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Use of nucleic acid probes for the detection of sexually transmitted infectious agents.

Abstract：

:Deoxyribonucleic acid sequences specific for a pathogen of interest can be isolated from a variety of microorganisms. Such DNA probes can be exploited to detect infectious agents directly in infected patient material despite the presence of large numbers of other organisms and host DNA. This technology is dependent upon the recognition of a specific nucleotide sequence present in DNA extracted from a clinical sample by a radiolabeled or nonisotopically labeled DNA probe. We have isolated several DNA probes for the detection of pathogenic Neisseria which include a plasmid species unique to the gonococcus, as well as several cloned genes that detect both the gonococcus and the meningococcus. In addition, we characterized a unique plasmid of Chlamydia trachomatis that has proved to be quite useful as a DNA probe for the detection of this pathogen in cervical secretions and, by in situ hybridization, in Papanicolaou smears. Deoxyribonucleic acid hybridization as a diagnostic tool is still in its infancy. It holds a number of advantages over conventional methods of pathogen detection and serves as an alternative, as well as a complement, to available immunologic methods.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Horn JE,Quinn T,Hammer M,Palmer L,Falkow S

doi

10.1016/s0732-8893(86)80048-7

subject

Has Abstract

pub_date

1986-03-01 00:00:00

pages

101S-109S

issue

3 Suppl

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(86)80048-7

journal_volume

4

pub_type

杂志文章,评审

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