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Detection of enteroviruses using subgenomic probes of Coxsackie virus B4 by hybridization.

Abstract：

:The objective of this research was to develop group- and type-specific probes for the detection of enteroviruses. Coxsackie virus B4 RNA was cloned, and a series of subgenomic clones were generated. Six of these clones, containing sequences from the 3' end or the 5' end of the genome, were tested for their ability to detect these viruses in a small number of infected cells employing nucleic acid hybridization technique and total cytoplasmic RNA from a panel of 11 serotypes of enteroviruses. The RNA from cells infected with Coxsackie B viruses gave characteristic and positive hybridization signals. Coxsackie B-specific probes and a control Echo 9 probe detected Coxsackie A9 and Echo 3 weakly. As little as 0.5 microgram of the RNA--which contained 10-20 ng of poly(A)-containing, virus-specific, hybridizable RNA--was sufficient to successfully conduct the assay, suggesting high sensitivity of these probes. Probes that are 3' end-specific appear to be group specific, while those that are 5' end-specific appear to be type specific among the serotypes tested.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Chatterjee NK,Kaehler M,Deibel R

doi

10.1016/0732-8893(88)90014-4

subject

Has Abstract

pub_date

1988-11-01 00:00:00

pages

129-36

issue

3

eissn

0732-8893

issn

1879-0070

pii

0732-8893(88)90014-4

journal_volume

11

pub_type

杂志文章

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