Epidemiology and antimicrobial susceptibilities of wound isolates of obligate anaerobes from combat casualties.

Abstract:

:Data from recent conflicts related to war wounds and obligate anaerobes are limited. We define the epidemiology and antimicrobial susceptibility of obligate anaerobes from Iraq and Afghanistan casualties (6/2009-12/2013), as well as their association with clinical outcomes. Susceptibility against eleven antibiotics (7 classes) was tested. Overall, 59 patients had 119 obligate anaerobes identified (83 were first isolates). Obligate anaerobes were isolated 7-13 days post-injury, primarily from lower extremity wounds (43%), and were largely Bacteroides spp. (42%) and Clostridium spp. (19%). Patients with pelvic wounds were more likely to have Bacteroides spp. and concomitant resistant gram-negative aerobes. Seventy-three percent of isolates were resistant to ≥1 antimicrobials. Bacteroides spp. demonstrated the most resistance (16% of first isolates). Patients with resistant isolates had similar outcomes to those with susceptible strains. Serial recovery of isolates occurred in 15% of patients and was significantly associated with isolation of Bacteroides spp., along with resistant gram-negative aerobes.

authors

White BK,Mende K,Weintrob AC,Beckius ML,Zera WC,Lu D,Bradley W,Tribble DR,Schnaubelt ER,Murray CK,Infectious Disease Clinical Research Program Trauma Infectious Disease Outcome Study Group.

doi

10.1016/j.diagmicrobio.2015.10.010

subject

Has Abstract

pub_date

2016-02-01 00:00:00

pages

144-50

issue

2

eissn

0732-8893

issn

1879-0070

pii

S0732-8893(15)00364-8

journal_volume

84

pub_type

杂志文章
  • Effects of echinocandins on cytokine/chemokine production by human monocytes activated by infection with Candida glabrata or by lipopolysaccharide.

    abstract::Serious Candida glabrata infections, which can be difficult to treat, are often treated with echinocandins. We compared in vitro the effects of high and low concentrations of 3 echinocandins (micafungin [MCF], caspofungin [CAS], and anidulafungin [ANF]), voriconazole (VRC), and amphotericin B (AmB), singly and VRC in ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2011.11.004

    authors: Baltch AL,Lawrence DA,Ritz WJ,Andersen NJ,Bopp LH,Michelsen PB,Carlyn CJ,Smith RP

    更新日期:2012-03-01 00:00:00

  • Direct detection of methicillin-resistant Staphylococcus aureus from blood cultures using an immunochromatographic immunoassay-based MRSA rapid kit for the detection of penicillin-binding protein 2a.

    abstract::Using an EZ-Step MRSA rapid kit, a novel screening test for methicillin-resistant Staphylococcus aureus (MRSA) that detects penicillin-binding protein 2a, 34 of 36 MRSA-positive clinical blood culture samples were positive on direct testing (sensitivity, 94.4%), whereas 21 of 21 methicillin-susceptible S. aureus-posit...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2010.02.018

    authors: Shin KS,Song HG,Kim H,Yoon S,Hong SB,Koo SH,Kim J,Kim J,Roh KH

    更新日期:2010-07-01 00:00:00

  • Phenotypic and molecular characterization of Arcanobacterium haemolyticum isolated from clinical samples.

    abstract::The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological,...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2011.09.005

    authors: García-de-la-Fuente C,Ruiz De Alegría C,Cano ME,Campo AB,Unda F,Agüero J,Martínez-Martínez L

    更新日期:2012-01-01 00:00:00

  • Activity of fosfomycin when tested against US contemporary bacterial isolates.

    abstract::Fosfomycin and comparators were susceptibility tested against over 2200 contemporary clinical isolates from US medical centers. Fosfomycin was active against Enterobacterales (MIC50/90, 4/16 μg/mL), including multidrug-resistant isolates. Potent activity was exhibited against gram-positive organisms, including Staphyl...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2018.08.010

    authors: Flamm RK,Rhomberg PR,Watters AA,Sweeney K,Ellis-Grosse EJ,Shortridge D

    更新日期:2019-02-01 00:00:00

  • Evaluation of a SARS-CoV-2 lateral flow assay using the plaque reduction neutralization test.

    abstract::As new tests and technologies advance our understanding and diagnostic capabilities of the severe acute respiratory syndrome coronavirus 2 and the coronavirus disease 2019, they must be appropriately validated to make sure test performance is following manufacturer claims. In this study, we evaluated the Vazyme 2019-n...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2020.115248

    authors: Xie X,Nielsen MC,Muruato AE,Fontes-Garfias CR,Ren P

    更新日期:2021-02-01 00:00:00

  • MALDI-TOF MS identification of anaerobic bacteria: assessment of pre-analytical variables and specimen preparation techniques.

    abstract::Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a tool for identifying clinically relevant anaerobes. We evaluated the analytical performance characteristics of the Bruker Microflex with Biotyper 3.0 software system for identification of anaerobes and examined...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2014.02.007

    authors: Hsu YM,Burnham CA

    更新日期:2014-06-01 00:00:00

  • Detection of antibodies to Mycoplasma pneumoniae adhesion P1 in serum specimens from infected and non-infected subjects by immunoblotting.

    abstract::Paired serum specimens from 17 patients with Mycoplasma pneumoniae infection, as demonstrated by a rise in complement fixation (CF) antibody titer and 14 single sera, negative in the CF test, were tested for the presence of Ig A, Ig G, and Ig M antibodies to a polypeptide with a molecular weight of 170 KDa (P1) in an ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(96)00216-7

    authors: Rastawicki W,Räty R,Kleemola M

    更新日期:1996-11-01 00:00:00

  • In vitro activity of beta-lactamase inhibitors against clinical isolates of Acinetobacter species.

    abstract::Acinetobacter is an important cause of nosocomial infections, and it is often resistant to many antibiotics. In a search for alternative agents, three beta-lactamase inhibitors (sulbactam, clavulanate, and tazobactam) and five beta-lactam antibiotics (imipenem, ceftazidime, ceftriaxone, cefotaxime, and piperacillin) w...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00020-b

    authors: Suh B,Shapiro T,Jones R,Satishchandran V,Truant AL

    更新日期:1995-02-01 00:00:00

  • Bronchoalveolar lavage for the diagnosis of disseminated toxoplasmosis in AIDS patients.

    abstract::The usefulness of cell culture of bronchoalveolar lavage fluid for the diagnosis of toxoplasmosis in immunocompromised hosts has not been stressed previously. We report an acquired immunodeficiency syndrome patient with disseminated toxoplasmosis who was diagnosed by isolation of Toxoplasma gondii in cell cultures fro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(95)00142-8

    authors: Gadea I,Cuenca M,Benito N,Pereda JM,Soriano F

    更新日期:1995-08-01 00:00:00

  • Cytomegalovirus antibody detection in blood donors and mothers of very low birth weight neonates by using three serologic methods.

    abstract::We compared three serologic methods for cytomegalovirus (CMV) antibody detection and determined the CMV antibody seroprevalence of blood donors and mothers of very low birth weight (less than 1250 g) neonates in the Greater Hartford region. CMV serology was determined for 577 healthy blood donors as well as for 147 mo...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90035-r

    authors: Eisenfeld L,McLaughlin JC,Mayo D,Klevjer-Anderson P,Silver H,Krause P,Anderson J,Herson V,Savidakis J,Lazar AM

    更新日期:1992-02-01 00:00:00

  • A positive pneumococcal urinary antigen test promotes narrow spectrum antibiotic use in patients with non-invasive pneumococcal pneumonia.

    abstract:BACKGROUND:We compared changes in antibiotics in patients diagnosed with noninvasive pneumococcal pneumonia (NPP) by pneumoccocal urinary antigen tests or respiratory cultures. METHODS:We compared patients diagnosed by pneumococcal urinary antigen tests or respiratory cultures that grew Streptococcus pneumoniae. We as...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2019.114897

    authors: Banks R,Zappernick T,Wilson B,Perez F,Jump RLP

    更新日期:2020-02-01 00:00:00

  • The effect of fecal turbidity on norovirus detection by reverse transcriptase polymerase chain reaction.

    abstract::A 10-min fecal preparation results in greater specimen turbidity than a 45-min protocol, but reverse transcriptase polymerase chain reaction (RT-PCR) norovirus test sensitivity is essentially the same. Feces processed so that particle size does not exceed approximately 560 nm do not display greater norovirus RT-PCR in...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2008.08.006

    authors: Witlox KJ,Karapanagiotidis T,Bruggink LD,Marshall JA

    更新日期:2010-02-01 00:00:00

  • Comparative in vitro activity of piperacillin combined with the beta-lactamase inhibitor tazobactam (YTR 830).

    abstract::Combination with tazobactam substantially enhanced the activity of piperacillin against routine isolates of staphylococci, various Enterobacteriaceae, Acinetobacter anitratus, and Bacteroides fragilis. Tazobactam enhanced the activity of piperacillin more than fourfold against Pseudomonas aeruginosa spp. harboring eig...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(89)90082-5

    authors: Eliopoulos GM,Klimm K,Ferraro MJ,Jacoby GA,Moellering RC Jr

    更新日期:1989-11-01 00:00:00

  • Treatment of ordinary and penicillinase-producing strains of Neisseria gonorrhoeae in Mexico City.

    abstract::Since being recognized in 1976 and 1983, respectively, penicillinase-producing strains of Neisseria gonorrhoeae (PPNG) and chromosomally mediated resistance (CMRNG) have attained a worldwide distribution. The high endemicity of both types of resistance in some regions precludes the continued routine use of procaine pe...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1016/0732-8893(87)90041-1

    authors: Calderón E,Conde CJ,de la Cruz R,Narcio L,Hirata C

    更新日期:1987-09-01 00:00:00

  • Specificity of low anti-Toxoplasma IgG titers with IMx and AxSYM Toxo IgG assays.

    abstract::Low antibody titers (3 to 6 IU/mL) detected by IMx and AxSYM Toxo IgG assays in serum samples from 264 pregnant women were confirmed by the dye test and a high-sensitivity agglutination test in, respectively, 98.5% and 95.6% of cases, attesting a premunition of these patients. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(98)00060-1

    authors: Cimon B,Marty P,Morin O,Bessières MH,Marx-Chemla C,Gay-Andrieu F,Thulliez P

    更新日期:1998-09-01 00:00:00

  • The E-Test as an epidemiologic tool.

    abstract::A comparison was made of the discriminating power of the E-Test and conventional methods to distinguish different bacterial strains harboring plasmid-mediated extended-spectrum beta-lactamases. The E-Test provided quantitative data comparable to those of a broad range of concentrations in the agar dilution method, but...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(92)90095-b

    authors: Baquero F,Cantón R,Martinez-Beltrán J,Bolmström A

    更新日期:1992-07-01 00:00:00

  • Susceptibility testing of Mycobacterium tuberculosis by broth microdilution method: a rapid alternative method.

    abstract::A total of 50 clinical isolates of Mycobacterium tuberculosis were tested by the proportion method and the broth microdilution method (BMM) for primary antitubercular drugs. The results were obtained after 14 days of incubation. There was a 100% agreement for all drugs except streptomycin, which showed a 96% agreement...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.10.001

    authors: Sethi S,Mandal J,Kumar P,Kumar S,Meharwal S,Jindal SK,Sharma M

    更新日期:2007-04-01 00:00:00

  • The importance of volume of blood cultured in the detection of bacteremia and fungemia.

    abstract::An analysis was made on the basis of 5,389 isolates from 5,008 positive blood cultures of the relative yields from 10, 20, and 30 ml of blood. Average yields from cultures of 20 and 30 ml of blood were, respectively, 38 and 61% greater than that from 10 ml of blood. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(83)90039-1

    authors: Ilstrup DM,Washington JA 2nd

    更新日期:1983-06-01 00:00:00

  • Cefmetazole (CS-1170), a "new" cephamycin with a decade of clinical experience.

    abstract::In vitro and in vivo study results were reviewed from cefmetazole, a "new" parenteral cephamycin. Cefmetazole's spectrum of activity was comparable to that of second-generation cephalosporins, which includes clinical coverage of many Enterobacteriaceae, Staphylococcus spp., streptococci, Haemophilus spp., pathogenic N...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/0732-8893(89)90106-5

    authors: Jones RN

    更新日期:1989-09-01 00:00:00

  • Rapid diagnosis of tuberculosis by identification of Antigen 85 in mycobacterial culture system.

    abstract::The standard culture for identification of Mycobacterium tuberculosis takes a long time to perform. We introduce here a method for fast identification of M. tuberculosis in mycobacterial culture system. Antibodies to Antigen (Ag) 85 of M. tuberculosis were produced and subsequently used to develop enzyme-linked immuno...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2013.11.028

    authors: Phunpae P,Chanwong S,Tayapiwatana C,Apiratmateekul N,Makeudom A,Kasinrerk W

    更新日期:2014-03-01 00:00:00

  • A diagnosis of Burkholderia pseudomallei directly in a bronchoalveolar lavage by polymerase chain reaction.

    abstract::Melioidosis is an infectious disease caused by the Gram-negative bacterium Burkholderia pseudomallei. Interest in the molecular identification of B. pseudomallei has increased after its classification as a category B agent by the US Centers for Disease Control and Prevention. The present article reports a diagnosis of...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2009.05.010

    authors: Couto MS,Cordeiro Rde A,Rocha MF,Grangeiro TB,Leitão Junior NP,Bandeira Tde J,Sidrim JJ,Brilhante RS

    更新日期:2009-09-01 00:00:00

  • Risk factors for recurrence in patients with Staphylococcus aureus infections complicated by bacteremia.

    abstract::Recurrence is a common complication of Staphylococcus aureus infections. A shorter duration of antibiotic treatment for a S. aureus infection has been previously suggested as a possible risk factor for recurrence. We conducted a retrospective cohort study of patients with S. aureus infection complicated by bacteremia ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.01.021

    authors: Kreisel K,Boyd K,Langenberg P,Roghmann MC

    更新日期:2006-07-01 00:00:00

  • Oral beta-lactams in the treatment of acute bacterial rhinosinusitis.

    abstract::Acute bacterial rhinosinusitis (ABRS) is a well-known complication of viral upper respiratory tract infection and is associated with a significant socioeconomic burden. Difficulties in diagnosis, a substantial spontaneous resolution rate, and growing concerns regarding antimicrobial resistance make the proper manageme...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/j.diagmicrobio.2006.11.018

    authors: Hadley JA,Pfaller MA

    更新日期:2007-03-01 00:00:00

  • Haemophilus influenzae serotype f purulent pericarditis: a cause of death in a child with Down syndrome.

    abstract::Purulent pericarditis is a cardiac emergency that can be difficult to diagnose and can be rapidly fatal. We report the case of a child with Down syndrome and recent atrial and ventricular septal defect repair who died from Haemophilus influenzae serotype f pericarditis. ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2006.01.032

    authors: Taube JM,Hutchins GM,Carroll KC,Vricella LA,Scheel J,Halushka MK

    更新日期:2006-09-01 00:00:00

  • Evaluation of antimicrobial susceptibility for beta-lactams using the Etest method against clinical isolates from 100 medical centers in Japan (2006).

    abstract::This antimicrobial resistance surveillance study was performed in 100 medical centers. Susceptibility testing (Etest; AB BIODISK, Solna, Sweden) of 9152 strains including Escherichia coli (991 strains), Klebsiella spp. (1000 strains), Enterobacter spp. (971 strains), Citrobacter spp. (803 strains), indole-positive Pro...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2007.08.006

    authors: Ishii Y,Tateda K,Yamaguchi K,Japan Antimicrobial Resistance Surveillance Participants Group (JARS).

    更新日期:2008-02-01 00:00:00

  • Coproduction of KPC-2 and QnrB19 in Klebsiella pneumoniae ST340 isolate in Brazil.

    abstract::Few reports described the presence of bla(KPC) and qnr genes in the same isolate. This study reports the combination of bla(KPC-2) and qnrB19 genes in Klebsiella pneumoniae ST340 isolate in Brazil. These findings draw attention to this combination in ST340 isolate, which is part of the CC258, disseminated in Latin Ame...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2015.09.003

    authors: Martins WM,Almeida AC,Nicoletti AG,Cayô R,Gales AC,Alves LC,Brayner FB,Vilela MA,Morais MM

    更新日期:2015-12-01 00:00:00

  • Clinically expedient reporting of rapid diagnostic test information.

    abstract::With the development of rapid diagnostic tests in the clinical microbiology laboratory has come an awareness of the importance of rapid results reporting. Clearly, the potential clinical impact of rapid diagnostic tests is dependent on expeditious reporting. Traditional manual reporting systems are encumbered by the n...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/s0732-8893(86)80053-0

    authors: Doern GV

    更新日期:1986-03-01 00:00:00

  • Mycobacterium wolinskyi: a case series and review of the literature.

    abstract::Mycobacterium wolinskyi is an uncommonly encountered rapidly growing mycobacterium. To date, only 12 clinical cases have been reported in the literature. In this report, we describe 5 additional cases of M. wolinskyi infection seen at the Mayo Clinic in Rochester, MN, since 2009. The clinical manifestations were stern...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章,评审

    doi:10.1016/j.diagmicrobio.2011.08.005

    authors: Ariza-Heredia EJ,Dababneh AS,Wilhelm MP,Wengenack NL,Razonable RR,Wilson JW

    更新日期:2011-12-01 00:00:00

  • Screening of antibodies to varicella-zoster virus in leukemic patients: comparison of commercial methods of enzyme-immunoassay and fluoroimmunoassay.

    abstract::Commercial methods of enzyme immunoassay and fluoroimmunoassay for the detection of varicella-zoster antibodies were compared using serum samples from individuals in a program of bone marrow transplantation. The correlation was 93.4%. Enzyme immunoassay showed to be more sensitive. Both methods are applicable to the d...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/0732-8893(88)90128-9

    authors: de Ory F,Echevarria JM,Domingo CJ

    更新日期:1988-05-01 00:00:00

  • IncX3 plasmid harboring a non-Tn4401 genetic element (NTEKPC) in a hospital-associated clone of KPC-2-producing Klebsiella pneumoniae ST340/CG258.

    abstract::IncX-type plasmids have achieved clinical significance for their contribution in the dissemination of genes confering resistance to carbapenems (most blaKPC- and blaNDM-type genes) and polymyxins (mcr-type genes), both antibiotics considered last resort for multidrug-resistant Gram-negative infections. In this study, ...

    journal_title:Diagnostic microbiology and infectious disease

    pub_type: 杂志文章

    doi:10.1016/j.diagmicrobio.2017.06.022

    authors: Cerdeira LT,Cunha MPV,Francisco GR,Bueno MFC,Araujo BF,Ribas RM,Gontijo-Filho PP,Knöbl T,de Oliveira Garcia D,Lincopan N

    更新日期:2017-10-01 00:00:00