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Evaluation of a sequential enzyme immunoassay testing algorithm for Lyme disease demonstrates lack of test independence but high diagnostic specificity.

Abstract：

:To diagnose Lyme disease, a two-tier testing algorithm is used in which supplemental IgM and IgG immunoblots to detect antibody to Borrelia burgdorferi are reflexively performed if a first-tier assay, such as a whole-cell sonicate-based enzyme immunoassay (WCS EIA), is reactive. Recent data suggest that equal specificity is found by substituting the C6 peptide EIA for immunoblots. In this study using 3956 control sera, we demonstrated that although this two-tier testing algorithm does significantly improve diagnostic specificity compared with each of the EIAs individually, the WCS EIA and the C6 peptide EIA are not independent tests. Therefore, when the C6 peptide EIA is used as the second-tier test, it should be regarded as a supplemental rather than a confirmatory test.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Wormser GP,Molins CR,Levin A,Lipsett SC,Nigrovic LE,Schriefer ME,Branda JA

doi

10.1016/j.diagmicrobio.2018.02.006

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

217-219

issue

3

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30053-1

journal_volume

91

pub_type

杂志文章

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