当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > Impact of inter-genotypic recombination and probe cross-reactivity on the performance of the Abbott RealTime HCV Genotype II assay for hepatitis C genotyping.

Impact of inter-genotypic recombination and probe cross-reactivity on the performance of the Abbott RealTime HCV Genotype II assay for hepatitis C genotyping.

Abstract：

:The Abbott RealTime HCV Genotype II assay (Abbott-RT-HCV assay) is a real-time PCR based genotyping method for hepatitis C virus (HCV). This study measured the impact of inter-genotypic recombination and probe cross-reactivity on the performance of the Abbott-RT-HCV assay. 517 samples were genotyped using the Abbott-RT-HCV assay over a one-year period, 34 (6.6%) were identified as HCV genotype 1 without further subtype designation raising the possibility of inaccurate genotyping. These samples were subjected to confirmatory sequencing. 27 of these 34 (79%) samples were genotype 1b while five (15%) were genotype 6. One HCV isolate was an inter-genotypic 1a/4o recombinant. This is a novel natural HCV recombinant that has never been reported. Inter-genotypic recombination and probe cross-reactivity can affect the accuracy of the Abbott-RT-HCV assay, both of which have significant implications on antiviral regimen choice. Confirmatory sequencing of ambiguous results is crucial for accurate genotyping.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Sridhar S,Yip CCY,Chan JFW,To KKW,Cheng VCC,Yuen KY

doi

10.1016/j.diagmicrobio.2017.12.024

subject

Has Abstract

pub_date

2018-05-01 00:00:00

pages

34-37

issue

1

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(18)30011-7

journal_volume

91

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • rpoB gene high-resolution melt curve analysis: a rapid approach for diagnosis and screening of drug resistance in tuberculous meningitis.

    abstract：:Timely and rapid diagnosis of multidrug resistance in tuberculous meningitis (TBM) is a challenge both for a microbiologist and neurologist. The present study was conducted to evaluate role of real-time polymerase chain reaction (PCR) using rpoB, IS6110, and MPB64 as targets in diagnosis of TBM in 110 patients and sub...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.06.010

    authors： Sharma K,Modi M,Kaur H,Sharma A,Ray P,Varma S

    更新日期：2015-10-01 00:00:00

  • Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs.

    abstract：:Resistance to linezolid among Enterococcus faecium and Enterococcus faecalis isolates has been reported in patients who receive a prolonged course of the drug. We report two cases of linezolid-resistant Enterococcus faecalis that occurred in patients who previously received linezolid for infections with vancomycin-res...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00153-6

    authors： Ruggero KA,Schroeder LK,Schreckenberger PC,Mankin AS,Quinn JP

    更新日期：2003-11-01 00:00:00

  • The use of biotyping and DNA fingerprinting in typing Candida albicans from hospitalized patients.

    abstract：:The application of typing procedures for the purpose of strain differentiation among isolates of Candida albicans obtained from hospitalized patients has been limited. We have applied biotyping and DNA restriction fragment analysis (DNA fingerprinting) by using EcoRI to the study of C. albicans isolates obtained from ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(90)90080-f

    authors： Pfaller MA,Cabezudo I,Hollis R,Huston B,Wenzel RP

    更新日期：1990-11-01 00:00:00

  • In vitro activity of the combination of trovafloxacin and other antibiotics against enterococci.

    abstract：:The activities of trovafloxacin and ciprofloxacin against 38 strains of non-beta-lactamase-producing enterococci, resistant to ampicillin, 34 strains susceptible to ampicillin, and 3 vancomycin-resistant enterococci were studied. Trovafloxacin was more active than ciprofloxacin against all the enterococci studied. The...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(97)00161-2

    authors： Schick DG,Canawati HN,Montgomerie JZ

    更新日期：1997-12-01 00:00:00

  • Reproducibility of positive results for rare pathogens on the FilmArray GI Panel.

    abstract：:Though the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for selected targets were ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.03.013

    authors： Hitchcock MM,Hogan CA,Budvytiene I,Banaei N

    更新日期：2019-09-01 00:00:00

  • Ceftobiprole activity when tested against contemporary bacteria causing bloodstream infections in the United States (2016-2017).

    abstract：:Ceftobiprole medocaril, the prodrug of ceftobiprole, is an advanced-generation cephalosporin that is approved in many European and non-European countries for the treatment of adults with hospital-acquired pneumonia (excluding ventilator-associated pneumonia) and community-acquired pneumonia and is currently being eval...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.01.015

    authors： Pfaller MA,Flamm RK,Duncan LR,Shortridge D,Smart JI,Hamed KA,Mendes RE,Sader HS

    更新日期：2019-07-01 00:00:00

  • New findings about trichodysplasia spinulosa-associated polyomavirus (TSPyV)--novel qPCR detects TSPyV-DNA in blood samples.

    abstract：:A new real-time PCR assay for trichodysplasia spinulosa-associated polyomavirus (TSPyV) DNA detection was designed, and blood samples from kidney transplant recipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2015.10.011

    authors： Urbano PR,Nali LH,Bicalho CS,Pierrotti LC,David-Neto E,Pannuti CS,Romano CM

    更新日期：2016-02-01 00:00:00

  • Characteristics and outcomes of Fusobacterium nucleatum bacteremia--a 6-year experience at a tertiary care hospital in northern Taiwan.

    abstract：:Fusobacterium nucleatum bacteremia is critical and not well defined. To identify the clinical characteristics and outcomes, we conducted a retrospective review of hospitalized patients from January 2004 to December 2009 at a tertiary center in northern Taiwan. Fifty-seven patients were enrolled. The mean age was 58.1 ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2010.12.017

    authors： Yang CC,Ye JJ,Hsu PC,Chang HJ,Cheng CW,Leu HS,Chiang PC,Lee MH

    更新日期：2011-06-01 00:00:00

  • In vitro activity of garenoxacin tested against a worldwide collection of ciprofloxacin-susceptible and ciprofloxacin-resistant Enterobacteriaceae strains (1999-2004).

    abstract：:Garenoxacin is a novel des-F(6)-quinolone with a chemical structure that lacks the C6 position fluorine and has a unique difluoromethoxy substitution at position C8. This study evaluated the in vitro activity of garenoxacin tested against a large collection of Enterobacteriaceae collected worldwide. The bacterial isol...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2006.12.006

    authors： Sader HS,Fritsche TR,Jones RN

    更新日期：2007-05-01 00:00:00

  • Pharmacokinetic/pharmacodynamic profile for tigecycline-a new glycylcycline antimicrobial agent.

    abstract：:Tigecycline is a new first-in-class glycylcycline antimicrobial agent with expanded broad-spectrum activity against both Gram-negative and Gram-positive aerobes and anaerobes, as well as atypical bacterial species. The spectrum of activity extends to clinically relevant susceptible and multidrug-resistant strains of S...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/j.diagmicrobio.2005.05.006

    authors： Meagher AK,Ambrose PG,Grasela TH,Ellis-Grosse EJ

    更新日期：2005-07-01 00:00:00

  • Mycobacterial identification by computer-aided gas-liquid chromatography.

    abstract：:The suitability of the Microbial Identification System (MIS) marketed by Microbial ID (Newark, DE, USA) for routine diagnosis of clinically important mycobacteria was investigated and assessed. Cellular fatty acids were extracted from 1077 stock and recent clinical isolates. They were analyzed using a gas-liquid chrom...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(94)90117-1

    authors： Smid I,Salfinger M

    更新日期：1994-06-01 00:00:00

  • Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples.

    abstract：:Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and the mip gene was targeted for ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.05.004

    authors： Nazarian EJ,Bopp DJ,Saylors A,Limberger RJ,Musser KA

    更新日期：2008-10-01 00:00:00

  • In vitro antimicrobial activity of moxifloxacin compared to other quinolones against recent clinical bacterial isolates from hospitalized and community-based cancer patients.

    abstract：:The in vitro spectrum of moxifloxacin (a C-8-methoxyquinolone) was compared to that of ciprofloxacin and levofloxacin against 924 recent clinical isolates from cancer patients. Moxifloxacin was more active than the comparator agents against Gram-positive pathogens, with potent activity against Aerococcus spp., Listeri...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00115-9

    authors： Rolston KV,Frisbee-Hume S,LeBlanc B,Streeter H,Ho DH

    更新日期：2003-10-01 00:00:00

  • Diagnosis of pneumococcal empyema using immunochromatographic test on pleural fluid and serotype distribution in Korean children.

    abstract：:To evaluate the diagnostic value of immunochromatographic test (ICT) on pleural fluid in diagnosing pneumococcal empyema in children and to determine pneumococcal serotypes, 62 exudative parapneumonic effusions from Korean children were tested with culture, ICT for S. pneumoniae, pneumococcal autolysin polymerase chai...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.09.025

    authors： Lee JH,Kim SH,Lee J,Choi EH,Lee HJ

    更新日期：2012-02-01 00:00:00

  • Group-C beta-hemolytic streptococcal bacteremia.

    abstract：:Group-C beta-hemolytic streptococci (GCBHS) is an uncommon cause of bacteremia. In a 5-year period, GCBHS accounted for 0.28% of positive blood cultures and 0.35% of bacteremias documented at our hospital. The incidence of GCBHS bacteremia was 0.05 episodes per 1000 admissions. We were able to analyze clinical data of...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90040-z

    authors： Berenguer J,Sampedro I,Cercenado E,Baraia J,Rodríguez-Créixems M,Bouza E

    更新日期：1992-02-01 00:00:00

  • Species identification of Streptococcus bovis group isolates causing bacteremia: a comparison of two MALDI-TOF MS systems.

    abstract：:This study compared two MALDI-TOF MS systems (Biotyper and VITEK MS) on clinical Streptococcus bovis group isolates (n=66). The VITEK MS gave fewer misidentifications and a higher rate of correct identifications than the Biotyper. Only the identification of S. lutetiensis by the VITEK MS was reliable. Additional optim...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.02.007

    authors： Agergaard CN,Knudsen E,Dargis R,Nielsen XC,Christensen JJ,Justesen US

    更新日期：2017-05-01 00:00:00

  • Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay.

    abstract：:The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ-producing cell...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.09.015

    authors： Kim S,Kim YK,Lee H,Cho JE,Kim HY,Uh Y,Kim YM,Kim H,Cho SN,Jeon BY,Lee H

    更新日期：2013-01-01 00:00:00

  • Oral ofloxacin for infections caused by bacteria resistant to oral antimicrobial agents.

    abstract：:We conducted an open trial of oral ofloxacin, 400 mg q 12 hr, in the treatment of infections caused by Pseudomonas aeruginosa and other bacteria resistant to traditional oral antibiotics. There were 53 evaluable subjects, 30 having infection of the skin/skin structure, 13 of the respiratory tract, six of bone, and fou...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 临床试验,杂志文章

    doi：10.1016/0732-8893(92)90085-8

    authors： Eron LJ,Gentry LO

    更新日期：1992-07-01 00:00:00

  • Activity of meropenem and comparators against Pseudomonas aeruginosa and Acinetobacter spp. isolated in the MYSTIC Program, 2002-2004.

    abstract：:This study examines the susceptibilities of meropenem and other broad-spectrum antimicrobials tested against bacterial isolates collected from hospitalized patients during 2002-2004 from worldwide medical centers participating in the Meropenem Yearly Susceptibility Test Information Collection (MYSTIC) Program. The in ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.10.002

    authors： Unal S,Garcia-Rodriguez JA

    更新日期：2005-12-01 00:00:00

  • Evaluation of the in vitro activity of cefepime compared to other broad-spectrum cephalosporins against clinical isolates from eighteen Brazilian hospitals by using the Etest.

    abstract：:The in vitro activity of cefepime was compared to that of ceftazidime, ceftriaxone, and cefotaxime in a multicenter study involving 10 clinical microbiology laboratories and clinical isolates from 18 Brazilian hospitals from 7 cities (4 states). A total of 982 isolates consecutively collected between December 1995 and...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(97)00015-1

    authors： Sader HS,Mimiça I,Rossi F,Zoccoli C,Montelli AC,Sampaio JL,Segura AJ,Magalhães M,Nowakonski A,Mendes CM

    更新日期：1997-06-01 00:00:00

  • Tigecycline in vitro activity against commonly encountered multidrug-resistant gram-negative pathogens in a Middle Eastern country.

    abstract：:The lack of data from the Middle East warranted studying tigecycline in vitro activity in Lebanon against consecutive multidrug-resistant (MDR) bacteria, including extended-spectrum beta-lactamases producing clinical isolates of Escherichia coli (n = 150), Klebsiella pneumoniae (n = 100), and Acinetobacter spp. (n = 6...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.08.011

    authors： Araj GF,Ibrahim GY

    更新日期：2008-12-01 00:00:00

  • Evaluation of VITEK 2, MicroScan, and Phoenix for identification of clinical isolates and reference strains.

    abstract：:To compare the identification accuracies of VITEK 2 (bioMérieux), MicroScan (Siemens Healthcare), and Phoenix (Becton Dickinson), microbial identification was performed on 160 clinical isolates and 50 reference strains on each of these 3 systems, using the appropriate identification kit provided by each system. Of the...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.04.013

    authors： Jin WY,Jang SJ,Lee MJ,Park G,Kim MJ,Kook JK,Kim DM,Moon DS,Park YJ

    更新日期：2011-08-01 00:00:00

  • Two-year surveillance on fluconazole susceptibility of Candida spp isolates in a general and university hospital in Rome.

    abstract：:Fluconazole susceptibility was tested in 385 clinical yeast isolates (285 Candida albicans, 38 C. glabrata, 31 C. tropicalis, 31 other Candida subsp.) using the agar disk diffusion test. Yeasts were collected from specimens obtained from outpatients (69) and inpatients (intensive care unit: 79 isolates, major burn uni...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00280-2

    authors： Testore GP,Falco F,Sarrecchia C,Sordillo P,Bontempo G,Andreoni M

    更新日期：2001-09-01 00:00:00

  • Multi-laboratory evaluation of the rapid genoserotyping array (SGSA) for the identification of Salmonella serovars.

    abstract：:Salmonella serotyping is an essential first step for identification of isolates associated with disease outbreaks. The Salmonella genoserotyping array (SGSA) is a microarray-based alternative to standard serotyping designed to rapidly identify 57 of the most commonly reported serovars through detection of the genes en...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2014.08.006

    authors： Yoshida C,Lingohr EJ,Trognitz F,MacLaren N,Rosano A,Murphy SA,Villegas A,Polt M,Franklin K,Kostic T,OIE Reference Laboratory for Salmonellosis.,Austrian Agency for Health and Food Safety.,Kropinski AM,Card RM

    更新日期：2014-11-01 00:00:00

  • Host and microbiologic factors associated with mortality in Taiwanese children with invasive pneumococcal diseases, 2001 to 2006.

    abstract：:We concurrently evaluated host- and organism-related factors in determining the outcomes of 160 invasive pneumococcal diseases episodes in 158 Taiwanese children during 2001 and 2006. Most (138/160, 86.2%) episodes occurred at age less than 60 months, and an underlying condition was present in 35 (22.2%) cases. Common...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.10.006

    authors： Chen CJ,Lin CL,Chen YC,Wang CW,Chiu CH,Lin TY,Huang YC

    更新日期：2009-02-01 00:00:00

  • Low-level resistance to glycopeptides amongst staphylococcus species: surveillance in a university hospital and evaluation of a vancomycin screening agar.

    abstract：:The prevalence of low-level resistance to glycopeptides (teicoplanin MIC > or = 8 microg/mL and vancomycin MIC > or = 4 microg/mL) among staphylococci was investigated over a 15 month period. A total of 2,279 isolates (1,519 S. aureus, 760 coagulase-negative staphylococcus (CNS)) were screened using inoculum of 10(6) ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00302-9

    authors： Park YJ,Jun Park J,Lee SO,Oh EJ,Kee Kim B

    更新日期：2001-11-01 00:00:00

  • Change in the clinical antifungal sensitivity profile of Aspergillus flavus induced by azole and a benzimidazole fungicide exposure.

    abstract：:The study evaluated the change in the clinical antifungal sensitivity profile of A. flavus strains after exposure to azole and benzimidazole fungicide. Exposure to fungicide altered the sensitivity profile for the antifungal itraconazole, voriconazole and posaconazole. This change was characterized by an increase in t...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.05.019

    authors： Meireles LM,de Araujo ML,Endringer DC,Fronza M,Scherer R

    更新日期：2019-10-01 00:00:00

  • Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA).

    abstract：:A biotinylated single-tube nested polymerase chain reaction (PCR) assay with microwell hybridization assay (bPCR-ELISA) was developed for detection of Mycobacterium tuberculosis in clinical specimens. A total of 659 specimens (601 respiratory specimens and 58 nonrespiratory specimens) were collected for evaluation usi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2003.11.006

    authors： Yam WC,Cheng VC,Hui WT,Wang LN,Seto WH,Yuen KY

    更新日期：2004-04-01 00:00:00

  • Evaluation of two commercial microtiter cytotoxin assays for the detection of Clostridium difficile toxin B in stool specimens.

    abstract：:Two commercial microtiter cytotoxin assays using a fibroblast cell line (Bartels, Baxter Diagnostics, Inc., Deerfield, IL) and an epithelial cell line (Cytotoxi Test, Advanced Clinical Diagnostics, Toledo, OH) were evaluated for their ability to detect Clostridium difficile toxin B in stool specimens. After 48 hours, ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(96)00172-1

    authors： Fedorko DP,Williams EC

    更新日期：1996-09-01 00:00:00

  • Comparison of five different susceptibility test methods for detecting antimicrobial agent resistance among Haemophilus influenzae isolates.

    abstract：:The detection of antimicrobial agent resistance among ninety-eight Haemophilus influenzae isolates was assessed by six different antibiotic test methods: agar dilution on Mueller-Hinton agar supplemented with 5% lysed horse blood (MH-LHB), E-test using both Haemophilus test medium (HTM) agar and chocolate Mueller-Hint...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(96)00026-0

    authors： Giger O,Mortensen JE,Clark RB,Evangelista A

    更新日期：1996-03-01 00:00:00