microorganisms from positive blood cultures following isolation by lysis-centrifugation.

    abstract：:We evaluated a direct from positive blood culture pelleting procedure that utilizes a lysis-centrifugation protocol for the identification of microorganisms by MALDI-TOF MS with subsequent antimicrobial susceptibility testing (AST) and rapid methicillin- and beta-lactam-resistance screening. The identification evaluat...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2018.06.010

    authors： Campigotto A,Goneau L,Matukas LM

    更新日期：2018-11-01 00:00:00

  • Performance characteristics of the high sensitivity Alinity i & ARCHITECT HBsAg Next Qualitative/Confirmatory assays.

    abstract：:Despite improvement in vaccinations, Hepatitis B remains a major health concern due to the difficulty of prevention even in low endemic areas such as Europe. In this report we describe the performance characteristics of the new HBsAg Next Qualitative and HBsAg Next Confirmatory assays designed for blood screening and ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115033

    authors： Sickinger E,Braun HB,Meyer T,Schmid K,Daghfal D,Oer M,Schultess J

    更新日期：2020-06-01 00:00:00

  • In vitro activity of l-ofloxacin against norfloxacin-resistant coagulase-negative staphylococci.

    abstract：:The in vitro activity of l-ofloxacin was determined against coagulase-negative staphylococci that were induced to norfloxacin resistance. l-Ofloxacin was the most active agent tested with an MIC90 of 4 micrograms/ml compared with greater than 128, 32, and 128 micrograms/ml for norfloxacin, ciprofloxacin, and enoxacin,...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90109-7

    authors： Foleno B,Fu KP

    更新日期：1992-08-01 00:00:00

  • Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry.

    abstract：:The application of matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry (MS) directly to blood culture broth has potential to identify bloodstream infection earlier and facilitate timely management. We prospectively tested a novel, rapid, and inexpensive in-house spin-lysis protocol...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.06.016

    authors： Gray TJ,Thomas L,Olma T,Iredell JR,Chen SC

    更新日期：2013-10-01 00:00:00

  • Drug-induced lymphocyte stimulation test in the prediction of drug-induced hypersensitivity to antituberculosis drugs.

    abstract：:Antituberculosis (TB) chemotherapeutic drugs may cause a variety of adverse drug reactions (ADRs). To assess the potential of drug-induced lymphocyte stimulation test (DLST) in screening ADRs in patients treated with anti-TB drugs, we performed DLST in 272 TB patients (176 cases with ADRs and 96 controls without ADRs)...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.03.008

    authors： Sun Q,Sha W,Gui XW,Xiao YJ,Zeng WH,Sun WW,Xiao HP,Ye WY

    更新日期：2015-06-01 00:00:00

  • Mycetoma of the scalp due to Microsporum canis: hystopathologic, mycologic, and immunogenetic features in a 6-year-old girl.

    abstract：:Dermatophytic mycetoma is an extremely rare subcutaneous mycosis. Here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by Microsporum canis. To our knowledge, this is the first report showing the immunologic and immunogenetic fea...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.02.003

    authors： Chiapello LS,Dib MD,Nuncira CT,Nardelli L,Vullo C,Collino C,Abiega C,Cortes PR,Spesso MF,Masih DT

    更新日期：2011-05-01 00:00:00

  • Effectual detection of group B streptococci with reduced penicillin susceptibility (PRGBS) by commercially available methicillin-resistant-Staphylococcus aureus (MRSA)-selective agar.

    abstract：:We evaluated the feasibility and efficacy of a commercially available methicillin-resistant Staphylococcus aureus (MRSA)-selective agar, chromID(™) MRSA, to detect group B streptococci with reduced penicillin susceptibility (PRGBS) in this study. The results showed 72.4% (21/29) sensitivity and 98.4% (60/61) specifici...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.04.018

    authors： Fukigai S,Morimoto M,Kimura K,Doyama Y,Miyazaki A,Kamiya C,Banno H,Morishima E,Onoda T,Nagano N,Jin W,Wachino JI,Yamada K,Arakawa Y

    更新日期：2016-07-01 00:00:00

  • Pharmacokinetics of polymyxin B1 in patients with multidrug-resistant Gram-negative bacterial infections.

    abstract：:Polymyxin B is increasingly used clinically for the treatment of multidrug-resistant Gram-negative infections, despite very limited understanding of its disposition in humans. The disposition of intravenous polymyxin B1 in 9 adult patients was characterized. Random blood samples (specifically timed in relation to the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2007.08.008

    authors： Kwa AL,Lim TP,Low JG,Hou J,Kurup A,Prince RA,Tam VH

    更新日期：2008-02-01 00:00:00

  • Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples.

    abstract：:We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2016.01.013

    authors： Huang W,Wang G,Lin H,Zhuge J,Nolan SM,Vail E,Dimitrova N,Fallon JT

    更新日期：2016-05-01 00:00:00

  • Major methodology-dependent discordant susceptibility results for Bacteroides fragilis group isolates but not other anaerobes.

    abstract：:Two standardized susceptibility test methods, a broth microdilution (BMD) and agar dilution (AD) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. Against the 339 strains of the Bacteroides fragilis group BMD mi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(94)90107-4

    authors： Aldridge KE,Schiro DD

    更新日期：1994-11-01 00:00:00

  • Bacteriological studies with ampicillin/sulbactam on selected strains of gram-negative bacteria.

    abstract：:Antibacterial activity of sulbactam or ampicillin alone and in combination on ampicillin-resistant, beta-lactamase-producing Gram-negative bacteria (Citrobacter freundii and Escherichia coli) was studied. Inhibition of beta-lactamase activity by sulbactam was investigated using intact and disrupted cells. Minimal inhi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(88)90079-x

    authors： Bello H,del Solar E,González C,Garcia A,Urrea R,Zemelman R

    更新日期：1988-10-01 00:00:00

  • Screening the enteroaggregative Escherichia coli activity and detection of the aggA, aafA, and astA genes with novel PCR primers for the Escherichia coli isolates from diarrhea cases in Taiwan.

    abstract：:Enteroaggregative Escherichia coli (EAggEC) are emerging enteropathogens associated with human diarrhea diseases and food poisoning cases. They show distinctive aggregative pattern of adherence to cultured human epithelial cells. However, EAggEC strains are diverse and not all of them have the aggregative adherence fi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00043-9

    authors： Tsai CC,Chen SY,Tsen HY

    更新日期：2003-07-01 00:00:00

  • Bartonella quintana-induced apoptosis inhibition of human endothelial cells is associated with p38 and SAPK/JNK modulation and with stimulation of mitosis.

    abstract：:Previous studies demonstrated that live Bartonella quintana often induces angioproliferative lesions in humans. It modulates endothelial cell apoptotic and inflammatory patterns, thus inducing a very early overexpression of caspase 8 and Apaf-1 and increasing mRNA production of TNF-alpha, interleukin-8, and E-selectin...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.06.016

    authors： Liberto MC,Matera G,Lamberti AG,Barreca GS,Focà D,Quirino A,Soria MR,Focà A

    更新日期：2004-11-01 00:00:00

  • Diagnostic value of an enzyme-linked immunospot assay for interferon-γ in genitourinary tuberculosis.

    abstract：:The aim of this study was to evaluate the diagnostic performance of an enzyme-linked immunospot (ELISPOT) assay for interferon-γ in patients with suspected genitourinary tuberculosis (TB). A total of 30 patients with suspected genitourinary TB at the National Taiwan University Hospital, Taipei, Taiwan, were prospectiv...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.07.006

    authors： Lai CC,Tan CK,Lin SH,Liao CH,Huang YT,Wang CY,Wang JY,Lin HI,Hsueh PR

    更新日期：2010-11-01 00:00:00

  • Prototheca zopfii isolated from a patient with olecranon bursitis.

    abstract：:Prototheca zopfii was isolated from a patient with olecranon bursitis. Olecranon bursitis caused by Prototheca is a distinct clinical entity. Recognition of this infection was made by observing characteristic organisms in tissue and subsequent isolation of the organism. ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90103-9

    authors： Naryshkin S,Frank I,Nachamkin I

    更新日期：1987-02-01 00:00:00

  • Application of a serum-free medium in the growth and differentiation of human peripheral blood lymphocytes.

    abstract：:To eliminate variability due to nonspecific stimulation or inhibition by different lots of fetal bovine serum, the activation of human peripheral blood mononuclear cells in a modification of Iscove's medium (medium C-IMDM) was compared with the routinely used Roswell Park Memorial Institute (RPMI)-1640 medium containi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(86)90099-4

    authors： Lipson SM

    更新日期：1986-03-01 00:00:00

  • The clinical significance of isolation of two different organisms from the urine of patients with an indwelling catheter.

    abstract：BACKGROUND:We evaluated the clinical significance of urine cultures from patients with an indwelling urinary catheter (UC) from which 2 different pathogens were isolated. METHODS:Urine cultures from patients with a UC from which 2 different organisms were isolated were randomly divided into a control group (culture re...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.01.015

    authors： Wiener-Well Y,Gofman I,Assous MV,Freier-Dror Y,Yinnon AM,Lachish T

    更新日期：2013-05-01 00:00:00

  • Predicting β-lactam resistance using whole genome sequencing in Klebsiella pneumoniae: the challenge of β-lactamase inhibitors.

    abstract：:Although multiple antimicrobial resistance (AMR) determinants can confer the same in vitro antimicrobial susceptibility testing (AST) phenotype, their differing effect on optimal therapeutic choices is uncertain. Using a large population-based collection of clinical strains spanning a 3.5-year period, we applied WGS t...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2020.115149

    authors： Hujer AM,Long SW,Olsen RJ,Taracila MA,Rojas LJ,Musser JM,Bonomo RA

    更新日期：2020-11-01 00:00:00

  • Use of nucleic acid probes for the detection of sexually transmitted infectious agents.

    abstract：:Deoxyribonucleic acid sequences specific for a pathogen of interest can be isolated from a variety of microorganisms. Such DNA probes can be exploited to detect infectious agents directly in infected patient material despite the presence of large numbers of other organisms and host DNA. This technology is dependent up...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/s0732-8893(86)80048-7

    authors： Horn JE,Quinn T,Hammer M,Palmer L,Falkow S

    更新日期：1986-03-01 00:00:00

  • Evaluation of VITEK 2, MicroScan, and Phoenix for identification of clinical isolates and reference strains.

    abstract：:To compare the identification accuracies of VITEK 2 (bioMérieux), MicroScan (Siemens Healthcare), and Phoenix (Becton Dickinson), microbial identification was performed on 160 clinical isolates and 50 reference strains on each of these 3 systems, using the appropriate identification kit provided by each system. Of the...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.04.013

    authors： Jin WY,Jang SJ,Lee MJ,Park G,Kim MJ,Kook JK,Kim DM,Moon DS,Park YJ

    更新日期：2011-08-01 00:00:00