当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load.

Collaborative national multicenter for the identification of conversion factors from copies/mL to international units/mL for the normalization of HCMV DNA load.

Abstract：

:The present multicentric (n = 11 laboratories) study aimed to identify conversion factors from copies/mL to international units (IU)/mL for the normalization of HCMV DNA load using the first WHO International Standard for HCMV nucleic acid amplification techniques and to enhance interlaboratory agreement of HCMV DNA quantification methods. Study protocols for whole blood and plasma (extraction and amplification) were performed to calculate conversion factors from HCMV DNA copy number to IU. The greatest variability was observed in samples with lower HCMV concentrations (3.0 Log10) in both biological matrices. Overall, 73.1% (206/282) of whole blood and 82.2% (324/394) of plasma samples analyzed fell within an acceptable variation range (±0.5 Log10 difference). An average of 0.64 (range 0.21-1.17) was the conversion factor calculated for the HCMV whole blood panel and 0.82 (range 0.39-2.2) for the HCMV plasma panel.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Sidoti F,Piralla A,Costa C,Scarasciulli ML,Calvario A,Conaldi PG,Paba P,Perno CF,Gaeta A,Antonelli G,Sodano G,Santangelo R,Sanguinetti M,Vatteroni ML,Barzon L,Palù G,Abbate I,Capobianchi MR,Piccirilli G,Lazzarotto T

doi

10.1016/j.diagmicrobio.2019.05.012

subject

Has Abstract

pub_date

2019-10-01 00:00:00

pages

152-158

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30703-X

journal_volume

95

pub_type

杂志文章,多中心研究

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