Abstract：
:Since being recognized in 1976 and 1983, respectively, penicillinase-producing strains of Neisseria gonorrhoeae (PPNG) and chromosomally mediated resistance (CMRNG) have attained a worldwide distribution. The high endemicity of both types of resistance in some regions precludes the continued routine use of procaine penicillin (APPG) as treatment for gonorrhea. In this study, we have evaluated 72/216 men with uncomplicated gonococcal urethritis at the venereal clinic in Mexico City. These men were part of a blinded randomized comparative study for treatment with group (P) penicillin having 33 patients and group (S) spectinomycin having 39. Efficacy with (P) was 24/33 (72.7%); nine failures retreated and were cured with spectinomycin. Efficacy with (S) was 35/39 (89.7%); four failures retreated and were cured with cefotaxime. We found correlation between MICs and resistance; all the strains with MICs of greater than or equal to 1.0 mcg/ml of penicillin failed to be cured, the MICs of greater than or equal to 32 mcg/ml of spectinomycin failed to be cured. The overall resistance to both regimens was 23/72 to penicillin (31.9%) (22 PPNG and one CMRNG) and 4/72 (5.5%) to spectinomycin.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Calderón E,Conde CJ,de la Cruz R,Narcio L,Hirata Cdoi
10.1016/0732-8893(87)90041-1subject
Has Abstractpub_date
1987-09-01 00:00:00pages
13-8issue
1eissn
0732-8893issn
1879-0070pii
0732-8893(87)90041-1journal_volume
8pub_type
临床试验,杂志文章,随机对照试验abstract：:We tested three fluoroquinolones (ciprofloxacin, levofloxacin, and trovafloxacin), each combined with each of four beta-lactams (cefoperazone, ceftriaxone, imipenem, and meropenem) for synergy against clinical isolates of nosocomial strains of Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Burkholderia cepa...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(98)00126-6
更新日期：1999-02-01 00:00:00
abstract：:The diagnosis of tuberculosis (TB) is difficult in children. The pediatricians are waiting for new and rapid tests that are easy to realize and that are performed better than tuberculin skin test (TST). We presented here the evaluation of QuantiFERON-TB Gold In-Tube (QFT-G IT) in the Children Hospital of Nancy. Fifty-...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.11.002
更新日期：2010-04-01 00:00:00
abstract：:A prospective study of all patients with clinically significant nosocomial bacteremia at one institution from 1994 to 1997 was performed to: (1) describe the epidemiology and microbiology of nosocomial bacteremias; (2) determine the crude mortality associated with such infections; and (3) identify independent predicto...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(00)00192-9
更新日期：2000-11-01 00:00:00
abstract：:The antimicrobial susceptibilities of 49 Clostridium difficile isolates obtained from patients with C. difficile-associated diarrhea to metronidazole, vancomycin, rifampicin, fusidic acid, doxycycline, and linezolid were determined by the disc diffusion and Etest (Biodisk, Solna, Sweden). Random amplification of polym...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2005.09.008
更新日期：2006-02-01 00:00:00
abstract：:Corynebacterium macginleyi was isolated from conjunctival swabs of a farmer suffering from purulent conjunctivitis. This species has only recently been reported in Switzerland and Germany to be exclusively isolated from ocular surfaces. This represents the first isolation of C. macginleyi in Italy indicating that its ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/s0732-8893(02)00438-8
更新日期：2002-10-01 00:00:00
abstract：:Our laboratory has developed a novel real-time polymerase chain reaction (PCR) assay for the detection of Legionella pneumophila and differentiation from other Legionella spp. in clinical and environmental samples. The 23S rRNA gene was used as a target to detect all Legionella spp., and the mip gene was targeted for ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2008.05.004
更新日期：2008-10-01 00:00:00
abstract：:The examination of fecal mucus for detecting yeast cells of Histoplasma capsulatum has proved to be a useful tool for diagnosing disseminated histoplasmosis in paediatric patients in a study of 13 cases carried out in Ecuador. ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.10.016
更新日期：2007-04-01 00:00:00
abstract：:Early diagnosis of leptospirosis in humans is critical with regard to initiation of appropriate treatment; however, the gold standard serological test cannot detect antibodies until nearly a week after symptom onset. PCR has been shown to be sensitive and specific in the early phase of leptospirosis. Previously, we de...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2015.03.024
更新日期：2015-07-01 00:00:00
abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) has emerged as an important pathogen. Successful detection starts with the selection of an appropriate specimen collection device. The Copan ESwab, a new swab, was compared with a commonly used Copan Venturi swab for the recovery of MRSA. In vitro assessment was perfo...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2009.06.015
更新日期：2009-10-01 00:00:00
abstract：:Sparfloxacin is a piperazinyl, cyclopropyl-fluoroquinolone with broad-spectrum antibacterial activity. Compared to other quinolones, sparfloxacin displays improved activity against a variety of pathogens including Staphylococcus, Streptococcus, Enterococcus, Chlamydia, Mycoplasma, Ureaplasma, and Mycobacteria species....
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/s0732-8893(96)00121-6
更新日期：1996-06-01 00:00:00
abstract：:We conducted a series of polymerase chain reactions (PCRs) in order to detect bacteria (7 species) and viruses (17 species) in middle ear fluid (MEF) and nasopharynx (Nph) of children with acute otitis media (AOM; n=179). Bacterial and viral nucleic acids were detected in MEF of 78.8% and 14.5% patients, respectively....
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2016.02.010
更新日期：2016-05-01 00:00:00
abstract：:Borrelia burgdorferi sensu lato (s. l.), the agent of Lyme disease, is distributed widely worldwide. A large number of polymerase chain reaction (PCR) methods have been developed and used for detection of B. burgdorferi s. l. However, there is a lack of a reference standard because of the genetic diversity of the B. b...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2012.02.016
更新日期：2012-05-01 00:00:00
abstract：:Ceftaroline fosamil resulted in higher cure rates than ceftriaxone in patients with community-acquired bacterial pneumonia in 2 randomized trials (FOCUS 1 and FOCUS 2). The present analysis examines the subgroup of patients with Streptococcus pneumoniae infection to determine whether the apparent difference in cure ra...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.diagmicrobio.2012.12.002
更新日期：2013-03-01 00:00:00
abstract：:This study assessed the demographics, antimicrobial susceptibility, and molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and health care-associated MRSA (HA-MRSA) in Canadian hospitals between 2007 and 2009. Among 3589 S. aureus, 889 (24.8%) were MRSA; 224 (25.2%) we...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2010.10.028
更新日期：2011-03-01 00:00:00
abstract：:A reformulated fluorescein isothiocyanate conjugate-labeled monoclonal antibody typing reagent for herpes simplex virus (HSV) was compared for culture confirmation and typing with a similar commercially available HSV typing stain. There was complete agreement between the two stains in the confirmation of 240 cultures ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/0732-8893(93)90137-v
更新日期：1993-01-01 00:00:00
abstract：:We report on a scrub typhus patient who contracted meningoencephalitis during doxycycline administration. This is the first case of scrub typhus in which doxycycline concentrations were measured in serum and cerebrospinal fluid. Clinicians should be alerted to the possibility that meningoencephalitis can occur because...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2010.10.005
更新日期：2011-03-01 00:00:00
abstract：:Recent reports suggest that false-positive rifampicin resistance may be assigned by the Xpert MTB/RIF assay. We analysed 169 specimens using the MTB/RIF assay. Using culture as the gold standard, we found that the assay had 100% sensitivity and specificity for detecting M. tuberculosis. However, we found that the assa...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2012.06.013
更新日期：2012-10-01 00:00:00
abstract：:The objective of the study is to compare the performance of conventional fluorescence microscopy (CFM) and light-emitting diode (LED) fluorescence microscopy (FM) for detection of acid-fast bacilli (AFB) in clinical samples. We included AFB smears, stained using the auramine O method and blindly examined with both CFM...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2013.03.023
更新日期：2013-07-01 00:00:00
abstract：:At 24 h, sub-MIC doripenem and levofloxacin showed synergy against 21 of 25 Pseudomonas aeruginosa strains, sub-MIC doripenem and amikacin against 22 isolates, and sub-MIC doripenem and colistin against 19 isolates. Of 25 Acinetobacter baumannii strains, sub-MIC doripenem and levofloxacin showed synergy against 11 str...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2010.01.004
更新日期：2010-06-01 00:00:00
abstract：:The mechanism of macrolide resistance was investigated in 251 Streptococcus pneumoniae isolates with reduced susceptibility to erythromycin (erythromycin-resistant S. pneumoniae [ERSP]) collected during the period from 2000 to 2004 in Korea. Among these strains, erm(B) was the most prevalent pneumococcal macrolide res...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2008.10.002
更新日期：2009-02-01 00:00:00
abstract：:Cefotaxime, the first widely used "third-generation" cephalosporin, has established efficacy against a variety of serious bacterial pathogens. Some of the initial clinical studies in the United States using this agent employed large doses of the compound, up to 12 g/day, for adults. In contrast, however, initial Europ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/0732-8893(95)00103-h
更新日期：1995-05-01 00:00:00
abstract：:Early diagnosis of influenza infection is needed to optimize the benefit of prescribing antiviral drugs. However, the accuracy of rapid tests is highly variable. This study evaluated the performance of Directigen flu A+B enzyme immunoassay (EIA) and direct fluorescent assay (DFA) during the 2004-2005 influenza season....
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2007.03.011
更新日期：2007-08-01 00:00:00
abstract：:A multianalyte Dot-enzyme-linked immunosorbent assay (Dot-ELISA-Multi) with Trypanosoma cruzi epimastigote alkaline extract (EAE), trypomastigote excreted-secreted antigen (TESA), recombinant protein derived from 19-kDa C-terminal region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1(19)), Plasmodium falc...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2006.12.011
更新日期：2007-06-01 00:00:00
abstract：:The clinical features of abdominal tuberculosis (TB) are non-specific and establishing a diagnosis remains a challenge. A delay in diagnosis is likely to increase the morbidity in these patients. We developed a multiplex polymerase chain reaction (PCR) using 16SrRNA, IS6110, and devR, and evaluated it in comparison wi...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2013.02.022
更新日期：2013-05-01 00:00:00
abstract：:Legionella pneumophila is responsible for Legionnaires' disease (LD). Its detection in both environmental and clinical samples is mainly performed by culture plate method which requires up to 10days to obtain results. Nowadays, there are commercial antibodies against this bacterium, but they have not been tested again...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2017.11.005
更新日期：2018-02-01 00:00:00
abstract：:A commercially prepared dried-broth microdilution panel (Sensititre, TREK Diagnostic Systems, Cleveland, OH) was compared with a reference frozen-broth microdilution panel for antifungal susceptibility testing of 728 clinical isolates of Candida spp. and 98 clinical isolates of Cryptococcus neoformans. The antifungal ...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2004.06.013
更新日期：2004-10-01 00:00:00
abstract：:Acute otitis media (AOM) is an important infectious disease among children throughout the world. The management of AOM is complicated by difficulties in diagnosis, spontaneous resolution, altered microbiology after pneumococcal conjugate vaccine use, and expanding antimicrobial resistance. The recent introduction of t...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/j.diagmicrobio.2007.01.011
更新日期：2007-03-01 00:00:00
abstract：:Polymerase chain reaction (PCR) has been widely used due to its high specificity, sensitivity, and rapid turn-around time. However, inhibitory factors may be co-extracted with the target nucleic acid that will hinder the performance of PCR. In this study, DNA extraction methods for Mycobacterium avium subsp. paratuber...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2003.08.007
更新日期：2004-01-01 00:00:00
abstract：:Rhinocladiella, a genus of black yeast-like fungi, is related to many infections in humans, including not only mild cutaneous lesions but also fatal brain infections. However, endophthalmitis caused by Rhinocladiella has never been reported by far. Herein, we present the first case of endophthalmitis due to Rhinocladi...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章
doi：10.1016/j.diagmicrobio.2015.05.014
更新日期：2015-09-01 00:00:00
abstract：:The recent development of nucleic acid amplification methodologies has markedly improved our ability to detect very low levels of specific nucleic acids. Amplification techniques have been combined with product detection systems that are designed for high throughput and are automatable. These developments are drastica...
journal_title：Diagnostic microbiology and infectious disease
pub_type： 杂志文章,评审
doi：10.1016/0732-8893(93)90016-z
更新日期：1993-02-01 00:00:00
