Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay.

Abstract:

:The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ-producing cells using enzyme-linked immunosorbent spot assay. IFN-γ mRNA was evaluated as an indicator of IGRA in comparison with QFT-IT IFN-γ ELISA in 46 subjects with active TB and in 73 at low risk for TB. Significant IFN-γ mRNA expression was detected from 30 min and peaked 4 h after stimulation with MTB antigens or mitogen. This was defined as the optimal time point for IFN-γ mRNA real-time polymerase chain reaction (PCR). The sensitivities of IFN-γ mRNA real-time PCR and IFN-γ ELISA were 84.8% (39/46) and 89.1% (41/46), respectively (no significant difference). Although the specificities of IFN-γ ELISA was 4.1% higher than that of IFN-γ mRNA real-time PCR (60.3% versus 56.2%), the difference was not statistically significant. The overall agreement between IFN-γ mRNA real-time PCR and IFN-γ ELISA was 79.8% (kappa = 0.475). Whilst there was no difference in the performance of IFN-γ mRNA real-time PCR and IFN-γ ELISA, IFN-γ mRNA real-time PCR was superior to IFN-γ ELISA in terms of the time required for detection of MTB infection.

authors

Kim S,Kim YK,Lee H,Cho JE,Kim HY,Uh Y,Kim YM,Kim H,Cho SN,Jeon BY,Lee H

doi

10.1016/j.diagmicrobio.2012.09.015

subject

Has Abstract

pub_date

2013-01-01 00:00:00

pages

68-72

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(12)00393-8

journal_volume

75

pub_type

杂志文章
  • Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus.

    abstract::We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA su...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.06.006

    authors: Mc Gann P,Milillo M,Kwak YI,Quintero R,Waterman PE,Lesho E

    更新日期:2013-11-01 00:00:00

  • In vitro resistance of clinical Fusarium species to amphotericin B and voriconazole using the EUCAST antifungal susceptibility method.

    abstract::Susceptibility testing using the EUCAST-AFST method against 39 clinical Fusarium strains consecutively collected from local and invasive infections during the last 10years assessed the in vitro activities of amphotericin B (AmB) and triazole antifungal agents. In addition, the susceptibility pattern of 12 reference st...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2016.05.006

    authors: Taj-Aldeen SJ,Salah H,Al-Hatmi AM,Hamed M,Theelen B,van Diepeningen AD,Boekhout T,Lass-Flörl C

    更新日期:2016-08-01 00:00:00

  • Corynebacterium macginleyi isolation from conjunctival swab in Italy.

    abstract::Corynebacterium macginleyi was isolated from conjunctival swabs of a farmer suffering from purulent conjunctivitis. This species has only recently been reported in Switzerland and Germany to be exclusively isolated from ocular surfaces. This represents the first isolation of C. macginleyi in Italy indicating that its ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00438-8

    authors: Giammanco GM,Di Marco V,Priolo I,Intrivici A,Grimont F,Grimont PA

    更新日期:2002-10-01 00:00:00

  • Clinical evaluation of a new single-tube multiplex reverse transcription PCR assay for simultaneous detection of 11 respiratory viruses, Mycoplasma pneumoniae and Chlamydia in hospitalized children with acute respiratory infections.

    abstract::Respiratory Pathogen 13 Detection Kit (13× kit) is able to simultaneously detect 11 respiratory viruses, Mycoplasma pneumoniae (MP) and Chlamydia in a single reaction. Using 572 Nasopharyngeal aspirates collected from hospitalized children, the clinical performance of 13× kit for detecting 11 respiratory viruses was e...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.03.010

    authors: Zhao MC,Li GX,Zhang D,Zhou HY,Wang H,Yang S,Wang L,Feng ZS,Ma XJ

    更新日期:2017-06-01 00:00:00

  • Candida lusitaniae septic arthritis: case report and review of the literature.

    abstract::Candida lusitaniae is an infrequently encountered Candida species that has been associated with resistance to amphotericin B. We present a case of septic arthritis with C. lusitaniae and provide a brief review of the organism, especially in regard to current information about its pattern of resistance to antifungal ag...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/j.diagmicrobio.2007.12.003

    authors: Bariola JR,Saccente M

    更新日期:2008-05-01 00:00:00

  • Change in the clinical antifungal sensitivity profile of Aspergillus flavus induced by azole and a benzimidazole fungicide exposure.

    abstract::The study evaluated the change in the clinical antifungal sensitivity profile of A. flavus strains after exposure to azole and benzimidazole fungicide. Exposure to fungicide altered the sensitivity profile for the antifungal itraconazole, voriconazole and posaconazole. This change was characterized by an increase in t...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2019.05.019

    authors: Meireles LM,de Araujo ML,Endringer DC,Fronza M,Scherer R

    更新日期:2019-10-01 00:00:00

  • WITHDRAWN: Selection of a surrogate beta-lactam testing agent for initial susceptibility testing of doripenem, a new carbapenem.

    abstract::This article has been withdrawn consistent with Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The Publisher apologizes for any inconvenience this may cause. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.09.009

    authors: Jones RN,Sader HS,Fritsche TR,Janechek MJ

    更新日期:2007-10-04 00:00:00

  • A two year global evaluation of the susceptibility of Candida species to fluconazole by disk diffusion.

    abstract::The in-vitro activity of fluconazole against 46,831 yeast isolates collected over a two-year period from 57 laboratories in 33 countries worldwide was assessed using a disc diffusion method. Candida albicans was the organism isolated most frequently, accounting for 68.6% of the total number of isolates. C. glabrata, C...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(01)00243-7

    authors: Liebowitz LD,Ashbee HR,Evans EG,Chong Y,Mallatova N,Zaidi M,Gibbs D,Global Antifungal Surveillance Group.

    更新日期:2001-05-01 00:00:00

  • LEADER surveillance program results for 2006: an activity and spectrum analysis of linezolid using clinical isolates from the United States (50 medical centers).

    abstract::Surveillance for emerging linezolid resistance among commonly occurring Gram-positive pathogens in the United States began with the 2002 ZAAPS program and more recently (2004) expanded as the LEADER program. The 2006 LEADER program processed 5374 strains from 50 medical centers (100 per site) located within the 9 US c...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,多中心研究

    doi:10.1016/j.diagmicrobio.2007.06.004

    authors: Jones RN,Fritsche TR,Sader HS,Ross JE

    更新日期:2007-11-01 00:00:00

  • Diagnostic value of an enzyme-linked immunospot assay for interferon-γ in genitourinary tuberculosis.

    abstract::The aim of this study was to evaluate the diagnostic performance of an enzyme-linked immunospot (ELISPOT) assay for interferon-γ in patients with suspected genitourinary tuberculosis (TB). A total of 30 patients with suspected genitourinary TB at the National Taiwan University Hospital, Taipei, Taiwan, were prospectiv...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.07.006

    authors: Lai CC,Tan CK,Lin SH,Liao CH,Huang YT,Wang CY,Wang JY,Lin HI,Hsueh PR

    更新日期:2010-11-01 00:00:00

  • Immunoenzymatic test for Q-fever.

    abstract::An immunoenzymatic test using as antigen purified suspensions of Coxiella burnetti coated by methylglyoxal on microtiter plates was developed. Multiple testing of the same sera gave similar results: two dilutions of serum (1:400 and 1:1600) were used in routine tests. Good agreement between the immunoenzymatic and the...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(86)90146-x

    authors: Roges G,Edlinger E

    更新日期:1986-02-01 00:00:00

  • Activity of fosfomycin when tested against US contemporary bacterial isolates.

    abstract::Fosfomycin and comparators were susceptibility tested against over 2200 contemporary clinical isolates from US medical centers. Fosfomycin was active against Enterobacterales (MIC50/90, 4/16 μg/mL), including multidrug-resistant isolates. Potent activity was exhibited against gram-positive organisms, including Staphyl...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.08.010

    authors: Flamm RK,Rhomberg PR,Watters AA,Sweeney K,Ellis-Grosse EJ,Shortridge D

    更新日期:2019-02-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract::This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.08.006

    authors: Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期:2008-02-01 00:00:00

  • Helicobacter pylori growth stimulation by adrenaline detected by two methods.

    abstract::Our aim was to assess growth effects of adrenaline on 32 Helicobacter pylori strains on 2.5% blood agar. By agar-well diffusion and disk diffusion methods, concentration-dependent growth stimulation occurred in ≤50.0% and 36.8% of the strains, respectively. Growth inhibition was less frequent. Most strains were adrena...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.08.004

    authors: Boyanova L,Markovska R,Mitov I

    更新日期:2019-01-01 00:00:00

  • Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs.

    abstract::Resistance to linezolid among Enterococcus faecium and Enterococcus faecalis isolates has been reported in patients who receive a prolonged course of the drug. We report two cases of linezolid-resistant Enterococcus faecalis that occurred in patients who previously received linezolid for infections with vancomycin-res...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(03)00153-6

    authors: Ruggero KA,Schroeder LK,Schreckenberger PC,Mankin AS,Quinn JP

    更新日期:2003-11-01 00:00:00

  • Four year prospective evaluation of nosocomial bacteremia: epidemiology, microbiology, and patient outcome.

    abstract::A prospective study of all patients with clinically significant nosocomial bacteremia at one institution from 1994 to 1997 was performed to: (1) describe the epidemiology and microbiology of nosocomial bacteremias; (2) determine the crude mortality associated with such infections; and (3) identify independent predicto...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(00)00192-9

    authors: Lark RL,Chenoweth C,Saint S,Zemencuk JK,Lipsky BA,Plorde JJ

    更新日期:2000-11-01 00:00:00

  • Group-C beta-hemolytic streptococcal bacteremia.

    abstract::Group-C beta-hemolytic streptococci (GCBHS) is an uncommon cause of bacteremia. In a 5-year period, GCBHS accounted for 0.28% of positive blood cultures and 0.35% of bacteremias documented at our hospital. The incidence of GCBHS bacteremia was 0.05 episodes per 1000 admissions. We were able to analyze clinical data of...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90040-z

    authors: Berenguer J,Sampedro I,Cercenado E,Baraia J,Rodríguez-Créixems M,Bouza E

    更新日期:1992-02-01 00:00:00

  • Loop-mediated isothermal amplification (LAMP) for the rapid detection of Mycoplasma genitalium.

    abstract::Mycoplasma genitalium is a sexually transmissible, pathogenic bacterium and a significant cause of nongonococcal urethritis in both men and women. Due to the difficulty of the culture of M. genitalium from clinical samples, the laboratory diagnosis of M. genitalium infection is almost exclusively carried out using nuc...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.05.010

    authors: Edwards T,Burke P,Smalley HB,Gillies L,Longhurst D,Vipond B,Hobbs G

    更新日期:2015-09-01 00:00:00

  • In vitro activity of antituberculous agents against Mycobacterium tuberculosis isolates from Bogota, DC (Colombia) evaluated by the ETest.

    abstract::Tuberculosis tests for antimicrobial susceptibility takes weeks. However, delayed therapy, can compromise the patient, as well as lead to an increase in disease incidence. Among infectious diseases, tuberculosis continues to be a leading cause of death in the world. The E-test is a new concept for Minimal Inhibitory C...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(99)00068-1

    authors: Sánchez L,Londoño D,Arango AI,Mattar S

    更新日期:1999-10-01 00:00:00

  • Optimal cefotaxime dosing for gram-negative bacteremia: effective trough serum bactericidal titers and drug concentrations 8 and 12 hr after 1- or 2-gm infusions.

    abstract::Thirteen adult patients (47-81 yr) with gram-negative bacteremia and normal (less than or equal to 1.5 mg/dl) serum creatinines were treated with 1 or 2 gm of cefotaxime every 8 or 12 hr. The infecting organisms were Escherichia coli (9 strains), Klebsiella pneumoniae (2 strains), and one isolate of Salmonella enterit...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(88)90102-2

    authors: Goodpasture HC,Gerlach EH,Jones RN,Peterie JD

    更新日期:1988-02-01 00:00:00

  • Pharmacokinetics of cefotaxime in healthy volunteers and patients.

    abstract::Cefotaxime is a third-generation cephalosporin that has maintained good susceptibility pattern despite its extensive use. It is available for intravenous and intramuscular administration. Its pharmacokinetic property includes a small volume of distribution with low protein binding. Cefotaxime's half-life is approximat...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00072-i

    authors: Patel KB,Nicolau DP,Nightingale CH,Quintiliani R

    更新日期:1995-05-01 00:00:00

  • Mycetoma of the scalp due to Microsporum canis: hystopathologic, mycologic, and immunogenetic features in a 6-year-old girl.

    abstract::Dermatophytic mycetoma is an extremely rare subcutaneous mycosis. Here, we report the case of a 6-year-old girl with clinical, histologic, and mycologic findings consistent with a mycetoma of the scalp caused by Microsporum canis. To our knowledge, this is the first report showing the immunologic and immunogenetic fea...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2011.02.003

    authors: Chiapello LS,Dib MD,Nuncira CT,Nardelli L,Vullo C,Collino C,Abiega C,Cortes PR,Spesso MF,Masih DT

    更新日期:2011-05-01 00:00:00

  • Clinically expedient reporting of rapid diagnostic test information.

    abstract::With the development of rapid diagnostic tests in the clinical microbiology laboratory has come an awareness of the importance of rapid results reporting. Clearly, the potential clinical impact of rapid diagnostic tests is dependent on expeditious reporting. Traditional manual reporting systems are encumbered by the n...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(86)80053-0

    authors: Doern GV

    更新日期:1986-03-01 00:00:00

  • Reproducibility of gemifloxacin and comparison fluoroquinolone MIC results using Sensititre commercial dry-form panels.

    abstract::This study examined the ability of commercial dry-form broth microdilution panels (Sensititre, TREK Diagnostics, Cleveland, OH) to produce accurate minimum inhibitory concentration (MIC) results for gemifloxacin, a new fluoroquinolone. Reproducibility for gemifloxacin was 100.0% for on-scale values within +/-1 log2 di...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2004.11.005

    authors: Ross JE,Sader HS,Fritsche TR,Jones RN

    更新日期:2005-03-01 00:00:00

  • In vitro susceptibility of 33 clinical case isolates of Xanthomonas maltophilia. Inconsistent correlation of agar dilution and of disk diffusion test results.

    abstract::Xanthomonas maltophilia is an emerging nosocomial pathogen, possibly selected by a changing antimicrobial usage and patient population. In the present study, we tested the susceptibility of 33 recent clinical case isolates to 12 commonly employed antimicrobials. Trimethoprim-sulfamethoxazole (1:19 ratio) and doxycycli...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(91)90072-n

    authors: Hohl P,Frei R,Aubry P

    更新日期:1991-09-01 00:00:00

  • Detection of herpes simplex virus in clinical specimens by DNA hybridization.

    abstract::An assay to detect herpes simplex virus (HSV) DNA in clinical specimens has been developed. It utilizes nucleic acid hybridization with a 32P-labeled DNA probe prepared from a fragment of HSV DNA cloned in a plasmid vector. This assay can detect 5 X 10(4) plaque-forming units of cell-free HSV and as few as four virus-...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(83)90041-x

    authors: Redfield DC,Richman DD,Albanil S,Oxman MN,Wahl GM

    更新日期:1983-06-01 00:00:00

  • Risk factors for acquisition of vancomycin-resistant enterococci among hematology-oncology patients.

    abstract::The incidence of VRE has increased dramatically and hematology-oncology patients are at high risk for acquisition of colonization and development of infection. Therefore, we performed a prospective cohort study to determine risk factors for VRE acquisition among hematology-oncology patients. Patients admitted to a sin...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00392-9

    authors: Suntharam N,Lankford MG,Trick WE,Peterson LR,Noskin GA

    更新日期:2002-07-01 00:00:00

  • Cross-reactive monoclonal antibodies raised against the lipopolysaccharide antigen of Salmonella minnesota Re chemotype: diagnostic relevance.

    abstract::Three cross-reactive monoclonal antibodies (MAbs) of IgM and IgG2b isotype were generated in two separate fusions after immunization of BALB/c mice with heat killed Salmonella minnesota R595 of Re chemotype and acid-treated bacteria, coated with Re lipopolysaccharide (LPS) antigen. The specificity of the MAbs was demo...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(02)00545-x

    authors: Mitov I,Haralambieva I,Petrov D,Ivanova R,Kamarinchev B,Iankov I

    更新日期:2003-04-01 00:00:00

  • More potency assay results for generic non-USA lots of piperacillin/tazobactam and initial reports for generic meropenem compounds marketed in the USA.

    abstract::An ongoing program of international generic antimicrobial potency assays for piperacillin/tazobactam has been summarized here through December 2010, and the initial results for meropenem generic lots from the United States are also presented. Fifteen additional piperacillin/tazobactam generic lots revealed an average ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.01.024

    authors: Jones RN,Sader HS,Flamm RK,Watters AA

    更新日期:2013-05-01 00:00:00

  • Optimization of LipL32 PCR assay for increased sensitivity in diagnosing leptospirosis.

    abstract::Early diagnosis of leptospirosis in humans is critical with regard to initiation of appropriate treatment; however, the gold standard serological test cannot detect antibodies until nearly a week after symptom onset. PCR has been shown to be sensitive and specific in the early phase of leptospirosis. Previously, we de...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.03.024

    authors: Galloway RL,Hoffmaster AR

    更新日期:2015-07-01 00:00:00