当前位置： SCI文献检索 > DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE期刊下所有文献 > The use of biotyping and DNA fingerprinting in typing Candida albicans from hospitalized patients.

The use of biotyping and DNA fingerprinting in typing Candida albicans from hospitalized patients.

Abstract：

:The application of typing procedures for the purpose of strain differentiation among isolates of Candida albicans obtained from hospitalized patients has been limited. We have applied biotyping and DNA restriction fragment analysis (DNA fingerprinting) by using EcoRI to the study of C. albicans isolates obtained from hospitalized patients. A total of 68 isolates from 15 patients were studied. Thirteen subtypes were identified by biotyping, 8 by DNA fingerprinting, and 21 by a combination of the biotyping and DNA fingerprinting approaches (composite subtype). Both techniques were highly reproducible. In examining the strain variation among isolates obtained from multiple anatomic sites over time, we found that similar, if not identical, strains were recovered from the oropharynx, urine, stool, and blood in a given patient, and these strains persisted. Only rarely did two patients share the same composite subtype suggesting sporadic nosocomial transmission. The combination of biotyping and DNA fingerprinting improved strain discrimination compared to either method alone. Further investigation with these and other epidemiologic typing methods will be necessary to enhance the understanding of the epidemiology and pathogenesis of candidiasis in hospitalized patients.

journal_name

Diagn Microbiol Infect Dis

journal_title

Diagnostic microbiology and infectious disease

authors

Pfaller MA,Cabezudo I,Hollis R,Huston B,Wenzel RP

doi

10.1016/0732-8893(90)90080-f

subject

Has Abstract

pub_date

1990-11-01 00:00:00

pages

481-9

issue

6

eissn

0732-8893

issn

1879-0070

pii

0732-8893(90)90080-F

journal_volume

13

pub_type

杂志文章

相关文献

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE文献大全
  • A simple method to prepare Salmonella (A-E) polyvalent antiserum.

    abstract：:The preparation of Salmonella O serogroup (A-I) polyvalent antiserum is laborious. In an alternate method, rabbit antiserum raised to cell surface proteins of Salmonella serotype typhi was compared with a commercial Salmonella polyvalent (A-I) antiserum; 51 Salmonella (A-E) and 16 strains of related organisms tested g...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(93)90133-r

    authors： Sridharan G,Jesudason MV,Santhosh M,John TJ

    更新日期：1993-01-01 00:00:00

  • Comparison of Gram and Kopeloff stains in the diagnosis of bacterial vaginosis in pregnancy.

    abstract：:Bacterial vaginosis (BV) is commonly diagnosed by using the Nugent score, a semiquantitative scoring system to evaluate bacterial morphotypes on Gram stain of vaginal secretions. Some authors have suggested using the Kopeloff modification of the Gram stain. Asymptomatic BV in pregnancy has been associated with adverse...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2005.09.017

    authors： Libman MD,Kramer M,Platt R,Montreal Prematurity Study Group.

    更新日期：2006-03-01 00:00:00

  • Antimicrobial susceptibility of Streptococcus pyogenes in Central, Eastern, and Baltic European Countries, 2005 to 2006: the cefditoren surveillance program.

    abstract：:The in vitro activity of penicillin, ampicillin, cefditoren, cefotaxime, erythromycin, clarithromycin, and levofloxacin against 763 clinical isolates of Streptococcus pyogenes was determined. Clinically significant isolates collected from November 2005 to December 2006 in the Czech Republic, Slovakia, Hungary, Poland,...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.12.018

    authors： Gracia M,Díaz C,Coronel P,Gimeno M,García-Rodas R,Rodríguez-Cerrato V,del Prado G,Huelves L,Ruiz V,Naves PF,Ponte MC,Granizo JJ,Soriano F

    更新日期：2009-05-01 00:00:00

  • Detection of enteroviruses using subgenomic probes of Coxsackie virus B4 by hybridization.

    abstract：:The objective of this research was to develop group- and type-specific probes for the detection of enteroviruses. Coxsackie virus B4 RNA was cloned, and a series of subgenomic clones were generated. Six of these clones, containing sequences from the 3' end or the 5' end of the genome, were tested for their ability to ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(88)90014-4

    authors： Chatterjee NK,Kaehler M,Deibel R

    更新日期：1988-11-01 00:00:00

  • Clinical characteristics of bacteremia caused by hypermucoviscous Klebsiella pneumoniae at a tertiary hospital.

    abstract：:This study aimed to assess the predictive factors of bacteremia due to hypermucoviscous Klebsiella pneumoniae (hvKP), as well as the mortality. The medical records of 114 patients with K. pneumoniae bacteremia who were divided into the hvKP (n = 24) and non-hvKP (n = 90) groups and were retrospectively reviewed. The m...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.04.008

    authors： Namikawa H,Yamada K,Sakiyama A,Imoto W,Yamairi K,Shibata W,Yoshii N,Niki M,Nakaie K,Oinuma KI,Tsubouchi T,Niki M,Tochino Y,Takemoto Y,Kaneko Y,Shuto T,Kakeya H

    更新日期：2019-09-01 00:00:00

  • Early cytokine and antibody responses against Coxiella burnetii in aerosol infection of BALB/c mice.

    abstract：:Coxiella burnetii, a Gram-negative intracellular bacterium, can give rise to Q fever in humans and is transmitted mainly by inhalation of infected aerosols from animal reservoirs. Serology is commonly used to diagnose Q fever, but the early cellular immune response-i.e., C. burnetii-specific interferon γ (IFN-γ) produ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2014.12.008

    authors： Schoffelen T,Self JS,Fitzpatrick KA,Netea MG,van Deuren M,Joosten LA,Kersh GJ

    更新日期：2015-04-01 00:00:00

  • Oral beta-lactams in the treatment of acute otitis media.

    abstract：:Acute otitis media (AOM) is an important infectious disease among children throughout the world. The management of AOM is complicated by difficulties in diagnosis, spontaneous resolution, altered microbiology after pneumococcal conjugate vaccine use, and expanding antimicrobial resistance. The recent introduction of t...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章,评审

    doi：10.1016/j.diagmicrobio.2007.01.011

    authors： Block SL,Doern GV,Pfaller MA

    更新日期：2007-03-01 00:00:00

  • Clinical application of viral cerebrospinal fluid PCR testing for diagnosis of central nervous system disorders: a retrospective 11-year experience.

    abstract：:The cerebrospinal fluid (CSF) polymerase chain reaction (PCR) is the gold standard to detect cerebral viral activity. As positive findings do not prove an impact on the neurological disorder, data interpretation is difficult. To better assess the impact of positive CSF PCR findings in different neurological diseases a...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2014.07.010

    authors： Kleines M,Scheithauer S,Schiefer J,Häusler M

    更新日期：2014-11-01 00:00:00

  • An advanced uracil DNA glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP) technique used in the sensitive and specific detection of Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in AIDS patients.

    abstract：:The rapid and accurate detection of Cryptosporidium spp. is critically important for the prevention and timely treatment of cryptosporidiosis in AIDS patients (APs). This study was conducted to examine a UDG-LAMP technique for the first time to diagnose cryptosporidiosis in APs. After collecting demographic and clinic...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.12.017

    authors： Fallahi S,Moosavi SF,Karimi A,Chegeni AS,Saki M,Namdari P,Rashno MM,Varzi AM,Tarrahi MJ,Almasian M

    更新日期：2018-05-01 00:00:00

  • Clinical utility of QuantiFERON-TB GOLD In-Tube and tuberculin skin test in patients with tuberculous pleural effusions.

    abstract：:Interferon gamma release assays are used for diagnosing latent tuberculosis (TB); however, their role in diagnosing tuberculous pleural effusion (TPE) is not defined. The aim of this study was to evaluate the usefulness of the QuantiFERON-TB Gold In-Tube assay (QFT-IT) and compare this assay with the tuberculin skin t...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2011.06.015

    authors： Chung JH,Han CH,Kim CJ,Lee SM

    更新日期：2011-11-01 00:00:00

  • Development of a rapid assay methodology for antimicrobial susceptibility testing of Staphylococcus aureus.

    abstract：:Development of a new phenotypic technique for rapid antimicrobial susceptibility testing (AST) of methicillin-resistant Staphylococcus aureus is presented. The new technique combines bacterial culturing and specific immunometric detection in a single separation-free process. The technique uses dry chemistry reagents a...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.07.007

    authors： Koskinen JO,Stenholm T,Vaarno J,Soukka J,Meltola NJ,Soini AE

    更新日期：2008-11-01 00:00:00

  • Effect of treatment of shell vial cell cultures with dimethyl sulfoxide and dexamethasone and age of MRC-5 monolayers for detection of cytomegalovirus.

    abstract：:Pretreatment of MRC-5 cell monolayers in commercially prepared shell vials with 1% dimethyl sulfoxide (DMSO) and 10(-5) mol/L dexamethasone (DEX) was evaluated. Preliminary experiments indicated enhanced infectivity of AD-169 for pretreated MRC-5 cells in shell vials of ages 9 and 16 days. Compared with untreated shel...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(90)90052-w

    authors： Fedorko DP,Ilstrup DM,Smith TF

    更新日期：1990-01-01 00:00:00

  • Design and implementation of a protocol for the detection of Legionella in clinical and environmental samples.

    abstract：:Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and the mip gene was targeted for ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2008.05.004

    authors： Nazarian EJ,Bopp DJ,Saylors A,Limberger RJ,Musser KA

    更新日期：2008-10-01 00:00:00

  • In vitro susceptibility of 33 clinical case isolates of Xanthomonas maltophilia. Inconsistent correlation of agar dilution and of disk diffusion test results.

    abstract：:Xanthomonas maltophilia is an emerging nosocomial pathogen, possibly selected by a changing antimicrobial usage and patient population. In the present study, we tested the susceptibility of 33 recent clinical case isolates to 12 commonly employed antimicrobials. Trimethoprim-sulfamethoxazole (1:19 ratio) and doxycycli...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(91)90072-n

    authors： Hohl P,Frei R,Aubry P

    更新日期：1991-09-01 00:00:00

  • Comparison of clindamycin, metronidazole, and penicillin for the treatment of experimental infections in mice caused by beta-lactamase-producing Bacteroides intermedius.

    abstract：:Turbid saline suspensions (0.35 ml, approximately 10(9) organisms) of a strain of beta-lactamase-producing Bacteroides intermedius were injected subcutaneously into the right groin of ICR male mice. Therapeutic intraperitoneal doses of clindamycin, metronidazole, or penicillin were administered 4 hr later, and thereaf...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(86)90088-x

    authors： Cockerill FR 3rd,Rosenblatt JE,Lee DT,Gay JD

    更新日期：1986-05-01 00:00:00

  • Urine D-arabinitol/L-arabinitol ratio in diagnosing Candida infection in patients with haematological malignancy and HIV infection.

    abstract：:Adult patients with hematologic malignancies along with HIV infected patients were prospectively studied to determine the performance of urine D-arabinitol/L-arabinitol (DA/LA) ratio in diagnosing invasive candidiasis. Ten evaluable febrile neutropenic patients had proven invasive candidiasis and elevated DA/LA ratios...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(01)00310-8

    authors： Eisen DP,Bartley PB,Hope W,Sigmundsdottir G,Pehrson C,Larsson L,Christensson B

    更新日期：2002-01-01 00:00:00

  • Identification of CTX-M beta-lactamases in Escherichia coli from hospitalized patients and residents of long-term care facilities.

    abstract：:Bacteria harboring CTX-M extended-spectrum beta-lactamases (ESBLs) have been identified worldwide, with most reports coming from regions outside North America. We have identified CTX-M enzymes in 31% of ESBL-positive Escherichia coli isolates from our hospital and more than half (53%) of the isolates from associated l...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2009.11.012

    authors： Urban C,Mariano N,Bradford PA,Tuckman M,Segal-Maurer S,Wehbeh W,Grenner L,Colon-Urban R,Johnston B,Johnson JR,Rahal JJ

    更新日期：2010-04-01 00:00:00

  • The clinical significance of isolation of two different organisms from the urine of patients with an indwelling catheter.

    abstract：BACKGROUND:We evaluated the clinical significance of urine cultures from patients with an indwelling urinary catheter (UC) from which 2 different pathogens were isolated. METHODS:Urine cultures from patients with a UC from which 2 different organisms were isolated were randomly divided into a control group (culture re...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2013.01.015

    authors： Wiener-Well Y,Gofman I,Assous MV,Freier-Dror Y,Yinnon AM,Lachish T

    更新日期：2013-05-01 00:00:00

  • In vitro susceptibility and synergy studies of Aspergillus species to conventional and new agents.

    abstract：:In vitro susceptibility data using a macrodilution broth method on greater than 100 isolates of Aspergillus spp. are presented. For amphotericin B (Amp B) (n = 105), 67% had minimum inhibitory concentrations (MICs) less than or equal to 2 micrograms/ml, and 90% had MICs less than or equal to 4 micrograms/ml; for 5-flu...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90053-v

    authors： Denning DW,Hanson LH,Perlman AM,Stevens DA

    更新日期：1992-01-01 00:00:00

  • Interferon gamma mRNA quantitative real-time polymerase chain reaction for the diagnosis of latent tuberculosis: a novel interferon gamma release assay.

    abstract：:The interferon gamma (IFN-γ) release assay (IGRA) is widely used as a diagnostic method for latent tuberculosis infection (LTBI). The QuantiFERON-TB Gold and QuantiFERON-TB Gold In-tube (QFT-IT) tests measure plasma IFN-γ levels using enzyme-linked immunosorbent assay (ELISA), and T-SPOT.TB counts IFN-γ-producing cell...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2012.09.015

    authors： Kim S,Kim YK,Lee H,Cho JE,Kim HY,Uh Y,Kim YM,Kim H,Cho SN,Jeon BY,Lee H

    更新日期：2013-01-01 00:00:00

  • Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods.

    abstract：:We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (less than 1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mo...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(92)90035-r

    authors： Eisenfeld L,McLaughlin JC,Mayo D,Klevjer-Anderson P,Silver H,Krause P,Anderson J,Herson V,Savidakis J,Lazar AM

    更新日期：1992-02-01 00:00:00

  • A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test.

    abstract：:Toxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient's sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.12.022

    authors： Holec-Gąsior L,Ferra B,Czechowska J,Serdiuk IE,Krzymiński K

    更新日期：2018-05-01 00:00:00

  • Comparative activity of ciprofloxacin, ofloxacin, levofloxacin, and erythromycin against Legionella species by broth microdilution and intracellular susceptibility testing in HL-60 cells.

    abstract：:Animal lung macrophages or human peripheral blood mononuclear cells have been used for testing intracellular activity of anti-Legionella antibiotics; such studies are labor intensive such that comparative antibiotic studies for the many Legionella species are few. We evaluated a human monocyte cell line (HL-60) as an ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(97)00174-0

    authors： Stout JE,Arnold B,Yu VL

    更新日期：1998-01-01 00:00:00

  • Evaluation of alternative disk diffusion methods for detecting mecA-mediated oxacillin resistance in an international collection of staphylococci: validation report from the SENTRY Antimicrobial Surveillance Program.

    abstract：:To validate the current National Committee for Clinical Laboratory Standards recommendations of the cefoxitin disk as a preferred surrogate marker to detect oxacillin resistance in staphylococcal isolates, 304 staphylococcal isolates originating from 49 sites in 16 countries in the SENTRY Antimicrobial Surveillance Pr...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2004.08.002

    authors： Pottumarthy S,Fritsche TR,Jones RN

    更新日期：2005-01-01 00:00:00

  • Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA).

    abstract：:A biotinylated single-tube nested polymerase chain reaction (PCR) assay with microwell hybridization assay (bPCR-ELISA) was developed for detection of Mycobacterium tuberculosis in clinical specimens. A total of 659 specimens (601 respiratory specimens and 58 nonrespiratory specimens) were collected for evaluation usi...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2003.11.006

    authors： Yam WC,Cheng VC,Hui WT,Wang LN,Seto WH,Yuen KY

    更新日期：2004-04-01 00:00:00

  • Nontyphoid salmonella urinary tract infections.

    abstract：:Urinary tract infection caused by nontyphoid salmonella is rare. A case is described in a patient with acquired immunodeficiency syndrome who had multiple recurrences. A review of cases in which salmonella involved the urinary tract indicates that this infection usually occurs in patients who are structurally or immun...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(87)90016-2

    authors： Eng RH,Smith SM,Kloser P

    更新日期：1987-03-01 00:00:00

  • Evaluation of a new monoclonal antibody reagent for the detection of herpes simplex virus from clinical specimens.

    abstract：:A reformulated fluorescein isothiocyanate conjugate-labeled monoclonal antibody typing reagent for herpes simplex virus (HSV) was compared for culture confirmation and typing with a similar commercially available HSV typing stain. There was complete agreement between the two stains in the confirmation of 240 cultures ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/0732-8893(93)90137-v

    authors： Johnston SL

    更新日期：1993-01-01 00:00:00

  • Endocarditis caused by Finegoldia magna (formerly Peptostreptococcus magnus): diagnosis depends on the blood culture system used.

    abstract：:We present a patient with prosthetic valve endocarditis caused by Finegoldia magna (formerly Peptostreptococcus magnus). Blood cultures in the BacT/ALERT and BACTEC 9240 system were negative. We therefore tested different blood culture systems: F. magna grew in the SEPTI-CHEK BHI-S and in the ISOLATOR, but not in the ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/s0732-8893(03)00091-9

    authors： Bassetti S,Laifer G,Goy G,Fluckiger U,Frei R

    更新日期：2003-09-01 00:00:00

  • Continued expansion of USA300-like methicillin-resistant Staphylococcus aureus (MRSA) among hospitalized patients in the United States.

    abstract：:We characterized spa types, SCCmec types, and antimicrobial resistance patterns of 516 methicillin-resistant Staphylococcus aureus (MRSA) isolates, collected between 2011 and 2014 from nares and blood cultures of United States patients. Among nares isolates, 45 spa types were observed; 29.9% were t002/SCCmec II and 30...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2017.04.016

    authors： Tickler IA,Goering RV,Mediavilla JR,Kreiswirth BN,Tenover FC,HAI Consortium.

    更新日期：2017-08-01 00:00:00

  • Reproducibility of positive results for rare pathogens on the FilmArray GI Panel.

    abstract：:Though the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for selected targets were ...

    journal_title：Diagnostic microbiology and infectious disease

    pub_type： 杂志文章

    doi：10.1016/j.diagmicrobio.2019.03.013

    authors： Hitchcock MM,Hogan CA,Budvytiene I,Banaei N

    更新日期：2019-09-01 00:00:00