Abstract:
:A multianalyte Dot-enzyme-linked immunosorbent assay (Dot-ELISA-Multi) with Trypanosoma cruzi epimastigote alkaline extract (EAE), trypomastigote excreted-secreted antigen (TESA), recombinant protein derived from 19-kDa C-terminal region of the Plasmodium vivax merozoite surface protein 1 (PvMSP1(19)), Plasmodium falciparum Zwittergent extract (Pf-Zw), and Treponema pallidum Zwittergent extract (Tp-Zw) was standardized and evaluated as a method for surveying IgG-specific antibodies in Chagas disease, malaria, and syphilis in a single test. The study was carried out on serum samples from 52 patients with chronic Chagas disease, 103 individuals with current (parasitemic) or past malaria (aparasitemic), 43 patients with syphilis, 21 individuals with heterologous antibodies, and 100 blood donors. Dot-ELISA-Multi yielded 99% specificity for Chagas disease and 100% for malaria and syphilis. The test sensitivity was 100% for chronic Chagas disease, 88% for syphilis, 90% for P. vivax, and 47% for P. falciparum. In past malaria individuals, positivity was 92%. Therefore, Dot-ELISA-Multi can be useful under field conditions where laboratory facilities and resources are scarce, for small-scale epidemiologic studies.
journal_name
Diagn Microbiol Infect Disjournal_title
Diagnostic microbiology and infectious diseaseauthors
Coelho JS,Soares IS,Lemos EA,Jimenez MC,Kudó ME,Moraes SL,Ferreira AW,Sanchez MCdoi
10.1016/j.diagmicrobio.2006.12.011subject
Has Abstractpub_date
2007-06-01 00:00:00pages
223-30issue
2eissn
0732-8893issn
1879-0070pii
S0732-8893(06)00518-9journal_volume
58pub_type
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