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Detection of Clostridium difficile toxins A, B and binary toxin with slow off-rate modified aptamers.

Abstract：

:Rapid and accurate diagnostic tests for Clostridium difficile infections (CDI) are crucial for management of patients with suspected CDI and for infection control. Enzyme immunoassays for detection of the toxins are routinely used but lack adequate sensitivity. We generated slow off-rate modified aptamers (SOMAmer™ reagents) via in vitro selection (SELEX) that bind toxins A, B and binary toxin with high affinity and specificity. Using SOMAmers alone or in conjunction with antibodies, we have developed toxin assays with a 1 pmol/L (300 pg/mL) limit of detection and a 3 log dynamic range. SOMAmers proved useful as capture or detection agents in equilibrium solution binding radioassays, pull-down capture assays, dot blots, and plate- or membrane-based sandwich assays, thus represent a promising alternative to antibodies in diagnostic applications. SOMAmers detected toxins A, B and binary toxin in culture supernatants from toxigenic C. difficile, including a BI/NAP1 strain and historic strains.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Ochsner UA,Katilius E,Janjic N

doi

10.1016/j.diagmicrobio.2013.03.029

subject

Has Abstract

pub_date

2013-07-01 00:00:00

pages

278-85

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(13)00201-0

journal_volume

76

pub_type

杂志文章

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