Abstract：
:To evaluate the diagnostic value of immunochromatographic test (ICT) on pleural fluid in diagnosing pneumococcal empyema in children and to determine pneumococcal serotypes, 62 exudative parapneumonic effusions from Korean children were tested with culture, ICT for S. pneumoniae, pneumococcal autolysin polymerase chain reaction (PCR), and subsequent sequencing. Of the 62 patients, culture was positive in 3 patients only (4.8%). Pneumococci were identified in 13 samples (21.0%) by sequencing-confirmed PCR and ICT, respectively. When pneumococcal empyema was defined by either positive culture or sequence confirmation, the sensitivity of ICT was 76.9% (10/13) and the specificity of ICT was 93.9%. Eight of 10 patients with positive ICT and culture-negative results had a history of prior antibiotics use, whereas none of the culture-proven cases had. Serotypes of PCR-positive samples were determined by multiplex PCR assays. Multiplex PCR detected serotypes 19A (6), 1 (1), 14 (1), 34 (1), and untypable (4). ICT on pleural fluid is a relatively sensitive and highly specific method for diagnosis of pneumococcal empyema, especially in children given prior antibiotics.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Lee JH,Kim SH,Lee J,Choi EH,Lee HJdoi
10.1016/j.diagmicrobio.2011.09.025subject
Has Abstractpub_date
2012-02-01 00:00:00pages
119-24issue
2eissn
0732-8893issn
1879-0070pii
S0732-8893(11)00412-3journal_volume
72pub_type
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journal_title：Diagnostic microbiology and infectious disease
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journal_title：Diagnostic microbiology and infectious disease
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pub_type： 杂志文章
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journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,多中心研究
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