Abstract:
:Clinical isolates of C. albicans (75 strains) and other yeasts (20 strains) were evaluated for their ability to produce a carboxyl acid proteinase in an effort to assess its potential role as a virulence factor. Yeasts were categorized as to the infectious process present in the patient: (1) isolates from patients with invasive disease, (2) isolates from patients with possible invasive disease, (3) isolates from superficially infected patients and (4) isolates from noninfected, colonized patients. Yeasts were grown for 7 days in medium containing bovine serum albumin (BSA) as the sole nitrogen source. The amount of extracellular proteinase was measured at pH 3.2, using BSA as substrate. The majority (97%) of C. albicans isolates produced a detectable proteinase. Some non-C. albicans isolates produced proteinase; however, the amount of activity was generally less than for C. albicans. No correlation was found between the amount of proteolytic activity and the degree of invasiveness of the strains.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Schreiber B,Lyman CA,Gurevich J,Needham CAdoi
10.1016/0732-8893(85)90060-4subject
Has Abstractpub_date
1985-01-01 00:00:00pages
1-5issue
1eissn
0732-8893issn
1879-0070pii
0732-8893(85)90060-4journal_volume
3pub_type
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2007.04.019
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doi:10.1016/j.diagmicrobio.2009.12.022
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2003.09.008
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/s0732-8893(97)00174-0
更新日期:1998-01-01 00:00:00
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2005.03.007
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journal_title:Diagnostic microbiology and infectious disease
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abstract::Though the FilmArray GI Panel has a reported aggregate specificity and reproducibility of >97% and > 99%, respectively, the reproducibility is less understood in clinical practice. We measured the reproducibility of positive results for low-prevalence pathogens. Samples with positive results for selected targets were ...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
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journal_title:Diagnostic microbiology and infectious disease
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journal_title:Diagnostic microbiology and infectious disease
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doi:10.1016/j.diagmicrobio.2006.01.021
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journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
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abstract:BACKGROUND:Pneumococcal parapneumonic effusion seems to be increasing in children in the postvaccine era and is frequently associated with negative culture. Due to the low yield of culture, culture-independent tools are evaluated. METHODS:Culture-negative pleural fluid specimens from 38 children with parapneumonic eff...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
doi:10.1016/j.diagmicrobio.2016.07.022
更新日期:2016-10-01 00:00:00
abstract::During multilocus sequence analysis of Acinetobacter calcoaceticus-Acinetobacter baumannii complex, we identified the evidence of recent genetic recombination between 2 Acinetobacter species. While 3 isolates belonged to A. nosocomialis based on 16S rRNA, gyrB, fusA, gdhB, and rplB gene sequences, they showed close re...
journal_title:Diagnostic microbiology and infectious disease
pub_type: 杂志文章
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